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Protein

COUP transcription factor 2

Gene

Nr2f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi76 – 15176Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri79 – 9921NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri115 – 13925NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: UniProtKB
  3. protein homodimerization activity Source: MGI
  4. retinoic acid binding Source: UniProtKB
  5. sequence-specific DNA binding Source: MGI
  6. sequence-specific DNA binding transcription factor activity Source: MGI
  7. steroid hormone receptor activity Source: InterPro
  8. zinc ion binding Source: InterPro

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. blood vessel morphogenesis Source: MGI
  3. fertilization Source: MGI
  4. forebrain development Source: MGI
  5. intracellular receptor signaling pathway Source: MGI
  6. in utero embryonic development Source: MGI
  7. limb development Source: MGI
  8. lymph vessel development Source: BHF-UCL
  9. maternal placenta development Source: MGI
  10. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  11. negative regulation of endothelial cell migration Source: MGI
  12. negative regulation of endothelial cell proliferation Source: MGI
  13. negative regulation of transcription, DNA-templated Source: MGI
  14. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  15. neuron migration Source: MGI
  16. placenta blood vessel development Source: MGI
  17. positive regulation of transcription, DNA-templated Source: UniProtKB
  18. radial pattern formation Source: MGI
  19. regulation of transcription from RNA polymerase II promoter Source: MGI
  20. regulation of transcription involved in lymphatic endothelial cell fate commitment Source: MGI
  21. response to estradiol Source: Ensembl
  22. skeletal muscle tissue development Source: MGI
  23. trophoblast giant cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
COUP transcription factor 2
Short name:
COUP-TF2
Alternative name(s):
Apolipoprotein AI regulatory protein 1
Short name:
ARP-1
COUP transcription factor II
Short name:
COUP-TF II
Nuclear receptor subfamily 2 group F member 2
Gene namesi
Name:Nr2f2
Synonyms:Aporp1, Arp-1, Arp1, Tfcoup2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1352452. Nr2f2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi363 – 3675LLLRL → RSLRF: Abolishes interaction with ZFPM2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 414414COUP transcription factor 2PRO_0000053607Add
BLAST

Proteomic databases

MaxQBiP43135.
PaxDbiP43135.
PRIDEiP43135.

PTM databases

PhosphoSiteiP43135.

Expressioni

Gene expression databases

BgeeiP43135.
CleanExiMM_NR2F2.
ExpressionAtlasiP43135. baseline and differential.
GenevestigatoriP43135.

Interactioni

Subunit structurei

Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A (By similarity). Interacts with ZFPM2.By similarity1 Publication

Protein-protein interaction databases

BioGridi198166. 1 interaction.
STRINGi10090.ENSMUSP00000032768.

Structurei

3D structure databases

ProteinModelPortaliP43135.
SMRiP43135. Positions 75-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 414298Interaction with ZFPM2Add
BLAST
Regioni183 – 414232Ligand-bindingBy similarityAdd
BLAST
Regioni337 – 41478Important for dimerizationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi71 – 755Poly-Gln

