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Protein

Microbial collagenase

Gene
N/A
Organism
Vibrio alginolyticus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi477Zinc; catalyticPROSITE-ProRule annotation1
Active sitei478PROSITE-ProRule annotation1
Metal bindingi481Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processCollagen degradation
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM09.001

Names & Taxonomyi

Protein namesi
Recommended name:
Microbial collagenase (EC:3.4.24.3)
OrganismiVibrio alginolyticus
Taxonomic identifieri663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002867622 – 75Add BLAST54
ChainiPRO_000002867776 – 814Microbial collagenaseAdd BLAST739

Post-translational modificationi

Proteolytic cleavage might yield three different active forms.

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiP43154

Structurei

3D structure databases

ProteinModelPortaliP43154
SMRiP43154
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini609 – 697PKDPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the peptidase M9A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107MM9 Bacteria
ENOG410ZRXP LUCA

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR013661 Peptidase_M9_N_dom
IPR002169 Peptidase_M9A/M9B
IPR022409 PKD/Chitinase_dom
IPR000601 PKD_dom
IPR035986 PKD_dom_sf
PfamiView protein in Pfam
PF01752 Peptidase_M9, 1 hit
PF08453 Peptidase_M9_N, 1 hit
PF00801 PKD, 1 hit
PRINTSiPR00931 MICOLLPTASE
SMARTiView protein in SMART
SM00089 PKD, 1 hit
SUPFAMiSSF49299 SSF49299, 1 hit
PROSITEiView protein in PROSITE
PS50093 PKD, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKILSVAI ATTLTSTGVF ALSEPVSQVT EQHAHSAHTH GVEFNRVEYQ
60 70 80 90 100
PTATLPIQPS KATRVQSLES LDESSTACDL EALVTESSNQ LISEILSQGA
110 120 130 140 150
TCVNQLFSAE SRIQESVFSS DHMYNIAKHT TTLAKGYTGG GSDELETLFL
160 170 180 190 200
YLRAGYYAEF YNDNISFIEW VTPAVKESVD AFVNTASFYE NSDRHGKVLS
210 220 230 240 250
EVIITMDSAG LQHAYLPQVT QWLTRWNDQY AQHWYMRNAV NGVFTILFGG
260 270 280 290 300
QWNEQFVQII GNQTDLAKAL GDFALRASSI GAEDEFMAAN AGRELGRLTK
310 320 330 340 350
YTGNASSVVK SQLSRIFEQY EMYGRGDAVW LAAADTASYY ADCSEFGICN
360 370 380 390 400
FETELKGLVL SQTYTCSPTI RILSQNMTQE QHAAACSKMG YEEGYFHQSL
410 420 430 440 450
ETGEQPVKDD HNTQLQVNIF DSSTDYGKYA GPIFDISTDN GGMYLEGDPS
460 470 480 490 500
QPGNIPNFIA YEASYANADH FVWNLEHEYV HYLDGRFDLY GGFSHPTEKI
510 520 530 540 550
VWWSEGIAEY VAQENDNQAA LETILDGSTY TLSEIFETTY DGFDVDRIYR
560 570 580 590 600
WGYLAVRFMF ENHKDDVNQM LVETRQGNWI NYKATITQWA NLYQSEFEQW
610 620 630 640 650
QQTLVSNGAP NAVITANSKG KVGESITFSS ENSTDPNGKI VSVLWDFGDG
660 670 680 690 700
STSTQTKPTH QYGSEGEYSV SLSVTDSEGL TATATHTVVI SALGGNDTLP
710 720 730 740 750
QDCAVQSKVS GGRLTAGEPV CLANQQTIWL SVPAVNESSN LAITTGNGTG
760 770 780 790 800
NLKLEYSNSG WPDDTNLHGW SDNIGNGECI TLSNQSNYWG YVKVSGDFEN
810
AAIVVDFDAQ KCRQ
Length:814
Mass (Da):89,963
Last modified:November 1, 1995 - v1
Checksum:iE0E19C0884D2185E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62635 Genomic DNA Translation: CAA44501.1
PIRiS19658

Entry informationi

Entry nameiCOLA_VIBAL
AccessioniPrimary (citable) accession number: P43154
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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