P43063 (CDK1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 1
Short name=CDK1
EC=2.7.11.22

Alternative name(s):
Cell division control protein 28
Cell division protein kinase 2

Gene names

Name:	CDC28
Synonyms:	CDK1
ORF Names:	CaO19.11337, CaO19.3856

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein is essential for the completion of the start, the controlling event, in the cell cycle.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-17 or Tyr-18 inactivates the enzyme, while phosphorylation at Thr-166 activates it By similarity.
Subunit structure	Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from direct assay Ref.2. Source: CGD G2/M transition of mitotic cell cycle Inferred from genetic interaction Ref.2. Source: CGD cell division Inferred from electronic annotation. Source: UniProtKB-KW hyphal growth Inferred from mutant phenotype PubMed 16710830. Source: CGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell cycle Inferred from genetic interaction Ref.1. Source: CGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from direct assay Ref.2. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Cyclin-dependent kinase 1		PRO_0000085721
Regions
	Domain	7 – 292	286	Protein kinase
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Active site	133	1	Proton acceptor By similarity
	Binding site	37	1	ATP By similarity
Amino acid modifications
	Modified residue	17	1	Phosphothreonine By similarity
	Modified residue	18	1	Phosphotyrosine By similarity
	Modified residue	166	1	Phosphothreonine; by CAK By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43063 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2B91EABE5E5C028B	FASTA	317	36,647
	10 20 30 40 50 60 MVELSDYQRQ EKVGEGTYGV VYKALDTKHN NRVVALKKIR LESEDEGVPS TAIREISLLK 70 80 90 100 110 120 EMKDDNIVRL YDIIHSDSHK LYLVFEFLDL DLKKYMESIP QGVGLGANMI KRFMNQLIRG 130 140 150 160 170 180 IKHCHSHRVL HRDLKPQNLL IDKEGNLKLA DFGLARAFGV PLRAYTHEVV TLWYRAPEIL 190 200 210 220 230 240 LGGKQYSTGV DMWSVGCIFA EMCNRKPLFP GDSEIDEIFR IFRILGTPNE EIWPDVNYLP 250 260 270 280 290 300 DFKSSFPQWK KKPLSEAVPS LDANGIDLLD QMLVYDPSRR ISAKRALIHP YFNDNDDRDH 310 NNYNEDNIGI DKHQNMQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and analysis of CDC28 and cyclin homologues from the human fungal pathogen Candida albicans." Sherlock G., Bahman A.M., Mahal A., Shieh J.C., Ferreira M., Rosamond J. Mol. Gen. Genet. 245:716-723(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SGY126.
[2]	"Candida albicans CDK1 and CYB1: cDNA homologues of the cdc2/CDC28 and cdc13/CLB1/CLB2 cell cycle control genes." Damagnez V., Cottarel G. Gene 172:137-141(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80034 Genomic DNA. Translation: CAA56338.1. U40405 mRNA. Translation: AAC49450.1. AACQ01000125 Genomic DNA. Translation: EAK94372.1. AACQ01000124 Genomic DNA. Translation: EAK94417.1.
PIR	JC4827.
RefSeq	XP_713486.1. XM_708393.1. XP_713525.1. XM_708432.1.
3D structure databases
ProteinModelPortal	P43063.
SMR	P43063. Positions 1-294.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-497N.
MINT	MINT-7295535.
STRING	5476.CAL0002090.
Proteomic databases
PRIDE	P43063.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3644820. 3644838.
KEGG	cal:CaO19.11337. cal:CaO19.3856.
Organism-specific databases
CGD	CAL0002090. CDC28.
Phylogenomic databases
eggNOG	COG0515.
KO	K04563.
Enzyme and pathway databases
BRENDA	2.7.11.22. 1096.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK1_CANAL

Accession

Primary (citable) accession number: P43063
Secondary accession number(s): Q59V96

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program