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Protein

U3 small nucleolar RNA-associated protein 10

Gene

UTP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Involved in ribosome biosynthesis.4 Publications

GO - Molecular functioni

  1. snoRNA binding Source: SGD

GO - Biological processi

  1. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  3. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  5. positive regulation of transcription from RNA polymerase I promoter Source: SGD
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31563-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
U3 small nucleolar RNA-associated protein 10
Short name:
U3 snoRNA-associated protein 10
Alternative name(s):
U three protein 10
U3 protein 10 required for transcription
t-UTP10
Gene namesi
Name:UTP10
Ordered Locus Names:YJL109C
ORF Names:J0808
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL109c.
SGDiS000003645. UTP10.

Subcellular locationi

Nucleusnucleolus. Mitochondrion
Note: Associated with ribosomal chromatin, even in the absence of transcription.

GO - Cellular componenti

  1. 90S preribosome Source: SGD
  2. mitochondrion Source: UniProtKB-SubCell
  3. nucleolus Source: SGD
  4. preribosome, small subunit precursor Source: SGD
  5. rDNA heterochromatin Source: SGD
  6. small-subunit processome Source: SGD
  7. t-UTP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 17691768U3 small nucleolar RNA-associated protein 10PRO_0000186207Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP42945.
PaxDbiP42945.
PeptideAtlasiP42945.

Expressioni

Gene expression databases

GenevestigatoriP42945.

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
UTP4Q066794EBI-1884,EBI-35712

Protein-protein interaction databases

BioGridi33647. 89 interactions.
DIPiDIP-6466N.
IntActiP42945. 57 interactions.
MINTiMINT-671263.
STRINGi4932.YJL109C.

Structurei

3D structure databases

ProteinModelPortaliP42945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1729 – 176739HEATAdd
BLAST

Sequence similaritiesi

Belongs to the HEATR1/UTP10 family.Curated
Contains 1 HEAT repeat.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG116525.
GeneTreeiENSGT00390000015845.
HOGENOMiHOG000093519.
InParanoidiP42945.
KOiK14550.
OMAiKAFDLRR.
OrthoDBiEOG7KM623.

Family and domain databases

Gene3Di1.25.10.10. 8 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012954. BP28_C_dom.
IPR021133. HEAT_type_2.
IPR022125. U3snoRNP10.
[Graphical view]
PfamiPF08146. BP28CT. 1 hit.
PF12397. U3snoRNP10. 1 hit.
[Graphical view]
SMARTiSM01036. BP28CT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42945-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE
60 70 80 90 100
NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKEE IKDLDNAINA
110 120 130 140 150
YLLLASSKWY LAPTLHATEW LVRRFQIHVK NTEMLLLSTL NYYQTPVFKR
160 170 180 190 200
ILSIIKLPPL FNCLSNFVRS EKPPTALTMI KLFNDMDFLK LYTSYLDQCI
210 220 230 240 250
KHNATYTNQL LFTTCCFINV VAFNSNNDEK LNQLVPILLE ISAKLLASKS
260 270 280 290 300
KDCQIAAHTI LVVFATALPL KKTIILAAME TILSNLDAKE AKHSALLTIC
310 320 330 340 350
KLFQTLKGQG NVDQLPSKIF KLFDSKFDTV SILTFLDKED KPVCDKFITS
360 370 380 390 400
YTRSIARYDR SKLNIILSLL KKIRLERYEV RLIITDLIYL SEILEDKSQL
410 420 430 440 450
VELFEYFISI NEDLVLKCLK SLGLTGELFE IRLTTSLFTN ADVNTDIVKQ
460 470 480 490 500
LSDPVETTKK DTASFQTFLD KHSELINTTN VSMLTETGER YKKVLSLFTE
510 520 530 540 550
AIGKGYKASS FLTSFFTTLE SRITFLLRVT ISPAAPTALK LISLNNIAKY
560 570 580 590 600
INSIEKEVNI FTLVPCLICA LRDASIKVRT GVKKILSLIA KRPSTKHYFL
610 620 630 640 650
SDKLYGENVT IPMLNPKDSE AWLSGFLNEY VTENYDISRI LTPKRNEKVF
660 670 680 690 700
LMFWANQALL IPSPYAKTVL LDNLNKSPTY ASSYSSLFEE FISHYLENRS
710 720 730 740 750
SWEKSCIANK TNFEHFERSL VNLVSPKEKQ SFMIDFVLSA LNSDYEQLAN
760 770 780 790 800
IAAERLISIF ASLNNAQKLK IVQNIVDSSS NVESSYDTVG VLQSLPLDSD
810 820 830 840 850
IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV SQLAELHLRK
860 870 880 890 900
LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGLP VLYAQETLIS
910 920 930 940 950
CTLNTITYLK EHGCTELTNV RADILVSAIR NSASPQVQNK LLLVIGSLAT
960 970 980 990 1000
LSSEVILHSV MPIFTFMGAH SIRQDDEFTT KVVERTILTV VPALIKNSKG
1010 1020 1030 1040 1050
NEKEEMEFLL LSFTTALQHV PRHRRVKLFS TLIKTLDPVK ALGSFLFLIA
1060 1070 1080 1090 1100
QQYSSALVNF KIGEARILIE FIKALLVDLH VNEELSGLND LLDIIKLLTS
1110 1120 1130 1140 1150
SKSSSEKKKS LESRVLFSNG VLNFSESEFL TFMNNTFEFI NKITEETDQD
1160 1170 1180 1190 1200
YYDVRRNLRL KVYSVLLDET SDKKLIRNIR EEFGTLLEGV LFFINSVELT
1210 1220 1230 1240 1250
FSCITSQENE EASDSETSLS DHTTEIKEIL FKVLGNVLQI LPVDEFVNAV
1260 1270 1280 1290 1300
LPLLSTSTNE DIRYHLTLVI GSKFELEGSE AIPIVNNVMK VLLDRMPLES
1310 1320 1330 1340 1350
KSVVISQVIL NTMTALVSKY GKKLEGSILT QALTLATEKV SSDMTEVKIS
1360 1370 1380 1390 1400
SLALITNCVQ VLGVKSIAFY PKIVPPSIKL FDASLADSSN PLKEQLQVAI
1410 1420 1430 1440 1450
LLLFAGLIKR IPSFLMSNIL DVLHVIYFSR EVDSSIRLSV ISLIIENIDL
1460 1470 1480 1490 1500
KEVLKVLFRI WSTEIATSND TVAVSLFLST LESTVENIDK KSATSQSPIF
1510 1520 1530 1540 1550
FKLLLSLFEF RSISSFDNNT ISRIEASVHE ISNSYVLKMN DKVFRPLFVI
1560 1570 1580 1590 1600
LVRWAFDGEG VTNAGITETE RLLAFFKFFN KLQENLRGII TSYFTYLLEP
1610 1620 1630 1640 1650
VDMLLKRFIS KDMENVNLRR LVINSLTSSL KFDRDEYWKS TSRFELISVS
1660 1670 1680 1690 1700
LVNQLSNIEN SIGKYLVKAI GALASNNSGV DEHNQILNKL IVEHMKASCS
1710 1720 1730 1740 1750
SNEKLWAIRA MKLIYSKIGE SWLVLLPQLV PVIAELLEDD DEEIEREVRT
1760
GLVKVVENVL GEPFDRYLD
Length:1,769
Mass (Da):200,082
Last modified:November 1, 1995 - v1
Checksum:i064480D1D249B241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85021 Genomic DNA. Translation: CAA59385.1.
Z49384 Genomic DNA. Translation: CAA89404.1.
BK006943 Genomic DNA. Translation: DAA08691.1.
PIRiS53378.
RefSeqiNP_012426.1. NM_001181542.1.

