Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P43136

- NR2F6_MOUSE

UniProt

P43136 - NR2F6_MOUSE

Protein

Nuclear receptor subfamily 2 group F member 6

Gene

Nr2f6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4+ T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi54 – 12976Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri57 – 7721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri93 – 11725NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    2. protein binding Source: IntAct
    3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    5. steroid hormone receptor activity Source: InterPro
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. detection of temperature stimulus involved in sensory perception of pain Source: MGI
    2. entrainment of circadian clock by photoperiod Source: MGI
    3. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    4. neuron development Source: MGI

    Keywords - Molecular functioni

    Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 2 group F member 6
    Alternative name(s):
    COUP transcription factor 3
    Short name:
    COUP-TF3
    V-erbA-related protein 2
    Short name:
    EAR-2
    Gene namesi
    Name:Nr2f6
    Synonyms:Ear-2, Ear2, Erbal2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1352453. Nr2f6.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Reduction of neurons in the locus coerulus of the developing cortex. Defects in circadian behavior. Hyperreactive lymphocytes, late-onset immunopathology and hypersusceptibility to Th17-dependent experimental autoimmune encephalomyelitis.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Nuclear receptor subfamily 2 group F member 6PRO_0000053614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei41 – 411PhosphoserineBy similarity
    Modified residuei84 – 841PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP43136.
    PRIDEiP43136.

    PTM databases

    PhosphoSiteiP43136.

    Expressioni

    Developmental stagei

    Initially expressed at E8.5 in the developing rhombencephalon. At E11.5 expression in the CNS rapidly decreases and in newborn and adult expression is not detectable in the brain with the exceptions of Purkinje neurons and the choroid plexi.1 Publication

    Gene expression databases

    ArrayExpressiP43136.
    BgeeiP43136.
    CleanExiMM_NR2F6.
    GenevestigatoriP43136.

    Interactioni

    Subunit structurei

    Binds DNA as dimer; homodimer and heterodimer with NR2F2 and probably NR2F1. Interacts with THRB By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rasd1O356265EBI-4319956,EBI-4319979

    Protein-protein interaction databases

    BioGridi199494. 4 interactions.
    IntActiP43136. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP43136.
    SMRiP43136. Positions 55-384.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 390228Ligand-bindingBy similarityAdd
    BLAST
    Regioni314 – 39077Important for dimerizationBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri57 – 7721NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri93 – 11725NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG277317.
    GeneTreeiENSGT00720000108595.
    HOGENOMiHOG000260820.
    HOVERGENiHBG005606.
    InParanoidiQ922G8.
    KOiK08549.
    OMAiSDMEAGD.
    OrthoDBiEOG72RMZ5.
    TreeFamiTF352097.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR003068. COUP_TF.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR01282. COUPTNFACTOR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43136-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMVTGGWGD PGGDTNGVDK AGGSYPRATE DDSASPPGAT SDAEPGDEER    50
    PGLQVDCVVC GDKSSGKHYG VFTCEGCKSF FKRSIRRNLS YTCRSNRDCQ 100
    IDQHHRNQCQ YCRLKKCFRV GMRKEAVQRG RIPHALPGPA ACSPPGATGV 150
    EPFTGPPVSE LIAQLLRAEP YPAAGRFGGG GAVLGIDNVC ELAARLLFST 200
    VEWARHAPFF PELPAADQVA LLRLSWSELF VLNAAQAALP LHTAPLLAAA 250
    GLHAAPMAAE RAVAFMDQVR AFQEQVDKLG RLQVDAAEYG CLKAIALFTP 300
    DACGLSDPAH VESLQEKAQV ALTEYVRAQY PSQPQRFGRL LLRLPALRAV 350
    PASLISQLFF MRLVGKTPIE TLIRDMLLSG STFNWPYGSG 390
    Length:390
    Mass (Da):41,968
    Last modified:July 27, 2011 - v2
    Checksum:i4C30B25C6CAE5338
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101D → G in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti84 – 841S → T in CAA54097. (PubMed:7947324)Curated
    Sequence conflicti84 – 841S → T in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti152 – 1521P → H in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti164 – 1652QL → HV in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti196 – 1983LLF → AV in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti239 – 2413LPL → VAV in AAA37532. (PubMed:8194772)Curated
    Sequence conflicti357 – 3571Q → K in CAA54097. (PubMed:7947324)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76654 mRNA. Translation: CAA54097.1.
    L25674 mRNA. Translation: AAA37532.1.
    AK131941 mRNA. Translation: BAE20887.1.
    AK171552 mRNA. Translation: BAE42523.1.
    CH466569 Genomic DNA. Translation: EDL28911.1.
    BC008138 mRNA. Translation: AAH08138.1.
    CCDSiCCDS22392.1.
    PIRiI48734. S44285.
    I49640.
    RefSeqiNP_034280.2. NM_010150.2.
    UniGeneiMm.28989.
    Mm.440170.

