Neuronal acetylcholine receptor subunit alpha-9 precursor

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P43144 (ACHA9_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neuronal acetylcholine receptor subunit alpha-9

Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-9
Short name=NACHR alpha-9

Gene names

Name:	Chrna9
Synonyms:	Acra9

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion. Ref.1 Ref.3 Ref.4 Ref.6 Ref.8
Subunit structure	Can form homo- or hetero-oligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Detected in the nasal epithelium, in the outer hair cells of the cochlea, in the pars tuberalis of the hypophysis, and in the developing muscle of the tongue. Also expressed in the neurons of dorsal root ganglia. Ref.1 Ref.5 Ref.7
Miscellaneous	The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-9/CHRNA9 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of mechanical stimulus involved in sensory perception of sound Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara inner ear morphogenesis Inferred from electronic annotation. Source: Compara sensory perception of sound Inferred from expression pattern Ref.1. Source: RGD
Cellular_component	acetylcholine-gated channel complex Inferred from direct assay Ref.7. Source: RGD cell junction Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetylcholine-activated cation-selective channel activity Inferred from direct assay Ref.1. Source: RGD calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

Potential

Chain

26 – 479

454

Neuronal acetylcholine receptor subunit alpha-9

PRO_0000000372

Regions

Topological domain

26 – 237

212

Extracellular Potential

Transmembrane

238 – 262

Helical; Potential

Transmembrane

269 – 287

Helical; Potential

Transmembrane

302 – 323

Helical; Potential

Topological domain

324 – 457

134

Cytoplasmic Potential

Transmembrane

458 – 476

Helical; Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

170

N-linked (GlcNAc...) Potential

Disulfide bond

155 ↔ 169

By similarity

Disulfide bond

219 ↔ 220

Associated with receptor activation By similarity

Experimental info

Sequence conflict

425

T → A in AAA56720. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P43144 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: CBB3E15E9DF68837
Show »

FASTA

479

54,526

        10         20         30         40         50         60 
MNRPHSCLSF CWMYFAASGI RAVETANGKY AQKLFSDLFE DYSSALRPVE DTDAVLNVTL 

        70         80         90        100        110        120 
QVTLSQIKDM DERNQILTAY LWIRQTWHDA YLTWDRDQYD RLDSIRIPSD LVWRPDIVLY 

       130        140        150        160        170        180 
NKADDESSEP VNTNVVLRYD GLITWDSPAI TKSSCVVDVT YFPFDSQQCN LTFGSWTYNG 

       190        200        210        220        230        240 
NQVDIFNALD SGDLSDFIED VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY 

       250        260        270        280        290        300 
IVNLLIPCVL ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG 

       310        320        330        340        350        360 
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWAKVV ILKYMSRILF VYDVGESCLS 

       370        380        390        400        410        420 
PRHSQEPEQV TKVYSKLPES NLKTSRNKDL SRKKEVRKLL KNDLGYQGGI PQNTDSYCAR 

       430        440        450        460        470 
YEALTKNIEY IAKCLKDHKA TNSKGSEWKK VAKVIDRFFM WIFFAMVFVM TVLIIARAD

« Hide

References

[1]	"Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells." Elgoyhen A.B., Johnson D.S., Boulter J., Vetter D.E., Heinemann S.F. Cell 79:705-715(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Olfactory epithelium.
[2]	Groot-Kormelink P.J. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"High calcium permeability and calcium block of the alpha9 nicotinic acetylcholine receptor." Katz E., Verbitsky M., Rothlin C.V., Vetter D.E., Heinemann S.F., Elgoyhen A.B. Hear. Res. 141:117-128(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Mixed nicotinic-muscarinic properties of the alpha9 nicotinic cholinergic receptor." Verbitsky M., Rothlin C.V., Katz E., Elgoyhen A.B. Neuropharmacology 39:2515-2524(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells." Elgoyhen A.B., Vetter D.E., Katz E., Rothlin C.V., Heinemann S.F., Boulter J. Proc. Natl. Acad. Sci. U.S.A. 98:3501-3506(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CHRNA10, TISSUE SPECIFICITY.
[6]	"The alpha9alpha10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations." Weisstaub N., Vetter D.E., Elgoyhen A.B., Katz E. Hear. Res. 167:122-135(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHRNA10.
[7]	"Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons." Lips K.S., Pfeil U., Kummer W. Neuroscience 115:1-5(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Pharmacological properties of alpha 9 alpha 10 nicotinic acetylcholine receptors revealed by heterologous expression of subunit chimeras." Baker E.R., Zwart R., Sher E., Millar N.S. Mol. Pharmacol. 65:453-460(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHRNA10.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12336 mRNA. Translation: AAA56720.1. AY574257 mRNA. Translation: AAS90353.1.
IPI	IPI00197478.
PIR	A55382.
RefSeq	NP_075219.1. NM_022930.1.
UniGene	Rn.54463.
3D structure databases
ProteinModelPortal	P43144.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000003382.
PTM databases
PhosphoSite	P43144.
Protocols and materials databases
DNASU	65024.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000003382; ENSRNOP00000003382; ENSRNOG00000002484.
GeneID	65024.
KEGG	rno:65024.
UCSC	RGD:621534. rat.
Organism-specific databases
CTD	55584.
RGD	621534. Chrna9.
Phylogenomic databases
eggNOG	NOG306809.
GeneTree	ENSGT00630000089708.
HOGENOM	HOG000006756.
HOVERGEN	HBG003756.
InParanoid	P43144.
KO	K04810.
OMA	CAQYKVL.
OrthoDB	EOG470TH4.
Gene expression databases
Genevestigator	P43144.
GermOnline	ENSRNOG00000002484. Rattus norvegicus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P43144.
ChEMBL	CHEMBL2585.
NextBio	613788.

Entry information

Entry name

ACHA9_RAT

Accession

Primary (citable) accession number: P43144
Secondary accession number(s): Q6PW49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 21, 2005
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents