Reviewed, UniProtKB/Swiss-Prot P42975 (BIRA_BACSU)

Names and origin

Protein names

Recommended name:
    Bifunctional protein birA
Including the following 2 domains:
    1- Recommended name:
            Biotin operon repressor
    2- Recommended name:
            Biotin--[acetyl-CoA-carboxylase] synthetase
              EC=6.3.4.15
        Alternative name(s):
            Biotin--protein ligase

Gene names

Name:	birA
Ordered Locus Names:	BSU22440

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins By similarity.
Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
Sequence similarities	Belongs to the biotin--protein ligase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	ATP-binding Biotin DNA-binding Nucleotide-binding
Molecular function	Ligase Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Bifunctional protein birA		PRO_0000064931
Regions
	DNA binding	23 – 42	20	H-T-H motif Potential
Natural variations
	Natural variant	18	1	N → S in strain: W23.
	Natural variant	89	1	H → Q in strain: W23.
	Natural variant	92	1	L → I in strain: W23.
	Natural variant	137	1	V → I in strain: W23.
	Natural variant	169	1	E → A in strain: W23.
	Natural variant	219 – 220	2	PN → SD in strain: W23.
	Natural variant	237	1	A → S in strain: W23.
	Natural variant	311	1	N → Q in strain: W23.
Experimental info
	Mutagenesis	23	1	G → S: Deregulation of biotin synthesis.
	Mutagenesis	38	1	W → R: Deregulation of biotin synthesis.
	Mutagenesis	58	1	G → E: Deregulation of biotin synthesis.

Sequences

	Sequence		Length	Mass (Da)
	P42975-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 69088BB2CAD09221	FASTA	325	36,182
	10 20 30 40 50 60 MRSTLRKDLI ELFSQAGNEF ISGQKISDAL GCSRTAVWKH IEELRKEGYE VEAVRRKGYR 70 80 90 100 110 120 LIKKPGKLSE SEIRFGLKTE VMGQHLIYHD VLSSTQKTAH ELANNNAPEG TLVVADKQTA 130 140 150 160 170 180 GRGRMSRVWH SQEGNGVWMS LILRPDIPLQ KTPQLTLLAA VAVVQGIEEA AGIQTDIKWP 190 200 210 220 230 240 NDILINGKKT VGILTEMQAE EDRVRSVIIG IGINVNQQPN DFPDELKDIA TSLSQAAGEK 250 260 270 280 290 300 IDRAGVIQHI LLCFEKRYRD YMTHGFTPIK LLWESYALGI GTNMRARTLN GTFYGKALGI 310 320 DDEGVLLLET NEGIKKIYSA DIELG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon." Bower S., Perkins J.P., Yocum R.R., Serror P., Sorokin A.V., Rahaim P., Howitt C.L., Prasad N., Ehrlich S.D., Pero J. J. Bacteriol. 177:2572-2575(1995) [PubMed: 7730294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg and W23 / PY79.
[2]	"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome." Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P. Microbiology 142:2005-2016(1996) [PubMed: 8760912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L38424 Genomic DNA. Translation: AAA92879.1. Sequence problems. U20445 Genomic DNA. Translation: AAB60184.1. L47709 Genomic DNA. Translation: AAB38447.1. AL009126 Genomic DNA. Translation: CAB14160.1.
PIR	A69595.
RefSeq	NP_390125.1.
3D structure databases
HSSP	HSSP built from PDB template 1BIB based on UniProtKB P06709.
ModBase	Search...
Genome annotation databases
GeneID	939028.
GenomeReviews	Gene locus BSU22440 in contig AL009126_GR.
KEGG	bsu:BSU22440.
NMPDR	fig|224308.1.peg.2248.
Organism-specific databases
SubtiList	BG11206. birA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P42975.
OMA	GTGIWMS.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2243-MON.
BRENDA	6.3.4.15. 150.
Family and domain databases
InterPro	IPR004408. Biotin_CoA_COase_ligase. IPR003142. BPL_C. IPR004143. BPL_LipA_LipB. IPR013196. HTH_11. IPR004409. HTHbiotin_repres. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR12835. BirA_ligase. 1 hit.
Pfam	PF02237. BPL_C. 1 hit. PF03099. BPL_LipA_LipB. 1 hit. PF08279. HTH_11. 1 hit. [Graphical view]
TIGRFAMs	TIGR00121. birA_ligase. 1 hit. TIGR00122. birA_repr_reg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIRA_BACSU

Accession

Primary (citable) accession number: P42975

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)