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P42945

- UTP10_YEAST

UniProt

P42945 - UTP10_YEAST

Protein

U3 small nucleolar RNA-associated protein 10

Gene

UTP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Involved in ribosome biosynthesis.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. snoRNA binding Source: SGD

    GO - Biological processi

    1. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    5. positive regulation of transcription from RNA polymerase I promoter Source: SGD
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing, Transcription

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31563-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U3 small nucleolar RNA-associated protein 10
    Short name:
    U3 snoRNA-associated protein 10
    Alternative name(s):
    U three protein 10
    U3 protein 10 required for transcription
    t-UTP10
    Gene namesi
    Name:UTP10
    Ordered Locus Names:YJL109C
    ORF Names:J0808
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL109c.
    SGDiS000003645. UTP10.

    Subcellular locationi

    Nucleusnucleolus. Mitochondrion
    Note: Associated with ribosomal chromatin, even in the absence of transcription.

    GO - Cellular componenti

    1. 90S preribosome Source: SGD
    2. mitochondrion Source: UniProtKB-SubCell
    3. nucleolus Source: SGD
    4. preribosome, small subunit precursor Source: SGD
    5. rDNA heterochromatin Source: SGD
    6. small-subunit processome Source: SGD
    7. t-UTP complex Source: SGD

    Keywords - Cellular componenti

    Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 17691768U3 small nucleolar RNA-associated protein 10PRO_0000186207Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP42945.
    PaxDbiP42945.
    PeptideAtlasiP42945.

    Expressioni

    Gene expression databases

    GenevestigatoriP42945.

    Interactioni

    Subunit structurei

    Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    UTP4Q066794EBI-1884,EBI-35712

    Protein-protein interaction databases

    BioGridi33647. 88 interactions.
    DIPiDIP-6466N.
    IntActiP42945. 57 interactions.
    MINTiMINT-671263.
    STRINGi4932.YJL109C.

    Structurei

    3D structure databases

    ProteinModelPortaliP42945.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1729 – 176739HEATAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HEATR1/UTP10 family.Curated
    Contains 1 HEAT repeat.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG116525.
    GeneTreeiENSGT00390000015845.
    HOGENOMiHOG000093519.
    KOiK14550.
    OMAiKAFDLRR.
    OrthoDBiEOG7KM623.

    Family and domain databases

    Gene3Di1.25.10.10. 8 hits.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR012954. BP28_C_dom.
    IPR021133. HEAT_type_2.
    IPR022125. U3snoRNP10.
    [Graphical view]
    PfamiPF08146. BP28CT. 1 hit.
    PF12397. U3snoRNP10. 1 hit.
    [Graphical view]
    SMARTiSM01036. BP28CT. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 7 hits.
    PROSITEiPS50077. HEAT_REPEAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42945-1 [UniParc]FASTAAdd to Basket

