P43140 (ADA1A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1A adrenergic receptor

Alternative name(s):
Alpha-1A adrenoreceptor
Short name=Alpha-1A adrenoceptor
Alpha-1C adrenergic receptor

Gene names

Name:	Adra1a
Synonyms:	Adra1c

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Abundant in heart, brain, aorta, vena cava, vas deferens, submaxillary gland, lung, and kidney. Found at lower levels in prostate, parotid gland and skeletal muscle. Ref.3
Post-translational modification	Carboxyl-terminal Ser or Thr residues may be phosphorylated By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aging Inferred from direct assay. Source: RGD calcium ion transport into cytosol Inferred from mutant phenotype. Source: BHF-UCL negative regulation of Rho protein signal transduction Inferred from mutant phenotype. Source: MGI negative regulation of synaptic transmission, GABAergic Inferred from direct assay. Source: RGD positive regulation of ERK1 and ERK2 cascade Inferred from mutant phenotype. Source: BHF-UCL positive regulation of action potential Inferred from mutant phenotype. Source: RGD positive regulation of cardiac muscle contraction Inferred from mutant phenotype. Source: BHF-UCL positive regulation of protein kinase C signaling cascade Inferred from mutant phenotype. Source: BHF-UCL positive regulation of vasoconstriction Inferred from mutant phenotype. Source: BHF-UCL response to drug Inferred from mutant phenotype. Source: RGD response to hormone stimulus Inferred from mutant phenotype. Source: RGD response to stress Inferred from direct assay. Source: RGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha1-adrenergic receptor activity Inferred from direct assay Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Alpha-1A adrenergic receptor		PRO_0000069066
Regions
	Topological domain	1 – 27	27	Extracellular By similarity
	Transmembrane	28 – 51	24	Helical; Name=1; By similarity
	Topological domain	52 – 64	13	Cytoplasmic By similarity
	Transmembrane	65 – 88	24	Helical; Name=2; By similarity
	Topological domain	89 – 99	11	Extracellular By similarity
	Transmembrane	100 – 122	23	Helical; Name=3; By similarity
	Topological domain	123 – 143	21	Cytoplasmic By similarity
	Transmembrane	144 – 167	24	Helical; Name=4; By similarity
	Topological domain	168 – 181	14	Extracellular By similarity
	Transmembrane	182 – 205	24	Helical; Name=5; By similarity
	Topological domain	206 – 273	68	Cytoplasmic By similarity
	Transmembrane	274 – 297	24	Helical; Name=6; By similarity
	Topological domain	298 – 305	8	Extracellular By similarity
	Transmembrane	306 – 329	24	Helical; Name=7; By similarity
	Topological domain	330 – 466	137	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	215	1	Phosphoserine; by PKA Potential
	Lipidation	345	1	S-palmitoyl cysteine Potential
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 176		By similarity
Experimental info
	Sequence conflict	39	1	F → L in AAA52103. Ref.1
	Sequence conflict	67	1	N → G in AAA52103. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P43140 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4962D3A72E971A36	FASTA	466	51,598
	10 20 30 40 50 60 MVLLSENASE GSNCTHPPAP VNISKAILLG VILGGLIIFG VLGNILVILS VACHRHLHSV 70 80 90 100 110 120 THYYIVNLAV ADLLLTSTVL PFSAIFEILG YWAFGRVFCN IWAAVDVLCC TASIMGLCII 130 140 150 160 170 180 SIDRYIGVSY PLRYPTIVTQ RRGVRALLCV WVLSLVISIG PLFGWRQPAP EDETICQINE 190 200 210 220 230 240 EPGYVLFSAL GSFYVPLAII LVMYCRVYVV AKRESRGLKS GLKTDKSDSE QVTLRIHRKN 250 260 270 280 290 300 VPAEGGGVSS AKNKTHFSVR LLKFSREKKA AKTLGIVVGC FVLCWLPFFL VMPIGSFFPD 310 320 330 340 350 360 FKPSETVFKI VFWLGYLNSC INPIIYPCSS QEFKKAFQNV LRIQCLRRRQ SSKHALGYTL 370 380 390 400 410 420 HPPSQALEGQ HRDMVRIPVG SGETFYKISK TDGVCEWKFF SSMPQGSARI TVPKDQSACT 430 440 450 460 TARVRSKSFL QVCCCVGSSA PRPEENHQVP TIKIHTISLG ENGEEV

References

[1]	"Cloning of the rat alpha 1C-adrenergic receptor from cardiac myocytes. Alpha 1C, alpha 1B, and alpha 1D mRNAs are present in cardiac myocytes but not in cardiac fibroblasts." Stewart A.F., Rokosh D.G., Bailey B.A., Karns L.R., Chang K.C., Long C.S., Kariya K., Simpson P.C. Circ. Res. 75:796-802(1994) [PubMed: 7923624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Heart muscle.
[2]	"The rat homologue of the bovine alpha 1c-adrenergic receptor shows the pharmacological properties of the classical alpha 1A subtype." Laz T.M., Forray C., Smith K.E., Bard J.A., Vaysse P.J., Branchek T.A., Weinshank R.L. Mol. Pharmacol. 46:414-422(1994) [PubMed: 7935320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Distribution of alpha 1C-adrenergic receptor mRNA in adult rat tissues by RNase protection assay and comparison with alpha 1B and alpha 1D." Rokosh D.G., Bailey B.A., Stewart A.F., Karns L.R., Long C.S., Simpson P.C. Biochem. Biophys. Res. Commun. 200:1177-1184(1994) [PubMed: 8185565] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U13368 mRNA. Translation: AAA52103.1.
U07126 mRNA. Translation: AAA62866.1.

IPI

IPI00324966.

PIR

I57959.

RefSeq

NP_058887.2. NM_017191.2.

UniGene

Rn.9991.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F75	model	-	A	1-466	[»]

ProteinModelPortal

P43140.

ModBase

Search...

Protein-protein interaction databases

STRING

P43140.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P43140.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000012736; ENSRNOP00000012736; ENSRNOG00000009522.

GeneID

29412.

KEGG

rno:29412.

UCSC

NM_017191. rat.

Organism-specific databases

CTD

148.

RGD

2055. Adra1a.

Phylogenomic databases

eggNOG

roNOG12277.

HOVERGEN

HBG106962.

InParanoid

P43140.

OMA

RIHRKNV.

OrthoDB

EOG40VVPS.

PhylomeDB

P43140.

Gene expression databases

ArrayExpress

P43140.

Genevestigator

P43140.

GermOnline

ENSRNOG00000009522. Rattus norvegicus.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR001004. Adrene_rcpt_A1Cs.
IPR002233. Adrnrgc_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]

KO

K04135.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR01103. ADRENERGICR.
PR00557. ADRENRGCA1AR.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

609080.

Entry information

Entry name

ADA1A_RAT

Accession

Primary (citable) accession number: P43140

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 16, 2011
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program