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P43006

- EAA2_MOUSE

UniProt

P43006 - EAA2_MOUSE

Protein

Excitatory amino acid transporter 2

Gene

Slc1a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

    GO - Molecular functioni

    1. glutamate:sodium symporter activity Source: MGI
    2. L-glutamate transmembrane transporter activity Source: MGI
    3. sodium:dicarboxylate symporter activity Source: InterPro

    GO - Biological processi

    1. adult behavior Source: MGI
    2. cellular response to extracellular stimulus Source: MGI
    3. D-aspartate import Source: Ensembl
    4. L-glutamate import Source: Ensembl
    5. L-glutamate transport Source: MGI
    6. multicellular organismal aging Source: MGI
    7. multicellular organism growth Source: MGI
    8. nervous system development Source: MGI
    9. positive regulation of glucose import Source: MGI
    10. response to amino acid Source: MGI
    11. response to drug Source: MGI
    12. response to light stimulus Source: MGI
    13. response to wounding Source: MGI
    14. telencephalon development Source: MGI
    15. visual behavior Source: MGI

    Keywords - Biological processi

    Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Excitatory amino acid transporter 2
    Alternative name(s):
    GLT-1
    Sodium-dependent glutamate/aspartate transporter 2
    Solute carrier family 1 member 2
    Gene namesi
    Name:Slc1a2
    Synonyms:Eaat2, Glt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:101931. Slc1a2.

    Subcellular locationi

    GO - Cellular componenti

    1. axolemma Source: MGI
    2. axon Source: MGI
    3. integral component of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi38 – 381C → S: Severely impairs glutamate uptake activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Excitatory amino acid transporter 2PRO_0000202062Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi38 – 381S-palmitoyl cysteine1 Publication
    Glycosylationi205 – 2051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Modified residuei537 – 5371Phosphotyrosine1 Publication

    Post-translational modificationi

    Glycosylated.
    Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.1 Publication

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP43006.
    PaxDbiP43006.
    PRIDEiP43006.

    PTM databases

    PhosphoSiteiP43006.

    Expressioni

    Tissue specificityi

    Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

    Gene expression databases

    ArrayExpressiP43006.
    BgeeiP43006.
    GenevestigatoriP43006.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with AJUBA.By similarity

    Protein-protein interaction databases

    BioGridi203290. 7 interactions.
    IntActiP43006. 7 interactions.
    MINTiMINT-4093909.

    Structurei

    3D structure databases

    ProteinModelPortaliP43006.
    SMRiP43006. Positions 46-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4444CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini143 – 23896ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei45 – 6420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei239 – 25820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 30022HelicalSequence AnalysisAdd
    BLAST
    Transmembranei316 – 33823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei405 – 42925HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45823HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1301.
    GeneTreeiENSGT00740000115213.
    HOGENOMiHOG000208776.
    HOVERGENiHBG000080.
    KOiK05613.
    OMAiFIAFGIC.
    OrthoDBiEOG7RV9G2.
    PhylomeDBiP43006.
    TreeFamiTF315206.

    Family and domain databases

    Gene3Di1.10.3860.10. 2 hits.
    InterProiIPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 2 hits.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Glt-1 (identifier: P43006-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT    50
    VFGVILGAVC GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS 100
    LITGLSGLDA KASGRLGTRA MVYYMSTTII AAVLGVILVL AIHPGNPKLK 150
    KQLGPGKKND EVSSLDAFLD LIRNLFPENL VQACFQQIQT VTKKVLVAPP 200
    SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN VLGLIGFFIA 250
    FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII 300
    AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI 350
    FQAWITALGT ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT 400
    ALYEAVAAIF IAQMNGVILD GGQIVTVSLT ATLASIGAAS IPSAGLVTML 450
    LILTAVGLPT EDISLLVAVD WLLDRMRTSV NVVGDSFGAG IVYHLSKSEL 500
    DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH NSVVIDECKV 550
    TLAANGKSAD CSVEEEPWKR EK 572
    Length:572
    Mass (Da):62,030
    Last modified:November 1, 1995 - v1
    Checksum:i13C7C30DED40CA81
    GO
    Isoform Glt-1A (identifier: P43006-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: MASTEG → MVS

    Show »
    Length:569
    Mass (Da):61,771
    Checksum:i930251320261AB8B
    GO
    Isoform Glt-1B (identifier: P43006-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: MASTEG → MVS
         551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

    Show »
    Length:558
    Mass (Da):60,617
    Checksum:iE82305D0F6E4BD91
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261D → E in AAA91643. (PubMed:7557442)Curated
    Sequence conflicti62 – 621G → R in AAA91643. (PubMed:7557442)Curated
    Sequence conflicti112 – 1121A → V in AAA91643. (PubMed:7557442)Curated
    Sequence conflicti454 – 4541T → I in AAB71737. 1 PublicationCurated
    Sequence conflicti525 – 5251K → L in AAB71737. 1 PublicationCurated
    Sequence conflicti572 – 5721K → EFD in AAA91643. (PubMed:7557442)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 66MASTEG → MVS in isoform Glt-1A and isoform Glt-1B. CuratedVSP_006264
    Alternative sequencei551 – 57222TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B. CuratedVSP_006265Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11763 mRNA. Translation: AAA77673.1.
    D43796 mRNA. Translation: BAA07854.1.
    U24699 mRNA. Translation: AAA91643.1.
    U75372 mRNA. Translation: AAB71737.1.
    U75373 mRNA. Translation: AAB71738.1.
    AB007810 mRNA. Translation: BAA23770.1.
    AB007811 mRNA. Translation: BAA23771.1.
    AB007812 mRNA. Translation: BAA23772.1.
    CCDSiCCDS16469.1. [P43006-3]
    CCDS38188.1. [P43006-1]
    CCDS38189.1. [P43006-2]
    PIRiA55676.
    JC4262.
    RefSeqiNP_001070982.1. NM_001077514.3. [P43006-1]
    NP_001070983.1. NM_001077515.2. [P43006-2]
    NP_035523.1. NM_011393.2. [P43006-3]
    UniGeneiMm.267547.
    Mm.371582.

    Genome annotation databases

    EnsembliENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
    ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
    ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
    GeneIDi20511.
    KEGGimmu:20511.
    UCSCiuc008lid.2. mouse. [P43006-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11763 mRNA. Translation: AAA77673.1 .
    D43796 mRNA. Translation: BAA07854.1 .
    U24699 mRNA. Translation: AAA91643.1 .
    U75372 mRNA. Translation: AAB71737.1 .
    U75373 mRNA. Translation: AAB71738.1 .
    AB007810 mRNA. Translation: BAA23770.1 .
    AB007811 mRNA. Translation: BAA23771.1 .
    AB007812 mRNA. Translation: BAA23772.1 .
    CCDSi CCDS16469.1. [P43006-3 ]
    CCDS38188.1. [P43006-1 ]
    CCDS38189.1. [P43006-2 ]
    PIRi A55676.
    JC4262.
    RefSeqi NP_001070982.1. NM_001077514.3. [P43006-1 ]
    NP_001070983.1. NM_001077515.2. [P43006-2 ]
    NP_035523.1. NM_011393.2. [P43006-3 ]
    UniGenei Mm.267547.
    Mm.371582.

    3D structure databases

    ProteinModelPortali P43006.
    SMRi P43006. Positions 46-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203290. 7 interactions.
    IntActi P43006. 7 interactions.
    MINTi MINT-4093909.

    PTM databases

    PhosphoSitei P43006.

    Proteomic databases

    MaxQBi P43006.
    PaxDbi P43006.
    PRIDEi P43006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005220 ; ENSMUSP00000005220 ; ENSMUSG00000005089 . [P43006-3 ]
    ENSMUST00000080210 ; ENSMUSP00000079100 ; ENSMUSG00000005089 . [P43006-1 ]
    ENSMUST00000111212 ; ENSMUSP00000106843 ; ENSMUSG00000005089 . [P43006-2 ]
    GeneIDi 20511.
    KEGGi mmu:20511.
    UCSCi uc008lid.2. mouse. [P43006-1 ]

    Organism-specific databases

    CTDi 6506.
    MGIi MGI:101931. Slc1a2.

    Phylogenomic databases

    eggNOGi COG1301.
    GeneTreei ENSGT00740000115213.
    HOGENOMi HOG000208776.
    HOVERGENi HBG000080.
    KOi K05613.
    OMAi FIAFGIC.
    OrthoDBi EOG7RV9G2.
    PhylomeDBi P43006.
    TreeFami TF315206.

    Miscellaneous databases

    ChiTaRSi SLC1A2. mouse.
    NextBioi 298703.
    PROi P43006.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P43006.
    Bgeei P43006.
    Genevestigatori P43006.

    Family and domain databases

    Gene3Di 1.10.3860.10. 2 hits.
    InterProi IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view ]
    PANTHERi PTHR11958. PTHR11958. 1 hit.
    Pfami PF00375. SDF. 1 hit.
    [Graphical view ]
    PRINTSi PR00173. EDTRNSPORT.
    SUPFAMi SSF118215. SSF118215. 2 hits.
    PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2."
      Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G.
      Genomics 24:218-224(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Molecular cloning of two glutamate transporter subtypes from mouse brain."
      Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
      Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: JCL:ICR.
      Tissue: Cerebellum.
    3. "Molecular characterization of a high-affinity mouse glutamate transporter."
      Sutherland M.L., Delaney T.A., Noebels J.L.
      Gene 162:271-274(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. Peng J.-B., Guo L.-H.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    5. "Tissue specific variants of glutamate transporter GLT-1."
      Utsunomiya-Tate N., Endou H., Kanai Y.
      FEBS Lett. 416:312-316(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Strain: JCL:ICR.
      Tissue: Brain and Liver.
    6. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease."
      Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R.
      Neurobiol. Dis. 40:207-215(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.

    Entry informationi

    Entry nameiEAA2_MOUSE
    AccessioniPrimary (citable) accession number: P43006
    Secondary accession number(s): O35877, O54686, O54687
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3