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P43063

- CDK1_CANAL

UniProt

P43063 - CDK1_CANAL

Protein

Cyclin-dependent kinase 1

Gene

CDC28

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Cyclin-dependent kinase essential for the completion of the start, the controlling event, in the cell cycle. Plays a role in the expression of morphology-related transcription factors, and especially hyphae-specific genes. Binds distinct cyclin subunits as cells progress through the division cycle or flamentous growth. The CDC28-CLB2 complex regulates cytokinesis partly by phosphorylating the actomyosin ring component IQG1. The CDC28-CLN3 complex phosphorylates SLA1 which regulates cortical actin patch dynamics. The CDC28-CCN1 complex phosphorylates CDC11 and SEC2 upon induction of filamentous growth. The CDC28-HGC1 complex also phosphorylates SEC2 and maintains CDC11 phosphorylation throughout hyphal growth. Moreover, the CDC28-HGC1 complex phosphorylates and prevents RGA2 from localizing to hyphal tips, leading to localized CDC42 activation for hyphal extension. Finaly CDC28-HGC1 phosphorylation of EFG1 represses cell separation genes during hyphal growth. Additional substrates for CDC28 are RFA2 in G1-phase; MOB2, which is required for the maintenance of polarisome components and for inhibition of cell separation in hyphae; and GIN4 to regulate its association to SEP7 and subsequent septin ring assembly.14 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Phosphorylation at Thr-17 or Tyr-18 inactivates the enzyme, while phosphorylation at Thr-166 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371ATPPROSITE-ProRule annotation
    Active sitei133 – 1331Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 219ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein kinase activating kinase activity Source: CGD
    3. cyclin-dependent protein serine/threonine kinase activity Source: CGD
    4. eukaryotic translation initiation factor 2alpha kinase activity Source: CGD
    5. protein kinase activity Source: CGD
    6. protein serine/threonine kinase activity Source: CGD

    GO - Biological processi

    1. cellular amino acid biosynthetic process Source: CGD
    2. cellular response to drug Source: CGD
    3. cellular response to N-acetyl-D-glucosamine Source: CGD
    4. cellular response to neutral pH Source: CGD
    5. filamentous growth Source: CGD
    6. filamentous growth of a population of unicellular organisms Source: CGD
    7. filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
    8. filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    9. filamentous growth of a population of unicellular organisms in response to neutral pH Source: CGD
    10. G1/S transition of mitotic cell cycle Source: CGD
    11. G2/M transition of mitotic cell cycle Source: CGD
    12. hyphal growth Source: CGD
    13. mitotic nuclear division Source: UniProtKB-KW
    14. phosphate-containing compound metabolic process Source: CGD
    15. positive regulation of filamentous growth of a population of unicellular organisms in response to chemical stimulus Source: CGD
    16. positive regulation of protein kinase activity Source: GOC
    17. protein phosphorylation Source: CGD
    18. regulation of cell cycle Source: CGD
    19. regulation of cyclin-dependent protein serine/threonine kinase activity Source: CGD
    20. single-species biofilm formation on inanimate substrate Source: CGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 1096.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 28
    Cell division protein kinase 2
    Gene namesi
    Name:CDC28
    Synonyms:CDK1
    ORF Names:CaO19.11337, CaO19.3856
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0002090. CDC28.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: CGD
    2. protein kinase CK2 complex Source: CGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Cyclin-dependent kinase 1PRO_0000085721Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei17 – 171PhosphothreonineBy similarity
    Modified residuei18 – 181Phosphotyrosine; by SWE12 Publications
    Modified residuei166 – 1661Phosphothreonine; by CAKBy similarity

    Post-translational modificationi

    Phosphorylated at Tyr-18 by SWE1 in a cell cycle-dependent manner. Yeast-form and hyphal cells display similar dynamics of phosphorylation and dephosphorylation of Tyr-18. Tyr-18 phosphorylation leads to inhibition of CDC28 kinase activity.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP43063.

