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P43006 (EAA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 2
Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2
Gene names
Name:Slc1a2
Synonyms:Eaat2, Glt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length572 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Subunit structure

Homotrimer By similarity. Interacts with AJUBA By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Isoform GLT1is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.

Post-translational modification

Glycosylated.

Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity. Ref.8

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily. [View classification]

Ontologies

Keywords
   Biological processSymport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processD-aspartate import

Inferred from electronic annotation. Source: Ensembl

L-glutamate import

Inferred from electronic annotation. Source: Ensembl

L-glutamate transport

Inferred from direct assay Ref.1. Source: MGI

adult behavior

Inferred from mutant phenotype PubMed 9180080. Source: MGI

cellular response to extracellular stimulus

Inferred from mutant phenotype PubMed 16197522. Source: MGI

multicellular organism growth

Inferred from mutant phenotype PubMed 9180080. Source: MGI

multicellular organismal aging

Inferred from mutant phenotype PubMed 9180080. Source: MGI

nervous system development

Inferred from mutant phenotype PubMed 9180080. Source: MGI

positive regulation of glucose import

Inferred from mutant phenotype PubMed 16197522. Source: MGI

response to amino acid

Inferred from mutant phenotype PubMed 9180080. Source: MGI

response to drug

Inferred from mutant phenotype PubMed 9180080PubMed 9539795. Source: MGI

response to light stimulus

Inferred from mutant phenotype PubMed 9539795. Source: MGI

response to wounding

Inferred from mutant phenotype PubMed 9180080. Source: MGI

telencephalon development

Inferred from mutant phenotype PubMed 9180080. Source: MGI

visual behavior

Inferred from mutant phenotype PubMed 16197522. Source: MGI

   Cellular_componentaxolemma

Inferred from direct assay PubMed 9671661. Source: MGI

axon

Inferred from direct assay PubMed 9671661. Source: MGI

integral component of plasma membrane

Inferred by curator Ref.1. Source: MGI

   Molecular_functionL-glutamate transmembrane transporter activity

Inferred from mutant phenotype PubMed 15009636. Source: MGI

glutamate:sodium symporter activity

Inferred from direct assay Ref.1. Source: MGI

sodium:dicarboxylate symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Glt-1 (identifier: P43006-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Glt-1A (identifier: P43006-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
Isoform Glt-1B (identifier: P43006-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MASTEG → MVS
     551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 572572Excitatory amino acid transporter 2
PRO_0000202062

Regions

Topological domain1 – 4444Cytoplasmic Potential
Transmembrane45 – 6420Helical; Potential
Transmembrane88 – 10821Helical; Potential
Transmembrane121 – 14222Helical; Potential
Topological domain143 – 23896Extracellular Potential
Transmembrane239 – 25820Helical; Potential
Transmembrane279 – 30022Helical; Potential
Transmembrane316 – 33823Helical; Potential
Transmembrane405 – 42925Helical; Potential
Transmembrane436 – 45823Helical; Potential

Amino acid modifications

Modified residue5371Phosphotyrosine Ref.7
Lipidation381S-palmitoyl cysteine Ref.8
Glycosylation2051N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 66MASTEG → MVS in isoform Glt-1A and isoform Glt-1B.
VSP_006264
Alternative sequence551 – 57222TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B.
VSP_006265

Experimental info

Mutagenesis381C → S: Severely impairs glutamate uptake activity. Ref.8
Sequence conflict261D → E in AAA91643. Ref.3
Sequence conflict621G → R in AAA91643. Ref.3
Sequence conflict1121A → V in AAA91643. Ref.3
Sequence conflict4541T → I in AAB71737. Ref.4
Sequence conflict5251K → L in AAB71737. Ref.4
Sequence conflict5721K → EFD in AAA91643. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Glt-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 13C7C30DED40CA81

FASTA57262,030
        10         20         30         40         50         60 
MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT VFGVILGAVC 

        70         80         90        100        110        120 
GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA 

       130        140        150        160        170        180 
MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL 

       190        200        210        220        230        240 
VQACFQQIQT VTKKVLVAPP SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN 

       250        260        270        280        290        300 
VLGLIGFFIA FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII 

       310        320        330        340        350        360 
AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI FQAWITALGT 

       370        380        390        400        410        420 
ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT ALYEAVAAIF IAQMNGVILD 

       430        440        450        460        470        480 
GGQIVTVSLT ATLASIGAAS IPSAGLVTML LILTAVGLPT EDISLLVAVD WLLDRMRTSV 

       490        500        510        520        530        540 
NVVGDSFGAG IVYHLSKSEL DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH 

       550        560        570 
NSVVIDECKV TLAANGKSAD CSVEEEPWKR EK 

« Hide

Isoform Glt-1A [UniParc].

Checksum: 930251320261AB8B
Show »

FASTA56961,771
Isoform Glt-1B [UniParc].

Checksum: E82305D0F6E4BD91
Show »

FASTA55860,617

References

« Hide 'large scale' references
[1]"Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2."
Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G.
Genomics 24:218-224(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"Molecular cloning of two glutamate transporter subtypes from mouse brain."
Mukainaka Y., Tanaka K., Hagiwara T., Wada K.
Biochim. Biophys. Acta 1244:233-237(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: JCL:ICR.
Tissue: Cerebellum.
[3]"Molecular characterization of a high-affinity mouse glutamate transporter."
Sutherland M.L., Delaney T.A., Noebels J.L.
Gene 162:271-274(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]Peng J.-B., Guo L.-H.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[5]"Tissue specific variants of glutamate transporter GLT-1."
Utsunomiya-Tate N., Endou H., Kanai Y.
FEBS Lett. 416:312-316(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: JCL:ICR.
Tissue: Brain and Liver.
[6]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[7]"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[8]"Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease."
Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R.
Neurobiol. Dis. 40:207-215(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11763 mRNA. Translation: AAA77673.1.
D43796 mRNA. Translation: BAA07854.1.
U24699 mRNA. Translation: AAA91643.1.
U75372 mRNA. Translation: AAB71737.1.
U75373 mRNA. Translation: AAB71738.1.
AB007810 mRNA. Translation: BAA23770.1.
AB007811 mRNA. Translation: BAA23771.1.
AB007812 mRNA. Translation: BAA23772.1.
PIRA55676.
JC4262.
RefSeqNP_001070982.1. NM_001077514.3.
NP_001070983.1. NM_001077515.2.
NP_035523.1. NM_011393.2.
UniGeneMm.267547.
Mm.371582.

3D structure databases

ProteinModelPortalP43006.
SMRP43006. Positions 46-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203290. 7 interactions.
IntActP43006. 7 interactions.
MINTMINT-4093909.

PTM databases

PhosphoSiteP43006.

Proteomic databases

PaxDbP43006.
PRIDEP43006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. [P43006-3]
ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. [P43006-1]
ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089. [P43006-2]
GeneID20511.
KEGGmmu:20511.
UCSCuc008lid.2. mouse. [P43006-1]

Organism-specific databases

CTD6506.
MGIMGI:101931. Slc1a2.

Phylogenomic databases

eggNOGCOG1301.
GeneTreeENSGT00740000115213.
HOGENOMHOG000208776.
HOVERGENHBG000080.
KOK05613.
OMAVMVIAFP.
OrthoDBEOG7RV9G2.
PhylomeDBP43006.
TreeFamTF315206.

Gene expression databases

ArrayExpressP43006.
BgeeP43006.
GenevestigatorP43006.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC1A2. mouse.
NextBio298703.
PROP43006.
SOURCESearch...

Entry information

Entry nameEAA2_MOUSE
AccessionPrimary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot