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Protein

Alpha-1A adrenergic receptor

Gene

Adra1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes (By similarity).By similarity

GO - Molecular functioni

  • alpha1-adrenergic receptor activity Source: RGD
  • protein heterodimerization activity Source: UniProtKB

GO - Biological processi

  • activation of phospholipase C activity Source: RGD
  • adrenergic receptor signaling pathway Source: GOC
  • adult heart development Source: Ensembl
  • aging Source: RGD
  • calcium ion transport into cytosol Source: BHF-UCL
  • cell growth Source: Ensembl
  • micturition Source: RGD
  • negative regulation of heart rate involved in baroreceptor response to increased systemic arterial blood pressure Source: Ensembl
  • negative regulation of Rho protein signal transduction Source: MGI
  • norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure Source: Ensembl
  • organ growth Source: Ensembl
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: Ensembl
  • pilomotor reflex Source: Ensembl
  • positive regulation of action potential Source: RGD
  • positive regulation of cardiac muscle contraction Source: BHF-UCL
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  • positive regulation of heart rate Source: RGD
  • positive regulation of heart rate by epinephrine-norepinephrine Source: Ensembl
  • positive regulation of MAPK cascade Source: UniProtKB
  • positive regulation of protein kinase C signaling Source: BHF-UCL
  • positive regulation of smooth muscle contraction Source: RGD
  • positive regulation of synaptic transmission, GABAergic Source: RGD
  • positive regulation of systemic arterial blood pressure Source: RGD
  • positive regulation of the force of heart contraction by epinephrine-norepinephrine Source: Ensembl
  • positive regulation of vasoconstriction Source: BHF-UCL
  • response to drug Source: RGD
  • response to hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390696. Adrenoceptors.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1A adrenergic receptor
Alternative name(s):
Alpha-1A adrenoreceptor
Short name:
Alpha-1A adrenoceptor
Alpha-1C adrenergic receptor
Gene namesi
Name:Adra1a
Synonyms:Adra1c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi2055. Adra1a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727ExtracellularBy similarityAdd
BLAST
Transmembranei28 – 5124Helical; Name=1By similarityAdd
BLAST
Topological domaini52 – 6413CytoplasmicBy similarityAdd
BLAST
Transmembranei65 – 8824Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 9911ExtracellularBy similarityAdd
BLAST
Transmembranei100 – 12223Helical; Name=3By similarityAdd
BLAST
Topological domaini123 – 14321CytoplasmicBy similarityAdd
BLAST
Transmembranei144 – 16724Helical; Name=4By similarityAdd
BLAST
Topological domaini168 – 18114ExtracellularBy similarityAdd
BLAST
Transmembranei182 – 20524Helical; Name=5By similarityAdd
BLAST
Topological domaini206 – 27368CytoplasmicBy similarityAdd
BLAST
Transmembranei274 – 29724Helical; Name=6By similarityAdd
BLAST
Topological domaini298 – 3058ExtracellularBy similarity
Transmembranei306 – 32924Helical; Name=7By similarityAdd
BLAST
Topological domaini330 – 466137CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • nuclear membrane Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: RGD
  • T-tubule Source: RGD
  • Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1907610.
GuidetoPHARMACOLOGYi22.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Alpha-1A adrenergic receptorPRO_0000069066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence analysis
Glycosylationi13 – 131N-linked (GlcNAc...)Sequence analysis
Glycosylationi22 – 221N-linked (GlcNAc...)Sequence analysis
Disulfide bondi99 ↔ 176PROSITE-ProRule annotation
Modified residuei215 – 2151Phosphoserine; by PKASequence analysis
Lipidationi345 – 3451S-palmitoyl cysteineSequence analysis

Post-translational modificationi

C-terminal Ser or Thr residues may be phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP43140.

PTM databases

PhosphoSiteiP43140.

Expressioni

Tissue specificityi

Abundant in heart, brain, aorta, vena cava, vas deferens, submaxillary gland, lung, and kidney. Found at lower levels in prostate, parotid gland and skeletal muscle.1 Publication

Gene expression databases

GenevisibleiP43140. RN.

Interactioni

Subunit structurei

Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012736.

