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Protein

Neuronal acetylcholine receptor subunit alpha-9

Gene

Chrna9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding induces a conformation change that leads to the opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion.Curated6 Publications

Miscellaneous

The heterooligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei86Key residue important for potent inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA (AC P0C1D0)1 Publication1
Sitei146Key residue important for potent inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA (AC P0C1D0)1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalcium channel, Ion channel, Ligand-gated ion channel, Receptor
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Enzyme and pathway databases

ReactomeiR-RNO-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-9
Alternative name(s):
Nicotinic acetylcholine receptor subunit alpha-9
Short name:
NACHR alpha-9
Gene namesi
Name:Chrna9
Synonyms:Acra9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi621534. Chrna9.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 237ExtracellularSequence analysisAdd BLAST212
Transmembranei238 – 262HelicalSequence analysisAdd BLAST25
Transmembranei269 – 287HelicalSequence analysisAdd BLAST19
Transmembranei302 – 323HelicalSequence analysisAdd BLAST22
Topological domaini324 – 457CytoplasmicSequence analysisAdd BLAST134
Transmembranei458 – 476HelicalSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36S → N: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi44S → N: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptorby the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi54A → K: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptorby the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi86T → E: The CHRNA9-CHRNA10 receptor is 20-fold less potently inhibited by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi86T → I: The CHRNA9-CHRNA10 receptor is 1700-fold less potently inhibited by the alpha-conotoxin RgIA. 2 Publications1
Mutagenesisi101R → G: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi146D → L: Complete loss of inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi147S → A: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi166S → N: No significant change in potency of inhibition of the CHRNA9-CHRNA10 receptor by the alpha-conotoxin RgIA. 1 Publication1
Mutagenesisi176W → T: The CHRNA9-CHRNA10 receptor is 8.5-fold less potently inhibited by the alpha-conotoxin RgIA. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2585.
GuidetoPHARMACOLOGYi469.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000037226 – 479Neuronal acetylcholine receptor subunit alpha-9Add BLAST454

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi155 ↔ 169By similarity
Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi219 ↔ 220By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP43144.
PRIDEiP43144.

PTM databases

PhosphoSitePlusiP43144.

Expressioni

Tissue specificityi

Detected in the nasal epithelium, in the outer hair cells of the cochlea, in the pars tuberalis of the hypophysis, and in the developing muscle of the tongue. Also expressed in the neurons of dorsal root ganglia.3 Publications

Gene expression databases

BgeeiENSRNOG00000002484.
GenevisibleiP43144. RN.

Interactioni

Subunit structurei

Can form homo- or heterooligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 heterooligomers. Interacts with the alpha-conotoxin RgIA (PubMed:25740413).Curated4 Publications

Protein-protein interaction databases

MINTiMINT-4996733.
STRINGi10116.ENSRNOP00000003382.

Chemistry databases

BindingDBiP43144.

Structurei

3D structure databases

ProteinModelPortaliP43144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00790000122957.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP43144.
KOiK04810.
OMAiATKNVIM.
OrthoDBiEOG091G0R20.
PhylomeDBiP43144.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.58.390. 1 hit.
2.70.170.10. 1 hit.
InterProiView protein in InterPro
IPR006202. Neur_chan_lig-bd.
IPR036734. Neur_chan_lig-bd_sf.
IPR006201. Neur_channel.
IPR036719. Neuro-gated_channel_TM_sf.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiView protein in Pfam
PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiView protein in PROSITE
PS00236. NEUROTR_ION_CHANNEL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPHSCLSF CWMYFAASGI RAVETANGKY AQKLFSDLFE DYSSALRPVE
60 70 80 90 100
DTDAVLNVTL QVTLSQIKDM DERNQILTAY LWIRQTWHDA YLTWDRDQYD
110 120 130 140 150
RLDSIRIPSD LVWRPDIVLY NKADDESSEP VNTNVVLRYD GLITWDSPAI
160 170 180 190 200
TKSSCVVDVT YFPFDSQQCN LTFGSWTYNG NQVDIFNALD SGDLSDFIED
210 220 230 240 250
VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY IVNLLIPCVL
260 270 280 290 300
ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG
310 320 330 340 350
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWAKVV ILKYMSRILF
360 370 380 390 400
VYDVGESCLS PRHSQEPEQV TKVYSKLPES NLKTSRNKDL SRKKEVRKLL
410 420 430 440 450
KNDLGYQGGI PQNTDSYCAR YEALTKNIEY IAKCLKDHKA TNSKGSEWKK
460 470
VAKVIDRFFM WIFFAMVFVM TVLIIARAD
Length:479
Mass (Da):54,526
Last modified:June 21, 2005 - v2
Checksum:iCBB3E15E9DF68837
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti425T → A in AAA56720 (PubMed:7954834).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12336 mRNA. Translation: AAA56720.1.
AY574257 mRNA. Translation: AAS90353.1.
PIRiA55382.
RefSeqiNP_075219.1. NM_022930.1.
UniGeneiRn.54463.

Genome annotation databases

EnsembliENSRNOT00000003382; ENSRNOP00000003382; ENSRNOG00000002484.
GeneIDi65024.
KEGGirno:65024.
UCSCiRGD:621534. rat.

Similar proteinsi

Entry informationi

Entry nameiACHA9_RAT
AccessioniPrimary (citable) accession number: P43144
Secondary accession number(s): Q6PW49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 21, 2005
Last modified: October 25, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families