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Protein

Multivesicular body sorting factor 12

Gene

MVB12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Appears to be involved in cargo sorting and release of the ESCRT-I complex from the MVBs.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • endosome transport via multivesicular body sorting pathway Source: SGD
  • negative regulation of protein complex assembly Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30889-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multivesicular body sorting factor 12
Alternative name(s):
12 kDa multivesicular body sorting factor
ESCRT-I complex subunit MVB12
Gene namesi
Name:MVB12
Ordered Locus Names:YGR206W
ORF Names:G7740
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR206W.
SGDiS000003438. MVB12.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Endosome 1 Publication
  • Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • cytosol Source: SGD
  • endosome Source: SGD
  • ESCRT I complex Source: SGD
  • late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47L → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57. 1
Mutagenesisi54C → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57. 1 Publication1
Mutagenesisi57I → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47. 1 Publication1
Mutagenesisi64H → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002028451 – 101Multivesicular body sorting factor 12Add BLAST101

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei94PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP42939.
PRIDEiP42939.

PTM databases

iPTMnetiP42939.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with STP22 and SRN2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRN2Q991766EBI-23478,EBI-18076
STP22P256046EBI-23478,EBI-411625

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi33459. 29 interactors.
DIPiDIP-5332N.
IntActiP42939. 3 interactors.
MINTiMINT-508989.

Structurei

Secondary structure

1101
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 11Combined sources8
Beta strandi15 – 19Combined sources5
Helixi41 – 65Combined sources25
Helixi67 – 78Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P22X-ray2.70D4-81[»]
ProteinModelPortaliP42939.
SMRiP42939.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42939.

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000939.
InParanoidiP42939.
KOiK12187.
OMAiEECDNIT.
OrthoDBiEOG092C5KPY.

Family and domain databases

InterProiIPR019014. Mvb12.
[Graphical view]
PfamiPF09452. Mvb12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNVEELLR RIPLYNKYGK DFPQETVTRF QMPEFKLPAL QPTRDLLCPW
60 70 80 90 100
YEECDNITKV CQLHDSSNKK FDQWYKEQYL SKKPPGIVGN TLLSPSRKDN

S
Length:101
Mass (Da):11,968
Last modified:November 1, 1995 - v1
Checksum:iE48B085E0FACBAC8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88999.1.
Z72991 Genomic DNA. Translation: CAA97233.1.
AY692570 Genomic DNA. Translation: AAT92589.1.
BK006941 Genomic DNA. Translation: DAA08300.1.
PIRiS53929.
RefSeqiNP_011722.3. NM_001181335.3.

Genome annotation databases

EnsemblFungiiYGR206W; YGR206W; YGR206W.
GeneIDi853120.
KEGGisce:YGR206W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88999.1.
Z72991 Genomic DNA. Translation: CAA97233.1.
AY692570 Genomic DNA. Translation: AAT92589.1.
BK006941 Genomic DNA. Translation: DAA08300.1.
PIRiS53929.
RefSeqiNP_011722.3. NM_001181335.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P22X-ray2.70D4-81[»]
ProteinModelPortaliP42939.
SMRiP42939.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33459. 29 interactors.
DIPiDIP-5332N.
IntActiP42939. 3 interactors.
MINTiMINT-508989.

PTM databases

iPTMnetiP42939.

Proteomic databases

MaxQBiP42939.
PRIDEiP42939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR206W; YGR206W; YGR206W.
GeneIDi853120.
KEGGisce:YGR206W.

Organism-specific databases

EuPathDBiFungiDB:YGR206W.
SGDiS000003438. MVB12.

Phylogenomic databases

HOGENOMiHOG000000939.
InParanoidiP42939.
KOiK12187.
OMAiEECDNIT.
OrthoDBiEOG092C5KPY.

Enzyme and pathway databases

BioCyciYEAST:G3O-30889-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP42939.
PROiP42939.

Family and domain databases

InterProiIPR019014. Mvb12.
[Graphical view]
PfamiPF09452. Mvb12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMVB12_YEAST
AccessioniPrimary (citable) accession number: P42939
Secondary accession number(s): D6VUY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.