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P43122 (QRI7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein QRI7, mitochondrial
Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein QRI7
Gene names
Name:QRI7
Ordered Locus Names:YDL104C
ORF Names:D2366
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Involved in mitochondrial genome maintenance. Ref.9 Ref.10

Subcellular location

Mitochondrion Ref.5 Ref.7.

Disruption phenotype

Loss of QRI7 leads to the formation of mitochondria with abnormal morphology and no DNA. Ref.8

Miscellaneous

Present with 1400 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentMitochondrion
   DomainTransit peptide
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processmitochondrial tRNA threonylcarbamoyladenosine modification

Inferred from mutant phenotype Ref.9. Source: SGD

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentmitochondrion

Inferred from direct assay Ref.5Ref.7. Source: SGD

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 407Probable tRNA threonylcarbamoyladenosine biosynthesis protein QRI7, mitochondrialPRO_0000096988

Sequences

Sequence LengthMass (Da)Tools
P43122 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 328A21F239D6DB25

FASTA40745,543
        10         20         30         40         50         60 
MISIKGTGRF LLDNYRIWQR RAFNRPIQLR KGYKVLAIET SCDDTCVSVL DRFSKSAAPN 

        70         80         90        100        110        120 
VLANLKDTLD SIDEGGIIPT KAHIHHQARI GPLTERALIE SNAREGIDLI CVTRGPGMPG 

       130        140        150        160        170        180 
SLSGGLDFAK GLAVAWNKPL IGVHHMLGHL LIPRMGTNGK VPQFPFVSLL VSGGHTTFVL 

       190        200        210        220        230        240 
SRAIDDHEIL CDTIDIAVGD SLDKCGRELG FKGTMIAREM EKFINQDIND QDFALKLEMP 

       250        260        270        280        290        300 
SPLKNSASKR NMLSFSFSAF ITALRTNLTK LGKTEIQELP EREIRSIAYQ VQESVFDHII 

       310        320        330        340        350        360 
NKLKHVLKSQ PEKFKNVREF VCSGGVSSNQ RLRTKLETEL GTLNSTSFFN FYYPPMDLCS 

       370        380        390        400 
DNSIMIGWAG IEIWESLRLV SDLDICPIRQ WPLNDLLSVD GWRTDQL

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the PHO2-POL3 (CDC2) region of chromosome IV of Saccharomyces cerevisiae."
Simon M., Benit P., Vassal A., Dubois C., Faye G.
Yeast 10:1653-1656(1994) [PubMed: 7725801] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 20.3 kb DNA fragment from the left arm of Saccharomyces cerevisiae chromosome IV contains the KIN28, MSS2, PHO2, POL3 and DUN1 genes, and six new open reading frames."
Saiz J.E., Buitrago M.J., Garcia R., Revuelta J.L., del Rey F.
Yeast 12:1077-1084(1996) [PubMed: 8896274] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]"Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance."
Oberto J., Breuil N., Hecker A., Farina F., Brochier-Armanet C., Culetto E., Forterre P.
Nucleic Acids Res. 37:5343-5352(2009) [PubMed: 19578062] [Abstract]
Cited for: FUNCTION IN MITOCHONDRIAL GENOME MAINTENANCE, DISRUPTION PHENOTYPE.
[9]"The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A."
Srinivasan M., Mehta P., Yu Y., Prugar E., Koonin E.V., Karzai A.W., Sternglanz R.
EMBO J. 30:873-881(2011) [PubMed: 21183954] [Abstract]
Cited for: FUNCTION IN T(6)A37 FORMATION.
[10]"A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification."
El Yacoubi B., Hatin I., Deutsch C., Kahveci T., Rousset J.P., Iwata-Reuyl D., Murzin A.G., de Crecy-Lagard V.
EMBO J. 30:882-893(2011) [PubMed: 21285948] [Abstract]
Cited for: FUNCTION IN T(6)A37 FORMATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X79380 Genomic DNA. Translation: CAA55926.1.
X95644 Genomic DNA. Translation: CAA64909.1.
Z74152 Genomic DNA. Translation: CAA98671.1.
BK006938 Genomic DNA. Translation: DAA11756.1.
PIRS50740.
RefSeqNP_010179.1. NM_001180163.1.

3D structure databases

ProteinModelPortalP43122.
SMRP43122. Positions 31-403.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5028N.
MINTMINT-543895.
STRINGP43122.

Protein family/group databases

MEROPSM22.005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL104C; YDL104C; YDL104C.
GeneID851454.
KEGGsce:YDL104C.
NMPDRfig|4932.3.peg.917.

Organism-specific databases

CYGDYDL104c.
SGDS000002262. QRI7.

Phylogenomic databases

eggNOGfuNOG05377.
GeneTreeEFGT00050000003924.
HOGENOMHBG304663.
OMAKDCNFSY.
OrthoDBEOG4F4WKN.

Gene expression databases

ArrayExpressP43122.
GenevestigatorP43122.
GermOnlineYDL104C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000905. Peptidase_M22.
IPR017860. Peptidase_M22_CS.
IPR017861. Peptidase_M22_subgr.
[Graphical view]
KOK01409.
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSPR00789. OSIALOPTASE.
TIGRFAMsTIGR00329. Gcp_kae1. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968720.

Entry information

Entry nameQRI7_YEAST
AccessionPrimary (citable) accession number: P43122
Secondary accession number(s): D6VRP6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents