Mitochondrial import inner membrane translocase subunit TIM16 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P42949 (TIM16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM16

Alternative name(s):
Presequence translocated-associated motor subunit PAM16

Gene names

Name:	PAM16
Synonyms:	TIM16
Ordered Locus Names:	YJL104W
ORF Names:	J0822

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation. Ref.6 Ref.7 Ref.8
Subunit structure	Homodimer and heterodimer with PAM18. Homodimerization may not be relevant in vivo, while heterodimerization is essential for activity regulation of mtHSP70. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18. Interacts with MDJ2. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein Ref.7 Ref.8.
Domain	The J-like region, although related to the J domain does not stimulate ATPase activity of mtHSP70. It nevertheless mediates the heterodimerization with the J domain of PAM18 and is therefore essential for PAM complex function. Ref.11
Miscellaneous	Present with 3180 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the TIM16/PAM16 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into mitochondrial matrix Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	presequence translocase-associated import motor Inferred from direct assay Ref.8 Ref.7. Source: SGD
Molecular_function	protein domain specific binding Inferred from direct assay Ref.11. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 149

149

Mitochondrial import inner membrane translocase subunit TIM16

PRO_0000214098

Regions

Region

57 – 113

J-like

Experimental info

Mutagenesis

88 – 90

DKE → HPD: Does not confer ability to stimulate the ATPase activity of mtHSP70. Ref.6

Sequence conflict

114

Q → H in AAT92626. Ref.4

Secondary structure

149

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P42949 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 42BB69D746264B47
Show »

FASTA

149

16,216

        10         20         30         40         50         60 
MAHRAFIQVI ITGTQVFGKA FAEAYRQAAS QSVKQGATNA SRRGTGKGEY GGITLDESCK 

        70         80         90        100        110        120 
ILNIEESKGD LNMDKINNRF NYLFEVNDKE KGGSFYLQSK VYRAAERLKW ELAQREKNAK 

       130        140 
AKAGDASTAK PPPNSTNSSG ADNSASSNQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)." Rasmussen S.W. Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18." Li Y., Dudek J., Guiard B., Pfanner N., Rehling P., Voos W. J. Biol. Chem. 279:38047-38054(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PAM18, MUTAGENESIS OF 88-ASP--GLU-90.
[7]	"Pam16 has an essential role in the mitochondrial protein import motor." Frazier A.E., Dudek J., Guiard B., Voos W., Li Y., Lind M., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Bilanchone V., Cumsky M.G., Truscott K.N., Pfanner N., Rehling P. Nat. Struct. Mol. Biol. 11:226-233(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PAM18.
[8]	"The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase." Kozany C., Mokranjac D., Sichting M., Neupert W., Hell K. Nat. Struct. Mol. Biol. 11:234-241(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
[9]	"The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2." Mokranjac D., Sichting M., Popov-Celeketic D., Berg A., Hell K., Neupert W. J. Biol. Chem. 280:31608-31614(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MDJ2.
[10]	"Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor." van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A., Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P. Mol. Cell. Biol. 25:7449-7458(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE PAM COMPLEX WITH PAM17; PAM18; TIM44; SSC1 AND MGE1.
[11]	"Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane." D'Silva P.R., Schilke B., Walter W., Craig E.A. Proc. Natl. Acad. Sci. U.S.A. 102:12419-12424(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT, DOMAIN J, INTERACTION WITH PAM18.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X85021 Genomic DNA. Translation: CAA59390.1.
Z49379 Genomic DNA. Translation: CAA89399.1.
AY692607 Genomic DNA. Translation: AAT92626.1.
BK006943 Genomic DNA. Translation: DAA08696.1.

PIR

S53383.

RefSeq

NP_012431.1. NM_001181537.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GUZ	X-ray	2.00	B/D/F/H/J/L/N/P	54-117	[»]

ProteinModelPortal

P42949.

SMR

P42949. Positions 53-117.

ModBase

Search...

Protein-protein interaction databases

IntAct

P42949. 4 interactions.

MINT

MINT-3374752.

STRING

4932.YJL104W.

Protein family/group databases

TCDB

3.A.8.1.1. mitochondrial protein translocase (MPT) family.

Proteomic databases

PaxDb

P42949.

PeptideAtlas

P42949.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJL104W; YJL104W; YJL104W.

GeneID

853340.

KEGG

sce:YJL104W.

Organism-specific databases

CYGD

YJL104w.

SGD

S000003640. PAM16.

Phylogenomic databases

eggNOG

NOG309665.

GeneTree

ENSGT00390000012037.

HOGENOM

HOG000180095.

OMA

EHLFAIN.

OrthoDB

EOG4V46JF.

Enzyme and pathway databases

BioCyc

YEAST:G3O-31558-MONOMER.

Reactome

REACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

Genevestigator

P42949.

GermOnline

YJL104W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005341. Tim16.
[Graphical view]

PANTHER

PTHR12388. PTHR12388. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P42949.

NextBio

973724.

Entry information

Entry name

TIM16_YEAST

Accession

Primary (citable) accession number: P42949
Secondary accession number(s): D6VW80, Q6B2X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents