Excitatory amino acid transporter 2

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P43006 (EAA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 106. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Excitatory amino acid transporter 2

Alternative name(s):
GLT-1
Sodium-dependent glutamate/aspartate transporter 2
Solute carrier family 1 member 2

Gene names

Name:	Slc1a2
Synonyms:	Eaat2, Glt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Subunit structure	Homotrimer By similarity. Interacts with AJUBA By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Isoform GLT1 is expressed in the brain. Isoforms GLT-1A and GLT-1B are expressed in the liver.
Post-translational modification	Glycosylated. Palmitoylation at Cys-38 is not required for correct subcellular localization, but is important for glutamate uptake activity.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	adult behavior Inferred from mutant phenotype. Source: MGI cellular response to extracellular stimulus Inferred from mutant phenotype. Source: MGI multicellular organism growth Inferred from mutant phenotype. Source: MGI multicellular organismal aging Inferred from mutant phenotype. Source: MGI positive regulation of glucose import Inferred from mutant phenotype. Source: MGI response to amino acid stimulus Inferred from mutant phenotype. Source: MGI response to drug Inferred from mutant phenotype. Source: MGI response to wounding Inferred from mutant phenotype. Source: MGI telencephalon development Inferred from mutant phenotype. Source: MGI visual behavior Inferred from mutant phenotype. Source: MGI
Cellular component	axolemma Inferred from direct assay. Source: MGI integral to plasma membrane Inferred by curator Ref.1. Source: MGI
Molecular function	glutamate:sodium symporter activity Inferred from direct assay Ref.1. Source: MGI sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Glt-1 (identifier: P43006-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Glt-1A (identifier: P43006-2) The sequence of this isoform differs from the canonical sequence as follows: 1-6: MASTEG → MVS

Isoform Glt-1B (identifier: P43006-3) The sequence of this isoform differs from the canonical sequence as follows: 1-6: MASTEG → MVS 551-572: TLAANGKSADCSVEEEPWKREK → PFPFLDIETCI

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 572

572

Excitatory amino acid transporter 2

PRO_0000202062

Regions

Topological domain

1 – 44

Cytoplasmic Potential

Transmembrane

45 – 64

Helical; Potential

Transmembrane

88 – 108

Helical; Potential

Transmembrane

121 – 142

Helical; Potential

Topological domain

143 – 238

Extracellular Potential

Transmembrane

239 – 258

Helical; Potential

Transmembrane

279 – 300

Helical; Potential

Transmembrane

316 – 338

Helical; Potential

Transmembrane

405 – 429

Helical; Potential

Transmembrane

436 – 458

Helical; Potential

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

493

Phosphotyrosine Ref.7

Modified residue

537

Phosphotyrosine Ref.7

Modified residue

562

Phosphoserine Ref.8

Lipidation

S-palmitoyl cysteine Ref.9

Glycosylation

205

N-linked (GlcNAc...) Potential

Glycosylation

215

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

1 – 6

MASTEG → MVS in isoform Glt-1A and isoform Glt-1B.

VSP_006264

Alternative sequence

551 – 572

TLAAN…WKREK → PFPFLDIETCI in isoform Glt-1B.

