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P43006

- EAA2_MOUSE

UniProt

P43006 - EAA2_MOUSE

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Protein
Excitatory amino acid transporter 2
Gene
Slc1a2, Eaat2, Glt1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  1. L-glutamate transmembrane transporter activity Source: MGI
  2. glutamate:sodium symporter activity Source: MGI
  3. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. D-aspartate import Source: Ensembl
  2. L-glutamate import Source: Ensembl
  3. L-glutamate transport Source: MGI
  4. adult behavior Source: MGI
  5. cellular response to extracellular stimulus Source: MGI
  6. multicellular organism growth Source: MGI
  7. multicellular organismal aging Source: MGI
  8. nervous system development Source: MGI
  9. positive regulation of glucose import Source: MGI
  10. response to amino acid Source: MGI
  11. response to drug Source: MGI
  12. response to light stimulus Source: MGI
  13. response to wounding Source: MGI
  14. telencephalon development Source: MGI
  15. visual behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2
Gene namesi
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:101931. Slc1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei45 – 6420Helical; Reviewed prediction
Add
BLAST
Transmembranei88 – 10821Helical; Reviewed prediction
Add
BLAST
Transmembranei121 – 14222Helical; Reviewed prediction
Add
BLAST
Topological domaini143 – 23896Extracellular Reviewed prediction
Add
BLAST
Transmembranei239 – 25820Helical; Reviewed prediction
Add
BLAST
Transmembranei279 – 30022Helical; Reviewed prediction
Add
BLAST
Transmembranei316 – 33823Helical; Reviewed prediction
Add
BLAST
Transmembranei405 – 42925Helical; Reviewed prediction
Add
BLAST
Transmembranei436 – 45823Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axolemma Source: MGI
  2. axon Source: MGI
  3. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381C → S: Severely impairs glutamate uptake activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Excitatory amino acid transporter 2
PRO_0000202062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi38 – 381S-palmitoyl cysteine1 Publication
Glycosylationi205 – 2051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Modified residuei537 – 5371Phosphotyrosine1 Publication

Post-translational modificationi

Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP43006.
PaxDbiP43006.
PRIDEiP43006.

PTM databases

PhosphoSiteiP43006.

Expressioni

Tissue specificityi

Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

Gene expression databases

ArrayExpressiP43006.
BgeeiP43006.
GenevestigatoriP43006.

Interactioni

Subunit structurei

Homotrimer By similarity. Interacts with AJUBA By similarity.

Protein-protein interaction databases

BioGridi203290. 7 interactions.
IntActiP43006. 7 interactions.
MINTiMINT-4093909.

Structurei

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006. Positions 46-501.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00740000115213.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
KOiK05613.
OMAiFIAFGIC.
OrthoDBiEOG7RV9G2.
PhylomeDBiP43006.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Glt-1 (identifier: P43006-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT    50
VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS 100
LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK 150
KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP 200
SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA 250
FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII 300
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI 350
FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT 400
ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML 450
LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL 500
DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH NSVVIDECKV 550
TLAANGKSAD CSVEEEPWKR EK 572
Length:572
Mass (Da):62,030
Last modified:November 1, 1995 - v1
Checksum:i13C7C30DED40CA81
GO
Isoform Glt-1A (identifier: P43006-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS

Show »
Length:569
Mass (Da):61,771
Checksum:i930251320261AB8B
GO
Isoform Glt-1B (identifier: P43006-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
     551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Show »
Length:558
Mass (Da):60,617
Checksum:iE82305D0F6E4BD91
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MASTEG → MVS in isoform Glt-1A and isoform Glt-1B.
VSP_006264
Alternative sequencei551 – 57222TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B.
VSP_006265Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261D → E in AAA91643. 1 Publication
Sequence conflicti62 – 621G → R in AAA91643. 1 Publication
Sequence conflicti112 – 1121A → V in AAA91643. 1 Publication
Sequence conflicti454 – 4541T → I in AAB71737. 1 Publication
Sequence conflicti525 – 5251K → L in AAB71737. 1 Publication
Sequence conflicti572 – 5721K → EFD in AAA91643. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U11763 mRNA. Translation: AAA77673.1 .
D43796 mRNA. Translation: BAA07854.1 .
U24699 mRNA. Translation: AAA91643.1 .
U75372 mRNA. Translation: AAB71737.1 .
U75373 mRNA. Translation: AAB71738.1 .
AB007810 mRNA. Translation: BAA23770.1 .
AB007811 mRNA. Translation: BAA23771.1 .
AB007812 mRNA. Translation: BAA23772.1 .
CCDSi CCDS16469.1. [P43006-3 ]
CCDS38188.1. [P43006-1 ]
CCDS38189.1. [P43006-2 ]
PIRi A55676.
JC4262.
RefSeqi NP_001070982.1. NM_001077514.3. [P43006-1 ]
NP_001070983.1. NM_001077515.2. [P43006-2 ]
NP_035523.1. NM_011393.2. [P43006-3 ]
UniGenei Mm.267547.
Mm.371582.

3D structure databases

ProteinModelPortali P43006.
SMRi P43006. Positions 46-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203290. 7 interactions.
IntActi P43006. 7 interactions.
MINTi MINT-4093909.

PTM databases

PhosphoSitei P43006.

Proteomic databases

MaxQBi P43006.
PaxDbi P43006.
PRIDEi P43006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005220 ; ENSMUSP00000005220 ; ENSMUSG00000005089 . [P43006-3 ]
ENSMUST00000080210 ; ENSMUSP00000079100 ; ENSMUSG00000005089 . [P43006-1 ]
ENSMUST00000111212 ; ENSMUSP00000106843 ; ENSMUSG00000005089 . [P43006-2 ]
GeneIDi 20511.
KEGGi mmu:20511.
UCSCi uc008lid.2. mouse. [P43006-1 ]

Organism-specific databases

CTDi 6506.
MGIi MGI:101931. Slc1a2.

Phylogenomic databases

eggNOGi COG1301.
GeneTreei ENSGT00740000115213.
HOGENOMi HOG000208776.
HOVERGENi HBG000080.
KOi K05613.
OMAi FIAFGIC.
OrthoDBi EOG7RV9G2.
PhylomeDBi P43006.
TreeFami TF315206.

Miscellaneous databases

ChiTaRSi SLC1A2. mouse.
NextBioi 298703.
PROi P43006.
SOURCEi Search...

Gene expression databases

ArrayExpressi P43006.
Bgeei P43006.
Genevestigatori P43006.

Family and domain databases

Gene3Di 1.10.3860.10. 2 hits.
InterProi IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view ]
PANTHERi PTHR11958. PTHR11958. 1 hit.
Pfami PF00375. SDF. 1 hit.
[Graphical view ]
PRINTSi PR00173. EDTRNSPORT.
SUPFAMi SSF118215. SSF118215. 2 hits.
PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2."
    Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G.
    Genomics 24:218-224(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
    Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
    Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: JCL:ICR.
    Tissue: Cerebellum.
  3. "Molecular characterization of a high-affinity mouse glutamate transporter."
    Sutherland M.L., Delaney T.A., Noebels J.L.
    Gene 162:271-274(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. Peng J.-B., Guo L.-H.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  5. "Tissue specific variants of glutamate transporter GLT-1."
    Utsunomiya-Tate N., Endou H., Kanai Y.
    FEBS Lett. 416:312-316(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: JCL:ICR.
    Tissue: Brain and Liver.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease."
    Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R.
    Neurobiol. Dis. 40:207-215(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.

Entry informationi

Entry nameiEAA2_MOUSE
AccessioniPrimary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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