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P43021

- NODAL_MOUSE

UniProt

P43021 - NODAL_MOUSE

Protein

Nodal

Gene

Nodal

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Essential for mesoderm formation and axial patterning during embryonic development.2 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. receptor agonist activity Source: UniProtKB

    GO - Biological processi

    1. anatomical structure formation involved in morphogenesis Source: MGI
    2. anterior/posterior axis specification Source: MGI
    3. anterior/posterior pattern specification Source: MGI
    4. axial mesodermal cell fate specification Source: MGI
    5. brain development Source: MGI
    6. cell fate commitment Source: MGI
    7. cell migration Source: MGI
    8. cell migration involved in gastrulation Source: MGI
    9. determination of left/right symmetry Source: BHF-UCL
    10. digestive system development Source: MGI
    11. digestive tract morphogenesis Source: MGI
    12. embryonic cranial skeleton morphogenesis Source: MGI
    13. embryonic heart tube development Source: MGI
    14. embryonic pattern specification Source: MGI
    15. embryonic placenta development Source: MGI
    16. embryonic process involved in female pregnancy Source: MGI
    17. endodermal cell differentiation Source: Ensembl
    18. endoderm development Source: MGI
    19. epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification Source: MGI
    20. floor plate morphogenesis Source: MGI
    21. formation of anatomical boundary Source: MGI
    22. gastrulation Source: MGI
    23. gastrulation with mouth forming second Source: MGI
    24. growth Source: InterPro
    25. heart development Source: MGI
    26. heart looping Source: MGI
    27. inhibition of neuroepithelial cell differentiation Source: MGI
    28. in utero embryonic development Source: MGI
    29. left lung morphogenesis Source: MGI
    30. liver development Source: MGI
    31. lung development Source: MGI
    32. maternal placenta development Source: MGI
    33. maternal process involved in parturition Source: MGI
    34. mesendoderm development Source: MGI
    35. mesoderm formation Source: BHF-UCL
    36. negative regulation of androgen receptor signaling pathway Source: Ensembl
    37. negative regulation of cell differentiation Source: MGI
    38. negative regulation of chorionic trophoblast cell proliferation Source: Ensembl
    39. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    40. negative regulation of trophoblast cell migration Source: Ensembl
    41. nervous system development Source: MGI
    42. neural fold formation Source: MGI
    43. nodal signaling pathway Source: UniProtKB
    44. nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry Source: BHF-UCL
    45. polarity specification of proximal/distal axis Source: MGI
    46. positive regulation of activin receptor signaling pathway Source: Ensembl
    47. positive regulation of angiogenesis Source: BHF-UCL
    48. positive regulation of cell-cell adhesion Source: Ensembl
    49. positive regulation of cell proliferation Source: MGI
    50. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    51. positive regulation of epithelial cell proliferation Source: Ensembl
    52. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    53. positive regulation of pathway-restricted SMAD protein phosphorylation Source: Ensembl
    54. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    55. positive regulation of SMAD protein import into nucleus Source: Ensembl
    56. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    57. positive regulation vascular endothelial growth factor production Source: Ensembl
    58. primitive streak formation Source: MGI
    59. regulation of gastrulation Source: MGI
    60. regulation of signal transduction Source: MGI
    61. SMAD protein signal transduction Source: MGI
    62. stem cell maintenance Source: MGI
    63. tissue morphogenesis Source: MGI
    64. transforming growth factor beta receptor signaling pathway Source: MGI
    65. transforming growth factor beta receptor signaling pathway involved in primitive streak formation Source: MGI
    66. trophectodermal cell differentiation Source: MGI
    67. trophectodermal cellular morphogenesis Source: MGI
    68. vasculature development Source: MGI

    Keywords - Molecular functioni

    Cytokine, Developmental protein, Growth factor

    Enzyme and pathway databases

    ReactomeiREACT_199125. Regulation of signaling by NODAL.
    REACT_220645. Signaling by NODAL.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nodal
    Gene namesi
    Name:Nodal
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:97359. Nodal.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: BHF-UCL

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Propeptidei27 – 244218Sequence AnalysisPRO_0000034000Add
    BLAST
    Chaini245 – 354110NodalPRO_0000034001Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi73 – 731N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi254 ↔ 320By similarity
    Disulfide bondi283 ↔ 351By similarity
    Disulfide bondi287 ↔ 353By similarity
    Disulfide bondi319 – 319InterchainBy similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP43021.

    Expressioni

    Developmental stagei

    Expressed in the node during gastrulation. Expression is first detected in primitive streak-stage embryos at about the time of mesoderm formation. It then becomes highly localized in the node at the anterior of the primitive streak.1 Publication

    Gene expression databases

    ArrayExpressiP43021.
    BgeeiP43021.
    CleanExiMM_NODAL.
    GenevestigatoriP43021.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    BioGridi201801. 2 interactions.
    STRINGi10090.ENSMUSP00000039653.

    Structurei

    3D structure databases

    ProteinModelPortaliP43021.
    SMRiP43021. Positions 253-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TGF-beta family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG278576.
    GeneTreeiENSGT00740000114888.
    HOGENOMiHOG000113815.
    HOVERGENiHBG108195.
    InParanoidiQ8BH87.
    KOiK04666.
    OMAiHRVPSTC.
    OrthoDBiEOG7DFXD1.
    TreeFamiTF316134.

