Neuronal acetylcholine receptor subunit alpha-9 precursor

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Reviewed, UniProtKB/Swiss-Prot P43144 (ACHA9_RAT)

Last modified November 24, 2009. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Neuronal acetylcholine receptor subunit alpha-9
Alternative name(s):
    Nicotinic acetylcholine receptor subunit alpha 9
      Short name=NACHR alpha 9

Gene names

Name:	Chrna9
Synonyms:	Acra9

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this leads to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. May also regulate keratinocyte adhesion. Ref.1 Ref.3 Ref.4 Ref.6 Ref.8
Subunit structure	Can form homo- or hetero-oligomeric channels in conjunction with CHRNA10. The native outer hair cell receptor may be composed of CHRNA9-CHRNA10 hetero-oligomers.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.
Tissue specificity	Detected in the nasal epithelium, in the outer hair cells of the cochlea, in the pars tuberalis of the hypophysis, and in the developing muscle of the tongue. Also expressed in the neurons of dorsal root ganglia. Ref.1 Ref.5 Ref.7
Miscellaneous	The hetero-oligomeric receptor composed of CHRNA9 and CHRNA10 has an atypical pharmacological profile, binding several non-nicotinic ligands including strychnine (a glycine receptor antagonist) and atropine (a muscarinic acetylcholine receptor antagonist).
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane
Ligand	Calcium
Molecular function	Calcium channel Ionic channel Receptor
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW nicotinic acetylcholine-gated receptor-channel complex Ref.7 Inferred from direct assay. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nicotinic acetylcholine-activated cation-selective channel activity Ref.1 Inferred from direct assay. Source: RGD receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

Potential

Chain

26 – 479

454

Neuronal acetylcholine receptor subunit alpha-9

PRO_0000000372

Regions

Topological domain

26 – 237

212

Extracellular Potential

Transmembrane

238 – 262

Potential

Transmembrane

269 – 287

Potential

Transmembrane

302 – 323

Potential

Topological domain

324 – 457

134

Cytoplasmic Potential

Transmembrane

458 – 476

Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

170

N-linked (GlcNAc...) Potential

Disulfide bond

155 ↔ 169

By similarity

Disulfide bond

219 ↔ 220

Associated with receptor activation By similarity

Experimental info

Sequence conflict

425

T → A in AAA56720. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

P43144-1 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: CBB3E15E9DF68837
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FASTA

479

54,526

        10         20         30         40         50         60 
MNRPHSCLSF CWMYFAASGI RAVETANGKY AQKLFSDLFE DYSSALRPVE DTDAVLNVTL 

        70         80         90        100        110        120 
QVTLSQIKDM DERNQILTAY LWIRQTWHDA YLTWDRDQYD RLDSIRIPSD LVWRPDIVLY 

       130        140        150        160        170        180 
NKADDESSEP VNTNVVLRYD GLITWDSPAI TKSSCVVDVT YFPFDSQQCN LTFGSWTYNG 

       190        200        210        220        230        240 
NQVDIFNALD SGDLSDFIED VEWEVHGMPA VKNVISYGCC SEPYPDVTFT LLLKRRSSFY 

       250        260        270        280        290        300 
IVNLLIPCVL ISFLAPLSFY LPAASGEKVS LGVTILLAMT VFQLMVAEIM PASENVPLIG 

       310        320        330        340        350        360 
KYYIATMALI TASTALTIMV MNIHFCGAEA RPVPHWAKVV ILKYMSRILF VYDVGESCLS 

       370        380        390        400        410        420 
PRHSQEPEQV TKVYSKLPES NLKTSRNKDL SRKKEVRKLL KNDLGYQGGI PQNTDSYCAR 

       430        440        450        460        470 
YEALTKNIEY IAKCLKDHKA TNSKGSEWKK VAKVIDRFFM WIFFAMVFVM TVLIIARAD

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References

[1]	"Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells." Elgoyhen A.B., Johnson D.S., Boulter J., Vetter D.E., Heinemann S.F. Cell 79:705-715(1994) [PubMed: 7954834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Olfactory epithelium.
[2]	Groot-Kormelink P.J. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"High calcium permeability and calcium block of the alpha9 nicotinic acetylcholine receptor." Katz E., Verbitsky M., Rothlin C.V., Vetter D.E., Heinemann S.F., Elgoyhen A.B. Hear. Res. 141:117-128(2000) [PubMed: 10713500] [Abstract] Cited for: FUNCTION.
[4]	"Mixed nicotinic-muscarinic properties of the alpha9 nicotinic cholinergic receptor." Verbitsky M., Rothlin C.V., Katz E., Elgoyhen A.B. Neuropharmacology 39:2515-2524(2000) [PubMed: 11044723] [Abstract] Cited for: FUNCTION.
[5]	"Alpha10: a determinant of nicotinic cholinergic receptor function in mammalian vestibular and cochlear mechanosensory hair cells." Elgoyhen A.B., Vetter D.E., Katz E., Rothlin C.V., Heinemann S.F., Boulter J. Proc. Natl. Acad. Sci. U.S.A. 98:3501-3506(2001) [PubMed: 11248107] [Abstract] Cited for: INTERACTION WITH CHRNA10, TISSUE SPECIFICITY.
[6]	"The alpha9alpha10 nicotinic acetylcholine receptor is permeable to and is modulated by divalent cations." Weisstaub N., Vetter D.E., Elgoyhen A.B., Katz E. Hear. Res. 167:122-135(2002) [PubMed: 12117536] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHRNA10.
[7]	"Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons." Lips K.S., Pfeil U., Kummer W. Neuroscience 115:1-5(2002) [PubMed: 12401316] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Pharmacological properties of alpha 9 alpha 10 nicotinic acetylcholine receptors revealed by heterologous expression of subunit chimeras." Baker E.R., Zwart R., Sher E., Millar N.S. Mol. Pharmacol. 65:453-460(2004) [PubMed: 14742688] [Abstract] Cited for: FUNCTION, INTERACTION WITH CHRNA10.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U12336 mRNA. Translation: AAA56720.1. AY574257 mRNA. Translation: AAS90353.1.
IPI	IPI00197478.
PIR	A55382.
RefSeq	NP_075219.1.
UniGene	Rn.54463
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P43144.
Genome annotation databases
Ensembl	ENSRNOT00000003382; ENSRNOP00000003382; ENSRNOG00000002484; Rattus norvegicus. [Genome view]
GeneID	65024.
KEGG	rno:65024.
UCSC	NM_022930. rat.
Organism-specific databases
CTD	65024.
RGD	621534. Chrna9.
Phylogenomic databases
HOVERGEN	P43144.
OMA	VYSKLPE
OrthoDB	EOG95F080
Gene expression databases
ArrayExpress	P43144.
Genevestigator	P43144.
GermOnline	ENSRNOG00000002484. Rattus norvegicus.
Family and domain databases
InterPro	IPR006029. Neu_channel_TM. IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt_N. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613788.

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Entry information

Entry name

ACHA9_RAT

Accession

Primary (citable) accession number: P43144
Secondary accession number(s): Q6PW49

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 21, 2005
Last modified:	November 24, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents