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Protein

Prostaglandin E2 receptor EP4 subtype

Gene

Ptger4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: UniProtKB
  • bone development Source: UniProtKB
  • cellular response to glucose stimulus Source: RGD
  • cellular response to interleukin-1 Source: RGD
  • cellular response to mechanical stimulus Source: UniProtKB
  • cellular response to prostaglandin E stimulus Source: RGD
  • chemokinesis Source: RGD
  • ductus arteriosus closure Source: RGD
  • ERK1 and ERK2 cascade Source: UniProtKB
  • female pregnancy Source: RGD
  • JNK cascade Source: UniProtKB
  • maternal process involved in parturition Source: RGD
  • negative regulation of circadian sleep/wake cycle, REM sleep Source: RGD
  • negative regulation of cytokine secretion Source: UniProtKB
  • negative regulation of ductus arteriosus closure Source: RGD
  • negative regulation of endothelin secretion Source: RGD
  • negative regulation of eosinophil extravasation Source: UniProtKB
  • negative regulation of hydrogen peroxide metabolic process Source: RGD
  • negative regulation of inflammatory response Source: UniProtKB
  • negative regulation of integrin activation Source: UniProtKB
  • negative regulation of interleukin-1 alpha secretion Source: RGD
  • negative regulation of nitric-oxide synthase biosynthetic process Source: RGD
  • negative regulation of peptide secretion Source: RGD
  • negative regulation of small intestine smooth muscle contraction Source: RGD
  • negative regulation of tumor necrosis factor production Source: RGD
  • negative regulation of tumor necrosis factor secretion Source: RGD
  • positive regulation of antral ovarian follicle growth Source: RGD
  • positive regulation of bone resorption Source: RGD
  • positive regulation of calcitonin secretion Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of cell adhesion Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of chemokinesis Source: RGD
  • positive regulation of circadian sleep/wake cycle, non-REM sleep Source: RGD
  • positive regulation of cytokine secretion Source: UniProtKB
  • positive regulation of gene expression Source: RGD
  • positive regulation of hyaluronan biosynthetic process Source: RGD
  • positive regulation of inflammatory response Source: UniProtKB
  • positive regulation of interleukin-10 secretion Source: RGD
  • positive regulation of interleukin-8 production Source: RGD
  • positive regulation of matrix metallopeptidase secretion Source: RGD
  • positive regulation of mucus secretion Source: RGD
  • positive regulation of neutrophil extravasation Source: RGD
  • positive regulation of ossification Source: RGD
  • positive regulation of osteoblast differentiation Source: RGD
  • positive regulation of ovarian follicle development Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of smooth muscle cell migration Source: RGD
  • positive regulation of substance P secretion Source: RGD
  • positive regulation of substance P secretion, neurotransmission Source: RGD
  • positive regulation of tyrosine phosphorylation of Stat3 protein Source: RGD
  • positive regulation of urine volume Source: RGD
  • positive regulation of wound healing Source: RGD
  • regulation of circadian sleep/wake cycle, wakefulness Source: RGD
  • regulation of stress fiber assembly Source: UniProtKB
  • response to amino acid Source: RGD
  • response to drug Source: RGD
  • response to interleukin-1 Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to progesterone Source: RGD
  • response to prostaglandin E Source: RGD
  • response to salt Source: RGD
  • response to water-immersion restraint stress Source: RGD
  • T-helper cell differentiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP4 subtype
Short name:
PGE receptor EP4 subtype
Short name:
PGE2 receptor EP4 subtype
Alternative name(s):
Prostanoid EP4 receptor
Gene namesi
Name:Ptger4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628641. Ptger4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919ExtracellularSequence analysisAdd
BLAST
Transmembranei20 – 4324Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini44 – 5512CytoplasmicSequence analysisAdd
BLAST
Transmembranei56 – 7924Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini80 – 9617ExtracellularSequence analysisAdd
BLAST
Transmembranei97 – 11519Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini116 – 13520CytoplasmicSequence analysisAdd
BLAST
Transmembranei136 – 16025Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini161 – 18424ExtracellularSequence analysisAdd
BLAST
Transmembranei185 – 21127Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini212 – 27059CytoplasmicSequence analysisAdd
BLAST
Transmembranei271 – 29828Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini299 – 31517ExtracellularSequence analysisAdd
BLAST
Transmembranei316 – 33520Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini336 – 488153CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • neuronal cell body Source: RGD
  • neuron projection terminus Source: RGD
  • nuclear membrane Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4086.
GuidetoPHARMACOLOGYi343.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Prostaglandin E2 receptor EP4 subtypePRO_0000070068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence analysis
Disulfide bondi92 ↔ 170PROSITE-ProRule annotation
Modified residuei377 – 3771PhosphoserineBy similarity
Modified residuei380 – 3801PhosphoserineBy similarity
Modified residuei382 – 3821PhosphoserineBy similarity
Modified residuei385 – 3851PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP43114.
PRIDEiP43114.