    Expressioni

    Inductioni

    Expression is increased during exponential growth and repressed by the antifungal drug plagiochin E (PLE).2 Publications

    Interactioni

    Subunit structurei

    Forms several complexes with cyclins CCN1, CLB2, CLN3, and HGC1. The CDC28-CCN1 complex associates with septin CDC11 upon hyphal induction. Interacts with IQG1, RFA2, and HSP90.6 Publications

    Protein-protein interaction databases

    BioGridi1227932. 3 interactions.
    DIPiDIP-497N.
    IntActiP43063. 3 interactions.
    MINTiMINT-7295535.
    STRINGi5476.CAL0002090.

    Structurei

    3D structure databases

    ProteinModelPortaliP43063.
    SMRiP43063. Positions 1-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 292286Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK04563.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P43063-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVELSDYQRQ EKVGEGTYGV VYKALDTKHN NRVVALKKIR LESEDEGVPS    50
    TAIREISLLK EMKDDNIVRL YDIIHSDSHK LYLVFEFLDL DLKKYMESIP 100
    QGVGLGANMI KRFMNQLIRG IKHCHSHRVL HRDLKPQNLL IDKEGNLKLA 150
    DFGLARAFGV PLRAYTHEVV TLWYRAPEIL LGGKQYSTGV DMWSVGCIFA 200
    EMCNRKPLFP GDSEIDEIFR IFRILGTPNE EIWPDVNYLP DFKSSFPQWK 250
    KKPLSEAVPS LDANGIDLLD QMLVYDPSRR ISAKRALIHP YFNDNDDRDH 300
    NNYNEDNIGI DKHQNMQ 317
    Length:317
    Mass (Da):36,647
    Last modified:November 1, 1995 - v1
    Checksum:i2B91EABE5E5C028B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80034 Genomic DNA. Translation: CAA56338.1.
    U40405 mRNA. Translation: AAC49450.1.
    AACQ01000125 Genomic DNA. Translation: EAK94372.1.
    AACQ01000124 Genomic DNA. Translation: EAK94417.1.
    PIRiJC4827.
    RefSeqiXP_713486.1. XM_708393.1.
    XP_713525.1. XM_708432.1.

    Genome annotation databases

    GeneIDi3644820.
    3644838.
    KEGGical:CaO19.11337.
    cal:CaO19.3856.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80034 Genomic DNA. Translation: CAA56338.1 .
    U40405 mRNA. Translation: AAC49450.1 .
    AACQ01000125 Genomic DNA. Translation: EAK94372.1 .
    AACQ01000124 Genomic DNA. Translation: EAK94417.1 .
    PIRi JC4827.
    RefSeqi XP_713486.1. XM_708393.1.
    XP_713525.1. XM_708432.1.

    3D structure databases

    ProteinModelPortali P43063.
    SMRi P43063. Positions 1-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1227932. 3 interactions.
    DIPi DIP-497N.
    IntActi P43063. 3 interactions.
    MINTi MINT-7295535.
    STRINGi 5476.CAL0002090.

    Proteomic databases

    PRIDEi P43063.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3644820.
    3644838.
    KEGGi cal:CaO19.11337.
    cal:CaO19.3856.

