P43007 (SATT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutral amino acid transporter A

Alternative name(s):
Alanine/serine/cysteine/threonine transporter 1
Short name=ASCT-1
SATT
Solute carrier family 1 member 4

Gene names

Name:	SLC1A4
Synonyms:	ASCT1, SATT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence.
Subcellular location	Membrane; Multi-pass membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.7
Tissue specificity	Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A4 subfamily. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular nitrogen compound metabolic process Traceable author statement. Source: Reactome cognition Inferred from mutant phenotype. Source: UniProtKB synaptic transmission, glutamatergic Non-traceable author statement. Source: UniProtKB
Cellular component	integral to plasma membrane Inferred by curator. Source: UniProtKB intermediate filament Inferred from sequence or structural similarity. Source: UniProtKB melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-alanine transmembrane transporter activity Inferred from direct assay Ref.1Ref.2. Source: UniProtKB L-cystine transmembrane transporter activity Inferred from direct assay Ref.1Ref.2. Source: UniProtKB L-hydroxyproline transmembrane transporter activity Inferred from direct assay. Source: UniProtKB L-proline transmembrane transporter activity Inferred from direct assay. Source: UniProtKB L-serine transmembrane transporter activity Inferred from direct assay Ref.1Ref.2. Source: UniProtKB L-threonine transmembrane transporter activity Inferred from direct assay Ref.2. Source: UniProtKB chloride channel activity Inferred from direct assay. Source: UniProtKB sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Neutral amino acid transporter A		PRO_0000202079
Regions
	Topological domain	1 – 41	41	Cytoplasmic Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Topological domain	140 – 216	77	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Transmembrane	298 – 318	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
	Transmembrane	418 – 438	21	Helical; Potential
Amino acid modifications
	Modified residue	506	1	Phosphothreonine By similarity
	Modified residue	507	1	Phosphoserine Ref.8
	Glycosylation	201	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	206	1	N-linked (GlcNAc...) Ref.9
Natural variations
	Natural variant	37	1	G → R. Corresponds to variant rs1064512 [ dbSNP | Ensembl ].		VAR_011878
	Natural variant	399	1	V → I. Corresponds to variant rs759458 [ dbSNP | Ensembl ].		VAR_011879
Experimental info
	Sequence conflict	117	1	A → R in AAA02761. Ref.1
	Sequence conflict	127	1	S → T in AAA19438. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P43007 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 57925860042FCEB6	FASTA	532	55,723
	10 20 30 40 50 60 MEKSNETNGY LDSAQAGPAA GPGAPGTAAG RARRCAGFLR RQALVLLTVS GVLAGAGLGA 70 80 90 100 110 120 ALRGLSLSRT QVTYLAFPGE MLLRMLRMII LPLVVCSLVS GAASLDASCL GRLGGIAVAY 130 140 150 160 170 180 FGLTTLSASA LAVALAFIIK PGSGAQTLQS SDLGLEDSGP PPVPKETVDS FLDLARNLFP 190 200 210 220 230 240 SNLVVAAFRT YATDYKVVTQ NSSSGNVTHE KIPIGTEIEG MNILGLVLFA LVLGVALKKL 250 260 270 280 290 300 GSEGEDLIRF FNSLNEATMV LVSWIMWYVP VGIMFLVGSK IVEMKDIIVL VTSLGKYIFA 310 320 330 340 350 360 SILGHVIHGG IVLPLIYFVF TRKNPFRFLL GLLAPFATAF ATCSSSATLP SMMKCIEENN 370 380 390 400 410 420 GVDKRISRFI LPIGATVNMD GAAIFQCVAA VFIAQLNNVE LNAGQIFTIL VTATASSVGA 430 440 450 460 470 480 AGVPAGGVLT IAIILEAIGL PTHDLPLILA VDWIVDRTTT VVNVEGDALG AGILHHLNQK 490 500 510 520 530 ATKKGEQELA EVKVEAIPNC KSEEETSPLV THQNPAGPVA SAPELESKES VL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a human neutral amino acid transporter with structural similarity to the glutamate transporter gene family." Arriza J.L., Kavanaugh M.P., Fairman W.A., Wu Y.-N., Murdoch G.H., North R.A., Amara S.G. J. Biol. Chem. 268:15329-15332(1993) [PubMed: 8101838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain cortex.