Genome annotation databases

EnsemblFungiiYJL109C; YJL109C; YJL109C.
GeneIDi853335.
KEGGisce:YJL109C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85021 Genomic DNA. Translation: CAA59385.1.
Z49384 Genomic DNA. Translation: CAA89404.1.
BK006943 Genomic DNA. Translation: DAA08691.1.
PIRiS53378.
RefSeqiNP_012426.1. NM_001181542.1.

3D structure databases

ProteinModelPortaliP42945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33647. 89 interactions.
DIPiDIP-6466N.
IntActiP42945. 57 interactions.
MINTiMINT-671263.
STRINGi4932.YJL109C.

Proteomic databases

MaxQBiP42945.
PaxDbiP42945.
PeptideAtlasiP42945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL109C; YJL109C; YJL109C.
GeneIDi853335.
KEGGisce:YJL109C.

Organism-specific databases

CYGDiYJL109c.
SGDiS000003645. UTP10.

Phylogenomic databases

eggNOGiNOG116525.
GeneTreeiENSGT00390000015845.
HOGENOMiHOG000093519.
InParanoidiP42945.
KOiK14550.
OMAiKAFDLRR.
OrthoDBiEOG7KM623.

Enzyme and pathway databases

BioCyciYEAST:G3O-31563-MONOMER.

Miscellaneous databases

NextBioi973709.
PROiP42945.

Gene expression databases

GenevestigatoriP42945.

Family and domain databases

Gene3Di1.25.10.10. 8 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012954. BP28_C_dom.
IPR021133. HEAT_type_2.
IPR022125. U3snoRNP10.
[Graphical view]
PfamiPF08146. BP28CT. 1 hit.
PF12397. U3snoRNP10. 1 hit.
[Graphical view]
SMARTiSM01036. BP28CT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
    Rasmussen S.W.
    Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
    Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
    Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
  9. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes."
    Wade C.H., Umbarger M.A., McAlear M.A.
    Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation."
    Dez C., Dlakic M., Tollervey D.
    RNA 13:1516-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNORNA U3 AND PRE-RIBOSOMAL RNA, SUBCELLULAR LOCATION.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUTP10_YEAST
AccessioniPrimary (citable) accession number: P42945
Secondary accession number(s): D6VW75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.