P43118 (PF2R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin F2-alpha receptor
Short name=PGF receptor
Short name=PGF2-alpha receptor

Alternative name(s):
Prostanoid FP receptor

Gene names

Name:

Ptgfr

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prostaglandin F2-alpha (PGF2-alpha). The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. Initiates luteolysis in the corpus luteum.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Highest expression in pregnant ovary. Also found in a low extent in the kidney. In the brain, expressed in astrocytes and oligodendrocytes, and meningeal fibroblasts, but not in migroglia cells.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara extracellular region Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	prostaglandin F receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Prostaglandin F2-alpha receptor		PRO_0000070072
Regions
	Topological domain	1 – 31	31	Extracellular Potential
	Transmembrane	32 – 55	24	Helical; Name=1; Potential
	Topological domain	56 – 69	14	Cytoplasmic Potential
	Transmembrane	70 – 90	21	Helical; Name=2; Potential
	Topological domain	91 – 109	19	Extracellular Potential
	Transmembrane	110 – 131	22	Helical; Name=3; Potential
	Topological domain	132 – 152	21	Cytoplasmic Potential
	Transmembrane	153 – 175	23	Helical; Name=4; Potential
	Topological domain	176 – 198	23	Extracellular Potential
	Transmembrane	199 – 224	26	Helical; Name=5; Potential
	Topological domain	225 – 250	26	Cytoplasmic Potential
	Transmembrane	251 – 267	17	Helical; Name=6; Potential
	Topological domain	268 – 285	18	Extracellular Potential
	Transmembrane	286 – 307	22	Helical; Name=7; Potential
	Topological domain	308 – 366	59	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 186		By similarity
Experimental info
	Sequence conflict	10	1	A → V in AAB19233. Ref.5
	Sequence conflict	113	1	I → M in AAB19233. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P43118 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D44C47F18B326FBA	FASTA	366	40,655
	10 20 30 40 50 60 MSINSSKQPA SSAAGLIANT TCQTENRLSV FFSIIFMTVG IVSNSLAIAI LMKAYQRFRR 70 80 90 100 110 120 KSKASFLLLA SGLVITDFFG HLINGGIAVF VYASDKDWIR FDQSNILCSV FGISMVFSGL 130 140 150 160 170 180 CPLFLGSTMA IERCIGVTNP LFHSTKITSK HVKMILSGVC MFAVFVALLP ILGHRDYQIQ 190 200 210 220 230 240 ASRTWCFYNT EHIEDWEDRF YLLFFSSLGL LALGISFSCN AVTGVTLLRV KFRSQQHRQG 250 260 270 280 290 300 RSHHLEMVIQ LLAIMCVSCV CWSPFLVTMA NIAINGNNSP VTCETTLFAL RMATWNQILD 310 320 330 340 350 360 PWVYILLRKA VLRNLYKLAS RCCGVNIISL HIWELSSIKN SLKVAAISES PAAEKENQQA SSEAGL

References

[1]	"Cloning and expression of a cDNA for rat prostaglandin F2 alpha receptor." Kitanaka J., Hashimoto H., Sugimoto Y., Negishi M., Aino H., Gotoh M., Ichikawa A., Baba A. Prostaglandins 48:31-41(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain cortex.
[2]	"Cloning of the rat and human prostaglandin F2 alpha receptors and the expression of the rat prostaglandin F2 alpha receptor." Lake S., Gullberg H., Wahlqvist J., Sjoegren A.-M., Kinhult A., Lind P., Hellstroem-Lindahl E., Stjernschantz J. FEBS Lett. 355:317-325(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	de Vries C., Neuschaefer-Rube F., Haenecke K., Jungermann K., Pueschel G.P. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[4]	"Characterization and regulation of a mRNA encoding the prostaglandin F2alpha receptor in the rat ovary." Olofsson J.I., Leung C.H.B., Bjurulf E., Ohno T., Selstam G., Peng C., Leung P.C.K. Mol. Cell. Endocrinol. 123:45-52(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[5]	"Negative regulatory activity of a prostaglandin F2 alpha receptor associated protein (FPRP)." Orlicky D.J. Prostaglandins Leukot. Essent. Fatty Acids 54:247-259(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28581 mRNA. Translation: BAA05917.1. S74898 mRNA. Translation: AAB32739.1. X83856 mRNA. Translation: CAA58736.1. U47287 mRNA. Translation: AAB47872.1. U26663 mRNA. Translation: AAB19233.1.
IPI	IPI00197392.
PIR	S51281.
RefSeq	NP_037247.1. NM_013115.1.
UniGene	Rn.97602.
3D structure databases
ProteinModelPortal	P43118.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P43118.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000071195; ENSRNOP00000065253; ENSRNOG00000046468.
GeneID	25652.
KEGG	rno:25652.
Organism-specific databases
CTD	5737.
RGD	3436. Ptgfr.
Phylogenomic databases
GeneTree	ENSGT00690000102015.
HOVERGEN	HBG003621.
KO	K04262.
Gene expression databases
Genevestigator	P43118.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000141. PglndnF_rcpt. IPR008365. Prostanoid_rcpt. [Graphical view]
PANTHER	PTHR11866. PTHR11866. 1 hit. PTHR11866:SF4. PTHR11866:SF4. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01788. PROSTANOIDR. PR00855. PRSTNOIDFPR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607527.

Entry information

Entry name

PF2R_RAT

Accession

Primary (citable) accession number: P43118
Secondary accession number(s): Q62787

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program