P42941 (SERB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine phosphatase
Short name=PSP
Short name=PSPase
EC=3.1.3.3

Alternative name(s):
O-phosphoserine phosphohydrolase

Gene names

Name:	SER2
Ordered Locus Names:	YGR208W
ORF Names:	G7744

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
Miscellaneous	Present with 13800 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the serB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	magnesium ion binding Inferred from Biological aspect of Ancestor. Source: RefGenome phosphoserine phosphatase activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Phosphoserine phosphatase		PRO_0000156885
Regions
	Region	186 – 187	2	Substrate binding By similarity
Sites
	Active site	97	1	Nucleophile By similarity
	Active site	99	1	Proton donor By similarity
	Metal binding	97	1	Magnesium By similarity
	Metal binding	99	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	255	1	Magnesium By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42941 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 11CD560B3A96172B	FASTA	309	34,207
	10 20 30 40 50 60 MSKFVITCIA HGENLPKETI DQIAKEITES SAKDVSINGT KKLSARATDI FIEVAGSIVQ 70 80 90 100 110 120 KDLKNKLTNV IDSHNDVDVI VSVDNEYRQA KKLFVFDMDS TLIYQEVIEL IAAYAGVEEQ 130 140 150 160 170 180 VHEITERAMN NELDFKESLR ERVKLLQGLQ VDTLYDEIKQ KLEVTKGVPE LCKFLHKKNC 190 200 210 220 230 240 KLAVLSGGFI QFAGFIKDQL GLDFCKANLL EVDTDGKLTG KTLGPIVDGQ CKSETLLQLC 250 260 270 280 290 300 NDYNVPVEAS CMVGDGGNDL PAMATAGFGI AWNAKPKVQK AAPCKLNTKS MTDILYILGY TDDEIYNRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Song J.M., Cheung E., Rabinowitz J.C. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs." Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C. Yeast 12:273-280(1996) [PubMed: 8904340] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36473 Genomic DNA. Translation: AAA79062.1. Z49133 Genomic DNA. Translation: CAA89001.1. Z72993 Genomic DNA. Translation: CAA97235.1. BK006941 Genomic DNA. Translation: DAA08302.1.
PIR	S53931.
RefSeq	NP_011724.1. NM_001181337.1.
3D structure databases
ProteinModelPortal	P42941.
SMR	P42941. Positions 2-306.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2085N.
IntAct	P42941. 10 interactions.
MINT	MINT-545797.
STRING	P42941.
Proteomic databases
PeptideAtlas	P42941.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR208W; YGR208W; YGR208W.
GeneID	853122.
KEGG	sce:YGR208W.
NMPDR	fig|4932.3.peg.2852.
Organism-specific databases
CYGD	YGR208w.
SGD	S000003440. SER2.
Phylogenomic databases
eggNOG	fuNOG08484.
GeneTree	EFGT00050000003887.
HOGENOM	HBG645339.
OMA	ARIDHGD.
OrthoDB	EOG46X2HS.
Enzyme and pathway databases
BRENDA	3.1.3.3. 984.
Gene expression databases
ArrayExpress	P42941.
Genevestigator	P42941.
GermOnline	YGR208W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR023190. Pser_Pase_dom_2. IPR004469. SerB. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits. G3DSA:1.10.150.210. Pser_Pase_dom_2. 1 hit.
KO	K01079.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01488. HAD-SF-IB. 1 hit. TIGR00338. SerB. 1 hit.
ProtoNet	Search...
Other
NextBio	973160.

Entry information

Entry name

SERB_YEAST

Accession

Primary (citable) accession number: P42941
Secondary accession number(s): D6VUZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program