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Protein

Nodal

Gene

Nodal

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for mesoderm formation and axial patterning during embryonic development.2 Publications

GO - Molecular functioni

GO - Biological processi

  • anatomical structure formation involved in morphogenesis Source: MGI
  • anterior/posterior axis specification Source: MGI
  • anterior/posterior pattern specification Source: MGI
  • axial mesodermal cell fate specification Source: MGI
  • brain development Source: MGI
  • cell fate commitment Source: MGI
  • cell migration Source: MGI
  • cell migration involved in gastrulation Source: MGI
  • determination of left/right symmetry Source: BHF-UCL
  • digestive system development Source: MGI
  • digestive tract morphogenesis Source: MGI
  • embryonic cranial skeleton morphogenesis Source: MGI
  • embryonic heart tube development Source: MGI
  • embryonic pattern specification Source: MGI
  • embryonic placenta development Source: MGI
  • embryonic process involved in female pregnancy Source: MGI
  • endodermal cell differentiation Source: MGI
  • endoderm development Source: MGI
  • epiblast cell-extraembryonic ectoderm cell signaling involved in anterior/posterior axis specification Source: MGI
  • floor plate morphogenesis Source: MGI
  • formation of anatomical boundary Source: MGI
  • gastrulation Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • growth Source: InterPro
  • heart development Source: MGI
  • heart looping Source: MGI
  • inhibition of neuroepithelial cell differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • left lung morphogenesis Source: MGI
  • liver development Source: MGI
  • lung development Source: MGI
  • maternal placenta development Source: MGI
  • maternal process involved in parturition Source: MGI
  • mesendoderm development Source: MGI
  • mesoderm formation Source: BHF-UCL
  • negative regulation of androgen receptor signaling pathway Source: MGI
  • negative regulation of cell development Source: MGI
  • negative regulation of cell differentiation Source: MGI
  • negative regulation of chorionic trophoblast cell proliferation Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • negative regulation of trophoblast cell migration Source: MGI
  • nervous system development Source: MGI
  • neural fold formation Source: MGI
  • nodal signaling pathway Source: UniProtKB
  • nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry Source: BHF-UCL
  • placenta development Source: MGI
  • polarity specification of proximal/distal axis Source: MGI
  • positive regulation of activin receptor signaling pathway Source: MGI
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of cell-cell adhesion Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
  • positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  • positive regulation of SMAD protein import into nucleus Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of vascular endothelial growth factor production Source: MGI
  • primitive streak formation Source: MGI
  • regulation of apoptotic process Source: GO_Central
  • regulation of gastrulation Source: MGI
  • regulation of MAPK cascade Source: GO_Central
  • regulation of signal transduction Source: MGI
  • SMAD protein signal transduction Source: MGI
  • stem cell maintenance Source: MGI
  • tissue morphogenesis Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI
  • transforming growth factor beta receptor signaling pathway involved in primitive streak formation Source: MGI
  • trophectodermal cell differentiation Source: MGI
  • trophectodermal cellular morphogenesis Source: MGI
  • vasculature development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Developmental protein, Growth factor

Enzyme and pathway databases

ReactomeiREACT_304594. Regulation of signaling by NODAL.
REACT_331083. Signaling by NODAL.

Names & Taxonomyi

Protein namesi
Recommended name:
Nodal
Gene namesi
Name:Nodal
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97359. Nodal.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Propeptidei27 – 244218Sequence AnalysisPRO_0000034000Add
BLAST
Chaini245 – 354110NodalPRO_0000034001Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi254 ↔ 320By similarity
Disulfide bondi283 ↔ 351By similarity
Disulfide bondi287 ↔ 353By similarity
Disulfide bondi319 – 319InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP43021.

Expressioni

Developmental stagei

Expressed in the node during gastrulation. Expression is first detected in primitive streak-stage embryos at about the time of mesoderm formation. It then becomes highly localized in the node at the anterior of the primitive streak.1 Publication

Gene expression databases

BgeeiP43021.
CleanExiMM_NODAL.
ExpressionAtlasiP43021. baseline and differential.
GenevestigatoriP43021.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi201801. 2 interactions.
STRINGi10090.ENSMUSP00000039653.

