P42945 (UTP10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 10
Short name=U3 snoRNA-associated protein 10

Alternative name(s):
U three protein 10
U3 protein 10 required for transcription
t-UTP10

Gene names

Name:	UTP10
Ordered Locus Names:	YJL109C
ORF Names:	J0808

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1769 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I together with a subset of U3 proteins required for transcription (t-UTPs). Involved in ribosome biosynthesis. Ref.4 Ref.8 Ref.10 Ref.11
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. In the absence of snoRNA3, forms a complex with other t-UTPs. This complex can associate with pre-18S ribosomal RNAs. Ref.4 Ref.8 Ref.11
Subcellular location	Nucleus › nucleolus. Mitochondrion. Note: Associated with ribosomal chromatin, even in the absence of transcription. Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.11
Miscellaneous	Present with 18200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the HEATR1/UTP10 family. Contains 1 HEAT repeat.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transcription rRNA processing
Cellular component	Mitochondrion Nucleus
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.11. Source: SGD positive regulation of transcription from RNA polymerase I promoter Inferred from mutant phenotype Ref.8. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay Ref.7Ref.9. Source: SGD preribosome, small subunit precursor Inferred from direct assay Ref.11. Source: SGD rDNA heterochromatin Inferred from direct assay Ref.8. Source: SGD small-subunit processome Inferred from direct assay Ref.4. Source: SGD t-UTP complex Inferred from direct assay PubMed 17515605. Source: SGD
Molecular_function	snoRNA binding Inferred from physical interaction Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UTP4	Q06679	4	EBI-1884,EBI-35712

