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Protein

Hematopoietically-expressed homeobox protein Hhex

Gene

Hhex

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Recognizes the DNA sequence 5'-ATTAA-3' (By similarity). Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi138 – 19760HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: UniProtKB
  • B cell differentiation Source: BHF-UCL
  • cell proliferation Source: MGI
  • common bile duct development Source: MGI
  • DNA conformation change Source: MGI
  • embryonic heart tube development Source: MGI
  • embryonic organ development Source: MGI
  • endoderm development Source: MGI
  • forebrain development Source: MGI
  • forebrain morphogenesis Source: MGI
  • gall bladder development Source: MGI
  • hemopoiesis Source: MGI
  • hepatic duct development Source: MGI
  • hepatoblast differentiation Source: MGI
  • hepatocyte differentiation Source: MGI
  • interkinetic nuclear migration Source: MGI
  • in utero embryonic development Source: MGI
  • liver development Source: MGI
  • morphogenesis of an epithelium Source: MGI
  • mRNA export from nucleus Source: MGI
  • multicellular organism growth Source: MGI
  • myeloid leukocyte differentiation Source: MGI
  • negative regulation of angiogenesis Source: BHF-UCL
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
  • Notch signaling pathway Source: MGI
  • organ morphogenesis Source: MGI
  • pancreas development Source: MGI
  • poly(A)+ mRNA export from nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of Wnt signaling pathway Source: UniProtKB
  • primary lung bud formation Source: MGI
  • primitive streak formation Source: MGI
  • protein localization to nucleus Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to peptide hormone Source: Ensembl
  • response to wounding Source: Ensembl
  • signal transduction Source: MGI
  • thyroid gland development Source: MGI
  • tissue morphogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vasculogenesis Source: MGI
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hematopoietically-expressed homeobox protein Hhex
Short name:
Homeobox protein HEX
Short name:
mHex
Alternative name(s):
Homeobox protein PRH
Gene namesi
Name:Hhex
Synonyms:Prh, Prhx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96086. Hhex.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
  • protein-DNA complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Hematopoietically-expressed homeobox protein HhexPRO_0000049075Add
BLAST

Proteomic databases

MaxQBiP43120.
PRIDEiP43120.

PTM databases

PhosphoSiteiP43120.

Expressioni

Developmental stagei

Expressed during hematopoiesis.

Gene expression databases

BgeeiP43120.
CleanExiMM_HHEX.
ExpressionAtlasiP43120. baseline and differential.
GenevisibleiP43120. MM.

Interactioni

Protein-protein interaction databases

BioGridi200297. 7 interactions.
MINTiMINT-209977.
STRINGi10090.ENSMUSP00000025944.

Structurei

3D structure databases

ProteinModelPortaliP43120.
SMRiP43120. Positions 142-195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 134134Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG252905.
GeneTreeiENSGT00790000122955.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiP43120.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG7M0NS8.
PhylomeDBiP43120.
TreeFamiTF325047.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFPHPGPAA APAVGVPLYA PTPLLQPAHP TPFYIDDILG RGPAAPTPTP
60 70 80 90 100
TLPSPNSSFT SLVSSYRTPV YEPTPVHPAF SHHPAAALAA AYGPSGFGGP
110 120 130 140 150
LYPFPRTVND YTHALLRHDP LGKPLLWSPF LQRPLHKRKG GQVRFSNDQT
160 170 180 190 200
VELEKKFETQ KYLSPPERKR LAKMLQLSER QVKTWFQNRR AKWRRLKQEN
210 220 230 240 250
PQSNKKDALD SLDTSCEQGQ DLPSEQNKGA SLDRSQCSPS PASQEDPDSE
260 270
ISEDSDQEVD IEGDKGYFNA G
Length:271
Mass (Da):29,987
Last modified:November 1, 1995 - v1
Checksum:i376765557A71C962
GO

