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P43119

- PI2R_HUMAN

UniProt

P43119 - PI2R_HUMAN

Protein

Prostacyclin receptor

Gene

PTGIR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei309 – 3091Not S-palmitoylated

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. guanyl-nucleotide exchange factor activity Source: Reactome

    GO - Biological processi

    1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: BHF-UCL
    2. blood coagulation Source: Reactome
    3. cell-cell signaling Source: ProtInc
    4. G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: ProtInc
    5. negative regulation of platelet-derived growth factor receptor signaling pathway Source: BHF-UCL
    6. negative regulation of smooth muscle cell proliferation Source: BHF-UCL
    7. positive regulation of cAMP biosynthetic process Source: BHF-UCL
    8. positive regulation of cAMP-mediated signaling Source: BHF-UCL
    9. positive regulation of GTPase activity Source: GOC
    10. response to lipopolysaccharide Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_18425. Prostanoid ligand receptors.
    REACT_19327. G alpha (s) signalling events.
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Prostacyclin receptor
    Alternative name(s):
    Prostaglandin I2 receptor
    Short name:
    PGI receptor
    Short name:
    PGI2 receptor
    Prostanoid IP receptor
    Gene namesi
    Name:PTGIR
    Synonyms:PRIPR
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:9602. PTGIR.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: BHF-UCL
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi308 – 3081C → S: Reduced palmitoylation, coupling to G protein unaffected. Abolished palmitoylation and coupling to G protein; when associated with S-311. 1 Publication
    Mutagenesisi309 – 3091C → S: No effect on palmitoylation level. 1 Publication
    Mutagenesisi311 – 3111C → S: Reduced palmitoylation, coupling to G protein unaffected. Abolished palmitoylation and coupling to G protein; when associated with S-308. 1 Publication
    Mutagenesisi383 – 3831C → S: Abolishes isoprenylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA291.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Prostacyclin receptorPRO_0000070075Add
    BLAST
    Propeptidei384 – 3863Removed in mature formSequence AnalysisPRO_0000240004

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi5 ↔ 1651 PublicationPROSITE-ProRule annotation
    Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi92 ↔ 1701 PublicationPROSITE-ProRule annotation
    Lipidationi308 – 3081S-palmitoyl cysteine1 Publication
    Lipidationi311 – 3111S-palmitoyl cysteine1 Publication
    Modified residuei383 – 3831Cysteine methyl esterCurated
    Lipidationi383 – 3831S-farnesyl cysteine1 Publication

    Post-translational modificationi

    Palmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.1 Publication
    Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Methylation, Palmitate, Prenylation

    Proteomic databases

    PaxDbiP43119.
    PRIDEiP43119.

    PTM databases

    PhosphoSiteiP43119.

    Expressioni

    Gene expression databases

    ArrayExpressiP43119.
    BgeeiP43119.
    CleanExiHS_PTGIR.
    GenevestigatoriP43119.

    Interactioni

    Subunit structurei

    Interacts (non-isoprenylated C-terminus) with PDZK1.By similarity

    Protein-protein interaction databases

    BioGridi111711. 14 interactions.
    STRINGi9606.ENSP00000291294.

    Structurei

    3D structure databases

    ProteinModelPortaliP43119.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini39 – 5113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini77 – 9418ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini116 – 13419CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini159 – 18123ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini209 – 23527CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini261 – 27414ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini296 – 38691CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3822Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei52 – 7625Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei95 – 11521Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei135 – 15824Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei182 – 20827Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei236 – 26025Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei275 – 29521Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG305292.
    HOGENOMiHOG000115475.
    HOVERGENiHBG003074.
    InParanoidiP43119.
    KOiK04263.
    OMAiCFLSPAV.
    OrthoDBiEOG72VH6G.
    PhylomeDBiP43119.
    TreeFamiTF324982.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR008365. Prostanoid_rcpt.
    IPR000370. Prostglndn_IP_rcpt.
    [Graphical view]
    PANTHERiPTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF7. PTHR11866:SF7. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01788. PROSTANOIDR.
    PR00856. PRSTNOIDIPR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43119-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADSCRNLTY VRGSVGPATS TLMFVAGVVG NGLALGILSA RRPARPSAFA    50
    VLVTGLAATD LLGTSFLSPA VFVAYARNSS LLGLARGGPA LCDAFAFAMT 100
    FFGLASMLIL FAMAVERCLA LSHPYLYAQL DGPRCARLAL PAIYAFCVLF 150
    CALPLLGLGQ HQQYCPGSWC FLRMRWAQPG GAAFSLAYAG LVALLVAAIF 200
    LCNGSVTLSL CRMYRQQKRH QGSLGPRPRT GEDEVDHLIL LALMTVVMAV 250
    CSLPLTIRCF TQAVAPDSSS EMGDLLAFRF YAFNPILDPW VFILFRKAVF 300
    QRLKLWVCCL CLGPAHGDSQ TPLSQLASGR RDPRAPSAPV GKEGSCVPLS 350
    AWGEGQVEPL PPTQQSSGSA VGTSSKAEAS VACSLC 386
    Length:386
    Mass (Da):40,956
    Last modified:November 1, 1995 - v1
    Checksum:i2B6B0CDBACED1608
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti25 – 251V → M.
    Corresponds to variant rs2229127 [ dbSNP | Ensembl ].
    VAR_024260
    Natural varianti319 – 3191S → W.
    Corresponds to variant rs28590598 [ dbSNP | Ensembl ].
    VAR_061226

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29016 mRNA. Translation: AAA36448.1.
    D25418 mRNA. Translation: BAA05008.1.
    D29634 mRNA. Translation: BAA06110.1.
    D38127 Genomic DNA. Translation: BAA07325.1.
    AY242134 mRNA. Translation: AAO92301.1.
    BC075814 mRNA. Translation: AAH75814.1.
    CCDSiCCDS12686.1.
    PIRiA57066.
    RefSeqiNP_000951.1. NM_000960.3.
    UniGeneiHs.458324.

