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P43006

- EAA2_MOUSE

UniProt

P43006 - EAA2_MOUSE

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Protein

Excitatory amino acid transporter 2

Gene

Slc1a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  1. glutamate:sodium symporter activity Source: MGI
  2. L-glutamate transmembrane transporter activity Source: MGI
  3. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. adult behavior Source: MGI
  2. cellular response to extracellular stimulus Source: MGI
  3. D-aspartate import Source: Ensembl
  4. L-glutamate import Source: Ensembl
  5. L-glutamate transport Source: MGI
  6. multicellular organismal aging Source: MGI
  7. multicellular organism growth Source: MGI
  8. nervous system development Source: MGI
  9. positive regulation of glucose import Source: MGI
  10. response to amino acid Source: MGI
  11. response to drug Source: MGI
  12. response to light stimulus Source: MGI
  13. response to wounding Source: MGI
  14. telencephalon development Source: MGI
  15. visual behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_251788. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_255654. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2
Gene namesi
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:101931. Slc1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei45 – 6420HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Transmembranei121 – 14222HelicalSequence AnalysisAdd
BLAST
Topological domaini143 – 23896ExtracellularSequence AnalysisAdd
BLAST
Transmembranei239 – 25820HelicalSequence AnalysisAdd
BLAST
Transmembranei279 – 30022HelicalSequence AnalysisAdd
BLAST
Transmembranei316 – 33823HelicalSequence AnalysisAdd
BLAST
Transmembranei405 – 42925HelicalSequence AnalysisAdd
BLAST
Transmembranei436 – 45823HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axolemma Source: MGI
  2. axon Source: MGI
  3. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381C → S: Severely impairs glutamate uptake activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Excitatory amino acid transporter 2PRO_0000202062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi38 – 381S-palmitoyl cysteine1 Publication
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Modified residuei537 – 5371Phosphotyrosine1 Publication

Post-translational modificationi

Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP43006.
PaxDbiP43006.
PRIDEiP43006.

PTM databases

PhosphoSiteiP43006.

Expressioni

Tissue specificityi

Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

Gene expression databases

BgeeiP43006.
ExpressionAtlasiP43006. baseline and differential.
GenevestigatoriP43006.

Interactioni

Subunit structurei

Homotrimer. Interacts with AJUBA.By similarity

Protein-protein interaction databases

BioGridi203290. 7 interactions.
IntActiP43006. 7 interactions.
MINTiMINT-4093909.

Structurei

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006. Positions 46-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP43006.
KOiK05613.
OMAiFIAFGIC.
OrthoDBiEOG7RV9G2.
PhylomeDBiP43006.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform Glt-1 (identifier: P43006-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT
60 70 80 90 100
VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS
110 120 130 140 150
LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK
160 170 180 190 200
KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP
210 220 230 240 250
SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA
260 270 280 290 300
FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII
310 320 330 340 350
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI
360 370 380 390 400
FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT
410 420 430 440 450
ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML
460 470 480 490 500
LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL
510 520 530 540 550
DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH NSVVIDECKV
560 570
TLAANGKSAD CSVEEEPWKR EK
Length:572
Mass (Da):62,030
Last modified:November 1, 1995 - v1
Checksum:i13C7C30DED40CA81
GO
Isoform Glt-1A (identifier: P43006-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS

