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P43012

- TOP1_HAEIN

UniProt

P43012 - TOP1_HAEIN

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalyticUniRule annotation
Sitei33 – 331Interaction with DNAUniRule annotation
Metal bindingi117 – 1171Magnesium 1; catalyticUniRule annotation
Metal bindingi117 – 1171Magnesium 2UniRule annotation
Metal bindingi119 – 1191Magnesium 2UniRule annotation
Sitei174 – 1741Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Sitei178 – 1781Interaction with DNAUniRule annotation
Sitei190 – 1901Interaction with DNAUniRule annotation
Active sitei325 – 3251O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei327 – 3271Interaction with DNAUniRule annotation
Sitei513 – 5131Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri605 – 63632C4-type 1Add
BLAST
Zinc fingeri667 – 69428C4-type 2Add
BLAST
Zinc fingeri716 – 73924C4-type 3Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:HI_1365
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 868868DNA topoisomerase 1PRO_0000145149Add
BLAST

Proteomic databases

PRIDEiP43012.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI1365.

Structurei

3D structure databases

ProteinModelPortaliP43012.
SMRiP43012. Positions 3-598, 748-868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 148146ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni198 – 2036Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri605 – 63632C4-type 1Add
BLAST
Zinc fingeri667 – 69428C4-type 2Add
BLAST
Zinc fingeri716 – 73924C4-type 3Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P43012-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKSLVIVES PAKAKTINKY LGSQYVVKSS VGHIRDLPTV GSSTGEKAKP
60 70 80 90 100
ISTKGMDAEE KAKIKAEKER NALVKRMGID PYHDWKANYQ ILPGKEKVVS
110 120 130 140 150
ELKSLAKKAD HIYLATDLDR EGEAIAWHLR EVIGGNDDRF SRVVFNEITK
160 170 180 190 200
NAIKQAFEKP EQLNMDRVNA QQTRRFLDRV VGFMVSPLLW KKVARGLSAG
210 220 230 240 250
RVQSVAVKLL VEREREIKAF QPEEYWEVAV LTNNQNKQAI RLDVTDYKGK
260 270 280 290 300
KFDPKNQKEA QSAVDFLNVS DYVVTDLETK PTSSRPRAPF ITSTLQQTAS
310 320 330 340 350
TRLGFGVKKT MMLAQRLYEA GYITYMRTDS TNLSQDALNM ARTYIENHFG
360 370 380 390 400
AQYLPEKPNF YSSKENAQEA HEAIRPSDIR ALPESLEGME KDAVRLYDLI
410 420 430 440 450
WCQFLACQMP PAQYDSSTLT VTAGDYTLKA KGRILRFDGW TKVLPQIGKN
460 470 480 490 500
PEDQELPSVT VSEKLALKEV QPTQHFTKPP ARFTEAALVK ELEKRGIGRP
510 520 530 540 550
STYAAIISTI QERGYVRTEN RRFYAEKMGE IVTDRLNESF GELMNYDFTA
560 570 580 590 600
NMEDTLDKIA SGSVNWKTEL NQFFKDFSSQ LSKAELDELE GGMRPNSLVE
610 620 630 640 650
TDIKCPTCGR NMAIRTASTG VFLGCTGYAL PPKERCKTTI NLIPEAELLN
660 670 680 690 700
VLDESSETKA LMDRKRCTKC GTAMDSYVID AHRKIHICGN NPNCDGYLIE
710 720 730 740 750
EGSFKIKGYD GPVVECDKCG ADMHLKLGRF GKYMGCTNCD NTRKILKNGE
760 770 780 790 800
VAPPKEEPVH FPELKCEKSD AYFVLRDGAS GVFMSAHNFP KSRETRPVKI
810 820 830 840 850
AELVQYRERL PEKLAYLADA PQKDPEENEA IVRFSRKEKK QYVTSEKEGK
860
ATKWIVDFTN GKWVERKK
Length:868
Mass (Da):98,144
Last modified:November 1, 1995 - v1
Checksum:iAAD604BDEBC75D20
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti343 – 3431T → S in AAC23012. (PubMed:7542800)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20964 Genomic DNA. Translation: AAC43727.1.
L42023 Genomic DNA. Translation: AAC23012.1.
PIRiG64119.
RefSeqiNP_439516.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC23012; AAC23012; HI_1365.
GeneIDi950025.
KEGGihin:HI1365.
PATRICi20191417. VBIHaeInf48452_1419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20964 Genomic DNA. Translation: AAC43727.1 .
L42023 Genomic DNA. Translation: AAC23012.1 .
PIRi G64119.
RefSeqi NP_439516.1. NC_000907.1.

3D structure databases

ProteinModelPortali P43012.
SMRi P43012. Positions 3-598, 748-868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI1365.

Proteomic databases

PRIDEi P43012.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC23012 ; AAC23012 ; HI_1365 .
GeneIDi 950025.
KEGGi hin:HI1365.
PATRICi 20191417. VBIHaeInf48452_1419.

Phylogenomic databases

eggNOGi COG0551.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the Haemophilus influenzae topA locus: DNA topoisomerase I is required for genetic competence."
    Chandler M.S., Smith R.A.
    Gene 169:25-31(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiTOP1_HAEIN
AccessioniPrimary (citable) accession number: P43012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3