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P43012 (TOP1_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:HI_1365
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length868 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 868868DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145149

Regions

Domain3 – 148146Toprim
Zinc finger605 – 63632C4-type 1 HAMAP-Rule MF_00952
Zinc finger667 – 69428C4-type 2 HAMAP-Rule MF_00952
Zinc finger716 – 73924C4-type 3 HAMAP-Rule MF_00952
Region198 – 2036Interaction with DNA By similarity

Sites

Active site3251O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding1171Magnesium 1; catalytic By similarity
Metal binding1171Magnesium 2 By similarity
Metal binding1191Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1741Interaction with DNA By similarity
Site1751Interaction with DNA By similarity
Site1781Interaction with DNA By similarity
Site1901Interaction with DNA By similarity
Site3271Interaction with DNA By similarity
Site5131Interaction with DNA By similarity

Experimental info

Sequence conflict3431T → S in AAC23012. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P43012 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: AAD604BDEBC75D20

FASTA86898,144
        10         20         30         40         50         60 
MSKSLVIVES PAKAKTINKY LGSQYVVKSS VGHIRDLPTV GSSTGEKAKP ISTKGMDAEE 

        70         80         90        100        110        120 
KAKIKAEKER NALVKRMGID PYHDWKANYQ ILPGKEKVVS ELKSLAKKAD HIYLATDLDR 

       130        140        150        160        170        180 
EGEAIAWHLR EVIGGNDDRF SRVVFNEITK NAIKQAFEKP EQLNMDRVNA QQTRRFLDRV 

       190        200        210        220        230        240 
VGFMVSPLLW KKVARGLSAG RVQSVAVKLL VEREREIKAF QPEEYWEVAV LTNNQNKQAI 

       250        260        270        280        290        300 
RLDVTDYKGK KFDPKNQKEA QSAVDFLNVS DYVVTDLETK PTSSRPRAPF ITSTLQQTAS 

       310        320        330        340        350        360 
TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALNM ARTYIENHFG AQYLPEKPNF 

       370        380        390        400        410        420 
YSSKENAQEA HEAIRPSDIR ALPESLEGME KDAVRLYDLI WCQFLACQMP PAQYDSSTLT 

       430        440        450        460        470        480 
VTAGDYTLKA KGRILRFDGW TKVLPQIGKN PEDQELPSVT VSEKLALKEV QPTQHFTKPP 

       490        500        510        520        530        540 
ARFTEAALVK ELEKRGIGRP STYAAIISTI QERGYVRTEN RRFYAEKMGE IVTDRLNESF 

       550        560        570        580        590        600 
GELMNYDFTA NMEDTLDKIA SGSVNWKTEL NQFFKDFSSQ LSKAELDELE GGMRPNSLVE 

       610        620        630        640        650        660 
TDIKCPTCGR NMAIRTASTG VFLGCTGYAL PPKERCKTTI NLIPEAELLN VLDESSETKA 

       670        680        690        700        710        720 
LMDRKRCTKC GTAMDSYVID AHRKIHICGN NPNCDGYLIE EGSFKIKGYD GPVVECDKCG 

       730        740        750        760        770        780 
ADMHLKLGRF GKYMGCTNCD NTRKILKNGE VAPPKEEPVH FPELKCEKSD AYFVLRDGAS 

       790        800        810        820        830        840 
GVFMSAHNFP KSRETRPVKI AELVQYRERL PEKLAYLADA PQKDPEENEA IVRFSRKEKK 

       850        860 
QYVTSEKEGK ATKWIVDFTN GKWVERKK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20964 Genomic DNA. Translation: AAC43727.1.
L42023 Genomic DNA. Translation: AAC23012.1.
PIRG64119.
RefSeqNP_439516.1. NC_000907.1.

3D structure databases

ProteinModelPortalP43012.
SMRP43012. Positions 3-598, 748-868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1365.

Proteomic databases

PRIDEP43012.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC23012; AAC23012; HI_1365.
GeneID950025.
KEGGhin:HI1365.
PATRIC20191417. VBIHaeInf48452_1419.

Phylogenomic databases

eggNOGCOG0551.
KOK03168.
OMAMVYKMGR.
OrthoDBEOG6S7XQ9.
ProtClustDBPRK07561.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_HAEIN
AccessionPrimary (citable) accession number: P43012
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names