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P43152

- HPRT_LEIDO

UniProt

P43152 - HPRT_LEIDO

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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene
N/A
Organism
Leishmania donovani
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661GMPBy similarity
Active sitei129 – 1291Proton acceptorBy similarity
Binding sitei157 – 1571GMPBy similarity
Metal bindingi185 – 1851MagnesiumBy similarity
Binding sitei185 – 1851GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi125 – 1339GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
OrganismiLeishmania donovani
Taxonomic identifieri5661 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139593Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP43152.
SMRiP43152. Positions 20-201.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00760.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43152-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNSAKSPSG PVGDEGRRNY PMSAHTLVTQ EQVWAATAKC AKKIAEDYRS
60 70 80 90 100
FKLTTDNPLY LLCVLKGSFI FTADLARFLA DEGVPVKVEF ICASSYGTGV
110 120 130 140 150
ETSGQVRMLL DVRDSVENRH ILIVEDIVDS AITLQYLMRF MLAKKPASLK
160 170 180 190 200
TVVLLDKPSG RKVEVLVDYP VITIPHAFVI GYGMDYAESY RELRDICVLK
210
KEYYEKPESK V
Length:211
Mass (Da):23,612
Last modified:November 1, 1995 - v1
Checksum:i7802A85B00CA2190
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25412 Genomic DNA. Translation: AAB00074.1.
RefSeqiXP_003860615.1. XM_003860567.1.

Genome annotation databases

GeneIDi13386422.
KEGGildo:LDBPK_210980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25412 Genomic DNA. Translation: AAB00074.1 .
RefSeqi XP_003860615.1. XM_003860567.1.

3D structure databases

ProteinModelPortali P43152.
SMRi P43152. Positions 20-201.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13386422.
KEGGi ldo:LDBPK_210980.

Phylogenomic databases

KOi K00760.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of the hypoxanthine-guanine phosphoribosyltransferase from Leishmania donovani."
    Allen T.E., Hwang H.Y., Jardim A., Olafson R., Ullman B.
    Mol. Biochem. Parasitol. 73:133-143(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MHOM/SD/62/1S.

Entry informationi

Entry nameiHPRT_LEIDO
AccessioniPrimary (citable) accession number: P43152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3