Sequence cautioni

The sequence BAB29163.1 differs from that shown. Reason: Frameshift at position 11. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101A → L in BAB29163 (PubMed:16141072).Curated
Sequence conflicti33 – 331F → L in BAA76714 (PubMed:10101294).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21524 Genomic DNA. Translation: CAA79729.1.
AB017132 Genomic DNA. Translation: BAA76714.1.
AF132550 Genomic DNA. Translation: AAF04349.1.
AK014111 mRNA. Translation: BAB29163.1. Frameshift.
AK033664 mRNA. Translation: BAC28416.1.
BC057986 mRNA. Translation: AAH57986.1.
CCDSiCCDS29778.1.
PIRiS30230.
RefSeqiNP_032271.1. NM_008245.3.
UniGeneiMm.33896.

Genome annotation databases

EnsembliENSMUST00000025944; ENSMUSP00000025944; ENSMUSG00000024986.
GeneIDi15242.
KEGGimmu:15242.
UCSCiuc008hio.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21524 Genomic DNA. Translation: CAA79729.1.
AB017132 Genomic DNA. Translation: BAA76714.1.
AF132550 Genomic DNA. Translation: AAF04349.1.
AK014111 mRNA. Translation: BAB29163.1. Frameshift.
AK033664 mRNA. Translation: BAC28416.1.
BC057986 mRNA. Translation: AAH57986.1.
CCDSiCCDS29778.1.
PIRiS30230.
RefSeqiNP_032271.1. NM_008245.3.
UniGeneiMm.33896.

3D structure databases

ProteinModelPortaliP43120.
SMRiP43120. Positions 142-195.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200297. 7 interactions.
MINTiMINT-209977.
STRINGi10090.ENSMUSP00000025944.

PTM databases

PhosphoSiteiP43120.

Proteomic databases

MaxQBiP43120.
PRIDEiP43120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025944; ENSMUSP00000025944; ENSMUSG00000024986.
GeneIDi15242.
KEGGimmu:15242.
UCSCiuc008hio.1. mouse.

Organism-specific databases

CTDi3087.
MGIiMGI:96086. Hhex.

Phylogenomic databases

eggNOGiNOG252905.
GeneTreeiENSGT00790000122955.
HOGENOMiHOG000049110.
HOVERGENiHBG055460.
InParanoidiP43120.
KOiK08024.
OMAiDNSCDQR.
OrthoDBiEOG7M0NS8.
PhylomeDBiP43120.
TreeFamiTF325047.

Miscellaneous databases

NextBioi287843.
PROiP43120.
SOURCEiSearch...

Gene expression databases

BgeeiP43120.
CleanExiMM_HHEX.
ExpressionAtlasiP43120. baseline and differential.
GenevisibleiP43120. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human."
    Bedford F.K., Ashworth A., Enver T., Wiedemann L.M.
    Nucleic Acids Res. 21:1245-1249(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  2. "Genomic organization and promoter analysis of a mouse homeobox gene, Hex."
    Myint Z., Inazu T., Tanaka T., Yamada K., Keng V.W., Inoue Y., Kuriyama M., Noguchi T.
    J. Biochem. 125:795-802(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "Genomic structure, cDNA mapping, and chromosomal localization of the mouse homeobox gene, Hex."
    Ghosh B., Jacobs H.C., Wiedemann L.M., Brown A., Bedford F.K., Nimmakayalu M.A., Ward D.C., Bogue C.W.
    Mamm. Genome 10:1023-1025(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum and Head.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  6. "Hex is a transcriptional repressor that contributes to anterior identity and suppresses Spemann organiser function."
    Brickman J.M., Jones C.M., Clements M., Smith J.C., Beddington R.S.P.
    Development 127:2303-2315(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Hex acts with beta-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal."
    Zamparini A.L., Watts T., Gardner C.E., Tomlinson S.R., Johnston G.I., Brickman J.M.
    Development 133:3709-3722(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHHEX_MOUSE
AccessioniPrimary (citable) accession number: P43120
Secondary accession number(s): Q544N6, Q9CRV1, Q9R1X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.