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Protein

Excitatory amino acid transporter 2

Gene

Slc1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  1. glutamate:sodium symporter activity Source: MGI
  2. L-glutamate transmembrane transporter activity Source: MGI
  3. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. adult behavior Source: MGI
  2. cellular response to extracellular stimulus Source: MGI
  3. D-aspartate import Source: MGI
  4. L-glutamate import Source: MGI
  5. L-glutamate transmembrane transport Source: MGI
  6. L-glutamate transport Source: MGI
  7. multicellular organismal aging Source: MGI
  8. multicellular organism growth Source: MGI
  9. nervous system development Source: MGI
  10. positive regulation of glucose import Source: MGI
  11. response to amino acid Source: MGI
  12. response to drug Source: MGI
  13. response to light stimulus Source: MGI
  14. response to wounding Source: MGI
  15. telencephalon development Source: MGI
  16. visual behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_298432. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2
Gene namesi
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:101931. Slc1a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei45 – 6420HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Transmembranei121 – 14222HelicalSequence AnalysisAdd
BLAST
Topological domaini143 – 23896ExtracellularSequence AnalysisAdd
BLAST
Transmembranei239 – 25820HelicalSequence AnalysisAdd
BLAST
Transmembranei279 – 30022HelicalSequence AnalysisAdd
BLAST
Transmembranei316 – 33823HelicalSequence AnalysisAdd
BLAST
Transmembranei405 – 42925HelicalSequence AnalysisAdd
BLAST
Transmembranei436 – 45823HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axolemma Source: MGI
  2. axon Source: MGI
  3. integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi38 – 381C → S: Severely impairs glutamate uptake activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Excitatory amino acid transporter 2PRO_0000202062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi38 – 381S-palmitoyl cysteine1 Publication
Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Modified residuei537 – 5371Phosphotyrosine1 Publication

Post-translational modificationi

Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP43006.
PaxDbiP43006.
PRIDEiP43006.

PTM databases

PhosphoSiteiP43006.

Expressioni

Tissue specificityi

Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

Gene expression databases

BgeeiP43006.
ExpressionAtlasiP43006. baseline and differential.
GenevestigatoriP43006.

Interactioni

Subunit structurei

Homotrimer. Interacts with AJUBA.By similarity

Protein-protein interaction databases

BioGridi203290. 7 interactions.
IntActiP43006. 7 interactions.
MINTiMINT-4093909.

Structurei

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006. Positions 46-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP43006.
KOiK05613.
OMAiFIAFGIC.
OrthoDBiEOG7RV9G2.
PhylomeDBiP43006.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Glt-1 (identifier: P43006-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT
60 70 80 90 100
VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS
110 120 130 140 150
LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK
160 170 180 190 200
KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP
210 220 230 240 250
SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA
260 270 280 290 300
FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII
310 320 330 340 350
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI
360 370 380 390 400
FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT
410 420 430 440 450
ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML
460 470 480 490 500
LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL
510 520 530 540 550
DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH NSVVIDECKV
560 570
TLAANGKSAD CSVEEEPWKR EK
Length:572
Mass (Da):62,030
Last modified:November 1, 1995 - v1
Checksum:i13C7C30DED40CA81
GO
Isoform Glt-1A (identifier: P43006-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS

Show »
Length:569
Mass (Da):61,771
Checksum:i930251320261AB8B
GO
Isoform Glt-1B (identifier: P43006-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
     551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Show »
Length:558
Mass (Da):60,617
Checksum:iE82305D0F6E4BD91
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261D → E in AAA91643 (PubMed:7557442).Curated
Sequence conflicti62 – 621G → R in AAA91643 (PubMed:7557442).Curated
Sequence conflicti112 – 1121A → V in AAA91643 (PubMed:7557442).Curated
Sequence conflicti454 – 4541T → I in AAB71737 (Ref. 4) Curated
Sequence conflicti525 – 5251K → L in AAB71737 (Ref. 4) Curated
Sequence conflicti572 – 5721K → EFD in AAA91643 (PubMed:7557442).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MASTEG → MVS in isoform Glt-1A and isoform Glt-1B. CuratedVSP_006264
Alternative sequencei551 – 57222TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B. CuratedVSP_006265Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006. Positions 46-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203290. 7 interactions.
IntActiP43006. 7 interactions.
MINTiMINT-4093909.

PTM databases

PhosphoSiteiP43006.

Proteomic databases

MaxQBiP43006.
PaxDbiP43006.
PRIDEiP43006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Organism-specific databases

CTDi6506.
MGIiMGI:101931. Slc1a2.

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP43006.
KOiK05613.
OMAiFIAFGIC.
OrthoDBiEOG7RV9G2.
PhylomeDBiP43006.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_277017. Transport of inorganic cations/anions and amino acids/oligopeptides.
REACT_298432. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Miscellaneous databases

ChiTaRSiSlc1a2. mouse.
NextBioi298703.
PROiP43006.
SOURCEiSearch...

Gene expression databases

BgeeiP43006.
ExpressionAtlasiP43006. baseline and differential.
GenevestigatoriP43006.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2."
    Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G.
    Genomics 24:218-224(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
    Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
    Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: JCL:ICR.
    Tissue: Cerebellum.
  3. "Molecular characterization of a high-affinity mouse glutamate transporter."
    Sutherland M.L., Delaney T.A., Noebels J.L.
    Gene 162:271-274(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. Peng J.-B., Guo L.-H.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  5. "Tissue specific variants of glutamate transporter GLT-1."
    Utsunomiya-Tate N., Endou H., Kanai Y.
    FEBS Lett. 416:312-316(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Strain: JCL:ICR.
    Tissue: Brain and Liver.
  6. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease."
    Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R.
    Neurobiol. Dis. 40:207-215(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.

Entry informationi

Entry nameiEAA2_MOUSE
AccessioniPrimary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.