Reviewed, UniProtKB/Swiss-Prot P43084 (EBP1_CANAL)

Names and origin

Protein names

Recommended name:
    Probable NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Estrogen-binding protein
      Short name=EBP

Gene names

Name:

EBP1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidoreductase that binds mammalian estrogens with high affinity.
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor.
Cofactor	FMN By similarity.
Developmental stage	The expression is high during the lag and early logarithmic phases of growth, and low at or near stationary phase.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 407	406	Probable NADPH dehydrogenase		PRO_0000194479
Regions
	Region	201 – 204	4	Substrate-binding By similarity
Sites
	Active site	206	1	Proton donor By similarity
	Binding site	124	1	FMN By similarity
	Binding site	254	1	FMN By similarity
	Binding site	357	1	FMN By similarity
	Site	225	1	May interact with the steroid

Sequences

	Sequence		Length	Mass (Da)
	P43084-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DDDDB7BD0220639A	FASTA	407	46,074
	10 20 30 40 50 60 MTIESTNSFV VPSDTKLIDV TPLGSTKLFQ PIKVGNNVLP QRIAYVPTTR FRASKDHIPS 70 80 90 100 110 120 DLQLNYYNAR SQYPGTLIIT EATFASERGG IDLHVPGIYN DAQAKSWKKI NEAIHGNGSF 130 140 150 160 170 180 SSVQLWYLGR VANAKDLKDS GLPLIAPSAV YWDENSEKLA KEAGNELRAL TEEEIDHIVE 190 200 210 220 230 240 VEYPNAAKHA LEAGFDYVEI HGAHGYLLDQ FLNLASNKRT DKYGCGSIEN RARLLLRVVD 250 260 270 280 290 300 KLIEVVGANR LALRLSPWAS FQGMEIEGEE IHSYILQQLQ QRADNGQQLA YISLVEPRVT 310 320 330 340 350 360 GIYDVSLKDQ QGRSNEFAYK IWKGNFIRAG NYTYDAPEFK TLINDLKNDR SIIGFSRFFT 370 380 390 400 SNPDLVEKLK LGKPLNYYNR EEFYKYYNYG YNSYDESEKQ VIGKPLA

References

[1]

"Candida albicans estrogen-binding protein gene encodes an oxidoreductase that is inhibited by estradiol." Madani N.D., Malloy P.J., Rodriguez-Pombo P., Krishnan A.V., Feldman D. Proc. Natl. Acad. Sci. U.S.A. 91:922-926(1994) [PubMed: 8302868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-10 AND 265-279. Strain: 158 / STN 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25759 Unassigned RNA. Translation: AAA18013.1.
PIR	A36990.
3D structure databases
HSSP	HSSP built from PDB template 1OYC based on UniProtKB Q02899.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.99.1. 1124.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBP1_CANAL

Accession

Primary (citable) accession number: P43084

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)