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P43120 (HHEX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hematopoietically-expressed homeobox protein Hhex

Short name=Homeobox protein HEX
Short name=mHex
Alternative name(s):
Homeobox protein PRH
Gene names
Name:Hhex
Synonyms:Prh, Prhx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes the DNA sequence 5'-ATTAA-3' By similarity. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. Ref.6 Ref.7

Subcellular location

Nucleus Probable.

Developmental stage

Expressed during hematopoiesis.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Sequence caution

The sequence BAB29163.1 differs from that shown. Reason: Frameshift at position 11.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
Wnt signaling pathway
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
Repressor
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell differentiation

Inferred from mutant phenotype PubMed 12522149. Source: BHF-UCL

DNA conformation change

Inferred from electronic annotation. Source: Ensembl

Wnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

anterior/posterior pattern specification

Inferred from mutant phenotype Ref.7. Source: UniProtKB

common bile duct development

Inferred from mutant phenotype PubMed 17580084. Source: MGI

embryonic heart tube development

Inferred from mutant phenotype PubMed 15459110. Source: MGI

embryonic organ development

Inferred from mutant phenotype PubMed 16364283. Source: MGI

endoderm development

Inferred from mutant phenotype PubMed 16364283. Source: MGI

forebrain development

Inferred from mutant phenotype PubMed 10804184PubMed 11291863PubMed 15459110. Source: MGI

forebrain morphogenesis

Inferred from mutant phenotype PubMed 21445260. Source: MGI

gall bladder development

Inferred from mutant phenotype PubMed 17580084. Source: MGI

hemopoiesis

Inferred from mutant phenotype PubMed 12791650PubMed 15728128. Source: MGI

hepatic duct development

Inferred from mutant phenotype PubMed 17580084. Source: MGI

hepatoblast differentiation

Inferred from mutant phenotype PubMed 17580084. Source: MGI

hepatocyte differentiation

Inferred from mutant phenotype PubMed 11027604. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 21445260. Source: MGI

interkinetic nuclear migration

Inferred from mutant phenotype PubMed 16364283. Source: MGI

liver development

Inferred from mutant phenotype PubMed 10804184PubMed 11027604PubMed 15459110PubMed 16364283. Source: MGI

morphogenesis of an epithelium

Inferred from mutant phenotype PubMed 17580084. Source: MGI

multicellular organism growth

Inferred from mutant phenotype PubMed 21445260. Source: MGI

myeloid leukocyte differentiation

Inferred from mutant phenotype PubMed 11027604. Source: MGI

negative regulation of angiogenesis

Inferred from direct assay PubMed 12588764. Source: BHF-UCL

negative regulation of cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.6. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.6. Source: UniProtKB

negative regulation of vascular endothelial growth factor receptor signaling pathway

Inferred from direct assay PubMed 12588764. Source: BHF-UCL

organ morphogenesis

Inferred from mutant phenotype PubMed 14736744. Source: MGI

pancreas development

Inferred from mutant phenotype PubMed 16364283. Source: MGI

poly(A)+ mRNA export from nucleus

Inferred from electronic annotation. Source: Ensembl

positive regulation of Wnt signaling pathway

Inferred from mutant phenotype Ref.7. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

primary lung bud formation

Inferred from mutant phenotype PubMed 16364283. Source: MGI

primitive streak formation

Inferred from mutant phenotype PubMed 21445260. Source: MGI

protein localization to nucleus

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from mutant phenotype PubMed 14736744. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 21445260. Source: MGI

response to peptide hormone

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from electronic annotation. Source: Ensembl

signal transduction

Inferred from mutant phenotype PubMed 21445260. Source: MGI

thyroid gland development

Inferred from mutant phenotype PubMed 10804184PubMed 15581879. Source: MGI

tissue morphogenesis

Inferred from mutant phenotype PubMed 21445260. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

vasculogenesis

Inferred from mutant phenotype PubMed 12791650PubMed 15728128. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 16854221. Source: MGI

nucleus

Inferred from direct assay PubMed 11044484PubMed 16854221. Source: MGI

protein-DNA complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionDNA binding, bending

