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Protein

Platelet-activating factor acetylhydrolase IB subunit alpha

Gene

PAFAH1B1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing (By similarity). Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position. Required for pronuclear migration during fertilization.UniRule annotation1 Publication

GO - Molecular functioni

  • dynactin binding Source: BHF-UCL
  • dynein binding Source: BHF-UCL
  • heparin binding Source: BHF-UCL
  • microtubule binding Source: MGI
  • phospholipase binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Differentiation, Lipid degradation, Lipid metabolism, Mitosis, Neurogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit alphaUniRule annotation
Alternative name(s):
Lissencephaly-1 proteinUniRule annotation
Short name:
LIS-1UniRule annotation
PAF acetylhydrolase 45 kDa subunitUniRule annotation
Short name:
PAF-AH 45 kDa subunitUniRule annotation
PAF-AH alphaUniRule annotation
Short name:
PAFAH alphaUniRule annotation
Gene namesi
Name:PAFAH1B1UniRule annotation
Synonyms:LIS1UniRule annotation, PAFAHA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytoskeleton UniRule annotation1 Publication
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation1 Publication
  • Cytoplasmcytoskeletonspindle UniRule annotation
  • Nucleus membrane UniRule annotation

  • Note: Localizes to the plus end of microtubules and to the centrosome. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes (By similarity). May localize to the nuclear membrane.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Platelet-activating factor acetylhydrolase IB subunit alphaPRO_0000051060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP43033.
PRIDEiP43033.

Expressioni

Developmental stagei

Expression increases during fertilization of the oocyte.1 Publication

Interactioni

Subunit structurei

Can self-associate. Interacts with DCX, IQGAP1, KATNB1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling (By similarity). Interacts with dynein, dynactin and NDE1. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Interacts with ASUN (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nde1Q9ES392EBI-1007886,EBI-1007897From a different organism.
Pafah1b2O352642EBI-1007886,EBI-915500From a different organism.

GO - Molecular functioni

  • dynactin binding Source: BHF-UCL
  • dynein binding Source: BHF-UCL
  • microtubule binding Source: MGI
  • phospholipase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi159754. 2 interactions.
IntActiP43033. 3 interactions.
STRINGi9913.ENSBTAP00000052656.

Structurei

3D structure databases

ProteinModelPortaliP43033.
SMRiP43033. Positions 1-79, 92-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 3933LisHUniRule annotationAdd
BLAST
Repeati106 – 14742WD 1Add
BLAST
Repeati148 – 18740WD 2Add
BLAST
Repeati190 – 22940WD 3Add
BLAST
Repeati232 – 27140WD 4Add
BLAST
Repeati274 – 33360WD 5Add
BLAST
Repeati336 – 37742WD 6Add
BLAST
Repeati378 – 41033WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 102102Interaction with NDEL1UniRule annotationAdd
BLAST
Regioni1 – 6666Interaction with NDE1Add
BLAST
Regioni1 – 3838Required for self-association and interaction with PAFAH1B2 and PAFAH1B3UniRule annotationAdd
BLAST
Regioni83 – 410328Interaction with dynein and dynactinUniRule annotationAdd
BLAST
Regioni367 – 40943Interaction with DCXUniRule annotationAdd
BLAST
Regioni388 – 41023Interaction with NDEL1UniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili56 – 8227UniRule annotationAdd
BLAST

Domaini

Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation

Sequence similaritiesi

Belongs to the WD repeat LIS1/nudF family.UniRule annotation
Contains 1 LisH domain.UniRule annotation
Contains 7 WD repeats.UniRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0295. Eukaryota.
ENOG410XP3K. LUCA.
HOGENOMiHOG000184015.
HOVERGENiHBG006271.
InParanoidiP43033.
KOiK16794.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03141. lis1.
InterProiIPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSQRQRDE LNRAIADYLR SNGYEAAYSV FKKEAELDMN EELDKKYAGL
60 70 80 90 100
LEKKWTSVIR LQKKVMELES KLNEAKEEFT SGGPLGQKRD PKEWIPRPPE
110 120 130 140 150
KYALSGHRSP VTRVIFHPVF SVMVSASEDA TIKVWDYETG DFERTLKGHT
160 170 180 190 200
DSVEDISFDH SGKLLASCSA DMTIKLWDFQ GFECIRTMHG HDHNVSSVAI
210 220 230 240 250
MPNGDHIVSA SRDKTIKMWE VQTGYCVKTF TGHREWVRMV RPNQDGTLIA
260 270 280 290 300
SCSNDQTVRV WVVATKECKA ELREHEHVVE CISWAPESSY SSISEATGSE
310 320 330 340 350
TKKSGKPGPF LLSGSRDKTI KMWDVSTGMC LMTLVGHDNW VRGVLFHSGG
360 370 380 390 400
KFILSCADDK TLRVWDYKNK RCMKTLNAHE HFVTSLDFHK TAPYVVTGSV
410
DQTVKVWECR
Length:410
Mass (Da):46,613
Last modified:January 23, 2007 - v2
Checksum:iFE5C2DF0AB98F3B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30615 mRNA. Translation: BAA06305.1.
RefSeqiNP_777088.1. NM_174663.2.
UniGeneiBt.111460.

Genome annotation databases

GeneIDi282513.
KEGGibta:282513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30615 mRNA. Translation: BAA06305.1.
RefSeqiNP_777088.1. NM_174663.2.
UniGeneiBt.111460.

3D structure databases

ProteinModelPortaliP43033.
SMRiP43033. Positions 1-79, 92-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159754. 2 interactions.
IntActiP43033. 3 interactions.
STRINGi9913.ENSBTAP00000052656.

Proteomic databases

PaxDbiP43033.
PRIDEiP43033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282513.
KEGGibta:282513.

Organism-specific databases

CTDi5048.

Phylogenomic databases

eggNOGiKOG0295. Eukaryota.
ENOG410XP3K. LUCA.
HOGENOMiHOG000184015.
HOVERGENiHBG006271.
InParanoidiP43033.
KOiK16794.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03141. lis1.
InterProiIPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Miller-Dieker lissencephaly gene encodes a subunit of brain platelet-activating factor acetylhydrolase."
    Hattori M., Adachi H., Tsujimoto M., Arai H., Inoue K.
    Nature 370:216-218(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.
  2. "Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE."
    Kitagawa M., Umezu M., Aoki J., Koizumi H., Arai H., Inoue K.
    FEBS Lett. 479:57-62(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAFAH1B2; PAFAH1B3 AND NDE1.
  3. "A role for the lissencephaly gene LIS1 in mitosis and cytoplasmic dynein function."
    Faulkner N.E., Dujardin D.L., Tai C.-Y., Vaughan K.T., O'Connell C.B., Wang Y.-L., Vallee R.B.
    Nat. Cell Biol. 2:784-791(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYNEIN AND DYNACTIN.
  4. "LIS1 association with dynactin is required for nuclear motility and genomic union in the fertilized mammalian oocyte."
    Payne C., St John J.C., Ramalho-Santos J., Schatten G.
    Cell Motil. Cytoskeleton 56:245-251(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DYNACTIN, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiLIS1_BOVIN
AccessioniPrimary (citable) accession number: P43033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.