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P42973

- BGLA_BACSU

UniProt

P42973 - BGLA_BACSU

Protein

Aryl-phospho-beta-D-glucosidase BglA

Gene

bglA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and BglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells.1 Publication

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei176 – 1761Proton donorSequence Analysis
    Active sitei377 – 3771NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciBSUB:BSU40110-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aryl-phospho-beta-D-glucosidase BglA (EC:3.2.1.86)
    Alternative name(s):
    6-phospho-beta-glucosidase
    Gene namesi
    Name:bglA
    Ordered Locus Names:BSU40110
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU40110. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Aryl-phospho-beta-D-glucosidase BglAPRO_0000063896Add
    BLAST

    Proteomic databases

    PaxDbiP42973.

    Expressioni

    Developmental stagei

    Expressed during stationary-phase and exponential-phase of growth.1 Publication

    Inductioni

    Up-regulated by the aryl-beta-D-glucoside salicin.2 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU40110.

    Structurei

    3D structure databases

    ProteinModelPortaliP42973.
    SMRiP42973. Positions 1-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088631.
    KOiK01223.
    OMAiREGCADY.
    OrthoDBiEOG6F81PM.
    PhylomeDBiP42973.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42973-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNMPKDFLW GGALAAHQFE GGWNQGGKGP SVVDVMTAGA HGVPRKITDT    50
    IEENEFYPNH EAIDFYHRYK EDIALFAEMG LKCLRTSIGW SRIFPKGDEA 100
    EPNEAGLQFY DDVFDELLKH GIEPVITLSH FEMPLHLARE YGGFRNRKVV 150
    DFFVNFAEAC FTRYKDKVKY WMTFNEINNQ MDVNNPLFLW TNSGVVVGEN 200
    ENAKEVMYQT AHHELVASAL AVAKGKDINP EFQIGAMVSH VPIYPFSSNP 250
    EDVMLAEEEM RQRYFFPDVQ VRGYYPSYAL KEFEREGYNI TFEDGDDEIL 300
    RNGTVDYLGF SYYMSTTVKS DVKNDNTGDI VNGGLPNGVE NPYITSSDWG 350
    WAIDPTGLRY TLNRFYDRYQ IPLFIVENGF GAVDTLEEDG KVHDPERIQY 400
    LKSHIEALKK AVTYDGVDLI GYTPWGIIDI VSFTTGEMKK RYGMIYVDRD 450
    NEGNGSMKRY KKDSFEWYKN VIQTNGEEL 479
    Length:479
    Mass (Da):54,841
    Last modified:November 1, 1995 - v1
    Checksum:iFC9FA6131A14257A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19710 Genomic DNA. Translation: AAA22660.1.
    D78193 Genomic DNA. Translation: BAA11270.1.
    AL009126 Genomic DNA. Translation: CAB16048.1.
    PIRiI39953.
    RefSeqiNP_391891.1. NC_000964.3.
    WP_003226994.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB16048; CAB16048; BSU40110.
    GeneIDi937718.
    KEGGibsu:BSU40110.
    PATRICi18980092. VBIBacSub10457_4208.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19710 Genomic DNA. Translation: AAA22660.1 .
    D78193 Genomic DNA. Translation: BAA11270.1 .
    AL009126 Genomic DNA. Translation: CAB16048.1 .
    PIRi I39953.
    RefSeqi NP_391891.1. NC_000964.3.
    WP_003226994.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P42973.
    SMRi P42973. Positions 1-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU40110.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi P42973.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB16048 ; CAB16048 ; BSU40110 .
    GeneIDi 937718.
    KEGGi bsu:BSU40110.
    PATRICi 18980092. VBIBacSub10457_4208.

    Organism-specific databases

    GenoListi BSU40110. [Micado ]

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088631.
    KOi K01223.
    OMAi REGCADY.
    OrthoDBi EOG6F81PM.
    PhylomeDBi P42973.

    Enzyme and pathway databases

    BioCyci BSUB:BSU40110-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Bacillus subtilis bglA gene encoding phospho-beta-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene."
      Zhang J., Aronson A.I.
      Gene 140:85-90(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
      Strain: 168 / JH642.
    2. "Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome."
      Kasahara Y., Nakai S., Ogasawara N.
      DNA Res. 4:155-159(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
      Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
      Arch. Microbiol. 181:60-67(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: 168 / PS832.

    Entry informationi

    Entry nameiBGLA_BACSU
    AccessioniPrimary (citable) accession number: P42973
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3