P43002 (AT1B2_BUFMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Sodium/potassium-transporting ATPase subunit beta-2 Alternative name(s): Beta-B1 chain Sodium/potassium-dependent ATPase beta-2 subunit
Organism	Bufo marinus (Giant toad) (Cane toad)
Taxonomic identifier	8386 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Hyloidea › Bufonidae › Bufo › Rhinella

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of this glycoprotein is not known. Some specific sequence of the beta subunit can modulate the activation of the Na,K-pump by extracellular potassium ions.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed at a high level in bladder epithelial cells and eye and at a trace level in kidney; it is not detectable in significant amounts in the stomach, colon and small intestine.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Sodium/potassium-transporting ATPase subunit beta-2		PRO_0000219118
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	58 – 299	242	Extracellular Potential
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
	Glycosylation	226	1	N-linked (GlcNAc...) Potential
	Glycosylation	247	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Disulfide bond	130 ↔ 152		By similarity
	Disulfide bond	162 ↔ 178		By similarity
	Disulfide bond	206 ↔ 270		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P43002 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9578295B790C879B	FASTA	299	34,288
	10 20 30 40 50 60 MAALTQKKTC SQMMEEWKEF MWNPRTREFM GRTGSSWALI LLFYVVFYAF LTAVFSLSLW 70 80 90 100 110 120 VMLQTIDEYT PKYADRLANP GLMIRPKMDT TEVVYSTNGM NGTWQAYVDN LNSLLKDYNK 130 140 150 160 170 180 TVQMERGVNC TPGVYNMQED TGDVRNNPKK ACWFFRDVLG DCSGVSDTTY GYQDGKPCVL 190 200 210 220 230 240 IKMNRVINFL PVPIKELSNT SITIKCTAQN NDDLLGSIQY FPSVNNQSLG AIDLMYFPYY 250 260 270 280 290 GNRAQQNYTQ PFVAVKFLNA TKGVDHMVEC RVNAANINNQ DPRDLYQGRV IFTMKIDRL

References

[1]	"Primary sequence and functional expression of a novel beta subunit of the P-ATPase gene family." Jaisser F., Horisberger J., Rossier B.C. Pflugers Arch. 425:446-452(1993) [PubMed: 8134260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Urinary bladder urothelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z25812 mRNA. Translation: CAA81060.1.
PIR	S39267.
3D structure databases
ProteinModelPortal	P43002.
SMR	P43002. Positions 33-78.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050603.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT1B2_BUFMA

Accession

Primary (citable) accession number: P43002

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	October 5, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program