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Protein

Microbial collagenase

Gene
N/A
Organism
Vibrio alginolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi477 – 4771Zinc; catalyticPROSITE-ProRule annotation
Active sitei478 – 4781PROSITE-ProRule annotation
Metal bindingi481 – 4811Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM09.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Microbial collagenase (EC:3.4.24.3)
OrganismiVibrio alginolyticus
Taxonomic identifieri663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 7554PRO_0000028676Add
BLAST
Chaini76 – 814739Microbial collagenasePRO_0000028677Add
BLAST

Post-translational modificationi

Proteolytic cleavage might yield three different active forms.

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi674977.VMC_41110.

Structurei

3D structure databases

ProteinModelPortaliP43154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini609 – 69789PKDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M9A family.Curated
Contains 1 PKD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107MM9. Bacteria.
ENOG410ZRXP. LUCA.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PRINTSiPR00931. MICOLLPTASE.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKILSVAI ATTLTSTGVF ALSEPVSQVT EQHAHSAHTH GVEFNRVEYQ
60 70 80 90 100
PTATLPIQPS KATRVQSLES LDESSTACDL EALVTESSNQ LISEILSQGA
110 120 130 140 150
TCVNQLFSAE SRIQESVFSS DHMYNIAKHT TTLAKGYTGG GSDELETLFL
160 170 180 190 200
YLRAGYYAEF YNDNISFIEW VTPAVKESVD AFVNTASFYE NSDRHGKVLS
210 220 230 240 250
EVIITMDSAG LQHAYLPQVT QWLTRWNDQY AQHWYMRNAV NGVFTILFGG
260 270 280 290 300
QWNEQFVQII GNQTDLAKAL GDFALRASSI GAEDEFMAAN AGRELGRLTK
310 320 330 340 350
YTGNASSVVK SQLSRIFEQY EMYGRGDAVW LAAADTASYY ADCSEFGICN
360 370 380 390 400
FETELKGLVL SQTYTCSPTI RILSQNMTQE QHAAACSKMG YEEGYFHQSL
410 420 430 440 450
ETGEQPVKDD HNTQLQVNIF DSSTDYGKYA GPIFDISTDN GGMYLEGDPS
460 470 480 490 500
QPGNIPNFIA YEASYANADH FVWNLEHEYV HYLDGRFDLY GGFSHPTEKI
510 520 530 540 550
VWWSEGIAEY VAQENDNQAA LETILDGSTY TLSEIFETTY DGFDVDRIYR
560 570 580 590 600
WGYLAVRFMF ENHKDDVNQM LVETRQGNWI NYKATITQWA NLYQSEFEQW
610 620 630 640 650
QQTLVSNGAP NAVITANSKG KVGESITFSS ENSTDPNGKI VSVLWDFGDG
660 670 680 690 700
STSTQTKPTH QYGSEGEYSV SLSVTDSEGL TATATHTVVI SALGGNDTLP
710 720 730 740 750
QDCAVQSKVS GGRLTAGEPV CLANQQTIWL SVPAVNESSN LAITTGNGTG
760 770 780 790 800
NLKLEYSNSG WPDDTNLHGW SDNIGNGECI TLSNQSNYWG YVKVSGDFEN
810
AAIVVDFDAQ KCRQ
Length:814
Mass (Da):89,963
Last modified:November 1, 1995 - v1
Checksum:iE0E19C0884D2185E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62635 Genomic DNA. Translation: CAA44501.1.
PIRiS19658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62635 Genomic DNA. Translation: CAA44501.1.
PIRiS19658.

3D structure databases

ProteinModelPortaliP43154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi674977.VMC_41110.

Protein family/group databases

MEROPSiM09.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107MM9. Bacteria.
ENOG410ZRXP. LUCA.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PRINTSiPR00931. MICOLLPTASE.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOLA_VIBAL
AccessioniPrimary (citable) accession number: P43154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.