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Protein

UDP-N-acetylglucosamine pyrophosphorylase

Gene

QRI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 5480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-N-acetylglucosamine pyrophosphorylase (QRI1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123UTPBy similarity1
Binding sitei194UTPBy similarity1
Binding sitei221UTP; via amide nitrogenBy similarity1
Binding sitei222SubstrateBy similarity1
Binding sitei252UTPBy similarity1
Binding sitei377UTPBy similarity1
Binding sitei409SubstrateBy similarity1

GO - Molecular functioni

  • UDP-N-acetylglucosamine diphosphorylase activity Source: SGD

GO - Biological processi

  • UDP-N-acetylglucosamine biosynthetic process Source: SGD

Keywordsi

Molecular functionNucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YDL103C-MONOMER
YEAST:YDL103C-MONOMER
BRENDAi2.7.7.23 984
ReactomeiR-SCE-446210 Synthesis of UDP-N-acetyl-glucosamine
SABIO-RKiP43123
UniPathwayiUPA00113; UER00533

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Gene namesi
Name:QRI1
Synonyms:UAP1
Ordered Locus Names:YDL103C
ORF Names:D2362
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL103C
SGDiS000002261 QRI1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi111G → A: Decrease of activity. 1
Mutagenesisi112G → A: Loss of activity. 1
Mutagenesisi114G → A: Decrease of activity. 1
Mutagenesisi115T → A: Decrease of activity. 1
Mutagenesisi116R → A: Loss of activity. 1
Mutagenesisi117L → A: Decrease of activity. 1
Mutagenesisi122P → A: Decrease of activity. 1
Mutagenesisi123K → A: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857731 – 477UDP-N-acetylglucosamine pyrophosphorylaseAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphoserineCombined sources1
Modified residuei461PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43123
PaxDbiP43123
PRIDEiP43123

PTM databases

iPTMnetiP43123

Interactioni

Protein-protein interaction databases

BioGridi31959, 408 interactors
DIPiDIP-2837N
IntActiP43123, 7 interactors
MINTiP43123
STRINGi4932.YDL103C

Structurei

3D structure databases

ProteinModelPortaliP43123
SMRiP43123
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 112UTP bindingBy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 112Substrate bindingBy similarity4
Motifi302 – 303Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010072
HOGENOMiHOG000186273
InParanoidiP43123
KOiK00972
OMAiMESTKEF
OrthoDBiEOG092C31ZS

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR029044 Nucleotide-diphossugar_trans
IPR002618 UDPGP_fam
PfamiView protein in Pfam
PF01704 UDPGP, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

P43123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTKQLFIE AGQSQLFHNW ESLSRKDQEE LLSNLEQISS KRSPAKLLED
60 70 80 90 100
CQNAIKFSLA NSSKDTGVEI SPLPPTSYES LIGNSKKENE YWRLGLEAIG
110 120 130 140 150
KGEVAVILMA GGQGTRLGSS QPKGCYDIGL PSKKSLFQIQ AEKLIRLQDM
160 170 180 190 200
VKDKKVEIPW YIMTSGPTRA ATEAYFQEHN YFGLNKEQIT FFNQGTLPAF
210 220 230 240 250
DLTGKHFLMK DPVNLSQSPD GNGGLYRAIK ENKLNEDFDR RGIKHVYMYC
260 270 280 290 300
VDNVLSKIAD PVFIGFAIKH GFELATKAVR KRDAHESVGL IATKNEKPCV
310 320 330 340 350
IEYSEISNEL AEAKDKDGLL KLRAGNIVNH YYLVDLLKRD LDQWCENMPY
360 370 380 390 400
HIAKKKIPAY DSVTGKYTKP TEPNGIKLEQ FIFDVFDTVP LNKFGCLEVD
410 420 430 440 450
RCKEFSPLKN GPGSKNDNPE TSRLAYLKLG TSWLEDAGAI VKDGVLVEVS
460 470
SKLSYAGENL SQFKGKVFDR SGIVLEK
Length:477
Mass (Da):53,476
Last modified:November 1, 1995 - v1
Checksum:i2D656E31C17805FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011272 Genomic DNA Translation: BAA31203.1
X79380 Genomic DNA Translation: CAA55927.1
X95644 Genomic DNA Translation: CAA64910.1
Z74151 Genomic DNA Translation: CAA98670.1
BK006938 Genomic DNA Translation: DAA11757.1
PIRiS50738
RefSeqiNP_010180.1, NM_001180162.1

Genome annotation databases

EnsemblFungiiYDL103C; YDL103C; YDL103C
GeneIDi851455
KEGGisce:YDL103C

Similar proteinsi

Entry informationi

Entry nameiUAP1_YEAST
AccessioniPrimary (citable) accession number: P43123
Secondary accession number(s): D6VRP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health