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P43021 (NODAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nodal
Gene names
Name:Nodal
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential for mesoderm formation and axial patterning during embryonic development. Ref.1 Ref.4

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Secreted By similarity.

Developmental stage

Expressed in the node during gastrulation. Expression is first detected in primitive streak-stage embryos at about the time of mesoderm formation. It then becomes highly localized in the node at the anterior of the primitive streak. Ref.1

Sequence similarities

Belongs to the TGF-beta family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Developmental protein
Growth factor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSMAD protein signal transduction

Inferred from direct assay PubMed 9420335. Source: MGI

anatomical structure formation involved in morphogenesis

Inferred from mutant phenotype PubMed 7924997. Source: MGI

anterior/posterior axis specification

Inferred from mutant phenotype PubMed 11311163. Source: MGI

anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 11311163PubMed 11418863PubMed 15004567PubMed 15302604. Source: MGI

axial mesodermal cell fate specification

Inferred from mutant phenotype PubMed 14511481. Source: MGI

brain development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

cell fate commitment

Inferred from mutant phenotype PubMed 12842913. Source: MGI

cell migration

Inferred from mutant phenotype PubMed 15302604. Source: MGI

cell migration involved in gastrulation

Inferred from direct assay PubMed 15004567. Source: MGI

determination of left/right symmetry

Traceable author statement PubMed 9354794. Source: BHF-UCL

digestive system development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

digestive tract morphogenesis

Inferred from mutant phenotype PubMed 11311163. Source: MGI

embryonic cranial skeleton morphogenesis

Inferred from mutant phenotype PubMed 11311163. Source: MGI

embryonic heart tube development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

embryonic pattern specification

Inferred from mutant phenotype PubMed 11311163. Source: MGI

embryonic placenta development

Inferred from mutant phenotype PubMed 11456449. Source: MGI

embryonic process involved in female pregnancy

Inferred from mutant phenotype PubMed 11456449. Source: MGI

endoderm development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

endodermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification

Inferred from mutant phenotype PubMed 14511481. Source: MGI

floor plate morphogenesis

Inferred from mutant phenotype PubMed 11311163. Source: MGI

formation of anatomical boundary

Inferred from mutant phenotype PubMed 11311163. Source: MGI

gastrulation

Inferred from mutant phenotype PubMed 11311163PubMed 14511481. Source: MGI

gastrulation with mouth forming second

Inferred from mutant phenotype PubMed 14607953. Source: MGI

growth

Inferred from electronic annotation. Source: InterPro

heart development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

heart looping

Inferred from genetic interaction PubMed 16496285. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 11311163PubMed 15485907. Source: MGI

inhibition of neuroepithelial cell differentiation

Inferred from mutant phenotype PubMed 16678814. Source: MGI

left lung morphogenesis

Inferred from mutant phenotype PubMed 11311163. Source: MGI

liver development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

lung development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

maternal placenta development

Inferred from mutant phenotype PubMed 22378764. Source: MGI

maternal process involved in parturition

Inferred from mutant phenotype PubMed 22378764. Source: MGI

mesendoderm development

Inferred from mutant phenotype PubMed 11311163PubMed 14511481. Source: MGI

mesoderm formation

Traceable author statement Ref.1. Source: BHF-UCL

negative regulation of androgen receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell differentiation

Inferred from direct assay PubMed 11456449. Source: MGI

negative regulation of chorionic trophoblast cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11456449. Source: MGI

negative regulation of trophoblast cell migration

Inferred from electronic annotation. Source: Ensembl

nervous system development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

neural fold formation

Inferred from mutant phenotype PubMed 11311163. Source: MGI

nodal signaling pathway

Inferred from mutant phenotype PubMed 12052855. Source: UniProtKB

nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry

Inferred from mutant phenotype PubMed 17507406. Source: BHF-UCL

polarity specification of proximal/distal axis

Inferred from mutant phenotype PubMed 14511481. Source: MGI

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of SMAD protein import into nucleus

Inferred from electronic annotation. Source: Ensembl

positive regulation of activin receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from mutant phenotype PubMed 21116837. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 15004567. Source: MGI

positive regulation of cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of pathway-restricted SMAD protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 17507406. Source: BHF-UCL

positive regulation vascular endothelial growth factor production

Inferred from electronic annotation. Source: Ensembl

primitive streak formation

Inferred from genetic interaction PubMed 21445260. Source: MGI

regulation of gastrulation

Inferred from genetic interaction PubMed 20040491. Source: MGI

regulation of signal transduction

Inferred from direct assay PubMed 17936261. Source: MGI

stem cell maintenance

Inferred from mutant phenotype PubMed 16678814. Source: MGI

tissue morphogenesis

Inferred from mutant phenotype PubMed 11311163. Source: MGI

transforming growth factor beta receptor signaling pathway

Inferred from sequence alignment Ref.1. Source: MGI

transforming growth factor beta receptor signaling pathway involved in primitive streak formation

