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Protein

Mitochondrial import inner membrane translocase subunit TIM16

Gene

PAM16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.3 Publications

GO - Molecular functioni

  • protein domain specific binding Source: SGD

GO - Biological processi

  • protein import into mitochondrial matrix Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31558-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM16
Alternative name(s):
Presequence translocated-associated motor subunit PAM16
Gene namesi
Name:PAM16
Synonyms:TIM16
Ordered Locus Names:YJL104W
ORF Names:J0822
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL104W.
SGDiS000003640. PAM16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi88 – 903DKE → HPD: Does not confer ability to stimulate the ATPase activity of mtHSP70. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Mitochondrial import inner membrane translocase subunit TIM16PRO_0000214098Add
BLAST

Proteomic databases

MaxQBiP42949.

Interactioni

Subunit structurei

Homodimer and heterodimer with PAM18. Homodimerization may not be relevant in vivo, while heterodimerization is essential for activity regulation of mtHSP70. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18. Interacts with MDJ2.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAM18Q079145EBI-26019,EBI-31963
TIM17P395154EBI-26019,EBI-9127
TIM23P328976EBI-26019,EBI-9136
TIM44Q018523EBI-26019,EBI-9141

GO - Molecular functioni

  • protein domain specific binding Source: SGD

Protein-protein interaction databases

BioGridi33652. 73 interactions.
IntActiP42949. 10 interactions.
MINTiMINT-3374752.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi55 – 617Combined sources
Helixi66 – 683Combined sources
Helixi73 – 8614Combined sources
Helixi89 – 913Combined sources
Helixi95 – 11622Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GUZX-ray2.00B/D/F/H/J/L/N/P54-117[»]
ProteinModelPortaliP42949.
SMRiP42949. Positions 53-117.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42949.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 11357J-likeAdd
BLAST

Domaini

The J-like region, although related to the J domain does not stimulate ATPase activity of mtHSP70. It nevertheless mediates the heterodimerization with the J domain of PAM18 and is therefore essential for PAM complex function.1 Publication

Sequence similaritiesi

Belongs to the TIM16/PAM16 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000012037.
HOGENOMiHOG000180095.
InParanoidiP42949.
KOiK17805.
OMAiWELAQRE.
OrthoDBiEOG7FFN55.

Family and domain databases

InterProiIPR005341. Tim16.
[Graphical view]
PANTHERiPTHR12388. PTHR12388. 1 hit.

Sequencei

Sequence statusi: Complete.

P42949-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRAFIQVI ITGTQVFGKA FAEAYRQAAS QSVKQGATNA SRRGTGKGEY
60 70 80 90 100
GGITLDESCK ILNIEESKGD LNMDKINNRF NYLFEVNDKE KGGSFYLQSK
110 120 130 140
VYRAAERLKW ELAQREKNAK AKAGDASTAK PPPNSTNSSG ADNSASSNQ
Length:149
Mass (Da):16,216
Last modified:November 1, 1995 - v1
Checksum:i42BB69D746264B47
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141Q → H in AAT92626 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85021 Genomic DNA. Translation: CAA59390.1.
Z49379 Genomic DNA. Translation: CAA89399.1.
AY692607 Genomic DNA. Translation: AAT92626.1.
BK006943 Genomic DNA. Translation: DAA08696.1.
PIRiS53383.
RefSeqiNP_012431.1. NM_001181537.1.

Genome annotation databases

EnsemblFungiiYJL104W; YJL104W; YJL104W.
GeneIDi853340.
KEGGisce:YJL104W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85021 Genomic DNA. Translation: CAA59390.1.
Z49379 Genomic DNA. Translation: CAA89399.1.
AY692607 Genomic DNA. Translation: AAT92626.1.
BK006943 Genomic DNA. Translation: DAA08696.1.
PIRiS53383.
RefSeqiNP_012431.1. NM_001181537.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GUZX-ray2.00B/D/F/H/J/L/N/P54-117[»]
ProteinModelPortaliP42949.
SMRiP42949. Positions 53-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33652. 73 interactions.
IntActiP42949. 10 interactions.
MINTiMINT-3374752.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiP42949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL104W; YJL104W; YJL104W.
GeneIDi853340.
KEGGisce:YJL104W.

Organism-specific databases

EuPathDBiFungiDB:YJL104W.
SGDiS000003640. PAM16.

Phylogenomic databases

GeneTreeiENSGT00390000012037.
HOGENOMiHOG000180095.
InParanoidiP42949.
KOiK17805.
OMAiWELAQRE.
OrthoDBiEOG7FFN55.

Enzyme and pathway databases

BioCyciYEAST:G3O-31558-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

EvolutionaryTraceiP42949.
PROiP42949.

Family and domain databases

InterProiIPR005341. Tim16.
[Graphical view]
PANTHERiPTHR12388. PTHR12388. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
    Rasmussen S.W.
    Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The presequence translocase-associated protein import motor of mitochondria. Pam16 functions in an antagonistic manner to Pam18."
    Li Y., Dudek J., Guiard B., Pfanner N., Rehling P., Voos W.
    J. Biol. Chem. 279:38047-38054(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PAM18, MUTAGENESIS OF 88-ASP--GLU-90.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PAM18.
  8. "The J domain-related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase."
    Kozany C., Mokranjac D., Sichting M., Neupert W., Hell K.
    Nat. Struct. Mol. Biol. 11:234-241(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE PAM COMPLEX, FUNCTION, SUBCELLULAR LOCATION.
  9. "The import motor of the yeast mitochondrial TIM23 preprotein translocase contains two different J proteins, Tim14 and Mdj2."
    Mokranjac D., Sichting M., Popov-Celeketic D., Berg A., Hell K., Neupert W.
    J. Biol. Chem. 280:31608-31614(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MDJ2.
  10. "Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor."
    van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A., Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P.
    Mol. Cell. Biol. 25:7449-7458(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PAM COMPLEX WITH PAM17; PAM18; TIM44; SSC1 AND MGE1.
  11. "Role of Pam16's degenerate J domain in protein import across the mitochondrial inner membrane."
    D'Silva P.R., Schilke B., Walter W., Craig E.A.
    Proc. Natl. Acad. Sci. U.S.A. 102:12419-12424(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, DOMAIN J, INTERACTION WITH PAM18.

Entry informationi

Entry nameiTIM16_YEAST
AccessioniPrimary (citable) accession number: P42949
Secondary accession number(s): D6VW80, Q6B2X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.