VSP_006265

Experimental info

Mutagenesis

C → S: Severely impairs glutamate uptake activity. Ref.9

Sequence conflict

D → E in AAA91643. Ref.3

Sequence conflict

G → R in AAA91643. Ref.3

Sequence conflict

112

A → V in AAA91643. Ref.3

Sequence conflict

454

T → I in AAB71737. Ref.4

Sequence conflict

525

K → L in AAB71737. Ref.4

Sequence conflict

572

K → EFD in AAA91643. Ref.3

Sequences

Sequence		Length	Mass (Da)
Isoform Glt-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 13C7C30DED40CA81 Show »	FASTA	572	62,030
10 20 30 40 50 60 MASTEGANNM PKQVEVRMHD SHLSSDEPKH RNLGMRMCDK LGKNLLLSLT VFGVILGAVC 70 80 90 100 110 120 GGLLRLASPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA 130 140 150 160 170 180 MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL 190 200 210 220 230 240 VQACFQQIQT VTKKVLVAPP SEEANTTKAV ISMLNETMNE APEETKIVIK KGLEFKDGMN 250 260 270 280 290 300 VLGLIGFFIA FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII 310 320 330 340 350 360 AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI FQAWITALGT 370 380 390 400 410 420 ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT ALYEAVAAIF IAQMNGVILD 430 440 450 460 470 480 GGQIVTVSLT ATLASIGAAS IPSAGLVTML LILTAVGLPT EDISLLVAVD WLLDRMRTSV 490 500 510 520 530 540 NVVGDSFGAG IVYHLSKSEL DTIDSQHRMQ EDIEMTKTQS IYDDKNHRES NSNQCVYAAH 550 560 570 NSVVIDECKV TLAANGKSAD CSVEEEPWKR EK « Hide
Isoform Glt-1A [UniParc]. Checksum: 930251320261AB8B Show »	FASTA	569	61,771
10 20 30 40 50 60 MVSANNMPKQ VEVRMHDSHL SSDEPKHRNL GMRMCDKLGK NLLLSLTVFG VILGAVCGGL 70 80 90 100 110 120 LRLASPIHPD VVMLIAFPGD ILMRMLKMLI LPLIISSLIT GLSGLDAKAS GRLGTRAMVY 130 140 150 160 170 180 YMSTTIIAAV LGVILVLAIH PGNPKLKKQL GPGKKNDEVS SLDAFLDLIR NLFPENLVQA 190 200 210 220 230 240 CFQQIQTVTK KVLVAPPSEE ANTTKAVISM LNETMNEAPE ETKIVIKKGL EFKDGMNVLG 250 260 270 280 290 300 LIGFFIAFGI AMGKMGEQAK LMVEFFNILN EIVMKLVIMI MWYSPLGIAC LICGKIIAIK 310 320 330 340 350 360 DLEVVARQLG MYMITVIVGL IIHGGIFLPL IYFVVTRKNP FSFFAGIFQA WITALGTASS 370 380 390 400 410 420 AGTLPVTFRC LEDNLGIDKR VTRFVLPVGA TINMDGTALY EAVAAIFIAQ MNGVILDGGQ 430 440 450 460 470 480 IVTVSLTATL ASIGAASIPS AGLVTMLLIL TAVGLPTEDI SLLVAVDWLL DRMRTSVNVV 490 500 510 520 530 540 GDSFGAGIVY HLSKSELDTI DSQHRMQEDI EMTKTQSIYD DKNHRESNSN QCVYAAHNSV 550 560 VIDECKVTLA ANGKSADCSV EEEPWKREK « Hide
Isoform Glt-1B [UniParc]. Checksum: E82305D0F6E4BD91 Show »	FASTA	558	60,617
10 20 30 40 50 60 MVSANNMPKQ VEVRMHDSHL SSDEPKHRNL GMRMCDKLGK NLLLSLTVFG VILGAVCGGL 70 80 90 100 110 120 LRLASPIHPD VVMLIAFPGD ILMRMLKMLI LPLIISSLIT GLSGLDAKAS GRLGTRAMVY 130 140 150 160 170 180 YMSTTIIAAV LGVILVLAIH PGNPKLKKQL GPGKKNDEVS SLDAFLDLIR NLFPENLVQA 190 200 210 220 230 240 CFQQIQTVTK KVLVAPPSEE ANTTKAVISM LNETMNEAPE ETKIVIKKGL EFKDGMNVLG 250 260 270 280 290 300 LIGFFIAFGI AMGKMGEQAK LMVEFFNILN EIVMKLVIMI MWYSPLGIAC LICGKIIAIK 310 320 330 340 350 360 DLEVVARQLG MYMITVIVGL IIHGGIFLPL IYFVVTRKNP FSFFAGIFQA WITALGTASS 370 380 390 400 410 420 AGTLPVTFRC LEDNLGIDKR VTRFVLPVGA TINMDGTALY EAVAAIFIAQ MNGVILDGGQ 430 440 450 460 470 480 IVTVSLTATL ASIGAASIPS AGLVTMLLIL TAVGLPTEDI SLLVAVDWLL DRMRTSVNVV 490 500 510 520 530 540 GDSFGAGIVY HLSKSELDTI DSQHRMQEDI EMTKTQSIYD DKNHRESNSN QCVYAAHNSV 550 VIDECKVPFP FLDIETCI « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." Kirschner M.A., Copeland N.G., Gilbert D.J., Jenkins N.A., Amara S.G. Genomics 24:218-224(1994) [PubMed: 7698742] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Molecular cloning of two glutamate transporter subtypes from mouse brain." Mukainaka Y., Tanaka K., Hagiwara T., Wada K. Biochim. Biophys. Acta 1244:233-237(1995) [PubMed: 7766664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: JCL:ICR. Tissue: Cerebellum.
