Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Multivesicular body sorting factor 12

Gene

MVB12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Appears to be involved in cargo sorting and release of the ESCRT-I complex from the MVBs.1 Publication

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • endosome transport via multivesicular body sorting pathway Source: SGD
  • negative regulation of protein complex assembly Source: SGD
  • protein targeting to vacuole Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30889-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Multivesicular body sorting factor 12
Alternative name(s):
12 kDa multivesicular body sorting factor
ESCRT-I complex subunit MVB12
Gene namesi
Name:MVB12
Ordered Locus Names:YGR206W
ORF Names:G7740
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR206W
SGDiS000003438 MVB12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47L → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57. 1
Mutagenesisi54C → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57. 1 Publication1
Mutagenesisi57I → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47. 1 Publication1
Mutagenesisi64H → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002028451 – 101Multivesicular body sorting factor 12Add BLAST101

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei94PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP42939
PaxDbiP42939
PRIDEiP42939

PTM databases

iPTMnetiP42939

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Interacts with STP22 and SRN2.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33459, 75 interactors
DIPiDIP-5332N
IntActiP42939, 10 interactors
MINTiP42939
STRINGi4932.YGR206W

Structurei

Secondary structure

1101
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Beta strandi15 – 19Combined sources5
Helixi41 – 65Combined sources25
Helixi67 – 78Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P22X-ray2.70D4-81[»]
ProteinModelPortaliP42939
SMRiP42939
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42939

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000939
InParanoidiP42939
KOiK12187
OMAiYEECDNI
OrthoDBiEOG092C5KPY

Family and domain databases

InterProiView protein in InterPro
IPR019014 Mvb12
PfamiView protein in Pfam
PF09452 Mvb12, 1 hit

Sequencei

Sequence statusi: Complete.

P42939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNVEELLR RIPLYNKYGK DFPQETVTRF QMPEFKLPAL QPTRDLLCPW
60 70 80 90 100
YEECDNITKV CQLHDSSNKK FDQWYKEQYL SKKPPGIVGN TLLSPSRKDN

S
Length:101
Mass (Da):11,968
Last modified:November 1, 1995 - v1
Checksum:iE48B085E0FACBAC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA Translation: CAA88999.1
Z72991 Genomic DNA Translation: CAA97233.1
AY692570 Genomic DNA Translation: AAT92589.1
BK006941 Genomic DNA Translation: DAA08300.1
PIRiS53929
RefSeqiNP_011722.3, NM_001181335.3

Genome annotation databases

EnsemblFungiiYGR206W; YGR206W; YGR206W
GeneIDi853120
KEGGisce:YGR206W

Similar proteinsi

Entry informationi

Entry nameiMVB12_YEAST
AccessioniPrimary (citable) accession number: P42939
Secondary accession number(s): D6VUY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health