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri79 – 9921NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri115 – 13925NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG327099.
HOGENOMiHOG000260820.
HOVERGENiHBG005606.
InParanoidiP43135.
KOiK08548.
OMAiGPPNNTQ.
OrthoDBiEOG72RMZ5.
PhylomeDBiP43135.
TreeFamiTF352097.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43135-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMVVSTWRD PQDEVPGSQG SQASQAPPVP GPPPGAPHTP QTPGQGGPAS
60 70 80 90 100
TPAQTAAGGQ GGPGGPGSDK QQQQQHIECV VCGDKSSGKH YGQFTCEGCK
110 120 130 140 150
SFFKRSVRRN LSYTCRANRN CPIDQHHRNQ CQYCRLKKCL KVGMRREAVQ
160 170 180 190 200
RGRMPPTQPT HGQFALTNGD PLNCHSYLSG YISLLLRAEP YPTSRFGSQC
210 220 230 240 250
MQPNNIMGIE NICELAARML FSAVEWARNI PFFPDLQITD QVALLRLTWS
260 270 280 290 300
ELFVLNAAQC SMPLHVAPLL AAAGLHASPM SADRVVAFMD HIRIFQEQVE
310 320 330 340 350
KLKALHVDSA EYSCLKAIVL FTSDACGLSD VAHVESLQEK SQCALEEYVR
360 370 380 390 400
SQYPNQPTRF GKLLLRLPSL RTVSSSVIEQ LFFVRLVGKT PIETLIRDML
410
LSGSSFNWPY MAIQ
Length:414
Mass (Da):45,571
Last modified:April 13, 2004 - v2
Checksum:iC24CB2E8C8A27E8C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361A → P in CAA54096. (PubMed:7947324)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76653 mRNA. Translation: CAA54096.1.
U07635 mRNA. Translation: AAA19854.1.
BC042484 mRNA. Translation: AAH42484.1.
CCDSiCCDS21359.1.
RefSeqiNP_033827.2. NM_009697.3.
UniGeneiMm.158143.

Genome annotation databases

EnsembliENSMUST00000032768; ENSMUSP00000032768; ENSMUSG00000030551.
GeneIDi11819.
KEGGimmu:11819.
UCSCiuc009hni.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76653 mRNA. Translation: CAA54096.1.
U07635 mRNA. Translation: AAA19854.1.
BC042484 mRNA. Translation: AAH42484.1.
CCDSiCCDS21359.1.
RefSeqiNP_033827.2. NM_009697.3.
UniGeneiMm.158143.

3D structure databases

ProteinModelPortaliP43135.
SMRiP43135. Positions 75-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198166. 1 interaction.
STRINGi10090.ENSMUSP00000032768.

Chemistry

BindingDBiP43135.

PTM databases

PhosphoSiteiP43135.

Proteomic databases

MaxQBiP43135.
PaxDbiP43135.
PRIDEiP43135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032768; ENSMUSP00000032768; ENSMUSG00000030551.
GeneIDi11819.
KEGGimmu:11819.
UCSCiuc009hni.1. mouse.

Organism-specific databases

CTDi7026.
MGIiMGI:1352452. Nr2f2.

Phylogenomic databases

eggNOGiNOG327099.
HOGENOMiHOG000260820.
HOVERGENiHBG005606.
InParanoidiP43135.
KOiK08548.
OMAiGPPNNTQ.
OrthoDBiEOG72RMZ5.
PhylomeDBiP43135.
TreeFamiTF352097.

Miscellaneous databases

NextBioi279711.
PROiP43135.
SOURCEiSearch...

Gene expression databases

BgeeiP43135.
CleanExiMM_NR2F2.
ExpressionAtlasiP43135. baseline and differential.
GenevestigatoriP43135.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression during development of three murine members of the COUP family of nuclear orphan receptors."
    Jonk L.J.C., de Jonge M.E.J., Pals C.E.G.M., Wissink S., Vervaart J.M.A., Schoorlemmer J., Kruijer W.
    Mech. Dev. 47:81-97(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Spatiotemporal expression patterns of chicken ovalbumin upstream promoter-transcription factors in the developing mouse central nervous system: evidence for a role in segmental patterning of the diencephalon."
    Qiu Y., Cooney A.J., Kuratani S., DeMayo F.J., Tsai S.Y., Tsai M.J.
    Proc. Natl. Acad. Sci. U.S.A. 91:4451-4455(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "Friend of GATA 2 physically interacts with chicken ovalbumin upstream promoter-TF2 (COUP-TF2) and COUP-TF3 and represses COUP-TF2-dependent activation of the atrial natriuretic factor promoter."
    Huggins G.S., Bacani C.J., Boltax J., Aikawa R., Leiden J.M.
    J. Biol. Chem. 276:28029-28036(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZFPM2, MUTAGENESIS OF 363-LEU--LEU-367.

Entry informationi

Entry nameiCOT2_MOUSE
AccessioniPrimary (citable) accession number: P43135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 13, 2004
Last modified: February 4, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.