PTM databases

PhosphoSiteiP43114.

Interactioni

Subunit structurei

Interacts with FEM1A.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017886.

Chemistry

BindingDBiP43114.

Structurei

3D structure databases

ProteinModelPortaliP43114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi230 – 2356Poly-Ala

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP43114.
KOiK04261.
OrthoDBiEOG72VH6G.
PhylomeDBiP43114.
TreeFamiTF324982.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPGVNASF SSTPERLNSP VTIPAVMFIF GVVGNLVAIV VLCKSRKEQK
60 70 80 90 100
ETTFYTLVCG LAVTDLLGTL LVSPVTIATY MKGQWPGDQA LCDYSTFILL
110 120 130 140 150
FFGLSGLSII CAMSIERYLA INHAYFYSHY VDKRLAGLTL FAVYASNVLF
160 170 180 190 200
CALPNMGLGR SERQYPGTWC FIDWTTNVTA YAAFSYMYAG FSSFLILATV
210 220 230 240 250
LCNVLVCGAL LRMLRQFMRR TSLGTEQHHA AAAAAVASVA CRGHAAASPA
260 270 280 290 300
LQRLSDFRRR RSFRRIAGAE IQMVILLIAT SLVVLICSIP LVVRVFINQL
310 320 330 340 350
YQPSVVKDIS RNPDLQAIRI ASVNPILDPW IYILLRKTVL SKAIEKIKCL
360 370 380 390 400
FCRIGGSGRD GSAQHCSESR RTSSAMSGHS RSFLSRELRE ISSTSHTLLY
410 420 430 440 450
LPDLTESSLG GKNLLPGTHG MGLTQADTTS LRTLRISETS DSSQGQDSES
460 470 480
VLLVDEVSGS QREEPASKGN SLQVTFPSET LKLSEKCI
Length:488
Mass (Da):53,366
Last modified:November 1, 1995 - v1
Checksum:iC2EE713018723FF4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141L → H in AAB53326 (PubMed:9537820).Curated
Sequence conflicti396 – 3961H → Q in AAB53326 (PubMed:9537820).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28860 mRNA. Translation: BAA06011.1.
U94709 mRNA. Translation: AAB53326.1.
PIRiJC2241.
RefSeqiNP_114465.3. NM_032076.3.
XP_006232058.1. XM_006231996.2.
UniGeneiRn.16062.

Genome annotation databases

GeneIDi84023.
KEGGirno:84023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28860 mRNA. Translation: BAA06011.1.
U94709 mRNA. Translation: AAB53326.1.
PIRiJC2241.
RefSeqiNP_114465.3. NM_032076.3.
XP_006232058.1. XM_006231996.2.
UniGeneiRn.16062.

3D structure databases

ProteinModelPortaliP43114.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017886.

Chemistry

BindingDBiP43114.
ChEMBLiCHEMBL4086.
GuidetoPHARMACOLOGYi343.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP43114.

Proteomic databases

PaxDbiP43114.
PRIDEiP43114.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84023.
KEGGirno:84023.

Organism-specific databases

CTDi5734.
RGDi628641. Ptger4.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000251595.
HOVERGENiHBG053556.
InParanoidiP43114.
KOiK04261.
OrthoDBiEOG72VH6G.
PhylomeDBiP43114.
TreeFamiTF324982.

Miscellaneous databases

NextBioi616589.
PROiP43114.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001758. Prost_EP4_rcpt.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 2 hits.
PTHR11866:SF6. PTHR11866:SF6. 2 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00586. PRSTNOIDEP4R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Molecular cloning and characterization of the four rat prostaglandin E2 prostanoid receptor subtypes."
    Boie Y., Stocco R., Sawyer N., Slipetz D.M., Ungrin M.D., Neuschafer-Rube F., Puschel G.P., Metters K.M., Abramovitz M.
    Eur. J. Pharmacol. 340:227-241(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Kidney.

Entry informationi

Entry nameiPE2R4_RAT
AccessioniPrimary (citable) accession number: P43114
Secondary accession number(s): O08728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 9, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally designated as the EP2 subtype.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.