P42957 (MTLD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannitol-1-phosphate 5-dehydrogenase
EC=1.1.1.17

Gene names

Name:	mtlD
Synonyms:	mtlB
Ordered Locus Names:	BSU03990

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH. HAMAP MF_00196
Sequence similarities	Belongs to the mannitol dehydrogenase family.
Sequence caution	The sequence BAA07351.1 differs from that shown. Reason: Frameshift at position 346. The sequence BAA09030.1 differs from that shown. Reason: Frameshift at position 346.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Mannitol-1-phosphate 5-dehydrogenase HAMAP MF_00196		PRO_0000170698
Regions
	Nucleotide binding	3 – 14	12	NAD By similarity
Experimental info
	Sequence conflict	149	1	V → L in BAA07351. Ref.1
	Sequence conflict	149	1	V → L in BAA09030. Ref.2
	Sequence conflict	273 – 302	30	KIIGR…LGEND → NQRSLLKSFHFGRCDPRSEV TSQKTGRKC in BAA07351. Ref.1
	Sequence conflict	273 – 302	30	KIIGR…LGEND → NQRSLLKSFHFGRCDPRSEV TSQKTGRKC in BAA09030. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P42957 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: 533D9B1CA8E221DA	FASTA	373	41,118
	10 20 30 40 50 60 MIALHFGAGN IGRGFIGALL HHSGYDVVFA DVNETMVSLL NEKKEYTVEL AEEGRSSEII 70 80 90 100 110 120 GPVSAINSGS QTEELYRLMN EAALITTAVG PNVLKLIAPS IAEGLRRRNT ANTLNIIACE 130 140 150 160 170 180 NMIGGSSFLK KEIYSHLTEA EQKSVSETVG FPNSAVDRIV PIQHHEDPLK VSVEPFFEWV 190 200 210 220 230 240 IDESGFKGKT PVINGALFVD DLTPYIERKL FTVNTGHAVT AYVGYQRGLK TVKEAIDHPE 250 260 270 280 290 300 IRRVVHSALL ETGDYLVKSY GFKQTEHEQY IKKIIGRFEN PFISDDVTRV ARSPLRKLGE 310 320 330 340 350 360 NDRLVGPAKK IKEPNALAEG IAAALRFDFT GDPEAVELQA LIEEKGYSGV LQEVCGIQSH 370 EPLHAIILKK LNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Determination of a 17,484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs." Akagawa E., Kurita K., Sugawara T., Nakamura K., Kasahara Y., Ogasawara N., Yamane K. Microbiology 141:3241-3245(1995) [PubMed: 8574415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	Yamane K. Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes." Yamane K., Kumano M., Kurita K. Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[4]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[5]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 149; 273-302 AND C-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38161 Genomic DNA. Translation: BAA07351.1. Frameshift. D50453 Genomic DNA. Translation: BAA09030.1. Frameshift. AL009126 Genomic DNA. Translation: CAB12207.2.
PIR	I39888.
RefSeq	NP_388281.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P42957.
SMR	P42957. Positions 1-373.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003900; EBBACP00000003900; EBBACG00000003892.
GeneID	939968.
GenomeReviews	Gene locus BSU03990 in contig AL009126_GR.
KEGG	bsu:BSU03990.
NMPDR	fig|224308.1.peg.400.
PATRIC	18972374. VBIBacSub10457_0415.
Organism-specific databases
GenoList	BSU03990. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000001216.
HOGENOM	HBG568825.
PhylomeDB	P42957.
ProtClustDB	PRK02318.
Enzyme and pathway databases
BioCyc	BSUB:BSU03990-MONOMER.
Family and domain databases
HAMAP	MF_00196. Mannitol_dehydrog. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR023028. Mannitol_1_phos_5_DH. IPR000669. Mannitol_DH. IPR013118. Mannitol_DH_C. IPR023027. Mannitol_DH_CS. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00009.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_BACSU

Accession

Primary (citable) accession number: P42957

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program