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P43087

- HEM2_SYNE7

UniProt

P43087 - HEM2_SYNE7

Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 1 zinc ion per monomer.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi119 – 1191Zinc; catalyticBy similarity
    Metal bindingi121 – 1211Zinc; catalyticBy similarity
    Metal bindingi129 – 1291Zinc; catalyticBy similarity
    Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
    Binding sitei208 – 2081Substrate 1By similarity
    Binding sitei220 – 2201Substrate 1By similarity
    Metal bindingi236 – 2361MagnesiumBy similarity
    Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
    Binding sitei277 – 2771Substrate 2By similarity
    Binding sitei316 – 3161Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciSYNEL:SYNPCC7942_1792-MONOMER.
    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
    Short name:
    ALAD
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:hemB
    Ordered Locus Names:Synpcc7942_1792
    OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
    Taxonomic identifieri1140 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000002717: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Delta-aminolevulinic acid dehydratasePRO_0000140520Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    STRINGi1140.Synpcc7942_1792.

    Structurei

    3D structure databases

    ProteinModelPortaliP43087.
    SMRiP43087. Positions 5-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OrthoDBiEOG6VXFCB.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43087-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFPTHRPRRL RSSETLRRMV RETTLTSADF IYPLFAVPGE GVAKEVTSMP    50
    GVYQLSIDKI VEEAKEVYDL GIPSIILFGI PTDKDNDATG AWHDCGIVQK 100
    AATAVKEAVP ELIVAADTCL CEYTPHGHCG YLEVGDLSGR VLNDPTLELL 150
    RKTAVSQAKA GADIIAPSGM MDGFVATIRD ALDEAGFSDT PIMAYSAKYA 200
    SAYYGPFRDA AESTPQFGDR RTYQMDPGNS REALKEVELD VAEGADIVMV 250
    KPALSYMDII CRIKETTDLP VAAYNVSGEY SMVKAAALNG WIDEERVVLE 300
    TLTSFKRAGA DLILTYHAKD AARWLA 326
    Length:326
    Mass (Da):35,574
    Last modified:April 18, 2006 - v2
    Checksum:i0C4F44658CEB37BF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti287 – 2871A → R in CAA49892. (PubMed:8123787)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70434 Genomic DNA. Translation: CAA49892.1.
    CP000100 Genomic DNA. Translation: ABB57822.1.
    RefSeqiYP_400809.1. NC_007604.1.

    Genome annotation databases

    EnsemblBacteriaiABB57822; ABB57822; Synpcc7942_1792.
    GeneIDi3774367.
    KEGGisyf:Synpcc7942_1792.
    PATRICi23789009. VBISynElo51371_2028.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70434 Genomic DNA. Translation: CAA49892.1 .
    CP000100 Genomic DNA. Translation: ABB57822.1 .
    RefSeqi YP_400809.1. NC_007604.1.

    3D structure databases

    ProteinModelPortali P43087.
    SMRi P43087. Positions 5-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 1140.Synpcc7942_1792.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB57822 ; ABB57822 ; Synpcc7942_1792 .
    GeneIDi 3774367.
    KEGGi syf:Synpcc7942_1792.
    PATRICi 23789009. VBISynElo51371_2028.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OrthoDBi EOG6VXFCB.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .
    BioCyci SYNEL:SYNPCC7942_1792-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterisation of genes for tetrapyrrole biosynthesis from the cyanobacterium Anacystis nidulans R2."
      Jones M.C., Jenkins J.M., Smith A.G., Howe C.J.
      Plant Mol. Biol. 24:435-448(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 7942.

    Entry informationi

    Entry nameiHEM2_SYNE7
    AccessioniPrimary (citable) accession number: P43087
    Secondary accession number(s): Q31M97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3