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Protein

Hevein-like preproprotein

Gene

HEL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Fungal growth inhibitors. Neither CB-HEL nor CD-HEL have chitinase activity, but both have antimicrobial activities. CD-HEL has RNase, but no DNase activity.1 Publication

GO - Molecular functioni

  • chitin binding Source: TAIR
  • ribonuclease activity Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: InterPro
  • defense response to fungus, incompatible interaction Source: TAIR
  • killing of cells of other organism Source: UniProtKB-KW
  • response to ethylene Source: TAIR
  • response to herbivore Source: TAIR
  • response to salt stress Source: TAIR
  • response to virus Source: TAIR
  • systemic acquired resistance Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide, Hydrolase, Nuclease, Pathogenesis-related protein

Keywords - Biological processi

Plant defense

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BioCyciARA:AT3G04720-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Hevein-like preproprotein
Cleaved into the following 2 chains:
Alternative name(s):
RNase
Gene namesi
Name:HEL
Ordered Locus Names:At3g04720
ORF Names:F7O18.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G04720.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 212CD-HELPRO_0000421569
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 212191Hevein-like preproproteinPRO_0000005284Add
BLAST
Chaini22 – ?CB-HELPRO_0000421568

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 391 Publication
Disulfide bondi33 ↔ 451 Publication
Disulfide bondi38 ↔ 521 Publication
Disulfide bondi58 ↔ 621 Publication
Disulfide bondi100 ↔ 132PROSITE-ProRule annotation
Disulfide bondi121 ↔ 155PROSITE-ProRule annotation
Disulfide bondi135 ↔ 191PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP43082.
PRIDEiP43082.

Expressioni

Inductioni

Up-regulated by ethylene, methyl jasmonate, wounding and virus infection. Weakly induced by salicylic acid and 2,6-dichlorisonicotinic acid.2 Publications

Gene expression databases

GenevisibleiP43082. AT.

Interactioni

Subunit structurei

CB-HEL interacts strongly with a fungal fruiting body lectin.1 Publication

Protein-protein interaction databases

IntActiP43082. 1 interaction.
STRINGi3702.AT3G04720.1.

Structurei

3D structure databases

ProteinModelPortaliP43082.
SMRiP43082. Positions 22-62, 72-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 6443Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST
Domaini72 – 193122BarwinPROSITE-ProRule annotationAdd
BLAST

Domaini

The C-terminal vacuolar sorting signal (VSS) (194-212) is required for vacuolar localization.1 Publication

Sequence similaritiesi

Contains 1 barwin domain.PROSITE-ProRule annotation
Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000090989.
InParanoidiP43082.
OMAiQFVDCGN.
OrthoDBiEOG09360QTL.
PhylomeDBiP43082.

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
3.30.60.10. 1 hit.
InterProiIPR018226. Barwin_CS.
IPR001153. Barwin_dom.
IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF00967. Barwin. 1 hit.
PF00187. Chitin_bind_1. 1 hit.
[Graphical view]
PRINTSiPR00602. BARWIN.
PR00451. CHITINBINDNG.
ProDomiPD004535. Barwin. 1 hit.
PD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00771. BARWIN_1. 1 hit.
PS00772. BARWIN_2. 1 hit.
PS51174. BARWIN_3. 1 hit.
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIRLSITII LLSYTVATVA GQQCGRQGGG RTCPGNICCS QYGYCGTTAD
60 70 80 90 100
YCSPTNNCQS NCWGSGPSGP GESASNVRAT YHFYNPAQNN WDLRAVSAYC
110 120 130 140 150
STWDADKPYA WRSKYGWTAF CGPAGPRGQA SCGKCLRVKN TRTNAAVTVR
160 170 180 190 200
IVDQCSNGGL DLDVAMFNQI DTDGFGYQQG HLIVDYQFVD CGNELIGQPD
210
SRNMLVSAID RV
Length:212
Mass (Da):22,937
Last modified:November 1, 1995 - v1
Checksum:i1EDAD140A21AA692
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01880 mRNA. Translation: AAA20642.1.
AC011437 Genomic DNA. Translation: AAF04912.1.
CP002686 Genomic DNA. Translation: AEE74125.1.
AF370536 mRNA. Translation: AAK48963.1.
BT000046 mRNA. Translation: AAN15365.1.
AY088644 mRNA. Translation: AAM66966.1.
RefSeqiNP_187123.1. NM_111344.5.
UniGeneiAt.252.

Genome annotation databases

EnsemblPlantsiAT3G04720.1; AT3G04720.1; AT3G04720.
GeneIDi819632.
GrameneiAT3G04720.1; AT3G04720.1; AT3G04720.
KEGGiath:AT3G04720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01880 mRNA. Translation: AAA20642.1.
AC011437 Genomic DNA. Translation: AAF04912.1.
CP002686 Genomic DNA. Translation: AEE74125.1.
AF370536 mRNA. Translation: AAK48963.1.
BT000046 mRNA. Translation: AAN15365.1.
AY088644 mRNA. Translation: AAM66966.1.
RefSeqiNP_187123.1. NM_111344.5.
UniGeneiAt.252.

3D structure databases

ProteinModelPortaliP43082.
SMRiP43082. Positions 22-62, 72-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP43082. 1 interaction.
STRINGi3702.AT3G04720.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.

Proteomic databases

PaxDbiP43082.
PRIDEiP43082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G04720.1; AT3G04720.1; AT3G04720.
GeneIDi819632.
GrameneiAT3G04720.1; AT3G04720.1; AT3G04720.
KEGGiath:AT3G04720.

Organism-specific databases

TAIRiAT3G04720.

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000090989.
InParanoidiP43082.
OMAiQFVDCGN.
OrthoDBiEOG09360QTL.
PhylomeDBiP43082.

Enzyme and pathway databases

BioCyciARA:AT3G04720-MONOMER.

Miscellaneous databases

PROiP43082.

Gene expression databases

GenevisibleiP43082. AT.

Family and domain databases

Gene3Di2.40.40.10. 1 hit.
3.30.60.10. 1 hit.
InterProiIPR018226. Barwin_CS.
IPR001153. Barwin_dom.
IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF00967. Barwin. 1 hit.
PF00187. Chitin_bind_1. 1 hit.
[Graphical view]
PRINTSiPR00602. BARWIN.
PR00451. CHITINBINDNG.
ProDomiPD004535. Barwin. 1 hit.
PD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00771. BARWIN_1. 1 hit.
PS00772. BARWIN_2. 1 hit.
PS51174. BARWIN_3. 1 hit.
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEVL_ARATH
AccessioniPrimary (citable) accession number: P43082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.