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P42945 (UTP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U3 small nucleolar RNA-associated protein 10

Short name=U3 snoRNA-associated protein 10
Alternative name(s):
U three protein 10
U3 protein 10 required for transcription
t-UTP10
Gene names
Name:UTP10
Ordered Locus Names:YJL109C
ORF Names:J0808
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1769 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Involved in ribosome biosynthesis. Ref.4 Ref.8 Ref.10 Ref.11

Subunit structure

Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs. Ref.4 Ref.8 Ref.11

Subcellular location

Nucleusnucleolus. Mitochondrion. Note: Associated with ribosomal chromatin, even in the absence of transcription. Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.11

Miscellaneous

Present with 18200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the HEATR1/UTP10 family.

Contains 1 HEAT repeat.

Ontologies

Keywords
   Biological processRibosome biogenesis
rRNA processing
Transcription
   Cellular componentMitochondrion
Nucleus
   Molecular functionRibonucleoprotein
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from mutant phenotype Ref.11. Source: SGD

endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from mutant phenotype Ref.11. Source: SGD

endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from mutant phenotype Ref.11. Source: SGD

maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Inferred from mutant phenotype Ref.4. Source: SGD

positive regulation of transcription from RNA polymerase I promoter

Inferred from mutant phenotype Ref.8. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_component90S preribosome

Inferred from direct assay Ref.11. Source: SGD

mitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleolus

Inferred from direct assay Ref.4PubMed 12150911Ref.11. Source: SGD

preribosome, small subunit precursor

Inferred from direct assay Ref.11. Source: SGD

rDNA heterochromatin

Inferred from direct assay Ref.8. Source: SGD

small-subunit processome

Inferred from direct assay Ref.4. Source: SGD

t-UTP complex

Inferred from direct assay PubMed 17515605. Source: SGD

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 12150911PubMed 16429126PubMed 18467557. Source: IntAct

snoRNA binding

Inferred from physical interaction Ref.4. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UTP4Q066794EBI-1884,EBI-35712

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 17691768U3 small nucleolar RNA-associated protein 10
PRO_0000186207

Regions

Repeat1729 – 176739HEAT

Amino acid modifications

Modified residue21N-acetylserine Ref.13

Sequences

Sequence LengthMass (Da)Tools
P42945 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 064480D1D249B241

FASTA1,769200,082
        10         20         30         40         50         60 
MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS 

        70         80         90        100        110        120 
QIEPKFAIFS RTLFSESSIS LDRNVQTKEE IKDLDNAINA YLLLASSKWY LAPTLHATEW 

       130        140        150        160        170        180 
LVRRFQIHVK NTEMLLLSTL NYYQTPVFKR ILSIIKLPPL FNCLSNFVRS EKPPTALTMI 

       190        200        210        220        230        240 
KLFNDMDFLK LYTSYLDQCI KHNATYTNQL LFTTCCFINV VAFNSNNDEK LNQLVPILLE 

       250        260        270        280        290        300 
ISAKLLASKS KDCQIAAHTI LVVFATALPL KKTIILAAME TILSNLDAKE AKHSALLTIC 

       310        320        330        340        350        360 
KLFQTLKGQG NVDQLPSKIF KLFDSKFDTV SILTFLDKED KPVCDKFITS YTRSIARYDR 

       370        380        390        400        410        420 
SKLNIILSLL KKIRLERYEV RLIITDLIYL SEILEDKSQL VELFEYFISI NEDLVLKCLK 

       430        440        450        460        470        480 
SLGLTGELFE IRLTTSLFTN ADVNTDIVKQ LSDPVETTKK DTASFQTFLD KHSELINTTN 

       490        500        510        520        530        540 
VSMLTETGER YKKVLSLFTE AIGKGYKASS FLTSFFTTLE SRITFLLRVT ISPAAPTALK 

       550        560        570        580        590        600 
LISLNNIAKY INSIEKEVNI FTLVPCLICA LRDASIKVRT GVKKILSLIA KRPSTKHYFL 

       610        620        630        640        650        660 
SDKLYGENVT IPMLNPKDSE AWLSGFLNEY VTENYDISRI LTPKRNEKVF LMFWANQALL 

       670        680        690        700        710        720 
IPSPYAKTVL LDNLNKSPTY ASSYSSLFEE FISHYLENRS SWEKSCIANK TNFEHFERSL 

       730        740        750        760        770        780 
VNLVSPKEKQ SFMIDFVLSA LNSDYEQLAN IAAERLISIF ASLNNAQKLK IVQNIVDSSS 

       790        800        810        820        830        840 
NVESSYDTVG VLQSLPLDSD IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV 

       850        860        870        880        890        900 
SQLAELHLRK LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGLP VLYAQETLIS 