[2]	"Cloning and expression of a novel Na(+)-dependent neutral amino acid transporter structurally related to mammalian Na+/glutamate cotransporters." Shafqat S., Tamarappoo B.K., Kilberg M.S., Puranam R.S., McNamara J.O., Guadano-Ferraz A., Fremeau R.T. Jr. J. Biol. Chem. 268:15351-15355(1993) [PubMed: 8340364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hippocampus.
[3]	"Human neutral amino acid transporter ASCT1: structure of the gene (SLC1A4) and localization to chromosome 2p13-p15." Hofmann K., Dueker M., Fink T., Lichter P., Stoffel W. Genomics 24:20-26(1994) [PubMed: 7896285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph and PNS.
[7]	"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes." Chi A., Valencia J.C., Hu Z.-Z., Watabe H., Yamaguchi H., Mangini N.J., Huang H., Canfield V.A., Cheng K.C., Yang F., Abe R., Yamagishi S., Shabanowitz J., Hearing V.J., Wu C., Appella E., Hunt D.F. J. Proteome Res. 5:3135-3144(2006) [PubMed: 17081065] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-507, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-201 AND ASN-206, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L14595 mRNA. Translation: AAA02761.1. L19444 mRNA. Translation: AAA19438.1. U05235 , U05229, U05230, U05231, U05232, U05233, U05234 Genomic DNA. Translation: AAC51349.1. AC007386 Genomic DNA. Translation: AAF03519.1. CH471053 Genomic DNA. Translation: EAW99932.1. CH471053 Genomic DNA. Translation: EAW99933.1. BC026216 mRNA. Translation: AAH26216.1. BC072423 mRNA. Translation: AAH72423.1.
IPI	IPI00015476.
PIR	I37188. I55389.
RefSeq	NP_001180422.1. NM_001193493.1. NP_003029.2. NM_003038.4.
UniGene	Hs.654352.
3D structure databases
ProteinModelPortal	P43007.
SMR	P43007. Positions 40-478.
ModBase	Search...
Protein-protein interaction databases
STRING	P43007.
Protein family/group databases
TCDB	2.A.23.3.1. dicarboxylate/amino acid:cation (Na+ or H+) symporter (DAACS) family.
PTM databases
PhosphoSite	P43007.
Polymorphism databases
DMDM	1173365.
Proteomic databases
PeptideAtlas	P43007.
PRIDE	P43007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000234256; ENSP00000234256; ENSG00000115902.
GeneID	6509.
KEGG	hsa:6509.
UCSC	uc002sdg.1. human.
Organism-specific databases
CTD	6509.
GeneCards	GC02P065128.
H-InvDB	HIX0002106.
HGNC	HGNC:10942. SLC1A4.
HPA	CAB002757.
MIM	600229. gene.
neXtProt	NX_P43007.
PharmGKB	PA35829.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13947.
HOGENOM	HBG739804.
HOVERGEN	HBG000080.
InParanoid	P43007.
OMA	HLNQKAM.
OrthoDB	EOG4BP1BX.
PhylomeDB	P43007.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	P43007.
Bgee	P43007.
CleanEx	HS_SLC1A4.
Genevestigator	P43007.
GermOnline	ENSG00000115902. Homo sapiens.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
KO	K05615.
PANTHER	PTHR11958. Na/diCO_symport. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00160. L-Alanine.
NextBio	25311.
PMAP-CutDB	P43007.
SOURCE	Search...

Entry information

Entry name

SATT_HUMAN

Accession

Primary (citable) accession number: P43007
Secondary accession number(s): D6W5F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.