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Reviewed, UniProtKB/Swiss-Prot P42974 (DHNA_BACSU)

Last modified November 25, 2008. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADH dehydrogenase
    EC=1.6.99.3
Alternative name(s):
    Alkyl hydroperoxide reductase
Gene names
Name: ahpF
Synonyms: ndh
Ordered Locus Names: BSU40100
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.

Catalytic activity

NADH + acceptor = NAD(+) + reduced acceptor.

Cofactor

Binds 1 FAD per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Peripheral membrane proteinBy similarity.

Sequence similarities

Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509NADH dehydrogenase
PRO_0000166789

Regions

Nucleotide binding210 – 24132FAD By similarity
Nucleotide binding349 – 37931NAD By similarity
Nucleotide binding469 – 47911FAD By similarity
Region1 – ?183183Membrane-binding
Region?184 – 509326Catalytic

Amino acid modifications

Disulfide bond337 ↔ 340Redox-active By similarity

Experimental info

Sequence conflict291G → E AA sequence Ref.4
Sequence conflict2271T → D Ref.3
Sequence conflict3931Y → I Ref.3

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Sequences

Sequence LengthMass (Da)Tools
P42974-1 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 487FFF089CA3A079

FASTA50954,874
        10         20         30         40         50         60 
MVLDANIKAQ LNQYMQLIEN DIVLKVSAGE DDTSKDMLAL VDELASMSSK ISVEKAELNR 

        70         80         90        100        110        120 
TPSFSVNRVG EDTGVTFAGI PLGHEFTSLV LALLQVSGRP PKVDQKVIDQ VKKISGEYHF 

       130        140        150        160        170        180 
ESYISLTCHN CPDVVQALNM MSVLNPNITH TMIDGAAYKA EVESKNIMAV PTVYLNGESF 

       190        200        210        220        230        240 
GSGRMTLEEI LAKMGSGTDA SEFADKEPFD VLVVGGGPAG ASAAIYTARK GIRTGVVAER 

       250        260        270        280        290        300 
FGGQVLDTMS IENFISVKAT EGPKLAASLE EHVKEYDIDV MNLQRAKRLE KKDLFELELE 

       310        320        330        340        350        360 
NGAVLKSKTV ILSTGARWRN VNVPGEQEFK NKGVAYCPHC DGPLFEGKDV AVIGGGNSGI 

       370        380        390        400        410        420 
EAAIDLAGIV NHVTVLEFAP ELKADEVLQK RLYSLPNVTV VKNAQTKEIT GDQSVNGITY 

       430        440        450        460        470        480 
VDRETGEEKH VELQGVFVQI GLVPNTEWLE GTVERNRMGE IIVDKHGATS VPGLFAAGDC 

       490        500 
TDSAYNQIII SMGSGATAAL GAFDYLIRN

« Hide

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References

« Hide 'large scale' references
[1]"36kb sequence between gntZ and trnY of B. subtilis genome."
Kasahara Y., Nakai S., Yoshikawa H., Ogasawara N.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"A Bacillus subtilis bglA gene encoding phospho-beta-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene."
Zhang J., Aronson A.I.
Gene 140:85-90(1994) [PubMed: 8125345] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 137-509.
Strain: 168 / JH642.
[4]"Isolation and characterization of a hydrogen peroxide resistant mutant of Bacillus subtilis."
Hartford O.M., Dowds B.C.A.
Microbiology 140:297-304(1994) [PubMed: 8180695] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-29.
Strain: 168 / YB886.

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Cross-references

Sequence databases

D78193 Genomic DNA. Translation: BAA11269.1.
Z99124 Genomic DNA. Translation: CAB16047.1.
L19710 Genomic DNA. No translation available.
PIRG69583.
RefSeqNP_391890.1.

3D structure databases

HSSPHSSP built from PDB template 1FL2 based on UniProtKB P35340.
ModBaseSearch...

Genome annotation databases

GeneID937717.
GenomeReviewsGene locus BSU40100 in contig AL009126_GR.
KEGGbsu:BSU40100.
NMPDRfig|224308.1.peg.4016.

Organism-specific databases

SubtiListBG11204. ahpF. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP42974.

Enzyme and pathway databases

BioCycBSUB224308:BSU4007-MON.

Family and domain databases

InterProIPR000759. Adrndx_reductase.
IPR012081. Alkyl_hydroperoxide_Rdtase_suF.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR008255. Pyr_nucl-diS_OxRdtase_2_AS.
IPR001327. Pyr_OxRdtase_NAD_bd.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PIRSFPIRSF000238. AhpF. 1 hit.
PRINTSPR00419. ADXRDTASE.
PR00368. FADPNR.
PR00469. PNDRDTASEII.
ProDomPD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03140. AhpF. 1 hit.
PROSITEPS51354. GLUTAREDOXIN_2. 1 hit.
PS00573. PYRIDINE_REDOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDHNA_BACSU
AccessionPrimary (citable) accession number: P42974
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 25, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents