Reviewed, UniProtKB/Swiss-Prot P43065 (LYS1_CANAL)

Names and origin

Protein names

Recommended name:
    Saccharopine dehydrogenase [NAD+, L-lysine-forming]
      Short name=SDH
    EC=1.5.1.7
Alternative name(s):
    Lysine--2-oxoglutarate reductase

Gene names

Name:

LYS1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungi route): step 3/3.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Saccharopine dehydrogenase [NAD+, L-lysine-forming]		PRO_0000199010
Sites
	Active site	217	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43065-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A3620191DF04B88F	FASTA	382	42,394
	10 20 30 40 50 60 MSKSPVILHL RAETKPLEAR AALTPSTTKQ LLDAGFEIYV EESSQSTFDI KEYEAVGAKI 70 80 90 100 110 120 VPEGSWKTAP KERIIFGLKE LPENETFPLI HEHIQFAHCY KDQAGWQDVL KRFPQGNGIL 130 140 150 160 170 180 YDLEFLENDQ GRRVAAFGFY AGFAGAAIGV LDWSFKQLNG NTKGTKGEGE GGELPGVTPY 190 200 210 220 230 240 PNENELIKDV KIELEKALTK NGGQYPKCLV IGALGRCGSG AIDLFKKIGI PDDNIAKWDM 250 260 270 280 290 300 AETAKGGPFQ EIVDSDIFIN CIYLSKPIPP FINKEILNNE NRKLTTIVDV SADTTNPHNP 310 320 330 340 350 360 IPVYEIATVF NEPTVEVKLD KGPKLSVCSI DHLPSLLPRE ASEFFAKDLM PSLLELPNRD 370 380 TSPVWVRAKQ LFDKHVARLD KE

References

[1]	"Molecular and functional analysis of the LYS1 gene of Candida albicans." Garrad R.C., Schmidt T.M., Bhattacharjee J.K. Infect. Immun. 62:5027-5031(1994) [PubMed: 7927784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13233 Genomic DNA. Translation: AAA21362.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.7. 1124.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR007886. Ala_DH/PNT_N. [Graphical view]
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_CANAL

Accession

Primary (citable) accession number: P43065

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)