P43010 (PNTB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P) transhydrogenase subunit beta
EC=1.6.1.2

Alternative name(s):
Nicotinamide nucleotide transhydrogenase subunit beta
Pyridine nucleotide transhydrogenase subunit beta

Gene names

Name:	pntB
Ordered Locus Names:	HI_1363

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane By similarity.
Catalytic activity	NADPH + NAD+ = NADP+ + NADH.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PNT beta subunit family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	NAD(P) transhydrogenase subunit beta		PRO_0000199026
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Transmembrane	132 – 152	21	Helical; Potential
	Transmembrane	181 – 200	20	Helical; Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Transmembrane	229 – 249	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Transmembrane	321 – 341	21	Helical; Potential
Experimental info
	Sequence conflict	343	1	A → P in AAC43725. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43010 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5F914630211FF844	FASTA	474	50,163
	10 20 30 40 50 60 MSEGLVQAAY ILAALLFIMS LAGLSKHETA KAGCWFGIVG MTIALIATIF GPHSEGTFWI 70 80 90 100 110 120 IIAMIIGGAI GVQRALKVEM TEMPELVAIL HSFVGLAAVL VGFNSYGLHH EALMPEGLDA 130 140 150 160 170 180 AAQAAFVAEQ AVLTNIHNVE VFLGIFIGAV TFTGSVVAFG KLSGKINSKA LMLPHRHKLN 190 200 210 220 230 240 LAALVVSALL MVAFLNNPES IFPVLLMTAI ALAFGWHLVA SIGGADMPVV VSMLNSYSGW 250 260 270 280 290 300 AAAAAGFILN NDLLIVTGAL VGSSGAILSY IMCKAMNRSF VSVIAGGFGN DVQVSSSEEQ 310 320 330 340 350 360 GEHRETTAEE VAELLKNASS VIITPGYGMA VAQAQYPVAD ITAKLRERGV NVRFGIHPVA 370 380 390 400 410 420 GRLPGHMNVL LAEAKVPYDV VLEMDEINDD FADTDVVLVI GANDTVNPAA MEDPNSPIAG 430 440 450 460 470 MPVLEVWKAQ NVIVFKRSMA VGYAGVQNPL FFKENTQMLF GDAKDRVEDI LKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Characterization of the Haemophilus influenzae topA locus: DNA topoisomerase I is required for genetic competence." Chandler M.S., Smith R.A. Gene 169:25-31(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-474. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC23010.1. U20964 Genomic DNA. Translation: AAC43725.1.
PIR	F64119.
RefSeq	NP_439514.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P43010.
SMR	P43010. Positions 308-474.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1363.
Protocols and materials databases
DNASU	950280.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC23010; AAC23010; HI_1363.
GeneID	950280.
KEGG	hin:HI1363.
PATRIC	20191413. VBIHaeInf48452_1417.
Phylogenomic databases
eggNOG	COG1282.
KO	K00325.
OMA	IHEIEIY.
ProtClustDB	PRK09444.
Family and domain databases
InterPro	IPR012136. NADH_DH_b. [Graphical view]
Pfam	PF02233. PNTB. 1 hit. [Graphical view]
PIRSF	PIRSF000204. PNTB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNTB_HAEIN

Accession

Primary (citable) accession number: P43010

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program