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P42956 (PTMCB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PTS system mannitol-specific EIICB component
Alternative name(s):
EIICB-Mtl
Short name=EII-Mtl

Including the following 2 domains:

  1. Mannitol permease IIC component
    Alternative name(s):
    PTS system mannitol-specific EIIC component
  2. Mannitol-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system mannitol-specific EIIB component
Gene names
Name:mtlA
Ordered Locus Names:BSU03981
ORF Names:BSU03980
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport By similarity.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-2 domain.

Contains 1 PTS EIIC type-2 domain.

Caution

Was originally thought to be a longer ORF that encodes what is now known to be mtlA and mtlF.

Sequence caution

The sequence BAA07350.1 differs from that shown. Reason: Frameshift at position 478.

The sequence BAA09029.1 differs from that shown. Reason: Frameshift at position 478.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478PTS system mannitol-specific EIICB component
PRO_0000186610

Regions

Transmembrane30 – 5021Helical; Potential
Transmembrane54 – 7421Helical; Potential
Transmembrane90 – 11021Helical; Potential
Transmembrane141 – 16121Helical; Potential
Transmembrane167 – 18721Helical; Potential
Transmembrane189 – 20921Helical; Potential
Transmembrane219 – 23921Helical; Potential
Transmembrane251 – 27121Helical; Potential
Transmembrane273 – 29321Helical; Potential
Transmembrane319 – 33921Helical; Potential
Domain18 – 347330PTS EIIC type-2
Domain390 – 47889PTS EIIB type-2

Sites

Active site3961Phosphocysteine intermediate; for EIIB activity By similarity

Experimental info

Sequence conflict133 – 1353EML → GIR in BAA07350. Ref.1
Sequence conflict1331E → G in BAA09029. Ref.2
Sequence conflict1871V → L in BAA07350. Ref.1
Sequence conflict1871V → L in BAA09029. Ref.2
Sequence conflict2341L → F in BAA07350. Ref.1
Sequence conflict2341L → F in BAA09029. Ref.2
Sequence conflict274 – 29017ALILA…GLLTF → GPDSRSHCRRSKRTLNI in BAA07350. Ref.1
Sequence conflict274 – 29017ALILA…GLLTF → GPDSRSHCRRSKRTLNI in BAA09029. Ref.2
Sequence conflict380 – 41536EASEL…RNKVK → KRLSCLLKARTKLSFRVIRD GIKCHGGIHLKKQSE in BAA07350. Ref.1
Sequence conflict380 – 41536EASEL…RNKVK → KRLSCLLKARTKLSFRVIRD GIKCHGGIHLKKQSE in BAA09029. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P42956 [UniParc].

Last modified June 16, 2009. Version 4.
Checksum: 368BF52A316CC235

FASTA47850,185
        10         20         30         40         50         60 
MQQQEQQQGG MKVKVQRFGS YLSGMIMPNI GAFIAWGIIT ALFIPAGWFP NEQLNTLVSP 

        70         80         90        100        110        120 
MITYLLPLLI AYTGGKMIYD HRGGVVGATA AIGVIVGSDI PMFLGAMIMG PLGGYLIKQT 

       130        140        150        160        170        180 
DKLFKDKVKQ GFEMLINNFT AGIVGAALTI LAFYAIGPVV LTLNKLLAAG VEVIVHANLL 

       190        200        210        220        230        240 
PVASVFVEPA KVLFLNNAIN HGILSPIGIE QASQTGKSIL FLVEANPGPG LGILLAYMFF 

       250        260        270        280        290        300 
GKGSSKSTAP GAAIIHFFGG IHEIYFPYIL MKPALILAAI AGGASGLLTF TIFNAGLVAA 

       310        320        330        340        350        360 
ASPGSIIALM AMTPRGGYFG VLAGVLVAAA VSFIVSAVIL KSSKASEEDL AAATEKMQSM 

       370        380        390        400        410        420 
KGKKSQAAAA LEAEQAKAEE ASELSPESVN KIIFACDAGM GSSAMGASIL RNKVKKAELD 

       430        440        450        460        470 
ISVTNTAINN LPSDADIVIT HKDLTDRAKA KLPNATHISV DNFLNSPKYD ELIEKLKK

« Hide

References

« Hide 'large scale' references
[1]"Determination of a 17,484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs."
Akagawa E., Kurita K., Sugawara T., Nakamura K., Kasahara Y., Ogasawara N., Yamane K.
Microbiology 141:3241-3245(1995) [PubMed: 8574415] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The 25 degrees-36 degrees region of the Bacillus subtilis chromosome: determination of the sequence of a 146 kb segment and identification of 113 genes."
Yamane K., Kumano M., Kurita K.
Microbiology 142:3047-3056(1996) [PubMed: 8969502] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 133; 187; 234; 274-290; 380-415 AND C-TERMINUS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D38161 Genomic DNA. Translation: BAA07350.1. Frameshift.
D50453 Genomic DNA. Translation: BAA09029.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12206.2.
PIRE69661.
RefSeqNP_388280.2. NC_000964.3.

3D structure databases

ProteinModelPortalP42956.
SMRP42956. Positions 389-476.
ModBaseSearch...

Protein family/group databases

TCDB4.A.2.1.5. PTS fructose-mannitol (Fru) family.

PTM databases

PhosSiteP42956.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000002854; EBBACP00000002854; EBBACG00000002849.
GeneID938263.
GenomeReviewsGene locus BSU03981 in contig AL009126_GR.
KEGGbsu:BSU03981.
PATRIC18972370. VBIBacSub10457_0413.

Organism-specific databases

GenoListBSU03981. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000000035.
HOGENOMHBG296680.
PhylomeDBP42956.
ProtClustDBCLSK2459953.

Enzyme and pathway databases

BioCycBSUB:BSU03981-MONOMER.

Family and domain databases

InterProIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004718. PTS_IIC_mtl.
[Graphical view]
KOK02799.
K02800.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00851. MtlA. 1 hit.
PROSITEPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTMCB_BACSU
AccessionPrimary (citable) accession number: P42956
Secondary accession number(s): P94435
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: January 25, 2012
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents