Reviewed,
UniProtKB/Swiss-Prot P42974 (DHNA_BACSU)
Last modified
November 25, 2008.
Version 78.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: NADH dehydrogenase EC=1.6.99.3 Alternative name(s): Alkyl hydroperoxide reductase | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 509 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. |
| Catalytic activity | NADH + acceptor = NAD(+) + reduced acceptor. |
| Cofactor | Binds 1 FAD per subunit By similarity. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Cell membrane; Peripheral membrane proteinBy similarity. |
| Sequence similarities | Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 509 | 509 | NADH dehydrogenase | PRO_0000166789 | |||||||
Regions | |||||||||||
| Nucleotide binding | 210 – 241 | 32 | FAD By similarity | ||||||||
| Nucleotide binding | 349 – 379 | 31 | NAD By similarity | ||||||||
| Nucleotide binding | 469 – 479 | 11 | FAD By similarity | ||||||||
| Region | 1 – ?183 | 183 | Membrane-binding | ||||||||
| Region | ?184 – 509 | 326 | Catalytic | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 337 ↔ 340 | Redox-active By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 29 | 1 | G → E AA sequence Ref.4 | ||||||||
| Sequence conflict | 227 | 1 | T → D Ref.3 | ||||||||
| Sequence conflict | 393 | 1 | Y → I Ref.3 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "36kb sequence between gntZ and trnY of B. subtilis genome." Kasahara Y., Nakai S., Yoshikawa H., Ogasawara N. Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [3] | "A Bacillus subtilis bglA gene encoding phospho-beta-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene." Zhang J., Aronson A.I. Gene 140:85-90(1994) [PubMed: 8125345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 137-509. Strain: 168 / JH642. |
| [4] | "Isolation and characterization of a hydrogen peroxide resistant mutant of Bacillus subtilis." Hartford O.M., Dowds B.C.A. Microbiology 140:297-304(1994) [PubMed: 8180695] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-29. Strain: 168 / YB886. |
Cross-references
Sequence databases | |
|---|---|
| D78193 Genomic DNA. Translation: BAA11269.1. Z99124 Genomic DNA. Translation: CAB16047.1. L19710 Genomic DNA. No translation available. | |
| PIR | G69583. |
| RefSeq | NP_391890.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FL2 based on UniProtKB P35340. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 937717. |
| GenomeReviews | Gene locus BSU40100 in contig AL009126_GR. |
| KEGG | bsu:BSU40100. |
| NMPDR | fig|224308.1.peg.4016. |
Organism-specific databases | |
| SubtiList | BG11204. ahpF. [Micado] |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P42974. |
Enzyme and pathway databases | |
| BioCyc | BSUB224308:BSU4007-MON. |
Family and domain databases | |
| InterPro | IPR000759. Adrndx_reductase. IPR012081. Alkyl_hydroperoxide_Rdtase_suF. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR012335. Thioredoxin_fold. [Graphical view] |
| Gene3D | G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits. |
| Pfam | PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view] |
| PIRSF | PIRSF000238. AhpF. 1 hit. |
| PRINTS | PR00419. ADXRDTASE. PR00368. FADPNR. PR00469. PNDRDTASEII. |
| ProDom | PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR03140. AhpF. 1 hit. |
| PROSITE | PS51354. GLUTAREDOXIN_2. 1 hit. PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DHNA_BACSU | ||||||||
| Accession | Primary (citable) accession number: P42974 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| SIMILARITY comments Index of protein domains and families |

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