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P43087

- HEM2_SYNE7

UniProt

P43087 - HEM2_SYNE7

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Protein

Delta-aminolevulinic acid dehydratase

Gene

hemB

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 1 zinc ion per monomer.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc; catalyticBy similarity
Metal bindingi121 – 1211Zinc; catalyticBy similarity
Metal bindingi129 – 1291Zinc; catalyticBy similarity
Active sitei198 – 1981Schiff-base intermediate with substrateBy similarity
Binding sitei208 – 2081Substrate 1By similarity
Binding sitei220 – 2201Substrate 1By similarity
Metal bindingi236 – 2361MagnesiumBy similarity
Active sitei251 – 2511Schiff-base intermediate with substrateBy similarity
Binding sitei277 – 2771Substrate 2By similarity
Binding sitei316 – 3161Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_1792-MONOMER.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:hemB
Ordered Locus Names:Synpcc7942_1792
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002717: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Delta-aminolevulinic acid dehydratasePRO_0000140520Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi1140.Synpcc7942_1792.

Structurei

3D structure databases

ProteinModelPortaliP43087.
SMRiP43087. Positions 5-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OrthoDBiEOG6VXFCB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43087-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFPTHRPRRL RSSETLRRMV RETTLTSADF IYPLFAVPGE GVAKEVTSMP
60 70 80 90 100
GVYQLSIDKI VEEAKEVYDL GIPSIILFGI PTDKDNDATG AWHDCGIVQK
110 120 130 140 150
AATAVKEAVP ELIVAADTCL CEYTPHGHCG YLEVGDLSGR VLNDPTLELL
160 170 180 190 200
RKTAVSQAKA GADIIAPSGM MDGFVATIRD ALDEAGFSDT PIMAYSAKYA
210 220 230 240 250
SAYYGPFRDA AESTPQFGDR RTYQMDPGNS REALKEVELD VAEGADIVMV
260 270 280 290 300
KPALSYMDII CRIKETTDLP VAAYNVSGEY SMVKAAALNG WIDEERVVLE
310 320
TLTSFKRAGA DLILTYHAKD AARWLA
Length:326
Mass (Da):35,574
Last modified:April 18, 2006 - v2
Checksum:i0C4F44658CEB37BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti287 – 2871A → R in CAA49892. (PubMed:8123787)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70434 Genomic DNA. Translation: CAA49892.1.
CP000100 Genomic DNA. Translation: ABB57822.1.
RefSeqiYP_400809.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB57822; ABB57822; Synpcc7942_1792.
GeneIDi3774367.
KEGGisyf:Synpcc7942_1792.
PATRICi23789009. VBISynElo51371_2028.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70434 Genomic DNA. Translation: CAA49892.1 .
CP000100 Genomic DNA. Translation: ABB57822.1 .
RefSeqi YP_400809.1. NC_007604.1.

3D structure databases

ProteinModelPortali P43087.
SMRi P43087. Positions 5-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 1140.Synpcc7942_1792.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB57822 ; ABB57822 ; Synpcc7942_1792 .
GeneIDi 3774367.
KEGGi syf:Synpcc7942_1792.
PATRICi 23789009. VBISynElo51371_2028.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OrthoDBi EOG6VXFCB.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .
BioCyci SYNEL:SYNPCC7942_1792-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterisation of genes for tetrapyrrole biosynthesis from the cyanobacterium Anacystis nidulans R2."
    Jones M.C., Jenkins J.M., Smith A.G., Howe C.J.
    Plant Mol. Biol. 24:435-448(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7942.

Entry informationi

Entry nameiHEM2_SYNE7
AccessioniPrimary (citable) accession number: P43087
Secondary accession number(s): Q31M97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 18, 2006
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3