Structurei

3D structure databases

ProteinModelPortaliP43021.
SMRiP43021. Positions 253-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG278576.
GeneTreeiENSGT00740000114888.
HOGENOMiHOG000113815.
HOVERGENiHBG108195.
InParanoidiP43021.
KOiK04666.
OMAiEVDGQNW.
OrthoDBiEOG7DFXD1.
TreeFamiTF316134.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P43021-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHSLRILL LQACWALLHP RAPTAAALPL WTRGQPSSPS PLAYMLSLYR
60 70 80 90 100
DPLPRADIIR SLQAQDVDVT GQNWTFTFDF SFLSQEEDLV WAELRLQLPG
110 120 130 140 150
PMDIPTEGPL TIDIFHQAKG DPERDPADCL ERIWMETFTV IPSQVTFASG
160 170 180 190 200
STVLEVTKPL SKWLKDPRAL EKQVSSRAEK CWHQPYTPPV PVASTNVLML
210 220 230 240 250
YSNRPQEQRQ LGGATLLWEA ESSWRAQEGQ LSVERGGWGR RQRRHHLPDR
260 270 280 290 300
SQLCRRVKFQ VDFNLIGWGS WIIYPKQYNA YRCEGECPNP VGEEFHPTNH
310 320 330 340 350
AYIQSLLKRY QPHRVPSTCC APVKTKPLSM LYVDNGRVLL EHHKDMIVEE

CGCL
Length:354
Mass (Da):40,476
Last modified:July 27, 2011 - v2
Checksum:i40ECFC6785BB5826
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 942EL → DV in CAA49914 (PubMed:8429908).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70514 mRNA. Translation: CAA49914.1.
AK049290 mRNA. Translation: BAC33661.1.
AK082823 mRNA. Translation: BAC38637.1.
CH466553 Genomic DNA. Translation: EDL32144.1.
CCDSiCCDS23878.1.
PIRiS29718.
RefSeqiNP_038639.2. NM_013611.4.
UniGeneiMm.57195.

Genome annotation databases

EnsembliENSMUST00000049339; ENSMUSP00000039653; ENSMUSG00000037171.
GeneIDi18119.
KEGGimmu:18119.
UCSCiuc007ffy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70514 mRNA. Translation: CAA49914.1.
AK049290 mRNA. Translation: BAC33661.1.
AK082823 mRNA. Translation: BAC38637.1.
CH466553 Genomic DNA. Translation: EDL32144.1.
CCDSiCCDS23878.1.
PIRiS29718.
RefSeqiNP_038639.2. NM_013611.4.
UniGeneiMm.57195.

3D structure databases

ProteinModelPortaliP43021.
SMRiP43021. Positions 253-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201801. 2 interactions.
STRINGi10090.ENSMUSP00000039653.

Proteomic databases

PRIDEiP43021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049339; ENSMUSP00000039653; ENSMUSG00000037171.
GeneIDi18119.
KEGGimmu:18119.
UCSCiuc007ffy.2. mouse.

Organism-specific databases

CTDi4838.
MGIiMGI:97359. Nodal.

Phylogenomic databases

eggNOGiNOG278576.
GeneTreeiENSGT00740000114888.
HOGENOMiHOG000113815.
HOVERGENiHBG108195.
InParanoidiP43021.
KOiK04666.
OMAiEVDGQNW.
OrthoDBiEOG7DFXD1.
TreeFamiTF316134.

Enzyme and pathway databases

ReactomeiREACT_304594. Regulation of signaling by NODAL.
REACT_331083. Signaling by NODAL.

Miscellaneous databases

NextBioi293328.
PROiP43021.
SOURCEiSearch...

Gene expression databases

BgeeiP43021.
CleanExiMM_NODAL.
ExpressionAtlasiP43021. baseline and differential.
GenevestigatoriP43021.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation."
    Zhou X., Sasaki H., Lowe L., Hogan B.L., Kuehn M.R.
    Nature 361:543-547(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation."
    Jones C.M., Kuehn M.R., Hogan B.L.M., Smith J.C., Wright C.V.E.
    Development 121:3651-3662(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNODAL_MOUSE
AccessioniPrimary (citable) accession number: P43021
Secondary accession number(s): Q8BH87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: May 27, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.