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1769	1769	U3 small nucleolar RNA-associated protein 10		PRO_0000186207
Regions
	Repeat	1729 – 1767	39	HEAT
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P42945 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 064480D1D249B241	FASTA	1,769	200,082
	10 20 30 40 50 60 MSSLSDQLAQ VASNNATVAL DRKRRQKLHS ASLIYNSKTA ATQDYDFIFE NASKALEELS 70 80 90 100 110 120 QIEPKFAIFS RTLFSESSIS LDRNVQTKEE IKDLDNAINA YLLLASSKWY LAPTLHATEW 130 140 150 160 170 180 LVRRFQIHVK NTEMLLLSTL NYYQTPVFKR ILSIIKLPPL FNCLSNFVRS EKPPTALTMI 190 200 210 220 230 240 KLFNDMDFLK LYTSYLDQCI KHNATYTNQL LFTTCCFINV VAFNSNNDEK LNQLVPILLE 250 260 270 280 290 300 ISAKLLASKS KDCQIAAHTI LVVFATALPL KKTIILAAME TILSNLDAKE AKHSALLTIC 310 320 330 340 350 360 KLFQTLKGQG NVDQLPSKIF KLFDSKFDTV SILTFLDKED KPVCDKFITS YTRSIARYDR 370 380 390 400 410 420 SKLNIILSLL KKIRLERYEV RLIITDLIYL SEILEDKSQL VELFEYFISI NEDLVLKCLK 430 440 450 460 470 480 SLGLTGELFE IRLTTSLFTN ADVNTDIVKQ LSDPVETTKK DTASFQTFLD KHSELINTTN 490 500 510 520 530 540 VSMLTETGER YKKVLSLFTE AIGKGYKASS FLTSFFTTLE SRITFLLRVT ISPAAPTALK 550 560 570 580 590 600 LISLNNIAKY INSIEKEVNI FTLVPCLICA LRDASIKVRT GVKKILSLIA KRPSTKHYFL 610 620 630 640 650 660 SDKLYGENVT IPMLNPKDSE AWLSGFLNEY VTENYDISRI LTPKRNEKVF LMFWANQALL 670 680 690 700 710 720 IPSPYAKTVL LDNLNKSPTY ASSYSSLFEE FISHYLENRS SWEKSCIANK TNFEHFERSL 730 740 750 760 770 780 VNLVSPKEKQ SFMIDFVLSA LNSDYEQLAN IAAERLISIF ASLNNAQKLK IVQNIVDSSS 790 800 810 820 830 840 NVESSYDTVG VLQSLPLDSD IFVSILNQNS ISNEMDQTDF SKRRRRRSST SKNAFLKEEV 850 860 870 880 890 900 SQLAELHLRK LTIILEALDK VRNVGSEKLL FTLLSLLSDL ETLDQDGGLP VLYAQETLIS 910 920 930 940 950 960 CTLNTITYLK EHGCTELTNV RADILVSAIR NSASPQVQNK LLLVIGSLAT LSSEVILHSV 970 980 990 1000 1010 1020 MPIFTFMGAH SIRQDDEFTT KVVERTILTV VPALIKNSKG NEKEEMEFLL LSFTTALQHV 1030 1040 1050 1060 1070 1080 PRHRRVKLFS TLIKTLDPVK ALGSFLFLIA QQYSSALVNF KIGEARILIE FIKALLVDLH 1090 1100 1110 1120 1130 1140 VNEELSGLND LLDIIKLLTS SKSSSEKKKS LESRVLFSNG VLNFSESEFL TFMNNTFEFI 1150 1160 1170 1180 1190 1200 NKITEETDQD YYDVRRNLRL KVYSVLLDET SDKKLIRNIR EEFGTLLEGV LFFINSVELT 1210 1220 1230 1240 1250 1260 FSCITSQENE EASDSETSLS DHTTEIKEIL FKVLGNVLQI LPVDEFVNAV LPLLSTSTNE 1270 1280 1290 1300 1310 1320 DIRYHLTLVI GSKFELEGSE AIPIVNNVMK VLLDRMPLES KSVVISQVIL NTMTALVSKY 1330 1340 1350 1360 1370 1380 GKKLEGSILT QALTLATEKV SSDMTEVKIS SLALITNCVQ VLGVKSIAFY PKIVPPSIKL 1390 1400 1410 1420 1430 1440 FDASLADSSN PLKEQLQVAI LLLFAGLIKR IPSFLMSNIL DVLHVIYFSR EVDSSIRLSV 1450 1460 1470 1480 1490 1500 ISLIIENIDL KEVLKVLFRI WSTEIATSND TVAVSLFLST LESTVENIDK KSATSQSPIF 1510 1520 1530 1540 1550 1560 FKLLLSLFEF RSISSFDNNT ISRIEASVHE ISNSYVLKMN DKVFRPLFVI LVRWAFDGEG 1570 1580 1590 1600 1610 1620 VTNAGITETE RLLAFFKFFN KLQENLRGII TSYFTYLLEP VDMLLKRFIS KDMENVNLRR 1630 1640 1650 1660 1670 1680 LVINSLTSSL KFDRDEYWKS TSRFELISVS LVNQLSNIEN SIGKYLVKAI GALASNNSGV 1690 1700 1710 1720 1730 1740 DEHNQILNKL IVEHMKASCS SNEKLWAIRA MKLIYSKIGE SWLVLLPQLV PVIAELLEDD 1750 1760 DEEIEREVRT GLVKVVENVL GEPFDRYLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)." Rasmussen S.W. Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[8]	"RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components." Gallagher J.E.G., Dunbar D.A., Granneman S., Mitchell B.M., Osheim Y., Beyer A.L., Baserga S.J. Genes Dev. 18:2506-2517(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN COMPLEX WITH OTHER T-UTPS, SUBCELLULAR LOCATION.
[9]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes." Wade C.H., Umbarger M.A., McAlear M.A. Yeast 23:293-306(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Roles of the HEAT repeat proteins Utp10 and Utp20 in 40S ribosome maturation." Dez C., Dlakic M., Tollervey D. RNA 13:1516-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNORNA U3 AND PRE-RIBOSOMAL RNA, SUBCELLULAR LOCATION.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85021 Genomic DNA. Translation: CAA59385.1. Z49384 Genomic DNA. Translation: CAA89404.1. BK006943 Genomic DNA. Translation: DAA08691.1.
PIR	S53378.
RefSeq	NP_012426.1. NM_001181542.1.
3D structure databases
ProteinModelPortal	P42945.
SMR	P42945. Positions 562-589, 1283-1323, 1666-1768.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6466N.
IntAct	P42945. 57 interactions.
MINT	MINT-671263.
STRING	4932.YJL109C.
Proteomic databases
PaxDb	P42945.
PeptideAtlas	P42945.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL109C; YJL109C; YJL109C.
GeneID	853335.
KEGG	sce:YJL109C.
Organism-specific databases
CYGD	YJL109c.
SGD	S000003645. UTP10.
Phylogenomic databases
eggNOG	NOG116525.
GeneTree	ENSGT00390000015845.
HOGENOM	HOG000093519.
KO	K14550.
OMA	VLHSVMP.
OrthoDB	EOG4C5GSH.
Gene expression databases
Genevestigator	P42945.
GermOnline	YJL109C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 8 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR012954. BP28_C_dom. IPR021133. HEAT_type_2. IPR022125. U3snoRNP10. [Graphical view]
Pfam	PF08146. BP28CT. 1 hit. PF12397. U3snoRNP10. 1 hit. [Graphical view]
SMART	SM01036. BP28CT. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973709.

Entry information

Entry name

UTP10_YEAST

Accession

Primary (citable) accession number: P42945
Secondary accession number(s): D6VW75

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program