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Protein

5-aminolevulinate synthase, nonspecific, mitochondrial

Gene

alas1

Organism
Opsanus tau (Oyster toadfish) (Gadus tau)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041SubstrateBy similarity
Binding sitei321 – 3211SubstrateBy similarity
Binding sitei340 – 3401SubstrateBy similarity
Binding sitei373 – 3731Pyridoxal phosphateBy similarity
Binding sitei401 – 4011Pyridoxal phosphateBy similarity
Binding sitei429 – 4291Pyridoxal phosphateBy similarity
Active sitei432 – 4321By similarity
Binding sitei461 – 4611Pyridoxal phosphateBy similarity
Binding sitei462 – 4621Pyridoxal phosphateBy similarity
Binding sitei549 – 5491SubstrateBy similarity

GO - Molecular functioni

  1. 5-aminolevulinate synthase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, nonspecific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-H
Alternative name(s):
5-aminolevulinic acid synthase 1
Delta-ALA synthase 1
Delta-aminolevulinate synthase 1
Gene namesi
Name:alas1
OrganismiOpsanus tau (Oyster toadfish) (Gadus tau)
Taxonomic identifieri8068 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataBatrachoidariaBatrachoididaeOpsanus

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858MitochondrionSequence AnalysisAdd
BLAST
Chaini59 – 6275695-aminolevulinate synthase, nonspecific, mitochondrialPRO_0000001234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei432 – 4321N6-(pyridoxal phosphate)lysineCurated

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP43091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG005954.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVIVRRCPF LARVPQAFFQ QSKKSLAVYA QRCPFMMELA SKPMAPSLAR
60 70 80 90 100
ALCSSSSSQQ KIEDTMSTGE VLKPKAEAKL PVGLATPPSN EAVAPKCPFL
110 120 130 140 150
AAEMGQNNSN VVRQVGVEFQ EDVEEIRTVQ KEVSPAQLEQ PSLIGKTMGE
160 170 180 190 200
EGHQKNLMKS LLKQRPKRVS HLLQDNLPGS FTRFYYDNFF EKKIEEKKSD
210 220 230 240 250
HTYRVFKTVN RLANEFPMAD DFTGSLEDKR EVSVWCSNDY LGMSRHPRVA
260 270 280 290 300
QAIMETLRKH GSGAGGTRNI SGTSKFHVEL EQELADLHRK DAALLFTSCF
310 320 330 340 350
VANDSTLFTL AKMLPGCEIY SDAGNHASMI QGIRNSGAKK FIFRHNDVAH
360 370 380 390 400
LRELLEKGDP TKPKIVAFET VHSMDGAVCP LEEMCDLAHE FGAITFVDEV
410 420 430 440 450
HAVGLYGPRG GGIGDRDGIM HKMDIISGTL GKAFGCVGGY IASTATLVDT
460 470 480 490 500
VRSYAAGFIF TTSLPPMLLA GAKQSIQILK GEEGCTLRRK HQRNVKLLRQ
510 520 530 540 550
MLMDSGLPVV HCPSHIIPIR VSDAEKNTKV CDLMMSHHNI YVQAINYPTV
560 570 580 590 600
ARGDELLRIA PTPHHTPEMM KYFVDRLVQT WKEVGLELKP HSSAECTFCQ
610 620
QPLHFEVMNE REKSYFSGLS HLVSVCA
Length:627
Mass (Da):69,691
Last modified:November 1, 1995 - v1
Checksum:iB94FB3406D9FCCAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35915 mRNA. Translation: AAA49435.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35915 mRNA. Translation: AAA49435.1.

3D structure databases

ProteinModelPortaliP43091.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005954.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Housekeeping form of 5-aminolevulinate synthase in the marine fish, Opsanus tau."
    Cornell N.W.
    Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiHEM1_OPSTA
AccessioniPrimary (citable) accession number: P43091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.