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Protein

5-aminolevulinate synthase, erythroid-specific, mitochondrial

Gene

alas2

Organism
Opsanus tau (Oyster toadfish) (Gadus tau)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from glycine.
Proteins known to be involved in this subpathway in this organism are:
  1. 5-aminolevulinate synthase, erythroid-specific, mitochondrial (alas2), 5-aminolevulinate synthase, nonspecific, mitochondrial (alas1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from glycine, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei158SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei327Pyridoxal phosphateBy similarity1
Binding sitei355Pyridoxal phosphateBy similarity1
Binding sitei383Pyridoxal phosphateBy similarity1
Active sitei386By similarity1
Binding sitei415Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei416Pyridoxal phosphate; shared with dimeric partnerBy similarity1
Binding sitei503SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Heme biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Names & Taxonomyi

Protein namesi
Recommended name:
5-aminolevulinate synthase, erythroid-specific, mitochondrial (EC:2.3.1.37)
Short name:
ALAS-E
Alternative name(s):
5-aminolevulinic acid synthase 2
Delta-ALA synthase 2
Delta-aminolevulinate synthase 2
Gene namesi
Name:alas2
OrganismiOpsanus tau (Oyster toadfish) (Gadus tau)
Taxonomic identifieri8068 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataBatrachoidariaBatrachoididaeOpsanus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000001228? – 5825-aminolevulinate synthase, erythroid-specific, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei386N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP43090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG005954.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFLHHCPF LKSMPKPALR RRVPALLSLA DRCPVIVHQV CISRLHILET
60 70 80 90 100
KLDVSPTQPK RQRLSLLDQK RLFAQTATQV AVSVSKGCPF VSSQIGMVRA
110 120 130 140 150
SPEVQEDVQA DLKSPVLPTP PQTGITQLLK DNMVGPSFDY DNFFNEKIAE
160 170 180 190 200
KKRDHTYRVF KTVNRNAVVF PFAEDYSVSD RQGSQVSVWC SNDYLGMSRH
210 220 230 240 250
PRVLEAIREV LERHGAGAGG TRNISGTSKY HVTLEKELAH LHQKDAALVF
260 270 280 290 300
SSCFVANDST LFTLAKMLPG CHIYSDAGNH ASMIQGIRNS GAKRFIFRHN
310 320 330 340 350
DSRHLEELLQ QSDPKTPKIV AFETVHSMDG AICPLEELCD VAHRHGALTF
360 370 380 390 400
VDEVHAVGLY GAHGAGVGER DNVMHKIDIV SGTLGKAFGC VGGYVASSAA
410 420 430 440 450
LVDTVRSFAA GFIFTTSLPP MILAGALESV RVLKSPEGQL LRRAHQRNVK
460 470 480 490 500
YMRQLLMDKG LPVVNCPSHI IPIRVGNAEL NTKVCDSLLE KHNIYVQAIN
510 520 530 540 550
YPTVPRGQEL LRLAPSPHHH PAMMEYFVDK LVEVWQEAGL LLNGPATVSC
560 570 580
TFCDRPLHFD LMSEWEKSYF GNMEPQYITM SA
Length:582
Mass (Da):64,531
Last modified:November 1, 1995 - v1
Checksum:iFB066EEF72E146C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02632 mRNA. Translation: AAA60729.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02632 mRNA. Translation: AAA60729.1.

3D structure databases

ProteinModelPortaliP43090.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005954.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00375.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
IPR015118. 5aminolev_synth_preseq.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
PF09029. Preseq_ALAS. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM0_OPSTA
AccessioniPrimary (citable) accession number: P43090
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two delta-ALA synthases in vertebrates: an erythroid- specific form and one (housekeeping) which is expressed in all tissues.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.