[3]	"Molecular characterization of a high-affinity mouse glutamate transporter." Sutherland M.L., Delaney T.A., Noebels J.L. Gene 162:271-274(1995) [PubMed: 7557442] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	Peng J.-B., Guo L.-H. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[5]	"Tissue specific variants of glutamate transporter GLT-1." Utsunomiya-Tate N., Endou H., Kanai Y. FEBS Lett. 416:312-316(1997) [PubMed: 9373176] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING. Strain: JCL:ICR. Tissue: Brain and Liver.
[6]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 66-87; 159-173 AND 478-525, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[7]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-493 AND TYR-537, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, MASS SPECTROMETRY. Tissue: Brain cortex.
[9]	"Palmitoylation and function of glial glutamate transporter-1 is reduced in the YAC128 mouse model of Huntington disease." Huang K., Kang M.H., Askew C., Kang R., Sanders S.S., Wan J., Davis N.G., Hayden M.R. Neurobiol. Dis. 40:207-215(2010) [PubMed: 20685337] [Abstract] Cited for: PALMITOYLATION AT CYS-38, MUTAGENESIS OF CYS-38.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11763 mRNA. Translation: AAA77673.1. D43796 mRNA. Translation: BAA07854.1. U24699 mRNA. Translation: AAA91643.1. U75372 mRNA. Translation: AAB71737.1. U75373 mRNA. Translation: AAB71738.1. AB007810 mRNA. Translation: BAA23770.1. AB007811 mRNA. Translation: BAA23771.1. AB007812 mRNA. Translation: BAA23772.1.
IPI	IPI00230289. IPI00230290. IPI00470184.
PIR	A55676. JC4262.
RefSeq	NP_001070982.1. NM_001077514.3. NP_001070983.1. NM_001077515.2. NP_035523.1. NM_011393.2.
UniGene	Mm.267547. Mm.371582.
3D structure databases
ProteinModelPortal	P43006.
SMR	P43006. Positions 46-494.
ModBase	Search...
Protein-protein interaction databases
IntAct	P43006. 5 interactions.
STRING	P43006.
PTM databases
PhosphoSite	P43006.
Proteomic databases
PRIDE	P43006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005220; ENSMUSP00000005220; ENSMUSG00000005089. ENSMUST00000080210; ENSMUSP00000079100; ENSMUSG00000005089. ENSMUST00000111212; ENSMUSP00000106843; ENSMUSG00000005089.
GeneID	20511.
KEGG	mmu:20511.
Organism-specific databases
CTD	6506.
MGI	MGI:101931. Slc1a2.
Phylogenomic databases
HOVERGEN	HBG000080.
OrthoDB	EOG4P8FHT.
PhylomeDB	P43006.
Gene expression databases
ArrayExpress	P43006.
Bgee	P43006.
Genevestigator	P43006.
GermOnline	ENSMUSG00000005089. Mus musculus.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
KO	K05613.
PANTHER	PTHR11958. Na/diCO_symport. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	298703.
SOURCE	Search...

Entry information

Entry name

EAA2_MOUSE

Accession

Primary (citable) accession number: P43006
Secondary accession number(s): O35877, O54686, O54687

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents