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Reviewed, UniProtKB/Swiss-Prot P42973 (BGLA_BACSU)

Last modified November 3, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Aryl-phospho-beta-D-glucosidase bglA
    EC=3.2.1.86
Alternative name(s):
    6-phospho-beta-glucosidase
Gene names
Name: bglA
Ordered Locus Names: BSU40110
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the hydrolysis of aryl-phospho-beta-D-glucosides such as 4-methylumbelliferyl-phospho-beta-D-glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. BglA and bglH are the major proteins contributing to hydrolysis of MUG-P by extracts of late-exponential-phase or stationary-phase B.subtilis cells. Ref.4

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Developmental stage

Expressed during stationary-phase and exponential-phase of growth. Ref.4

Induction

Up-regulated by the aryl-beta-D-glucoside salicin. Ref.4 Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function6-phospho-beta-glucosidase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Aryl-phospho-beta-D-glucosidase bglA
PRO_0000063896

Sites

Active site1761Proton donor Potential
Active site3771Nucleophile By similarity

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Sequences

Sequence LengthMass (Da)Tools
P42973-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: FC9FA6131A14257A

FASTA47954,841
        10         20         30         40         50         60 
MGNMPKDFLW GGALAAHQFE GGWNQGGKGP SVVDVMTAGA HGVPRKITDT IEENEFYPNH 

        70         80         90        100        110        120 
EAIDFYHRYK EDIALFAEMG LKCLRTSIGW SRIFPKGDEA EPNEAGLQFY DDVFDELLKH 

       130        140        150        160        170        180 
GIEPVITLSH FEMPLHLARE YGGFRNRKVV DFFVNFAEAC FTRYKDKVKY WMTFNEINNQ 

       190        200        210        220        230        240 
MDVNNPLFLW TNSGVVVGEN ENAKEVMYQT AHHELVASAL AVAKGKDINP EFQIGAMVSH 

       250        260        270        280        290        300 
VPIYPFSSNP EDVMLAEEEM RQRYFFPDVQ VRGYYPSYAL KEFEREGYNI TFEDGDDEIL 

       310        320        330        340        350        360 
RNGTVDYLGF SYYMSTTVKS DVKNDNTGDI VNGGLPNGVE NPYITSSDWG WAIDPTGLRY 

       370        380        390        400        410        420 
TLNRFYDRYQ IPLFIVENGF GAVDTLEEDG KVHDPERIQY LKSHIEALKK AVTYDGVDLI 

       430        440        450        460        470 
GYTPWGIIDI VSFTTGEMKK RYGMIYVDRD NEGNGSMKRY KKDSFEWYKN VIQTNGEEL

« Hide

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References

« Hide 'large scale' references
[1]"A Bacillus subtilis bglA gene encoding phospho-beta-glucosidase is inducible and closely linked to a NADH dehydrogenase-encoding gene."
Zhang J., Aronson A.I.
Gene 140:85-90(1994) [PubMed: 8125345] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: 168 / JH642.
[2]"Sequence analysis of the 36-kb region between gntZ and trnY genes of Bacillus subtilis genome."
Kasahara Y., Nakai S., Ogasawara N.
DNA Res. 4:155-159(1997) [PubMed: 9205843] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis."
Setlow B., Cabrera-Hernandez A., Cabrera-Martinez R.M., Setlow P.
Arch. Microbiol. 181:60-67(2004) [PubMed: 14652714] [Abstract]
Cited for: FUNCTION AS AN ARYL-PHOSPHO-BETA-D-GLUCOSIDASE, DEVELOPMENTAL STAGE, INDUCTION.
Strain: 168 / PS832.

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Cross-references

Sequence databases

L19710 Genomic DNA. Translation: AAA22660.1.
D78193 Genomic DNA. Translation: BAA11270.1.
AL009126 Genomic DNA. Translation: CAB16048.1.
PIRI39953.
RefSeqNP_391891.1.

3D structure databases

HSSPHSSP built from PDB template 1OD0 based on UniProtKB Q08638.
ModBaseSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Genome annotation databases

GeneID937718.
GenomeReviewsGene locus BSU40110 in contig AL009126_GR.
KEGGbsu:BSU40110.
NMPDRfig|224308.1.peg.4017.

Organism-specific databases

SubtiListBG11203. bglA. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP42973.
OMAIGHKINP.

Enzyme and pathway databases

BioCycBSUB224308:BSU4008-MON.
BRENDA3.2.1.86. 150.

Family and domain databases

InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR10353. Glyco_hydro_1. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameBGLA_BACSU
AccessionPrimary (citable) accession number: P42973
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents