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Protein

Microbial collagenase

Gene

colA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi502Zinc; catalyticPROSITE-ProRule annotation1
Active sitei503PROSITE-ProRule annotation1
Metal bindingi506Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Microbial collagenase (EC:3.4.24.3)
Alternative name(s):
120 kDa collagenase
Gene namesi
Name:colA
Ordered Locus Names:CPE0173
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2802.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39Sequence analysisAdd BLAST39
PropeptideiPRO_000002867440 – 861 PublicationAdd BLAST47
ChainiPRO_000002867587 – 1104Microbial collagenaseAdd BLAST1018

Keywords - PTMi

Zymogen

Interactioni

Chemistry databases

BindingDBiP43153.

Structurei

3D structure databases

ProteinModelPortaliP43153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini774 – 862PKDPROSITE-ProRule annotationAdd BLAST89

Sequence similaritiesi

Belongs to the peptidase M9B family.Curated
Contains 1 PKD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000055596.
KOiK01387.
OMAiVFHGINT.
OrthoDBiPOG091H02X9.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PRINTSiPR00931. MICOLLPTASE.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKNLKRGEL TKLKLVERWS ATFTLAAFIL FNSSFKVLAA DKKVENSNNG
60 70 80 90 100
QITREINADQ ISKTELNNEV ATDNNRPLGP SIAPSRARNN KIYTFDELNR
110 120 130 140 150
MNYSDLVELI KTISYENVPD LFNFNDGSYT FFSNRDRVQA IIYGLEDSGR
160 170 180 190 200
TYTADDDKGI PTLVEFLRAG YYLGFYNKQL SYLNTPQLKN ECLPAMKAIQ
210 220 230 240 250
YNSNFRLGTK AQDGVVEALG RLIGNASADP EVINNCIYVL SDFKDNIDKY
260 270 280 290 300
GSNYSKGNAV FNLMKGIDYY TNSVIYNTKG YDAKNTEFYN RIDPYMERLE
310 320 330 340 350
SLCTIGDKLN NDNAWLVNNA LYYTGRMGKF REDPSISQRA LERAMKEYPY
360 370 380 390 400
LSYQYIEAAN DLDLNFGGKN SSGNDIDFNK IKADAREKYL PKTYTFDDGK
410 420 430 440 450
FVVKAGDKVT EEKIKRLYWA SKEVKAQFMR VVQNDKALEE GNPDDILTVV
460 470 480 490 500
IYNSPEEYKL NRIINGFSTD NGGIYIENIG TFFTYERTPE ESIYTLEELF
510 520 530 540 550
RHEFTHYLQG RYVVPGMWGQ GEFYQEGVLT WYEEGTAEFF AGSTRTDGIK
560 570 580 590 600
PRKSVTQGLA YDRNNRMSLY GVLHAKYGSW DFYNYGFALS NYMYNNNMGM
610 620 630 640 650
FNKMTNYIKN NDVSGYKDYI ASMSSDYGLN DKYQDYMDSL LNNIDNLDVP
660 670 680 690 700
LVSDEYVNGH EAKDINEITN DIKEVSNIKD LSSNVEKSQF FTTYDMRGTY
710 720 730 740 750
VGGRSQGEEN DWKDMNSKLN DILKELSKKS WNGYKTVTAY FVNHKVDGNG
760 770 780 790 800
NYVYDVVFHG MNTDTNTDVH VNKEPKAVIK SDSSVIVEEE INFDGTESKD
810 820 830 840 850
EDGEIKAYEW DFGDGEKSNE AKATHKYNKT GEYEVKLTVT DNNGGINTES
860 870 880 890 900
KKIKVVEDKP VEVINESEPN NDFEKANQIA KSNMLVKGTL SEEDYSDKYY
910 920 930 940 950
FDVAKKGNVK ITLNNLNSVG ITWTLYKEGD LNNYVLYATG NDGTVLKGEK
960 970 980 990 1000
TLEPGRYYLS VYTYDNQSGT YTVNVKGNLK NEVKETAKDA IKEVENNNDF
1010 1020 1030 1040 1050
DKAMKVDSNS KIVGTLSNDD LKDIYSIDIQ NPSDLNIVVE NLDNIKMNWL
1060 1070 1080 1090 1100
LYSADDLSNY VDYANADGNK LSNTCKLNPG KYYLCVYQFE NSGTGNYIVN

LQNK
Length:1,104
Mass (Da):125,936
Last modified:January 31, 2002 - v2
Checksum:iF4B7377194ED021C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38L → F in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti722I → M in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti748G → E in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti945V → E in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti970T → A in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti987A → E in BAA02941 (PubMed:8282691).Curated1
Sequence conflicti1098I → T (PubMed:8282691).Curated1
Sequence conflicti1098I → T (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13791 Genomic DNA. Translation: BAA02941.1.
BA000016 Genomic DNA. Translation: BAB79879.1.
D50309 Genomic DNA. Translation: BAA08848.1.
PIRiA36866.
RefSeqiWP_011009653.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB79879; BAB79879; BAB79879.
KEGGicpe:CPE0173.
PATRICi19494226. VBICloPer59675_0230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13791 Genomic DNA. Translation: BAA02941.1.
BA000016 Genomic DNA. Translation: BAB79879.1.
D50309 Genomic DNA. Translation: BAA08848.1.
PIRiA36866.
RefSeqiWP_011009653.1. NC_003366.1.

3D structure databases

ProteinModelPortaliP43153.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP43153.
ChEMBLiCHEMBL2802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB79879; BAB79879; BAB79879.
KEGGicpe:CPE0173.
PATRICi19494226. VBICloPer59675_0230.

Phylogenomic databases

HOGENOMiHOG000055596.
KOiK01387.
OMAiVFHGINT.
OrthoDBiPOG091H02X9.

Family and domain databases

Gene3Di2.60.40.670. 1 hit.
InterProiIPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamiPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
[Graphical view]
PRINTSiPR00931. MICOLLPTASE.
SMARTiSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMiSSF49299. SSF49299. 1 hit.
PROSITEiPS50093. PKD. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOLA_CLOPE
AccessioniPrimary (citable) accession number: P43153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 31, 2002
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.