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Protein

UDP-N-acetylglucosamine pyrophosphorylase

Gene

QRI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-N-acetylglucosamine pyrophosphorylase (QRI1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231UTPBy similarity
Binding sitei194 – 1941UTPBy similarity
Binding sitei221 – 2211UTP; via amide nitrogenBy similarity
Binding sitei222 – 2221SubstrateBy similarity
Binding sitei252 – 2521UTPBy similarity
Binding sitei377 – 3771UTPBy similarity
Binding sitei409 – 4091SubstrateBy similarity

GO - Molecular functioni

  • UDP-N-acetylglucosamine diphosphorylase activity Source: SGD

GO - Biological processi

  • UDP-N-acetylglucosamine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13180.
YEAST:YDL103C-MONOMER.
BRENDAi2.7.7.23. 984.
ReactomeiR-SCE-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP43123.
UniPathwayiUPA00113; UER00533.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Gene namesi
Name:QRI1
Synonyms:UAP1
Ordered Locus Names:YDL103C
ORF Names:D2362
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL103C.
SGDiS000002261. QRI1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi111 – 1111G → A: Decrease of activity.
Mutagenesisi112 – 1121G → A: Loss of activity.
Mutagenesisi114 – 1141G → A: Decrease of activity.
Mutagenesisi115 – 1151T → A: Decrease of activity.
Mutagenesisi116 – 1161R → A: Loss of activity.
Mutagenesisi117 – 1171L → A: Decrease of activity.
Mutagenesisi122 – 1221P → A: Decrease of activity.
Mutagenesisi123 – 1231K → A: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477UDP-N-acetylglucosamine pyrophosphorylasePRO_0000185773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181PhosphoserineCombined sources
Modified residuei461 – 4611PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP43123.

PTM databases

iPTMnetiP43123.

Interactioni

Protein-protein interaction databases

BioGridi31959. 64 interactions.
DIPiDIP-2837N.
IntActiP43123. 1 interaction.
MINTiMINT-1594338.

Structurei

3D structure databases

ProteinModelPortaliP43123.
SMRiP43123. Positions 8-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 1124UTP bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi109 – 1124Substrate bindingBy similarity
Motifi302 – 3032Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010072.
HOGENOMiHOG000186273.
InParanoidiP43123.
KOiK00972.
OMAiYAGENLV.
OrthoDBiEOG092C31ZS.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P43123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTKQLFIE AGQSQLFHNW ESLSRKDQEE LLSNLEQISS KRSPAKLLED
60 70 80 90 100
CQNAIKFSLA NSSKDTGVEI SPLPPTSYES LIGNSKKENE YWRLGLEAIG
110 120 130 140 150
KGEVAVILMA GGQGTRLGSS QPKGCYDIGL PSKKSLFQIQ AEKLIRLQDM
160 170 180 190 200
VKDKKVEIPW YIMTSGPTRA ATEAYFQEHN YFGLNKEQIT FFNQGTLPAF
210 220 230 240 250
DLTGKHFLMK DPVNLSQSPD GNGGLYRAIK ENKLNEDFDR RGIKHVYMYC
260 270 280 290 300
VDNVLSKIAD PVFIGFAIKH GFELATKAVR KRDAHESVGL IATKNEKPCV
310 320 330 340 350
IEYSEISNEL AEAKDKDGLL KLRAGNIVNH YYLVDLLKRD LDQWCENMPY
360 370 380 390 400
HIAKKKIPAY DSVTGKYTKP TEPNGIKLEQ FIFDVFDTVP LNKFGCLEVD
410 420 430 440 450
RCKEFSPLKN GPGSKNDNPE TSRLAYLKLG TSWLEDAGAI VKDGVLVEVS
460 470
SKLSYAGENL SQFKGKVFDR SGIVLEK
Length:477
Mass (Da):53,476
Last modified:November 1, 1995 - v1
Checksum:i2D656E31C17805FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011272 Genomic DNA. Translation: BAA31203.1.
X79380 Genomic DNA. Translation: CAA55927.1.
X95644 Genomic DNA. Translation: CAA64910.1.
Z74151 Genomic DNA. Translation: CAA98670.1.
BK006938 Genomic DNA. Translation: DAA11757.1.
PIRiS50738.
RefSeqiNP_010180.1. NM_001180162.1.

Genome annotation databases

EnsemblFungiiYDL103C; YDL103C; YDL103C.
GeneIDi851455.
KEGGisce:YDL103C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011272 Genomic DNA. Translation: BAA31203.1.
X79380 Genomic DNA. Translation: CAA55927.1.
X95644 Genomic DNA. Translation: CAA64910.1.
Z74151 Genomic DNA. Translation: CAA98670.1.
BK006938 Genomic DNA. Translation: DAA11757.1.
PIRiS50738.
RefSeqiNP_010180.1. NM_001180162.1.

3D structure databases

ProteinModelPortaliP43123.
SMRiP43123. Positions 8-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31959. 64 interactions.
DIPiDIP-2837N.
IntActiP43123. 1 interaction.
MINTiMINT-1594338.

PTM databases

iPTMnetiP43123.

Proteomic databases

MaxQBiP43123.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL103C; YDL103C; YDL103C.
GeneIDi851455.
KEGGisce:YDL103C.

Organism-specific databases

EuPathDBiFungiDB:YDL103C.
SGDiS000002261. QRI1.

Phylogenomic databases

GeneTreeiENSGT00390000010072.
HOGENOMiHOG000186273.
InParanoidiP43123.
KOiK00972.
OMAiYAGENLV.
OrthoDBiEOG092C31ZS.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00533.
BioCyciMetaCyc:MONOMER-13180.
YEAST:YDL103C-MONOMER.
BRENDAi2.7.7.23. 984.
ReactomeiR-SCE-446210. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP43123.

Miscellaneous databases

PROiP43123.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUAP1_YEAST
AccessioniPrimary (citable) accession number: P43123
Secondary accession number(s): D6VRP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.