P43116 (PE2R2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin E2 receptor EP2 subtype
Short name=PGE receptor EP2 subtype
Short name=PGE2 receptor EP2 subtype

Alternative name(s):
Prostanoid EP2 receptor

Gene names

Name:

PTGER2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Placenta and lung.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to prostaglandin E stimulus Inferred from sequence or structural similarity. Source: BHF-UCL regulation of cell proliferation Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement PubMed 10462542. Source: ProtInc
Molecular_function	prostaglandin E receptor activity Traceable author statement PubMed 10462542. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Prostaglandin E2 receptor EP2 subtype		PRO_0000070054
Regions
	Topological domain	1 – 23	23	Extracellular Potential
	Transmembrane	24 – 47	24	Helical; Name=1; Potential
	Topological domain	48 – 65	18	Cytoplasmic Potential
	Transmembrane	66 – 91	26	Helical; Name=2; Potential
	Topological domain	92 – 111	20	Extracellular Potential
	Transmembrane	112 – 132	21	Helical; Name=3; Potential
	Topological domain	133 – 151	19	Cytoplasmic Potential
	Transmembrane	152 – 176	25	Helical; Name=4; Potential
	Topological domain	177 – 198	22	Extracellular Potential
	Transmembrane	199 – 223	25	Helical; Name=5; Potential
	Topological domain	224 – 262	39	Cytoplasmic Potential
	Transmembrane	263 – 286	24	Helical; Name=6; Potential
	Topological domain	287 – 299	13	Extracellular Potential
	Transmembrane	300 – 323	24	Helical; Name=7; Potential
	Topological domain	324 – 358	35	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Disulfide bond	109 ↔ 187		By similarity
Experimental info
	Sequence conflict	150	1	R → A in AAA61681. Ref.1
	Sequence conflict	150	1	R → A in AAD44177. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P43116 [UniParc]. Last modified November 7, 2003. Version 2. Checksum: B3B0E2AA3CF2E363	FASTA	358	39,761
	10 20 30 40 50 60 MGNASNDSQS EDCETRQWLP PGESPAISSV MFSAGVLGNL IALALLARRW RGDVGCSAGR 70 80 90 100 110 120 RSSLSLFHVL VTELVFTDLL GTCLISPVVL ASYARNQTLV ALAPESRACT YFAFAMTFFS 130 140 150 160 170 180 LATMLMLFAM ALERYLSIGH PYFYQRRVSR SGGLAVLPVI YAVSLLFCSL PLLDYGQYVQ 190 200 210 220 230 240 YCPGTWCFIR HGRTAYLQLY ATLLLLLIVS VLACNFSVIL NLIRMHRRSR RSRCGPSLGS 250 260 270 280 290 300 GRGGPGARRR GERVSMAEET DHLILLAIMT ITFAVCSLPF TIFAYMNETS SRKEKWDLQA 310 320 330 340 350 LRFLSINSII DPWVFAILRP PVLRLMRSVL CCRISLRTQD ATQTSCSTQS DASKQADL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP2 subtype." Regan J.W., Bailey T.J., Pepperl D.J., Pierce K.L., Bogardus A.M., Donello J.E., Fairbairn C.E., Kedzie K.M., Woodward D.F., Gil D.W. Mol. Pharmacol. 46:213-220(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	Oakley C.J. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning, structural characterization, and chromosomal localization of the gene encoding the human prostaglandin E2 receptor EP2 subtype." Smock S.L., Pan L.C., Castleberry T.A., Lu B., Mather R.J., Owen T.A. Gene 237:393-402(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Warren C.N., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	SeattleSNPs variation discovery resource Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19487 mRNA. Translation: AAA61681.1. X83868 mRNA. Translation: CAA58749.1. AF134202, AF134201 Genomic DNA. Translation: AAD44177.1. AY275471 mRNA. Translation: AAP32303.1. DQ398948 Genomic DNA. Translation: ABD48958.1. CH471078 Genomic DNA. Translation: EAW65652.1. CH471078 Genomic DNA. Translation: EAW65654.1. BC093927 mRNA. Translation: AAH93927.1. BC093929 mRNA. Translation: AAH93929.1.
IPI	IPI00016314.
PIR	I38920. S51312.
RefSeq	NP_000947.2. NM_000956.3.
UniGene	Hs.2090.
3D structure databases
ProteinModelPortal	P43116.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43116. 1 interaction.
STRING	9606.ENSP00000245457.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P43116.
Polymorphism databases
DMDM	38258920.
Proteomic databases
PaxDb	P43116.
PRIDE	P43116.
Protocols and materials databases
DNASU	5732.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000245457; ENSP00000245457; ENSG00000125384.
GeneID	5732.
KEGG	hsa:5732.
UCSC	uc001wzr.3. human.
Organism-specific databases
CTD	5732.
GeneCards	GC14P052781.
HGNC	HGNC:9594. PTGER2.
MIM	176804. gene.
neXtProt	NX_P43116.
PharmGKB	PA287.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG275624.
HOGENOM	HOG000115475.
HOVERGEN	HBG003074.
InParanoid	P43116.
KO	K04259.
OMA	AIMTITF.
OrthoDB	EOG45X7WQ.
PhylomeDB	P43116.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P43116.
Bgee	P43116.
CleanEx	HS_PTGER2.
Genevestigator	P43116.
GermOnline	ENSG00000125384. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR008365. Prostanoid_rcpt. IPR001923. Prostglndn_EP2_rcpt. [Graphical view]
PANTHER	PTHR11866. PTHR11866. 1 hit. PTHR11866:SF8. PTHR11866:SF8. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01788. PROSTANOIDR. PR00581. PRSTNOIDEP2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P43116.
ChEMBL	CHEMBL1881.
DrugBank	DB00770. Alprostadil. DB01088. Iloprost.
GenomeRNAi	5732.
NextBio	22298.
SOURCE	Search...

Entry information

Entry name

PE2R2_HUMAN

Accession

Primary (citable) accession number: P43116
Secondary accession number(s): D3DSC0, Q52LG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 7, 2003
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.