    Genome annotation databases

    EnsembliENST00000291294; ENSP00000291294; ENSG00000160013.
    GeneIDi5739.
    KEGGihsa:5739.
    UCSCiuc002pex.3. human.

    Polymorphism databases

    DMDMi1172500.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29016 mRNA. Translation: AAA36448.1 .
    D25418 mRNA. Translation: BAA05008.1 .
    D29634 mRNA. Translation: BAA06110.1 .
    D38127 Genomic DNA. Translation: BAA07325.1 .
    AY242134 mRNA. Translation: AAO92301.1 .
    BC075814 mRNA. Translation: AAH75814.1 .
    CCDSi CCDS12686.1.
    PIRi A57066.
    RefSeqi NP_000951.1. NM_000960.3.
    UniGenei Hs.458324.

    3D structure databases

    ProteinModelPortali P43119.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111711. 14 interactions.
    STRINGi 9606.ENSP00000291294.

    Chemistry

    BindingDBi P43119.
    ChEMBLi CHEMBL1995.
    DrugBanki DB01160. Dinoprost Tromethamine.
    DB00917. Dinoprostone.
    DB01240. Epoprostenol.
    DB01088. Iloprost.
    DB00929. Misoprostol.
    GuidetoPHARMACOLOGYi 345.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P43119.

    Polymorphism databases

    DMDMi 1172500.

    Proteomic databases

    PaxDbi P43119.
    PRIDEi P43119.

    Protocols and materials databases

    DNASUi 5739.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000291294 ; ENSP00000291294 ; ENSG00000160013 .
    GeneIDi 5739.
    KEGGi hsa:5739.
    UCSCi uc002pex.3. human.

    Organism-specific databases

    CTDi 5739.
    GeneCardsi GC19M047123.
    HGNCi HGNC:9602. PTGIR.
    MIMi 600022. gene.
    neXtProti NX_P43119.
    PharmGKBi PA291.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG305292.
    HOGENOMi HOG000115475.
    HOVERGENi HBG003074.
    InParanoidi P43119.
    KOi K04263.
    OMAi CFLSPAV.
    OrthoDBi EOG72VH6G.
    PhylomeDBi P43119.
    TreeFami TF324982.

    Enzyme and pathway databases

    Reactomei REACT_18425. Prostanoid ligand receptors.
    REACT_19327. G alpha (s) signalling events.
    REACT_23946. Prostacyclin signalling through prostacyclin receptor.

    Miscellaneous databases

    GeneWikii Prostacyclin_receptor.
    GenomeRNAii 5739.
    NextBioi 22340.
    PROi P43119.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43119.
    Bgeei P43119.
    CleanExi HS_PTGIR.
    Genevestigatori P43119.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR008365. Prostanoid_rcpt.
    IPR000370. Prostglndn_IP_rcpt.
    [Graphical view ]
    PANTHERi PTHR11866. PTHR11866. 1 hit.
    PTHR11866:SF7. PTHR11866:SF7. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01788. PROSTANOIDR.
    PR00856. PRSTNOIDIPR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA for the human prostanoid IP receptor."
      Boie Y., Rushmore T.H., Darmon-Goodwin A., Grygorczyk R., Slipetz D.M., Metters K.M., Abramovitz M.
      J. Biol. Chem. 269:12173-12178(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    2. "Cloning and expression of a cDNA for the human prostacyclin receptor."
      Katsuyama M., Sugimoto Y., Namba T., Irie A., Negishi M., Narumiya S., Ichikawa A.
      FEBS Lett. 344:74-78(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Molecular cloning of human prostacyclin receptor cDNA and its gene expression in the cardiovascular system."
      Nakagawa O., Tanaka I., Usui T., Harada M., Sasaki Y., Itoh H., Yoshimasa T., Namba T., Narumiya S., Nakao K.
      Circulation 90:1643-1647(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Lung.
    4. "Structural organization and chromosomal assignment of the human prostacyclin receptor gene."
      Ogawa Y., Tanaka I., Inoue M., Yoshitake Y., Isse N., Nakagawa O., Usui T., Itoh H., Yoshimasa T., Narumiya S.
      Genomics 27:142-148(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Warren C.N., Aronstam R.S., Sharma S.V.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas.
    7. "Investigation of a functional requirement for isoprenylation by the human prostacyclin receptor."
      Miggin S.M., Lawler O.A., Kinsella B.T.
      Eur. J. Biochem. 269:1714-1725(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ISOPRENYLATION AT CYS-383, MUTAGENESIS OF CYS-383.
    8. "Palmitoylation of the human prostacyclin receptor. Functional implications of palmitoylation and isoprenylation."
      Miggin S.M., Lawler O.A., Kinsella B.T.
      J. Biol. Chem. 278:6947-6958(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-308 AND CYS-311, LACK OF PALMITOYLATION AT CYS-309, MUTAGENESIS OF CYS-308; CYS-309 AND CYS-311.
    9. "Role of extracellular cysteine residues in dimerization/oligomerization of the human prostacyclin receptor."
      Giguere V., Gallant M.A., de Brum-Fernandes A.J., Parent J.-L.
      Eur. J. Pharmacol. 494:11-22(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.

    Entry informationi

    Entry nameiPI2R_HUMAN
    AccessioniPrimary (citable) accession number: P43119
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3