    Family and domain databases

    Gene3Di2.10.90.10. 1 hit.
    InterProiIPR029034. Cystine-knot_cytokine.
    IPR001839. TGF-b_C.
    IPR001111. TGF-b_N.
    IPR015615. TGF-beta-rel.
    IPR017948. TGFb_CS.
    [Graphical view]
    PANTHERiPTHR11848. PTHR11848. 1 hit.
    PfamiPF00019. TGF_beta. 1 hit.
    PF00688. TGFb_propeptide. 1 hit.
    [Graphical view]
    SMARTiSM00204. TGFB. 1 hit.
    [Graphical view]
    SUPFAMiSSF57501. SSF57501. 1 hit.
    PROSITEiPS00250. TGF_BETA_1. 1 hit.
    PS51362. TGF_BETA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P43021-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAHSLRILL LQACWALLHP RAPTAAALPL WTRGQPSSPS PLAYMLSLYR    50
    DPLPRADIIR SLQAQDVDVT GQNWTFTFDF SFLSQEEDLV WAELRLQLPG 100
    PMDIPTEGPL TIDIFHQAKG DPERDPADCL ERIWMETFTV IPSQVTFASG 150
    STVLEVTKPL SKWLKDPRAL EKQVSSRAEK CWHQPYTPPV PVASTNVLML 200
    YSNRPQEQRQ LGGATLLWEA ESSWRAQEGQ LSVERGGWGR RQRRHHLPDR 250
    SQLCRRVKFQ VDFNLIGWGS WIIYPKQYNA YRCEGECPNP VGEEFHPTNH 300
    AYIQSLLKRY QPHRVPSTCC APVKTKPLSM LYVDNGRVLL EHHKDMIVEE 350
    CGCL 354
    Length:354
    Mass (Da):40,476
    Last modified:July 27, 2011 - v2
    Checksum:i40ECFC6785BB5826
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 942EL → DV in CAA49914. (PubMed:8429908)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70514 mRNA. Translation: CAA49914.1.
    AK049290 mRNA. Translation: BAC33661.1.
    AK082823 mRNA. Translation: BAC38637.1.
    CH466553 Genomic DNA. Translation: EDL32144.1.
    CCDSiCCDS23878.1.
    PIRiS29718.
    RefSeqiNP_038639.2. NM_013611.4.
    UniGeneiMm.57195.

    Genome annotation databases

    EnsembliENSMUST00000049339; ENSMUSP00000039653; ENSMUSG00000037171.
    GeneIDi18119.
    KEGGimmu:18119.
    UCSCiuc007ffy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70514 mRNA. Translation: CAA49914.1 .
    AK049290 mRNA. Translation: BAC33661.1 .
    AK082823 mRNA. Translation: BAC38637.1 .
    CH466553 Genomic DNA. Translation: EDL32144.1 .
    CCDSi CCDS23878.1.
    PIRi S29718.
    RefSeqi NP_038639.2. NM_013611.4.
    UniGenei Mm.57195.

    3D structure databases

    ProteinModelPortali P43021.
    SMRi P43021. Positions 253-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201801. 2 interactions.
    STRINGi 10090.ENSMUSP00000039653.

    Proteomic databases

    PRIDEi P43021.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049339 ; ENSMUSP00000039653 ; ENSMUSG00000037171 .
    GeneIDi 18119.
    KEGGi mmu:18119.
    UCSCi uc007ffy.2. mouse.

    Organism-specific databases

    CTDi 4838.
    MGIi MGI:97359. Nodal.

    Phylogenomic databases

    eggNOGi NOG278576.
    GeneTreei ENSGT00740000114888.
    HOGENOMi HOG000113815.
    HOVERGENi HBG108195.
    InParanoidi Q8BH87.
    KOi K04666.
    OMAi HRVPSTC.
    OrthoDBi EOG7DFXD1.
    TreeFami TF316134.

    Enzyme and pathway databases

    Reactomei REACT_199125. Regulation of signaling by NODAL.
    REACT_220645. Signaling by NODAL.

    Miscellaneous databases

    NextBioi 293328.
    PROi P43021.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43021.
    Bgeei P43021.
    CleanExi MM_NODAL.
    Genevestigatori P43021.

    Family and domain databases

    Gene3Di 2.10.90.10. 1 hit.
    InterProi IPR029034. Cystine-knot_cytokine.
    IPR001839. TGF-b_C.
    IPR001111. TGF-b_N.
    IPR015615. TGF-beta-rel.
    IPR017948. TGFb_CS.
    [Graphical view ]
    PANTHERi PTHR11848. PTHR11848. 1 hit.
    Pfami PF00019. TGF_beta. 1 hit.
    PF00688. TGFb_propeptide. 1 hit.
    [Graphical view ]
    SMARTi SM00204. TGFB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57501. SSF57501. 1 hit.
    PROSITEi PS00250. TGF_BETA_1. 1 hit.
    PS51362. TGF_BETA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation."
      Zhou X., Sasaki H., Lowe L., Hogan B.L., Kuehn M.R.
      Nature 361:543-547(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Embryo.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation."
      Jones C.M., Kuehn M.R., Hogan B.L.M., Smith J.C., Wright C.V.E.
      Development 121:3651-3662(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNODAL_MOUSE
    AccessioniPrimary (citable) accession number: P43021
    Secondary accession number(s): Q8BH87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3