       910        920        930        940        950        960 
CTLNTITYLK EHGCTELTNV RADILVSAIR NSASPQVQNK LLLVIGSLAT LSSEVILHSV 

       970        980        990       1000       1010       1020 
MPIFTFMGAH SIRQDDEFTT KVVERTILTV VPALIKNSKG NEKEEMEFLL LSFTTALQHV 

      1030       1040       1050       1060       1070       1080 
PRHRRVKLFS TLIKTLDPVK ALGSFLFLIA QQYSSALVNF KIGEARILIE FIKALLVDLH 

      1090       1100       1110       1120       1130       1140 
VNEELSGLND LLDIIKLLTS SKSSSEKKKS LESRVLFSNG VLNFSESEFL TFMNNTFEFI 

      1150       1160       1170       1180       1190       1200 
NKITEETDQD YYDVRRNLRL KVYSVLLDET SDKKLIRNIR EEFGTLLEGV LFFINSVELT 

      1210       1220       1230       1240       1250       1260 
FSCITSQENE EASDSETSLS DHTTEIKEIL FKVLGNVLQI LPVDEFVNAV LPLLSTSTNE 

      1270       1280       1290       1300       1310       1320 
DIRYHLTLVI GSKFELEGSE AIPIVNNVMK VLLDRMPLES KSVVISQVIL NTMTALVSKY 

      1330       1340       1350       1360       1370       1380 
GKKLEGSILT QALTLATEKV SSDMTEVKIS SLALITNCVQ VLGVKSIAFY PKIVPPSIKL 

      1390       1400       1410       1420       1430       1440 
FDASLADSSN PLKEQLQVAI LLLFAGLIKR IPSFLMSNIL DVLHVIYFSR EVDSSIRLSV 

      1450       1460       1470       1480       1490       1500 
ISLIIENIDL KEVLKVLFRI WSTEIATSND TVAVSLFLST LESTVENIDK KSATSQSPIF 

      1510       1520       1530       1540       1550       1560 
FKLLLSLFEF RSISSFDNNT ISRIEASVHE ISNSYVLKMN DKVFRPLFVI LVRWAFDGEG 

      1570       1580       1590       1600       1610       1620 
VTNAGITETE RLLAFFKFFN KLQENLRGII TSYFTYLLEP VDMLLKRFIS KDMENVNLRR 

      1630       1640       1650       1660       1670       1680 
LVINSLTSSL KFDRDEYWKS TSRFELISVS LVNQLSNIEN SIGKYLVKAI GALASNNSGV 

      1690       1700       1710       1720       1730       1740 
DEHNQILNKL IVEHMKASCS SNEKLWAIRA MKLIYSKIGE SWLVLLPQLV PVIAELLEDD 

      1750       1760 
DEEIEREVRT GLVKVVENVL GEPFDRYLD 

« Hide

References

« Hide 'large scale' references
[1]"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
Rasmussen S.W.
Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis."
Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J.
Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
[9]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes."
Wade C.H., Umbarger M.A., McAlear M.A.
Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation."
Dez C., Dlakic M., Tollervey D.
RNA 13:1516-1527(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SNORNA U3 AND PRE-RIBOSOMAL RNA, SUBCELLULAR LOCATION.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85021 Genomic DNA. Translation: CAA59385.1.
Z49384 Genomic DNA. Translation: CAA89404.1.
BK006943 Genomic DNA. Translation: DAA08691.1.
PIRS53378.
RefSeqNP_012426.1. NM_001181542.1.

3D structure databases

ProteinModelPortalP42945.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33647. 88 interactions.
DIPDIP-6466N.
IntActP42945. 57 interactions.
MINTMINT-671263.
STRING4932.YJL109C.

Proteomic databases

MaxQBP42945.
PaxDbP42945.
PeptideAtlasP42945.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL109C; YJL109C; YJL109C.
GeneID853335.
KEGGsce:YJL109C.

Organism-specific databases

CYGDYJL109c.
SGDS000003645. UTP10.

Phylogenomic databases

eggNOGNOG116525.
GeneTreeENSGT00390000015845.
HOGENOMHOG000093519.
KOK14550.
OMAKAFDLRR.
OrthoDBEOG7KM623.

Enzyme and pathway databases

BioCycYEAST:G3O-31563-MONOMER.

Gene expression databases

GenevestigatorP42945.

Family and domain databases

Gene3D1.25.10.10. 8 hits.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012954. BP28_C_dom.
IPR021133. HEAT_type_2.
IPR022125. U3snoRNP10.
[Graphical view]
PfamPF08146. BP28CT. 1 hit.
PF12397. U3snoRNP10. 1 hit.
[Graphical view]
SMARTSM01036. BP28CT. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 7 hits.
PROSITEPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973709.
PROP42945.

Entry information

Entry nameUTP10_YEAST
AccessionPrimary (citable) accession number: P42945
Secondary accession number(s): D6VW75
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families