Inferred from electronic annotation. Source: Ensembl

HMG box domain binding

Inferred from physical interaction PubMed 16582099. Source: UniProtKB

chromatin binding

Inferred from direct assay PubMed 16764824. Source: MGI

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 11139473. Source: BHF-UCL

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271Hematopoietically-expressed homeobox protein Hhex
PRO_0000049075

Regions

DNA binding138 – 19760Homeobox
Compositional bias1 – 134134Pro-rich

Experimental info

Sequence conflict101A → L in BAB29163. Ref.4
Sequence conflict331F → L in BAA76714. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P43120 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 376765557A71C962

FASTA27129,987
        10         20         30         40         50         60 
MQFPHPGPAA APAVGVPLYA PTPLLQPAHP TPFYIDDILG RGPAAPTPTP TLPSPNSSFT 

        70         80         90        100        110        120 
SLVSSYRTPV YEPTPVHPAF SHHPAAALAA AYGPSGFGGP LYPFPRTVND YTHALLRHDP 

       130        140        150        160        170        180 
LGKPLLWSPF LQRPLHKRKG GQVRFSNDQT VELEKKFETQ KYLSPPERKR LAKMLQLSER 

       190        200        210        220        230        240 
QVKTWFQNRR AKWRRLKQEN PQSNKKDALD SLDTSCEQGQ DLPSEQNKGA SLDRSQCSPS 

       250        260        270 
PASQEDPDSE ISEDSDQEVD IEGDKGYFNA G 

« Hide

References

« Hide 'large scale' references
[1]"HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human."
Bedford F.K., Ashworth A., Enver T., Wiedemann L.M.
Nucleic Acids Res. 21:1245-1249(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: C57BL/6J.
[2]"Genomic organization and promoter analysis of a mouse homeobox gene, Hex."
Myint Z., Inazu T., Tanaka T., Yamada K., Keng V.W., Inoue Y., Kuriyama M., Noguchi T.
J. Biochem. 125:795-802(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[3]"Genomic structure, cDNA mapping, and chromosomal localization of the mouse homeobox gene, Hex."
Ghosh B., Jacobs H.C., Wiedemann L.M., Brown A., Bedford F.K., Nimmakayalu M.A., Ward D.C., Bogue C.W.
Mamm. Genome 10:1023-1025(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cecum and Head.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[6]"Hex is a transcriptional repressor that contributes to anterior identity and suppresses Spemann organiser function."
Brickman J.M., Jones C.M., Clements M., Smith J.C., Beddington R.S.P.
Development 127:2303-2315(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Hex acts with beta-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal."
Zamparini A.L., Watts T., Gardner C.E., Tomlinson S.R., Johnston G.I., Brickman J.M.
Development 133:3709-3722(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z21524 Genomic DNA. Translation: CAA79729.1.
AB017132 Genomic DNA. Translation: BAA76714.1.
AF132550 Genomic DNA. Translation: AAF04349.1.
AK014111 mRNA. Translation: BAB29163.1. Frameshift.
AK033664 mRNA. Translation: BAC28416.1.
BC057986 mRNA. Translation: AAH57986.1.
CCDSCCDS29778.1.
PIRS30230.
RefSeqNP_032271.1. NM_008245.3.
UniGeneMm.33896.

3D structure databases

ProteinModelPortalP43120.
SMRP43120. Positions 142-195.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200297. 7 interactions.
MINTMINT-209977.

PTM databases

PhosphoSiteP43120.

Proteomic databases

PRIDEP43120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025944; ENSMUSP00000025944; ENSMUSG00000024986.
GeneID15242.
KEGGmmu:15242.
UCSCuc008hio.1. mouse.

Organism-specific databases

CTD3087.
MGIMGI:96086. Hhex.

Phylogenomic databases

eggNOGNOG252905.
GeneTreeENSGT00730000110534.
HOGENOMHOG000049110.
HOVERGENHBG055460.
InParanoidP43120.
KOK08024.
OMADNSCDQR.
OrthoDBEOG7M0NS8.
PhylomeDBP43120.
TreeFamTF325047.

Gene expression databases

ArrayExpressP43120.
BgeeP43120.
CleanExMM_HHEX.
GenevestigatorP43120.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287843.
PROP43120.
SOURCESearch...

Entry information

Entry nameHHEX_MOUSE
AccessionPrimary (citable) accession number: P43120
Secondary accession number(s): Q544N6, Q9CRV1, Q9R1X2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot