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P43089

- HEM1_PARDP

UniProt

P43089 - HEM1_PARDP

Protein

5-aminolevulinate synthase

Gene

hemA

Organism
Paracoccus denitrificans (strain Pd 1222)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (06 Mar 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei156 – 1561SubstrateBy similarity
    Binding sitei189 – 1891Pyridoxal phosphateBy similarity
    Binding sitei217 – 2171Pyridoxal phosphateBy similarity
    Binding sitei245 – 2451Pyridoxal phosphateBy similarity
    Active sitei248 – 2481By similarity
    Binding sitei277 – 2771Pyridoxal phosphateBy similarity
    Binding sitei278 – 2781Pyridoxal phosphateBy similarity
    Binding sitei365 – 3651SubstrateBy similarity

    GO - Molecular functioni

    1. 5-aminolevulinate synthase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Heme biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciPDEN318586:GCVQ-1848-MONOMER.
    UniPathwayiUPA00251; UER00375.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-aminolevulinate synthase (EC:2.3.1.37)
    Alternative name(s):
    5-aminolevulinic acid synthase
    Delta-ALA synthase
    Delta-aminolevulinate synthase
    Gene namesi
    Name:hemA
    Ordered Locus Names:Pden_1822
    OrganismiParacoccus denitrificans (strain Pd 1222)
    Taxonomic identifieri318586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus
    ProteomesiUP000000361: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4094095-aminolevulinate synthasePRO_0000163826Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei248 – 2481N6-(pyridoxal phosphate)lysineCurated

    Interactioni

    Protein-protein interaction databases

    STRINGi318586.Pden_1822.

    Structurei

    3D structure databases

    ProteinModelPortaliP43089.
    SMRiP43089. Positions 1-397.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0156.
    HOGENOMiHOG000221020.
    KOiK00643.
    OrthoDBiEOG6Q8HZD.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01821. 5aminolev_synth. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43089-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSAALDQA IGKLHEEGRY RTFIDIERRK GAYPQAVWTR PDGTETRITV    50
    WCGNDYLGMG QHPVVLAAMH EALDATGAGS GGTRNISGTT VYHKRLEAEL 100
    SDLHGKEAAL VFSSAYIAND ATLSTLRKLF PGLIIYSDEL NHASMIEGIK 150
    RFDGAKRIFR HNDVAHLREL LAADDPEAPK LIAFESIYSM DGDFGPIKAI 200
    CDLADEFNAL TYLDEVHAVG MYGPRGGGVA ERDGLSHRID IFNGTLGKAF 250
    GVFGGYIAAS ARMVDAIRSY APGFIFTTSL PPAVAAGAAA SIAFLKTAEG 300
    QLLRDQQQLN ARILKMRLRG LGMPIMDHGS HIVPVHVGNP VHCKALSDML 350
    LADFGIYVQP INFPTVPRGT ERLRFTPSPV HDPKQIDHLV KAMDSLWSQC 400
    KLNRSTSAA 409
    Length:409
    Mass (Da):44,560
    Last modified:March 6, 2007 - v2
    Checksum:i1A2EEE178D58B32E
    GO

    Sequence cautioni

    The sequence ABL69919.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351Q → T in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti101 – 1011S → A in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti115 – 1151A → V in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti152 – 1554FDGA → STAP in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti165 – 1651A → G in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti195 – 1951G → A in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti199 – 1991A → E in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti247 – 2471G → A in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti258 – 2603AAS → GFG in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti288 – 2881A → V in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti302 – 3032LL → FV in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti311 – 3133ARI → GRL in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti321 – 3211L → A in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti325 – 3251I → V in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti355 – 3551G → S in AAA62279. (PubMed:7928952)Curated
    Sequence conflicti377 – 3771P → A in AAA62279. (PubMed:7928952)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12508 Genomic DNA. Translation: AAA62279.1.
    CP000489 Genomic DNA. Translation: ABL69919.1. Different initiation.
    RefSeqiYP_915615.1. NC_008686.1.

    Genome annotation databases

    EnsemblBacteriaiABL69919; ABL69919; Pden_1822.
    GeneIDi4578534.
    KEGGipde:Pden_1822.
    PATRICi22854745. VBIParDen97112_1755.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12508 Genomic DNA. Translation: AAA62279.1 .
    CP000489 Genomic DNA. Translation: ABL69919.1 . Different initiation.
    RefSeqi YP_915615.1. NC_008686.1.

    3D structure databases

    ProteinModelPortali P43089.
    SMRi P43089. Positions 1-397.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 318586.Pden_1822.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL69919 ; ABL69919 ; Pden_1822 .
    GeneIDi 4578534.
    KEGGi pde:Pden_1822.
    PATRICi 22854745. VBIParDen97112_1755.

    Phylogenomic databases

    eggNOGi COG0156.
    HOGENOMi HOG000221020.
    KOi K00643.
    OrthoDBi EOG6Q8HZD.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00375 .
    BioCyci PDEN318586:GCVQ-1848-MONOMER.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR010961. 4pyrrol_synth_NH2levulA_synth.
    IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01821. 5aminolev_synth. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential reduction in soluble and membrane-bound c-type cytochrome contents in a Paracoccus denitrificans mutant partially deficient in 5-aminolevulinate synthase activity."
      Page M.D., Ferguson S.J.
      J. Bacteriol. 176:5919-5928(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pd 1222.

    Entry informationi

    Entry nameiHEM1_PARDP
    AccessioniPrimary (citable) accession number: P43089
    Secondary accession number(s): A1B325
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3