Chemistry

BindingDBiP43140.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F75model-A1-466[»]
ProteinModelPortaliP43140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi334 – 34916Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239244.
HOVERGENiHBG106962.
InParanoidiP43140.
KOiK04135.
OMAiTVPKDQS.
OrthoDBiEOG7H4DTB.
PhylomeDBiP43140.
TreeFamiTF331895.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001004. ADRA1A_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00557. ADRENRGCA1AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLSENASE GSNCTHPPAP VNISKAILLG VILGGLIIFG VLGNILVILS
60 70 80 90 100
VACHRHLHSV THYYIVNLAV ADLLLTSTVL PFSAIFEILG YWAFGRVFCN
110 120 130 140 150
IWAAVDVLCC TASIMGLCII SIDRYIGVSY PLRYPTIVTQ RRGVRALLCV
160 170 180 190 200
WVLSLVISIG PLFGWRQPAP EDETICQINE EPGYVLFSAL GSFYVPLAII
210 220 230 240 250
LVMYCRVYVV AKRESRGLKS GLKTDKSDSE QVTLRIHRKN VPAEGGGVSS
260 270 280 290 300
AKNKTHFSVR LLKFSREKKA AKTLGIVVGC FVLCWLPFFL VMPIGSFFPD
310 320 330 340 350
FKPSETVFKI VFWLGYLNSC INPIIYPCSS QEFKKAFQNV LRIQCLRRRQ
360 370 380 390 400
SSKHALGYTL HPPSQALEGQ HRDMVRIPVG SGETFYKISK TDGVCEWKFF
410 420 430 440 450
SSMPQGSARI TVPKDQSACT TARVRSKSFL QVCCCVGSSA PRPEENHQVP
460
TIKIHTISLG ENGEEV
Length:466
Mass (Da):51,598
Last modified:November 1, 1995 - v1
Checksum:i4962D3A72E971A36
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391F → L in AAA52103 (PubMed:7923624).Curated
Sequence conflicti67 – 671N → G in AAA52103 (PubMed:7923624).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13368 mRNA. Translation: AAA52103.1.
U07126 mRNA. Translation: AAA62866.1.
PIRiI57959.
RefSeqiNP_058887.2. NM_017191.2.
XP_006252169.1. XM_006252107.2.
XP_008768966.1. XM_008770744.1.
UniGeneiRn.9991.

Genome annotation databases

EnsembliENSRNOT00000012736; ENSRNOP00000012736; ENSRNOG00000009522.
GeneIDi29412.
KEGGirno:29412.
UCSCiRGD:2055. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13368 mRNA. Translation: AAA52103.1.
U07126 mRNA. Translation: AAA62866.1.
PIRiI57959.
RefSeqiNP_058887.2. NM_017191.2.
XP_006252169.1. XM_006252107.2.
XP_008768966.1. XM_008770744.1.
UniGeneiRn.9991.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2F75model-A1-466[»]
ProteinModelPortaliP43140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012736.

Chemistry

BindingDBiP43140.
ChEMBLiCHEMBL1907610.
GuidetoPHARMACOLOGYi22.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP43140.

Proteomic databases

PaxDbiP43140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012736; ENSRNOP00000012736; ENSRNOG00000009522.
GeneIDi29412.
KEGGirno:29412.
UCSCiRGD:2055. rat.

Organism-specific databases

CTDi148.
RGDi2055. Adra1a.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239244.
HOVERGENiHBG106962.
InParanoidiP43140.
KOiK04135.
OMAiTVPKDQS.
OrthoDBiEOG7H4DTB.
PhylomeDBiP43140.
TreeFamiTF331895.

Enzyme and pathway databases

ReactomeiR-RNO-390696. Adrenoceptors.
R-RNO-416476. G alpha (q) signalling events.
R-RNO-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiP43140.

Gene expression databases

GenevisibleiP43140. RN.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001004. ADRA1A_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00557. ADRENRGCA1AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the rat alpha 1C-adrenergic receptor from cardiac myocytes. Alpha 1C, alpha 1B, and alpha 1D mRNAs are present in cardiac myocytes but not in cardiac fibroblasts."
    Stewart A.F., Rokosh D.G., Bailey B.A., Karns L.R., Chang K.C., Long C.S., Kariya K., Simpson P.C.
    Circ. Res. 75:796-802(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart muscle.
  2. "The rat homologue of the bovine alpha 1c-adrenergic receptor shows the pharmacological properties of the classical alpha 1A subtype."
    Laz T.M., Forray C., Smith K.E., Bard J.A., Vaysse P.J., Branchek T.A., Weinshank R.L.
    Mol. Pharmacol. 46:414-422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Distribution of alpha 1C-adrenergic receptor mRNA in adult rat tissues by RNase protection assay and comparison with alpha 1B and alpha 1D."
    Rokosh D.G., Bailey B.A., Stewart A.F., Karns L.R., Long C.S., Simpson P.C.
    Biochem. Biophys. Res. Commun. 200:1177-1184(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiADA1A_RAT
AccessioniPrimary (citable) accession number: P43140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.