Inferred from mutant phenotype PubMed 14511481. Source: MGI

trophectodermal cell differentiation

Inferred from direct assay PubMed 11456449. Source: MGI

trophectodermal cellular morphogenesis

Inferred from mutant phenotype PubMed 11456449. Source: MGI

vasculature development

Inferred from mutant phenotype PubMed 11311163. Source: MGI

   Cellular_componentextracellular region

Inferred from direct assay PubMed 12052855. Source: UniProtKB

extracellular space

Traceable author statement Ref.1. Source: BHF-UCL

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 12052855PubMed 15062104. Source: UniProtKB

receptor agonist activity

Inferred from direct assay PubMed 12052855. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 244218 Potential
PRO_0000034000
Chain245 – 354110Nodal
PRO_0000034001

Amino acid modifications

Glycosylation731N-linked (GlcNAc...) Potential
Disulfide bond254 ↔ 320 By similarity
Disulfide bond283 ↔ 351 By similarity
Disulfide bond287 ↔ 353 By similarity
Disulfide bond319Interchain By similarity

Experimental info

Sequence conflict93 – 942EL → DV in CAA49914. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P43021 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 40ECFC6785BB5826

FASTA35440,476
        10         20         30         40         50         60 
MSAHSLRILL LQACWALLHP RAPTAAALPL WTRGQPSSPS PLAYMLSLYR DPLPRADIIR 

        70         80         90        100        110        120 
SLQAQDVDVT GQNWTFTFDF SFLSQEEDLV WAELRLQLPG PMDIPTEGPL TIDIFHQAKG 

       130        140        150        160        170        180 
DPERDPADCL ERIWMETFTV IPSQVTFASG STVLEVTKPL SKWLKDPRAL EKQVSSRAEK 

       190        200        210        220        230        240 
CWHQPYTPPV PVASTNVLML YSNRPQEQRQ LGGATLLWEA ESSWRAQEGQ LSVERGGWGR 

       250        260        270        280        290        300 
RQRRHHLPDR SQLCRRVKFQ VDFNLIGWGS WIIYPKQYNA YRCEGECPNP VGEEFHPTNH 

       310        320        330        340        350 
AYIQSLLKRY QPHRVPSTCC APVKTKPLSM LYVDNGRVLL EHHKDMIVEE CGCL 

« Hide

References

« Hide 'large scale' references
[1]"Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation."
Zhou X., Sasaki H., Lowe L., Hogan B.L., Kuehn M.R.
Nature 361:543-547(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation."
Jones C.M., Kuehn M.R., Hogan B.L.M., Smith J.C., Wright C.V.E.
Development 121:3651-3662(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70514 mRNA. Translation: CAA49914.1.
AK049290 mRNA. Translation: BAC33661.1.
AK082823 mRNA. Translation: BAC38637.1.
CH466553 Genomic DNA. Translation: EDL32144.1.
CCDSCCDS23878.1.
PIRS29718.
RefSeqNP_038639.2. NM_013611.4.
UniGeneMm.57195.

3D structure databases

ProteinModelPortalP43021.
SMRP43021. Positions 253-353.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201801. 2 interactions.
STRING10090.ENSMUSP00000039653.

Proteomic databases

PRIDEP43021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049339; ENSMUSP00000039653; ENSMUSG00000037171.
GeneID18119.
KEGGmmu:18119.
UCSCuc007ffy.2. mouse.

Organism-specific databases

CTD4838.
MGIMGI:97359. Nodal.

Phylogenomic databases

eggNOGNOG278576.
GeneTreeENSGT00740000114888.
HOGENOMHOG000113815.
HOVERGENHBG108195.
InParanoidQ8BH87.
KOK04666.
OMAHRVPSTC.
OrthoDBEOG7DFXD1.
TreeFamTF316134.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

ArrayExpressP43021.
BgeeP43021.
CleanExMM_NODAL.
GenevestigatorP43021.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERPTHR11848. PTHR11848. 1 hit.
PfamPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio293328.
PROP43021.
SOURCESearch...

Entry information

Entry nameNODAL_MOUSE
AccessionPrimary (citable) accession number: P43021
Secondary accession number(s): Q8BH87
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot