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Protein

Excitatory amino acid transporter 2

Gene

Slc1a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

GO - Molecular functioni

  • anion transmembrane transporter activity Source: MGI
  • glutamate:sodium symporter activity Source: MGI
  • L-glutamate transmembrane transporter activity Source: MGI

GO - Biological processi

  • adult behavior Source: MGI
  • anion transmembrane transport Source: MGI
  • cellular response to extracellular stimulus Source: MGI
  • D-aspartate import Source: MGI
  • L-glutamate import Source: MGI
  • L-glutamate transmembrane transport Source: MGI
  • L-glutamate transport Source: MGI
  • multicellular organism aging Source: MGI
  • multicellular organism growth Source: MGI
  • nervous system development Source: MGI
  • positive regulation of glucose import Source: MGI
  • response to amino acid Source: MGI
  • response to drug Source: MGI
  • response to light stimulus Source: MGI
  • response to wounding Source: MGI
  • telencephalon development Source: MGI
  • visual behavior Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2
Gene namesi
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:101931. Slc1a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44CytoplasmicSequence analysisAdd BLAST44
Transmembranei45 – 64HelicalSequence analysisAdd BLAST20
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei121 – 142HelicalSequence analysisAdd BLAST22
Topological domaini143 – 238ExtracellularSequence analysisAdd BLAST96
Transmembranei239 – 258HelicalSequence analysisAdd BLAST20
Transmembranei279 – 300HelicalSequence analysisAdd BLAST22
Transmembranei316 – 338HelicalSequence analysisAdd BLAST23
Transmembranei405 – 429HelicalSequence analysisAdd BLAST25
Transmembranei436 – 458HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

  • axolemma Source: MGI
  • axon Source: MGI
  • cell surface Source: MGI
  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38C → S: Severely impairs glutamate uptake activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020621 – 572Excitatory amino acid transporter 2Add BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Modified residuei21PhosphoserineBy similarity1
Modified residuei24PhosphoserineCombined sources1
Modified residuei25PhosphoserineBy similarity1
Lipidationi38S-palmitoyl cysteine1 Publication1
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Modified residuei505PhosphoserineCombined sources1
Modified residuei520PhosphoserineCombined sources1
Modified residuei530PhosphoserineCombined sources1
Modified residuei532PhosphoserineCombined sources1
Modified residuei537PhosphotyrosineCombined sources1
Modified residuei542PhosphoserineCombined sources1
Modified residuei558PhosphoserineCombined sources1
Modified residuei562PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.
Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP43006.
PeptideAtlasiP43006.
PRIDEiP43006.

PTM databases

iPTMnetiP43006.
PhosphoSitePlusiP43006.
SwissPalmiP43006.

Expressioni

Tissue specificityi

Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

Gene expression databases

BgeeiENSMUSG00000005089.
ExpressionAtlasiP43006. baseline and differential.
GenevisibleiP43006. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with AJUBA.By similarity

Protein-protein interaction databases

BioGridi203290. 6 interactors.
IntActiP43006. 8 interactors.
MINTiMINT-4093909.
STRINGi10090.ENSMUSP00000079100.

Structurei

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP43006.
KOiK05613.
OMAiIEMMTKT.
OrthoDBiEOG091G0UCE.
PhylomeDBiP43006.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Glt-1 (identifier: P43006-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT
60 70 80 90 100
VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS
110 120 130 140 150
LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK
160 170 180 190 200
KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP
210 220 230 240 250
SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA
260 270 280 290 300
FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII
310 320 330 340 350
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI
360 370 380 390 400
FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT
410 420 430 440 450
ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML
460 470 480 490 500
LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL
510 520 530 540 550
DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH NSVVIDECKV
560 570
TLAANGKSAD CSVEEEPWKR EK
Length:572
Mass (Da):62,030
Last modified:November 1, 1995 - v1
Checksum:i13C7C30DED40CA81
GO
Isoform Glt-1A (identifier: P43006-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS

Show »
Length:569
Mass (Da):61,771
Checksum:i930251320261AB8B
GO
Isoform Glt-1B (identifier: P43006-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
     551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Show »
Length:558
Mass (Da):60,617
Checksum:iE82305D0F6E4BD91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26D → E in AAA91643 (PubMed:7557442).Curated1
Sequence conflicti62G → R in AAA91643 (PubMed:7557442).Curated1
Sequence conflicti112A → V in AAA91643 (PubMed:7557442).Curated1
Sequence conflicti454T → I in AAB71737 (Ref. 4) Curated1
Sequence conflicti525K → L in AAB71737 (Ref. 4) Curated1
Sequence conflicti572K → EFD in AAA91643 (PubMed:7557442).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0062641 – 6MASTEG → MVS in isoform Glt-1A and isoform Glt-1B. Curated6
Alternative sequenceiVSP_006265551 – 572TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.
Mm.453107.
Mm.453110.

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
CCDSiCCDS16469.1. [P43006-3]
CCDS38188.1. [P43006-1]
CCDS38189.1. [P43006-2]
PIRiA55676.
JC4262.
RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
NP_001070983.1. NM_001077515.2. [P43006-2]
NP_035523.1. NM_011393.2. [P43006-3]
UniGeneiMm.267547.
Mm.371582.
Mm.453107.
Mm.453110.

3D structure databases

ProteinModelPortaliP43006.
SMRiP43006.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203290. 6 interactors.
IntActiP43006. 8 interactors.
MINTiMINT-4093909.
STRINGi10090.ENSMUSP00000079100.

PTM databases

iPTMnetiP43006.
PhosphoSitePlusiP43006.
SwissPalmiP43006.

Proteomic databases

PaxDbiP43006.
PeptideAtlasiP43006.
PRIDEiP43006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneIDi20511.
KEGGimmu:20511.
UCSCiuc008lid.2. mouse. [P43006-1]

Organism-specific databases

CTDi6506.
MGIiMGI:101931. Slc1a2.

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiP43006.
KOiK05613.
OMAiIEMMTKT.
OrthoDBiEOG091G0UCE.
PhylomeDBiP43006.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiR-MMU-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

ChiTaRSiSlc1a2. mouse.
PROiP43006.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005089.
ExpressionAtlasiP43006. baseline and differential.
GenevisibleiP43006. MM.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 2 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAA2_MOUSE
AccessioniPrimary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.