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P43135 (COT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
COUP transcription factor 2

Short name=COUP-TF2
Alternative name(s):
Apolipoprotein AI regulatory protein 1
Short name=ARP-1
COUP transcription factor II
Short name=COUP-TF II
Nuclear receptor subfamily 2 group F member 2
Gene names
Name:Nr2f2
Synonyms:Aporp1, Arp-1, Arp1, Tfcoup2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length414 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A By similarity.

Subunit structure

Interacts with SQSTM1. Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A By similarity. Interacts with ZFPM2. Ref.4

Subcellular location

Nucleus.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Receptor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from mutant phenotype PubMed 15829524. Source: MGI

blood vessel morphogenesis

Inferred from mutant phenotype PubMed 15875024. Source: MGI

fertilization

Inferred from mutant phenotype PubMed 17404209. Source: MGI

forebrain development

Inferred from direct assay PubMed 15548577. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 17404209. Source: MGI

limb development

Inferred from mutant phenotype PubMed 15546392. Source: MGI

lymph vessel development

Inferred from expression pattern PubMed 19210544. Source: BHF-UCL

maternal placenta development

Inferred from mutant phenotype PubMed 17404209. Source: MGI

negative regulation of cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 7823919. Source: MGI

neuron migration

Inferred from direct assay PubMed 15548577. Source: MGI

placenta blood vessel development

Inferred from mutant phenotype PubMed 17404209. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

radial pattern formation

Inferred from mutant phenotype PubMed 15829524. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19796622. Source: MGI

regulation of transcription involved in lymphatic endothelial cell fate commitment

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

skeletal muscle tissue development

Inferred from mutant phenotype PubMed 15546392PubMed 16251273. Source: MGI

trophoblast giant cell differentiation

Inferred from mutant phenotype PubMed 17404209. Source: MGI

   Cellular_componentnucleus

Inferred from direct assay PubMed 15548577PubMed 19796622. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16912278. Source: MGI

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

retinoic acid binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding

Inferred from direct assay PubMed 7823919. Source: MGI

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 414414COUP transcription factor 2
PRO_0000053607

Regions

DNA binding76 – 15176Nuclear receptor
Zinc finger79 – 9921NR C4-type
Zinc finger115 – 13925NR C4-type
Region117 – 414298Interaction with ZFPM2
Region183 – 414232Ligand-binding By similarity
Region337 – 41478Important for dimerization
Compositional bias71 – 755Poly-Gln

Experimental info

Mutagenesis363 – 3675LLLRL → RSLRF: Abolishes interaction with ZFPM2. Ref.4
Sequence conflict361A → P in CAA54096. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43135 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: C24CB2E8C8A27E8C

FASTA41445,571
        10         20         30         40         50         60 
MAMVVSTWRD PQDEVPGSQG SQASQAPPVP GPPPGAPHTP QTPGQGGPAS TPAQTAAGGQ 

        70         80         90        100        110        120 
GGPGGPGSDK QQQQQHIECV VCGDKSSGKH YGQFTCEGCK SFFKRSVRRN LSYTCRANRN 

       130        140        150        160        170        180 
CPIDQHHRNQ CQYCRLKKCL KVGMRREAVQ RGRMPPTQPT HGQFALTNGD PLNCHSYLSG 

       190        200        210        220        230        240 
YISLLLRAEP YPTSRFGSQC MQPNNIMGIE NICELAARML FSAVEWARNI PFFPDLQITD 

       250        260        270        280        290        300 
QVALLRLTWS ELFVLNAAQC SMPLHVAPLL AAAGLHASPM SADRVVAFMD HIRIFQEQVE 

       310        320        330        340        350        360 
KLKALHVDSA EYSCLKAIVL FTSDACGLSD VAHVESLQEK SQCALEEYVR SQYPNQPTRF 

       370        380        390        400        410 
GKLLLRLPSL RTVSSSVIEQ LFFVRLVGKT PIETLIRDML LSGSSFNWPY MAIQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression during development of three murine members of the COUP family of nuclear orphan receptors."
Jonk L.J.C., de Jonge M.E.J., Pals C.E.G.M., Wissink S., Vervaart J.M.A., Schoorlemmer J., Kruijer W.
Mech. Dev. 47:81-97(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Spatiotemporal expression patterns of chicken ovalbumin upstream promoter-transcription factors in the developing mouse central nervous system: evidence for a role in segmental patterning of the diencephalon."
Qiu Y., Cooney A.J., Kuratani S., DeMayo F.J., Tsai S.Y., Tsai M.J.
Proc. Natl. Acad. Sci. U.S.A. 91:4451-4455(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[4]"Friend of GATA 2 physically interacts with chicken ovalbumin upstream promoter-TF2 (COUP-TF2) and COUP-TF3 and represses COUP-TF2-dependent activation of the atrial natriuretic factor promoter."
Huggins G.S., Bacani C.J., Boltax J., Aikawa R., Leiden J.M.
J. Biol. Chem. 276:28029-28036(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZFPM2, MUTAGENESIS OF 363-LEU--LEU-367.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76653 mRNA. Translation: CAA54096.1.
U07635 mRNA. Translation: AAA19854.1.
BC042484 mRNA. Translation: AAH42484.1.
CCDSCCDS21359.1.
RefSeqNP_033827.2. NM_009697.3.
UniGeneMm.158143.

3D structure databases

ProteinModelPortalP43135.
SMRP43135. Positions 75-407.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198166. 1 interaction.
STRING10090.ENSMUSP00000032768.

PTM databases

PhosphoSiteP43135.

Proteomic databases

PaxDbP43135.
PRIDEP43135.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032768; ENSMUSP00000032768; ENSMUSG00000030551.
GeneID11819.
KEGGmmu:11819.
UCSCuc009hni.1. mouse.

Organism-specific databases

CTD7026.
MGIMGI:1352452. Nr2f2.

Phylogenomic databases

eggNOGNOG327099.
HOGENOMHOG000260820.
HOVERGENHBG005606.
InParanoidP43135.
KOK08548.
OMAGPPNNTQ.
OrthoDBEOG72RMZ5.
PhylomeDBP43135.
TreeFamTF352097.

Gene expression databases

ArrayExpressP43135.
BgeeP43135.
CleanExMM_NR2F2.
GenevestigatorP43135.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279711.
PROP43135.
SOURCESearch...

Entry information

Entry nameCOT2_MOUSE
AccessionPrimary (citable) accession number: P43135
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot