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Protein

Guanylyl cyclase-activating protein 1

Gene

Guca1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of retinal guanylyl cyclase is a key event in recovery of the dark state of rod photoreceptors following light exposure.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi64 – 75121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi100 – 111122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi144 – 155123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • calcium sensitive guanylate cyclase activator activity Source: MGI
  • guanylate cyclase regulator activity Source: MGI

GO - Biological processi

  • cellular response to calcium ion Source: UniProtKB
  • phototransduction Source: MGI
  • regulation of guanylate cyclase activity Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase-activating protein 1
Short name:
GCAP 1
Alternative name(s):
Guanylate cyclase activator 1A
Gene namesi
Name:Guca1a
Synonyms:Gcap, Gcap1, Guca1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:102770. Guca1a.

Subcellular locationi

GO - Cellular componenti

  • photoreceptor inner segment Source: Ensembl
  • photoreceptor outer segment Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 202201Guanylyl cyclase-activating protein 1PRO_0000073804Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Modified residuei3 – 31Deamidated asparagineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP43081.
PRIDEiP43081.

Expressioni

Tissue specificityi

Retina; rod and cone outer segments.

Gene expression databases

CleanExiMM_GUCA1A.
GenevisibleiP43081. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060027.

Structurei

3D structure databases

ProteinModelPortaliP43081.
SMRiP43081. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 4936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini51 – 8636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 12236EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini131 – 16636EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Domaini

Binds three calcium ions (via EF-hands 2, 3 and 4) when calcium levels are high. Binds Mg2+ when calcium levels are low.By similarity

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP43081.
KOiK08328.
OMAiINPCSDS.
OrthoDBiEOG7BP83V.
TreeFamiTF333971.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43081-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIMEGKSV EELSSTECHQ WYKKFMTECP SGQLTLYEFR QFFGLKNLSP
60 70 80 90 100
SASQYVEQMF ETFDFNKDGY IDFMEYVAAL SLVLKGKVEQ KLRWYFKLYD
110 120 130 140 150
VDGNGCIDRD ELLTIIRAIR TINPWSDSSM SAEEFTDTVF AKIDINGDGE
160 170 180 190 200
LSLEEFMEGV QKDQMLLDTL TRSLDLTGIV RRLQNGEHEE AGTGDLAAEA

AG
Length:202
Mass (Da):22,986
Last modified:October 3, 2012 - v3
Checksum:iEC76CBF7C518C76B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41I → V in AAA60716 (PubMed:7983048).Curated
Sequence conflicti29 – 291C → V in AAA60716 (PubMed:7983048).Curated
Sequence conflicti194 – 1941G → S in AAA60716 (PubMed:7983048).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36860 mRNA. Translation: AAA60716.1.
AC112683 Genomic DNA. No translation available.
CH466559 Genomic DNA. Translation: EDL23558.1.
CH466559 Genomic DNA. Translation: EDL23559.1.
CH466559 Genomic DNA. Translation: EDL23560.1.
BC026834 mRNA. Translation: AAH26834.1.
BC031810 mRNA. Translation: AAH31810.1.
CCDSiCCDS28848.1.
PIRiB55331.
RefSeqiNP_032215.2. NM_008189.3.
XP_011244598.1. XM_011246296.1.
UniGeneiMm.16224.

Genome annotation databases

EnsembliENSMUST00000059348; ENSMUSP00000060027; ENSMUSG00000023982.
GeneIDi14913.
KEGGimmu:14913.
UCSCiuc008cvc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36860 mRNA. Translation: AAA60716.1.
AC112683 Genomic DNA. No translation available.
CH466559 Genomic DNA. Translation: EDL23558.1.
CH466559 Genomic DNA. Translation: EDL23559.1.
CH466559 Genomic DNA. Translation: EDL23560.1.
BC026834 mRNA. Translation: AAH26834.1.
BC031810 mRNA. Translation: AAH31810.1.
CCDSiCCDS28848.1.
PIRiB55331.
RefSeqiNP_032215.2. NM_008189.3.
XP_011244598.1. XM_011246296.1.
UniGeneiMm.16224.

3D structure databases

ProteinModelPortaliP43081.
SMRiP43081. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060027.

Proteomic databases

PaxDbiP43081.
PRIDEiP43081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059348; ENSMUSP00000060027; ENSMUSG00000023982.
GeneIDi14913.
KEGGimmu:14913.
UCSCiuc008cvc.1. mouse.

Organism-specific databases

CTDi2978.
MGIiMGI:102770. Guca1a.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP43081.
KOiK08328.
OMAiINPCSDS.
OrthoDBiEOG7BP83V.
TreeFamiTF333971.

Enzyme and pathway databases

ReactomeiR-MMU-2514859. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

PROiP43081.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GUCA1A.
GenevisibleiP43081. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of human and mouse photoreceptor guanylate cyclase-activating protein (GCAP) and chromosomal localization of the human gene."
    Subbaraya I., Ruiz C.C., Helekar B.S., Zhao X., Gorczyca W.A., Pettenati M.J., Rao P.N., Palczewski K., Baehr W.
    J. Biol. Chem. 269:31080-31089(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiGUC1A_MOUSE
AccessioniPrimary (citable) accession number: P43081
Secondary accession number(s): Q8R0H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 3, 2012
Last modified: June 8, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.