    Genome annotation databases

    EnsembliENSMUST00000002466; ENSMUSP00000002466; ENSMUSG00000002393.
    GeneIDi13864.
    KEGGimmu:13864.
    UCSCiuc009mcx.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76654 mRNA. Translation: CAA54097.1 .
    L25674 mRNA. Translation: AAA37532.1 .
    AK131941 mRNA. Translation: BAE20887.1 .
    AK171552 mRNA. Translation: BAE42523.1 .
    CH466569 Genomic DNA. Translation: EDL28911.1 .
    BC008138 mRNA. Translation: AAH08138.1 .
    CCDSi CCDS22392.1.
    PIRi I48734. S44285.
    I49640.
    RefSeqi NP_034280.2. NM_010150.2.
    UniGenei Mm.28989.
    Mm.440170.

    3D structure databases

    ProteinModelPortali P43136.
    SMRi P43136. Positions 55-384.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199494. 4 interactions.
    IntActi P43136. 1 interaction.

    PTM databases

    PhosphoSitei P43136.

    Proteomic databases

    PaxDbi P43136.
    PRIDEi P43136.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002466 ; ENSMUSP00000002466 ; ENSMUSG00000002393 .
    GeneIDi 13864.
    KEGGi mmu:13864.
    UCSCi uc009mcx.1. mouse.

    Organism-specific databases

    CTDi 2063.
    MGIi MGI:1352453. Nr2f6.

    Phylogenomic databases

    eggNOGi NOG277317.
    GeneTreei ENSGT00720000108595.
    HOGENOMi HOG000260820.
    HOVERGENi HBG005606.
    InParanoidi Q922G8.
    KOi K08549.
    OMAi SDMEAGD.
    OrthoDBi EOG72RMZ5.
    TreeFami TF352097.

    Miscellaneous databases

    NextBioi 284756.
    PROi P43136.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43136.
    Bgeei P43136.
    CleanExi MM_NR2F6.
    Genevestigatori P43136.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR003068. COUP_TF.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR01282. COUPTNFACTOR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression during development of three murine members of the COUP family of nuclear orphan receptors."
      Jonk L.J.C., de Jonge M.E.J., Pals C.E.G.M., Wissink S., Vervaart J.M.A., Schoorlemmer J., Kruijer W.
      Mech. Dev. 47:81-97(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The sequence of a murine cDNA encoding Ear-2, a nuclear orphan receptor."
      Barnhart K.M., Mellon P.L.
      Gene 142:313-314(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pituitary.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    6. "Heterodimeric interactions between chicken ovalbumin upstream promoter-transcription factor family members ARP1 and ear2."
      Avram D., Ishmael J.E., Nevrivy D.J., Peterson V.J., Lee S.H., Dowell P., Leid M.
      J. Biol. Chem. 274:14331-14336(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, INTERACTION WITH NR2F2.
    7. "Friend of GATA 2 physically interacts with chicken ovalbumin upstream promoter-TF2 (COUP-TF2) and COUP-TF3 and represses COUP-TF2-dependent activation of the atrial natriuretic factor promoter."
      Huggins G.S., Bacani C.J., Boltax J., Aikawa R., Leiden J.M.
      J. Biol. Chem. 276:28029-28036(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZFPM2.
    8. "Identification of a nuclear orphan receptor (Ear2) as a negative regulator of renin gene transcription."
      Liu X., Huang X., Sigmund C.D.
      Circ. Res. 92:1033-1040(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION.
    9. "Abnormal development of the locus coeruleus in Ear2(Nr2f6)-deficient mice impairs the functionality of the forebrain clock and affects nociception."
      Warnecke M., Oster H., Revelli J.P., Alvarez-Bolado G., Eichele G.
      Genes Dev. 19:614-625(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    11. "The nuclear orphan receptor NR2F6 suppresses lymphocyte activation and T helper 17-dependent autoimmunity."
      Hermann-Kleiter N., Gruber T., Lutz-Nicoladoni C., Thuille N., Fresser F., Labi V., Schiefermeier N., Warnecke M., Huber L., Villunger A., Eichele G., Kaminski S., Baier G.
      Immunity 29:205-216(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiNR2F6_MOUSE
    AccessioniPrimary (citable) accession number: P43136
    Secondary accession number(s): Q61504, Q922G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3