P43165 (CAH5A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 5A, mitochondrial
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase VA
Carbonic anhydrase VA
Short name=CA-VA

Gene names

Name:	Ca5a
Synonyms:	Ca5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversible hydration of carbon dioxide. Low activity.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Enzyme regulation	Inhibited by acetazolamide By similarity.
Subcellular location	Mitochondrion.
Tissue specificity	High in liver, also detected in heart, lung, kidney, spleen and intestine.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay Ref.1. Source: RGD
Molecular function	carbonate dehydratase activity Inferred from direct assay Ref.1. Source: RGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion Ref.1
	Chain	35 – 304	270	Carbonic anhydrase 5A, mitochondrial		PRO_0000004236
Regions
	Region	234 – 235	2	Substrate binding By similarity
Sites
	Active site	163	1	By similarity
	Active site	166	1	Probable
	Metal binding	129	1	Zinc; catalytic By similarity
	Metal binding	131	1	Zinc; catalytic By similarity
	Metal binding	154	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43165 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C997805D690F95D0	FASTA	304	34,499
	10 20 30 40 50 60 MLRAKMLGRG PYKPLAILRH MGPLCATRPQ HWRFQHSYAE KHSNCARHPL WTGPVSSPGG 70 80 90 100 110 120 TQQSPINIQW TDSVYDPKLA PLRVSYDAAS CRYLWNTGYF FQVEFDDSCE ESGISGGPLG 130 140 150 160 170 180 NHYRLKQFHF HWGATDEWGS EHMVDGHAYP AELHLVHWNS MKYENYKKAT TGENGLAVIG 190 200 210 220 230 240 VFLKLGAHHE ALQRLVDILP EVRHKDTQVT MGPFDPSCLL PACRDYWTYP GSLTTPPLAE 250 260 270 280 290 300 SVTWIVHKMP IEVSPSQLST FRTLLFSGRG EDEEVMVNNF RPLQPLRGRN VRSSFQVPRV GTKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial carbonic anhydrase (isozyme V) in mouse and rat: cDNA cloning, expression, subcellular localization, processing, and tissue distribution." Nagao Y., Srinivasan M., Platero J.S., Svendrowski M., Waheed A., Sly W.S. Proc. Natl. Acad. Sci. U.S.A. 91:10330-10334(1994) [PubMed: 7937950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 35-46. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12268 mRNA. Translation: AAA50832.1. BC088147 mRNA. Translation: AAH88147.1.
IPI	IPI00197535.
PIR	I59261.
RefSeq	NP_062166.1. NM_019293.1.
UniGene	Rn.9988.
3D structure databases
ProteinModelPortal	P43165.
SMR	P43165. Positions 60-295.
ModBase	Search...
Proteomic databases
PRIDE	P43165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025848; ENSRNOP00000025848; ENSRNOG00000019098.
GeneID	54233.
KEGG	rno:54233.
UCSC	NM_019293. rat.
Organism-specific databases
CTD	12352.
RGD	2243. Ca5a.
Phylogenomic databases
eggNOG	maNOG05904.
GeneTree	ENSGT00560000076828.
HOVERGEN	HBG002837.
InParanoid	P43165.
OMA	SYDAASC.
OrthoDB	EOG4FR0RX.
PhylomeDB	P43165.
Gene expression databases
ArrayExpress	P43165.
Genevestigator	P43165.
GermOnline	ENSRNOG00000019098. Rattus norvegicus.
Family and domain databases
InterPro	IPR001148. a_carbonic_anhydrase. IPR023561. Carbonic_anhydrase_a-class. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018437. Carbonic_anhydrase_CA5_mt. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
KO	K01672.
PANTHER	PTHR18952:SF25. Carbonic_anhydrase_CA5_mt. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
SMART	SM01057. Carb_anhydrase. 1 hit. [Graphical view]
SUPFAM	SSF51069. Euk_COanhd. 1 hit.
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610670.

Entry information

Entry name

CAH5A_RAT

Accession

Primary (citable) accession number: P43165

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program