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Protein

Neuronal acetylcholine receptor subunit alpha-9

Gene

Chrna9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.Curated6 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-9
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-9
Short name:
NACHR alpha-9
Gene namesi
Name:Chrna9
Synonyms:Acra9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi621534. Chrna9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 237212ExtracellularSequence analysisAdd
BLAST
Transmembranei238 – 26225HelicalSequence analysisAdd
BLAST
Transmembranei269 – 28719HelicalSequence analysisAdd
BLAST
Transmembranei302 – 32322HelicalSequence analysisAdd
BLAST
Topological domaini324 – 457134CytoplasmicSequence analysisAdd
BLAST
Transmembranei458 – 47619HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • cell junction Source: UniProtKB-KW
  • cytosol Source: Ensembl
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094110.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 479454Neuronal acetylcholine receptor subunit alpha-9PRO_0000000372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Disulfide bondi155 ↔ 169By similarity
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Disulfide bondi219 ↔ 220By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43144.

PTM databases

PhosphoSiteiP43144.

Expressioni

Tissue specificityi

Detected in the nasal epithelium, in the outer hair cells of the cochlea, in the pars tuberalis of the hypophysis, and in the developing muscle of the tongue. Also expressed in the neurons of dorsal root ganglia.3 Publications

Gene expression databases

GenevisibleiP43144. RN.

Interactioni

Subunit structurei

Can form homo- or hetero-oligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.Curated3 Publications

Protein-protein interaction databases

MINTiMINT-4996733.
STRINGi10116.ENSRNOP00000003382.

Chemistry

BindingDBiP43144.

Structurei

3D structure databases

ProteinModelPortaliP43144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP43144.
KOiK04810.
OMAiMGMDSGD.
OrthoDBiEOG091G0R20.
PhylomeDBiP43144.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPHSCLSF CWMYFAASGI RAVETANGKY AQKLFSDLFE DYSSALRPVE
60 70 80 90 100
DTDAVLNVTL QVTLSQIKDM DERNQILTAY LWIRQTWHDA YLTWDRDQYD
110 120 130 140 150
RLDSIRIPSD LVWRPDIVLY NKADDESSEP VNTNVVLRYD GLITWDSPAI
160 170 180 190 200
TKSSCVVDVT YFPFDSQQCN LTFGSWTYNG NQVDIFNALD SGDLSDFIED
210 220 230 240 250
VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY IVNLLIPCVL
260 270 280 290 300
ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG
310 320 330 340 350
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWAKVV ILKYMSRILF
360 370 380 390 400
VYDVGESCLS PRHSQEPEQV TKVYSKLPES NLKTSRNKDL SRKKEVRKLL
410 420 430 440 450
KNDLGYQGGI PQNTDSYCAR YEALTKNIEY IAKCLKDHKA TNSKGSEWKK
460 470
VAKVIDRFFM WIFFAMVFVM TVLIIARAD
Length:479
Mass (Da):54,526
Last modified:June 21, 2005 - v2
Checksum:iCBB3E15E9DF68837
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti425 – 4251T → A in AAA56720 (PubMed:7954834).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12336 mRNA. Translation: AAA56720.1.
AY574257 mRNA. Translation: AAS90353.1.
PIRiA55382.
RefSeqiNP_075219.1. NM_022930.1.
UniGeneiRn.54463.

Genome annotation databases

EnsembliENSRNOT00000003382; ENSRNOP00000003382; ENSRNOG00000002484.
GeneIDi65024.
KEGGirno:65024.
UCSCiRGD:621534. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12336 mRNA. Translation: AAA56720.1.
AY574257 mRNA. Translation: AAS90353.1.
PIRiA55382.
RefSeqiNP_075219.1. NM_022930.1.
UniGeneiRn.54463.

3D structure databases

ProteinModelPortaliP43144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996733.
STRINGi10116.ENSRNOP00000003382.

Chemistry

BindingDBiP43144.
ChEMBLiCHEMBL2094110.

PTM databases

PhosphoSiteiP43144.

Proteomic databases

PaxDbiP43144.

Protocols and materials databases

DNASUi65024.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003382; ENSRNOP00000003382; ENSRNOG00000002484.
GeneIDi65024.
KEGGirno:65024.
UCSCiRGD:621534. rat.

Organism-specific databases

CTDi55584.
RGDi621534. Chrna9.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP43144.
KOiK04810.
OMAiMGMDSGD.
OrthoDBiEOG091G0R20.
PhylomeDBiP43144.
TreeFamiTF315605.

Miscellaneous databases

PROiP43144.

Gene expression databases

GenevisibleiP43144. RN.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACHA9_RAT
AccessioniPrimary (citable) accession number: P43144
Secondary accession number(s): Q6PW49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 21, 2005
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.