Show »
Length:569
Mass (Da):61,771
Checksum:i930251320261AB8B
GO
Isoform Glt-1B (identifier: P43006-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
     551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Show »
Length:558
Mass (Da):60,617
Checksum:iE82305D0F6E4BD91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261D → E in AAA91643. (PubMed:7557442)Curated
Sequence conflicti62 – 621G → R in AAA91643. (PubMed:7557442)Curated
Sequence conflicti112 – 1121A → V in AAA91643. (PubMed:7557442)Curated
Sequence conflicti454 – 4541T → I in AAB71737. 1 PublicationCurated
Sequence conflicti525 – 5251K → L in AAB71737. 1 PublicationCurated
Sequence conflicti572 – 5721K → EFD in AAA91643. (PubMed:7557442)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MASTEG → MVS in isoform Glt-1A and isoform Glt-1B. CuratedVSP_006264
Alternative sequencei551 – 57222TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B. CuratedVSP_006265Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1 .
D43796 mRNA. Translation: BAA07854.1 .
U24699 mRNA. Translation: AAA91643.1 .
U75372 mRNA. Translation: AAB71737.1 .
U75373 mRNA. Translation: AAB71738.1 .
AB007810 mRNA. Translation: BAA23770.1 .
AB007811 mRNA. Translation: BAA23771.1 .
AB007812 mRNA. Translation: BAA23772.1 .
CCDSi CCDS16469.1. [P43006-3 ]
CCDS38188.1. [P43006-1 ]
CCDS38189.1. [P43006-2 ]
PIRi A55676.
JC4262.
RefSeqi NP_001070982.1. NM_001077514.3. [P43006-1 ]
NP_001070983.1. NM_001077515.2. [P43006-2 ]
NP_035523.1. NM_011393.2. [P43006-3 ]
UniGenei Mm.267547.
Mm.371582.

3D structure databases

ProteinModelPortali P43006.
SMRi P43006. Positions 46-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203290. 7 interactions.
IntActi P43006. 7 interactions.
MINTi MINT-4093909.

PTM databases

PhosphoSitei P43006.

Proteomic databases

MaxQBi P43006.
PaxDbi P43006.
PRIDEi P43006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005220 ; ENSMUSP00000005220 ; ENSMUSG00000005089 . [P43006-3 ]
ENSMUST00000080210 ; ENSMUSP00000079100 ; ENSMUSG00000005089 . [P43006-1 ]
ENSMUST00000111212 ; ENSMUSP00000106843 ; ENSMUSG00000005089 . [P43006-2 ]
GeneIDi 20511.
KEGGi mmu:20511.
UCSCi uc008lid.2. mouse. [P43006-1 ]

Organism-specific databases

CTDi 6506.
MGIi MGI:101931. Slc1a2.

Phylogenomic databases

eggNOGi COG1301.
GeneTreei ENSGT00760000119117.
HOGENOMi HOG000208776.
HOVERGENi HBG000080.
InParanoidi P43006.
KOi K05613.
OMAi FIAFGIC.
OrthoDBi EOG7RV9G2.
PhylomeDBi P43006.
TreeFami TF315206.

Enzyme and pathway databases

Reactomei REACT_251788. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_255654. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Miscellaneous databases

ChiTaRSi Slc1a2. mouse.
NextBioi 298703.
PROi P43006.
SOURCEi Search...

Gene expression databases

Bgeei P43006.
ExpressionAtlasi P43006. baseline and differential.
Genevestigatori P43006.

Family and domain databases

Gene3Di 1.10.3860.10. 2 hits.
InterProi IPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view ]
PANTHERi PTHR11958. PTHR11958. 1 hit.
Pfami PF00375. SDF. 1 hit.
[Graphical view ]
PRINTSi PR00173. EDTRNSPORT.
SUPFAMi SSF118215. SSF118215. 2 hits.
PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2."
    Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G.
    Genomics 24:218-224(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
    Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
    Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: JCL:ICR.
    Tissue: Cerebellum.
  3. "Molecular characterization of a high-affinity mouse glutamate transporter."
    Sutherland M.L., Delaney T.A., Noebels J.L.
    Gene 162:271-274(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. Peng J.-B., Guo L.-H.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  5. "Tissue specific variants of glutamate transporter GLT-1."
    Utsunomiya-Tate N., Endou H., Kanai Y.
    FEBS Lett. 416:312-316(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: JCL:ICR.
    Tissue: Brain and Liver.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease."
    Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R.
    Neurobiol. Dis. 40:207-215(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.

Entry informationi

Entry nameiEAA2_MOUSE
AccessioniPrimary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3