Reviewed, UniProtKB/Swiss-Prot P42944 (GZF3_YEAST)

Names and origin

Protein names

Recommended name:
    Protein GZF3

Gene names

Name:	GZF3
Ordered Locus Names:	YJL110C
ORF Names:	J0806

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Nucleus Potential.
Miscellaneous	Present with 319 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 GATA-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD regulation of nitrogen utilization Inferred from genetic interaction. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD specific RNA polymerase II transcription factor activity Inferred from mutant phenotype. Source: SGD transcription factor activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HDA1	P53973	2	EBI-8042,EBI-8206
IRA2	P19158	1	EBI-8042,EBI-9354

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Protein GZF3		PRO_0000083488
Regions
	Zinc finger	131 – 155	25	GATA-type
Amino acid modifications
	Modified residue	403	1	Phosphoserine Ref.5
	Modified residue	404	1	Phosphoserine Ref.5
	Modified residue	443	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P42944-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 807243799BFAB704	FASTA	551	60,520
	10 20 30 40 50 60 MASQATTLRG YNIRKRDNVF EPKSSENLNS LNQSEEEGHI GRWPPLGYEA VSAEQKSAVQ 70 80 90 100 110 120 LRESQAGASI SNNMNFKAND KSFSTSTAGR MSPDTNSLHH ILPKNQVKNN GQTMDANCNN 130 140 150 160 170 180 NVSNDANVPV CKNCLTSTTP LWRRDEHGAM LCNACGLFLK LHGKPRPISL KTDVIKSRNR 190 200 210 220 230 240 KSNTNHAHNL DNFRNQTLIA ELKGDCNIES SGRKANRVTS EDKKKKSSQL LMGTSSTAKI 250 260 270 280 290 300 SKKPKTESKE RSDSHLSATK LEVLMSGDCS RPNLKPKLPK QDTAIYQEKL LTFPSYTDVK 310 320 330 340 350 360 EYSNSAHQSA FIKERSQFNA ASFPLNASHS VTSKTGADSP QLPHLSMLLG SLSSTSISNN 370 380 390 400 410 420 GSEIVSNCNN GIASTAATLA PTSSRTTDSN PSEVPNQIRS TMSSPDIISA KRNDPAPLSF 430 440 450 460 470 480 HMASINDMLE TRDRAISNVK TETTPPHFIP FLQSSKAPCI SKANSQSISN SVSSSDVSGR 490 500 510 520 530 540 KFENHPAKDL GDQLSTKLHK EEEIIKLKTR INELELVTDL YRRHINELDG KCRALEERLQ 550 RTVKQEGNKG G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)." Rasmussen S.W. Yeast 11:873-883(1995) [PubMed: 7483851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon." Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C. Yeast 12:1471-1474(1996) [PubMed: 8948101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-403; SER-404 AND THR-443, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85021 Genomic DNA. Translation: CAA59384.1. Z49385 Genomic DNA. Translation: CAA89405.1. X86353 Genomic DNA. Translation: CAA60126.1.
PIR	S53377.
RefSeq	NP_012425.1.
3D structure databases
SMR	P42944. Positions 131-182.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1359N.
IntAct	P42944. 11 interactions.
STRING	P42944.
Genome annotation databases
Ensembl	YJL110C; YJL110C; YJL110C; Saccharomyces cerevisiae. [Genome view]
GeneID	853334.
KEGG	sce:YJL110C.
NMPDR	fig|4932.3.peg.3394.
Organism-specific databases
CYGD	YJL110c.
SGD	S000003646. GZF3.
Phylogenomic databases
OrthoDB	EOG9J12R9.
Gene expression databases
ArrayExpress	P42944.
Genevestigator	P42944.
GermOnline	YJL110C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000679. Znf_GATA. IPR013088. Znf_NHR/GATA. [Graphical view]
Gene3D	G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.
Pfam	PF00320. GATA. 1 hit. [Graphical view]
PRINTS	PR00619. GATAZNFINGER.
SMART	SM00401. ZnF_GATA. 1 hit. [Graphical view]
PROSITE	PS00344. GATA_ZN_FINGER_1. 1 hit. PS50114. GATA_ZN_FINGER_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973706.

Entry information

Entry name

GZF3_YEAST

Accession

Primary (citable) accession number: P42944

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)