Reviewed, UniProtKB/Swiss-Prot P42951 (LSB6_YEAST)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 4-kinase LSB6
      Short name=PtdIns-4-kinase
      Short name=PI4-kinase
    EC=2.7.1.67
Alternative name(s):
    LAS seventeen-binding protein 6
      Short name=LAS17-binding protein 6

Gene names

Name:	LSB6
Ordered Locus Names:	YJL100W
ORF Names:	J0834

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in endocytic and/or exocytic pathways. Ref.4
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. Ref.4 Ref.3
Cofactor	Magnesium or manganese. Ref.3
Subunit structure	Interacts with LAS17. Ref.4
Subcellular location	Cell membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Note: Located at both the plasma and vacuolar membrane. Ref.4 Ref.3
Miscellaneous	Present with 56 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Vacuole
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Ref.3 Ref.4 Inferred from direct assay. Source: SGD
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole membrane Ref.3 Ref.4 Inferred from direct assay. Source: SGD plasma membrane Ref.3 Ref.4 Inferred from direct assay. Source: SGD
Molecular function	1-phosphatidylinositol 4-kinase activity Ref.3 Ref.4 Inferred from direct assay. Source: SGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Phosphatidylinositol 4-kinase LSB6		PRO_0000088850
Regions
	Domain	170 – 507	338	PI3K/PI4K

Sequences

	Sequence		Length	Mass (Da)
	P42951-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9969EC2488A72456	FASTA	607	70,217
	10 20 30 40 50 60 MSNEAYQHDH TVNPHQKIVV NSYDWLQFRD EQDHCKSKNP ITHASPGVGS NAQNSDIAEA 70 80 90 100 110 120 PQVFHPSYQS LVNVPSESPR PDQTSGSNPA VGLLHNAEDK ASGQEEEGSQ YEIQYSVFRP 130 140 150 160 170 180 LHAYPTKGLA YEQLRRKEEQ EQRENFNHLV SDCIEAVETF GRELERIQTG SSGSYFVYGT 190 200 210 220 230 240 RADESVPVGV FKPKDEEPYG PFSPKWTKWA HRTFFPCLFG RSCLIPNLGY ICESAASLLD 250 260 270 280 290 300 RRLETHLVPY TDTASIESFN FYDNRKKWVL GYNLQKKKQK KLGSFQLFLK EYINADEFFH 310 320 330 340 350 360 KYPLPGMYSD VKHSFHRKSS GEDINHKPET TRNLTDETEP SKQINSSPIS TESEENSKFE 370 380 390 400 410 420 WTESSLSQFR LELEKLIILD YIMRNTDRGL DNWMVKLIKL SNNKWRLKLA AIDNGLSFPW 430 440 450 460 470 480 KHPDEWRLYP YGWLYLPLQL LAKPFSEQMR SHFLPILTST NWWEESYQEF LALFSRDQDF 490 500 510 520 530 540 NVRMWKKQWA VLKGQAFNVV ETLKDPRQGP LELVRRTRCQ VIDEKMQVPC CPPPVSIFKN 550 560 570 580 590 600 AIDEPIGSYS TSPMVLPSTP STIPFHAHNQ SNSNPVYYDS TLHPFANKTV IAERLQIVNS TPVFTWC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)." Rasmussen S.W. Yeast 11:873-883(1995) [PubMed: 7483851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The Saccharomyces cerevisiae LSB6 gene encodes phosphatidylinositol 4-kinase activity." Han G.-S., Audhya A., Markley D.J., Emr S.D., Carman G.M. J. Biol. Chem. 277:47709-47718(2002) [PubMed: 12361950] [Abstract] Cited for: CATALYTIC ACTIVITY, COFACTOR, SUBCELLULAR LOCATION.
[4]	"Saccharomyces cerevisiae contains a Type II phosphoinositide 4-kinase." Shelton S.N., Barylko B., Binns D.D., Horazdovsky B.F., Albanesi J.P., Goodman J.M. Biochem. J. 371:533-540(2003) [PubMed: 12523934] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH LAS17, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85021 Genomic DNA. Translation: CAA59394.1. Z49375 Genomic DNA. Translation: CAA89395.1.
PIR	S53387.
RefSeq	NP_012435.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4035N.
IntAct	P42951. 4 interactions.
STRING	P42951.
Genome annotation databases
Ensembl	YJL100W; YJL100W; YJL100W; Saccharomyces cerevisiae. [Genome view]
GeneID	853345.
GenomeReviews	Gene locus YJL100W in contig Y13136_GR.
KEGG	sce:YJL100W.
NMPDR	fig|4932.3.peg.3404.
Organism-specific databases
CYGD	YJL100w.
SGD	S000003636. LSB6.
Phylogenomic databases
HOGENOM	P42951.
OMA	IILDYIM.
Enzyme and pathway databases
BRENDA	2.7.1.67. 250.
Gene expression databases
ArrayExpress	P42951.
Genevestigator	P42951.
GermOnline	YJL100W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. [Graphical view]
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973738.

Entry information

Entry name

LSB6_YEAST

Accession

Primary (citable) accession number: P42951

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)