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Protein

Cyclin-dependent kinase 1

Gene

CDC28

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclin-dependent kinase essential for the completion of the start, the controlling event, in the cell cycle. Plays a role in the expression of morphology-related transcription factors, and especially hyphae-specific genes. Binds distinct cyclin subunits as cells progress through the division cycle or flamentous growth. The CDC28-CLB2 complex regulates cytokinesis partly by phosphorylating the actomyosin ring component IQG1. The CDC28-CLN3 complex phosphorylates SLA1 which regulates cortical actin patch dynamics. The CDC28-CCN1 complex phosphorylates CDC11 and SEC2 upon induction of filamentous growth. The CDC28-HGC1 complex also phosphorylates SEC2 and maintains CDC11 phosphorylation throughout hyphal growth. Moreover, the CDC28-HGC1 complex phosphorylates and prevents RGA2 from localizing to hyphal tips, leading to localized CDC42 activation for hyphal extension. Finally, CDC28-HGC1 phosphorylation of EFG1 represses cell separation genes during hyphal growth. Additional substrates for CDC28 are RFA2 in G1-phase; MOB2, which is required for the maintenance of polarisome components and for inhibition of cell separation in hyphae; and GIN4 to regulate its association to SEP7 and subsequent septin ring assembly.14 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation at Thr-17 or Tyr-18 inactivates the enzyme, while phosphorylation at Thr-166 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATPPROSITE-ProRule annotation1
Active sitei133Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 21ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: CGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: CGD
  • hyphal growth Source: CGD
  • protein phosphorylation Source: CGD
  • regulation of cell cycle Source: CGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22 1096

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1 (EC:2.7.11.22)
Short name:
CDK1
Alternative name(s):
Cell division control protein 28
Cell division protein kinase 2
Gene namesi
Name:CDC28
Synonyms:CDK1
Ordered Locus Names:CAALFM_CR06050WA
ORF Names:CaO19.11337, CaO19.3856
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome R

Organism-specific databases

CGDiCAL0000191263 CDC28

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857211 – 317Cyclin-dependent kinase 1Add BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphothreonineBy similarity1
Modified residuei18Phosphotyrosine; by SWE12 Publications1
Modified residuei166Phosphothreonine; by CAKBy similarity1

Post-translational modificationi

Phosphorylated at Tyr-18 by SWE1 in a cell cycle-dependent manner. Yeast-form and hyphal cells display similar dynamics of phosphorylation and dephosphorylation of Tyr-18. Tyr-18 phosphorylation leads to inhibition of CDC28 kinase activity.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP43063

PTM databases

iPTMnetiP43063

Expressioni

Inductioni

Expression is increased during exponential growth and repressed by the antifungal drug plagiochin E (PLE).2 Publications

Interactioni

Subunit structurei

Forms several complexes with cyclins CCN1, CLB2, CLN3, and HGC1. The CDC28-CCN1 complex associates with septin CDC11 upon hyphal induction. Interacts with IQG1, RFA2, and HSP90.6 Publications

Protein-protein interaction databases

BioGridi1227914, 3 interactors
DIPiDIP-497N
IntActiP43063, 3 interactors
MINTiP43063

Structurei

3D structure databases

ProteinModelPortaliP43063
SMRiP43063
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 292Protein kinasePROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Phylogenomic databases

InParanoidiP43063
KOiK04563
OMAiEMMLVYD
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P43063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVELSDYQRQ EKVGEGTYGV VYKALDTKHN NRVVALKKIR LESEDEGVPS
60 70 80 90 100
TAIREISLLK EMKDDNIVRL YDIIHSDSHK LYLVFEFLDL DLKKYMESIP
110 120 130 140 150
QGVGLGANMI KRFMNQLIRG IKHCHSHRVL HRDLKPQNLL IDKEGNLKLA
160 170 180 190 200
DFGLARAFGV PLRAYTHEVV TLWYRAPEIL LGGKQYSTGV DMWSVGCIFA
210 220 230 240 250
EMCNRKPLFP GDSEIDEIFR IFRILGTPNE EIWPDVNYLP DFKSSFPQWK
260 270 280 290 300
KKPLSEAVPS LDANGIDLLD QMLVYDPSRR ISAKRALIHP YFNDNDDRDH
310
NNYNEDNIGI DKHQNMQ
Length:317
Mass (Da):36,647
Last modified:November 1, 1995 - v1
Checksum:i2B91EABE5E5C028B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80034 Genomic DNA Translation: CAA56338.1
U40405 mRNA Translation: AAC49450.1
CP017630 Genomic DNA Translation: AOW31316.1
PIRiJC4827
RefSeqiXP_713486.1, XM_708393.1

Genome annotation databases

EnsemblFungiiAOW31316; AOW31316; CAALFM_CR06050WA
GeneIDi3644838
KEGGical:CAALFM_CR06050WA

Similar proteinsi

Entry informationi

Entry nameiCDK1_CANAL
AccessioniPrimary (citable) accession number: P43063
Secondary accession number(s): A0A1D8PT63, Q59V96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health