    Organism-specific databases

    CGDi CAL0002090. CDC28.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K04563.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 1096.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and analysis of CDC28 and cyclin homologues from the human fungal pathogen Candida albicans."
      Sherlock G., Bahman A.M., Mahal A., Shieh J.C., Ferreira M., Rosamond J.
      Mol. Gen. Genet. 245:716-723(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SGY126.
    2. "Candida albicans CDK1 and CYB1: cDNA homologues of the cdc2/CDC28 and cdc13/CLB1/CLB2 cell cycle control genes."
      Damagnez V., Cottarel G.
      Gene 172:137-141(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    4. "Hyphal elongation is regulated independently of cell cycle in Candida albicans."
      Hazan I., Sepulveda-Becerra M., Liu H.
      Mol. Biol. Cell 13:134-145(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-18, FUNCTION.
    5. "Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis."
      Zheng X., Wang Y., Wang Y.
      EMBO J. 23:1845-1856(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HGC1.
    6. "Candida albicans protein kinase CaHsl1p regulates cell elongation and virulence."
      Umeyama T., Kaneko A., Nagai Y., Hanaoka N., Tanabe K., Takano Y., Niimi M., Uehara Y.
      Mol. Microbiol. 55:381-395(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-18 BY SWE1, FUNCTION.
    7. "Repression of CDC28 reduces the expression of the morphology-related transcription factors, Efg1p, Nrg1p, Rbf1p, Rim101p, Fkh2p and Tec1p and induces cell elongation in Candida albicans."
      Umeyama T., Kaneko A., Niimi M., Uehara Y.
      Yeast 23:537-552(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Phosphorylation of Rga2, a Cdc42 GAP, by CDK/Hgc1 is crucial for Candida albicans hyphal growth."
      Zheng X.D., Lee R.T., Wang Y.M., Lin Q.S., Wang Y.
      EMBO J. 26:3760-3769(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF RGA2.
    9. "Cyclin-dependent kinases control septin phosphorylation in Candida albicans hyphal development."
      Sinha I., Wang Y.M., Philp R., Li C.R., Yap W.H., Wang Y.
      Dev. Cell 13:421-432(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HGC1; CCN1 AND CDC11, FUNCTION IN PHOSPHORYLATION OF CDC11.
    10. "Defining Candida albicans stationary phase by cellular and DNA replication, gene expression and regulation."
      Uppuluri P., Chaffin W.L.
      Mol. Microbiol. 64:1572-1586(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    11. "The IQGAP Iqg1 is a regulatory target of CDK for cytokinesis in Candida albicans."
      Li C.R., Wang Y.M., Wang Y.
      EMBO J. 27:2998-3010(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLB2 AND IQG1, FUNCTION IN PHOSPHORYLATION OF IQG1.
    12. "Hyphal chain formation in Candida albicans: Cdc28-Hgc1 phosphorylation of Efg1 represses cell separation genes."
      Wang A., Raniga P.P., Lane S., Lu Y., Liu H.
      Mol. Cell. Biol. 29:4406-4416(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF EFG1.
    13. "Plagiochin E, an antifungal active macrocyclic bis(bibenzyl), induced apoptosis in Candida albicans through a metacaspase-dependent apoptotic pathway."
      Wu X.Z., Chang W.Q., Cheng A.X., Sun L.M., Lou H.X.
      Biochim. Biophys. Acta 1800:439-447(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    14. "Hyphal growth in Candida albicans requires the phosphorylation of Sec2 by the Cdc28-Ccn1/Hgc1 kinase."
      Bishop A., Lane R., Beniston R., Chapa-y-Lazo B., Smythe C., Sudbery P.
      EMBO J. 29:2930-2942(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF SEC2.
    15. "CDK-dependent phosphorylation of Mob2 is essential for hyphal development in Candida albicans."
      Gutierrez-Escribano P., Gonzalez-Novo A., Suarez M.B., Li C.R., Wang Y., de Aldana C.R., Correa-Bordes J.
      Mol. Biol. Cell 22:2458-2469(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF MOB2.
    16. "CDK regulates septin organization through cell-cycle-dependent phosphorylation of the Nim1-related kinase Gin4."
      Li C.R., Yong J.Y., Wang Y.M., Wang Y.
      J. Cell Sci. 125:2533-2543(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF GIN4.
    17. "Cdc28 provides a molecular link between Hsp90, morphogenesis, and cell cycle progression in Candida albicans."
      Senn H., Shapiro R.S., Cowen L.E.
      Mol. Biol. Cell 23:268-283(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HSP90.
    18. "Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth."
      Zeng G., Wang Y.M., Wang Y.
      Mol. Biol. Cell 23:3485-3497(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CLN3, FUNCTION IN PHOSPHORYLATION OF SLA1.
    19. "Rfa2 is specifically dephosphorylated by Pph3 in Candida albicans."
      Wang H., Gao J., Wong A.H., Hu K., Li W., Wang Y., Sang J.
      Biochem. J. 449:673-681(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RFA2, FUNCTION IN PHOSPHORYLATION OF RFA2.

    Entry informationi

    Entry nameiCDK1_CANAL
    AccessioniPrimary (citable) accession number: P43063
    Secondary accession number(s): Q59V96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3