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    MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE     50
    NASKALEELS QIEPKFAIFS RTLFSESSIS LDRNVQTKEE IKDLDNAINA 100
    YLLLASSKWY LAPTLHATEW LVRRFQIHVK NTEMLLLSTL NYYQTPVFKR 150
    ILSIIKLPPL FNCLSNFVRS EKPPTALTMI KLFNDMDFLK LYTSYLDQCI 200
    KHNATYTNQL LFTTCCFINV VAFNSNNDEK LNQLVPILLE ISAKLLASKS 250
    KDCQIAAHTI LVVFATALPL KKTIILAAME TILSNLDAKE AKHSALLTIC 300
    KLFQTLKGQG NVDQLPSKIF KLFDSKFDTV SILTFLDKED KPVCDKFITS 350
    YTRSIARYDR SKLNIILSLL KKIRLERYEV RLIITDLIYL SEILEDKSQL 400
    VELFEYFISI NEDLVLKCLK SLGLTGELFE IRLTTSLFTN ADVNTDIVKQ 450
    LSDPVETTKK DTASFQTFLD KHSELINTTN VSMLTETGER YKKVLSLFTE 500
    AIGKGYKASS FLTSFFTTLE SRITFLLRVT ISPAAPTALK LISLNNIAKY 550
    INSIEKEVNI FTLVPCLICA LRDASIKVRT GVKKILSLIA KRPSTKHYFL 600
    SDKLYGENVT IPMLNPKDSE AWLSGFLNEY VTENYDISRI LTPKRNEKVF 650
    LMFWANQALL IPSPYAKTVL LDNLNKSPTY ASSYSSLFEE FISHYLENRS 700
    SWEKSCIANK TNFEHFERSL VNLVSPKEKQ SFMIDFVLSA LNSDYEQLAN 750
    IAAERLISIF ASLNNAQKLK IVQNIVDSSS NVESSYDTVG VLQSLPLDSD 800
    IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV SQLAELHLRK 850
    LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGLP VLYAQETLIS 900
    CTLNTITYLK EHGCTELTNV RADILVSAIR NSASPQVQNK LLLVIGSLAT 950
    LSSEVILHSV MPIFTFMGAH SIRQDDEFTT KVVERTILTV VPALIKNSKG 1000
    NEKEEMEFLL LSFTTALQHV PRHRRVKLFS TLIKTLDPVK ALGSFLFLIA 1050
    QQYSSALVNF KIGEARILIE FIKALLVDLH VNEELSGLND LLDIIKLLTS 1100
    SKSSSEKKKS LESRVLFSNG VLNFSESEFL TFMNNTFEFI NKITEETDQD 1150
    YYDVRRNLRL KVYSVLLDET SDKKLIRNIR EEFGTLLEGV LFFINSVELT 1200
    FSCITSQENE EASDSETSLS DHTTEIKEIL FKVLGNVLQI LPVDEFVNAV 1250
    LPLLSTSTNE DIRYHLTLVI GSKFELEGSE AIPIVNNVMK VLLDRMPLES 1300
    KSVVISQVIL NTMTALVSKY GKKLEGSILT QALTLATEKV SSDMTEVKIS 1350
    SLALITNCVQ VLGVKSIAFY PKIVPPSIKL FDASLADSSN PLKEQLQVAI 1400
    LLLFAGLIKR IPSFLMSNIL DVLHVIYFSR EVDSSIRLSV ISLIIENIDL 1450
    KEVLKVLFRI WSTEIATSND TVAVSLFLST LESTVENIDK KSATSQSPIF 1500
    FKLLLSLFEF RSISSFDNNT ISRIEASVHE ISNSYVLKMN DKVFRPLFVI 1550
    LVRWAFDGEG VTNAGITETE RLLAFFKFFN KLQENLRGII TSYFTYLLEP 1600
    VDMLLKRFIS KDMENVNLRR LVINSLTSSL KFDRDEYWKS TSRFELISVS 1650
    LVNQLSNIEN SIGKYLVKAI GALASNNSGV DEHNQILNKL IVEHMKASCS 1700
    SNEKLWAIRA MKLIYSKIGE SWLVLLPQLV PVIAELLEDD DEEIEREVRT 1750
    GLVKVVENVL GEPFDRYLD 1769
    Length:1,769
    Mass (Da):200,082
    Last modified:November 1, 1995 - v1
    Checksum:i064480D1D249B241
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85021 Genomic DNA. Translation: CAA59385.1.
    Z49384 Genomic DNA. Translation: CAA89404.1.
    BK006943 Genomic DNA. Translation: DAA08691.1.
    PIRiS53378.
    RefSeqiNP_012426.1. NM_001181542.1.

    Genome annotation databases

    EnsemblFungiiYJL109C; YJL109C; YJL109C.
    GeneIDi853335.
    KEGGisce:YJL109C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85021 Genomic DNA. Translation: CAA59385.1 .
    Z49384 Genomic DNA. Translation: CAA89404.1 .
    BK006943 Genomic DNA. Translation: DAA08691.1 .
    PIRi S53378.
    RefSeqi NP_012426.1. NM_001181542.1.

    3D structure databases

    ProteinModelPortali P42945.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33647. 88 interactions.
    DIPi DIP-6466N.
    IntActi P42945. 57 interactions.
    MINTi MINT-671263.
    STRINGi 4932.YJL109C.

    Proteomic databases

    MaxQBi P42945.
    PaxDbi P42945.
    PeptideAtlasi P42945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL109C ; YJL109C ; YJL109C .
    GeneIDi 853335.
    KEGGi sce:YJL109C.

    Organism-specific databases

    CYGDi YJL109c.
    SGDi S000003645. UTP10.

    Phylogenomic databases

    eggNOGi NOG116525.
    GeneTreei ENSGT00390000015845.
    HOGENOMi HOG000093519.
    KOi K14550.
    OMAi KAFDLRR.
    OrthoDBi EOG7KM623.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31563-MONOMER.

    Miscellaneous databases

    NextBioi 973709.
    PROi P42945.

    Gene expression databases

    Genevestigatori P42945.

    Family and domain databases

    Gene3Di 1.25.10.10. 8 hits.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR012954. BP28_C_dom.
    IPR021133. HEAT_type_2.
    IPR022125. U3snoRNP10.
    [Graphical view ]
    Pfami PF08146. BP28CT. 1 hit.
    PF12397. U3snoRNP10. 1 hit.
    [Graphical view ]
    SMARTi SM01036. BP28CT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 7 hits.
    PROSITEi PS50077. HEAT_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
      Rasmussen S.W.
      Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components."
      Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J.
      Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
    9. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
      Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
      J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. "The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes."
      Wade C.H., Umbarger M.A., McAlear M.A.
      Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation."
      Dez C., Dlakic M., Tollervey D.
      RNA 13:1516-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SNORNA U3 AND PRE-RIBOSOMAL RNA, SUBCELLULAR LOCATION.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUTP10_YEAST
    AccessioniPrimary (citable) accession number: P42945
    Secondary accession number(s): D6VW75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 18200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3