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P42939

- MVB12_YEAST

UniProt

P42939 - MVB12_YEAST

Protein

Multivesicular body sorting factor 12

Gene

MVB12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Appears to be involved in cargo sorting and release of the ESCRT-I complex from the MVBs.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin binding Source: SGD

    GO - Biological processi

    1. endosome transport via multivesicular body sorting pathway Source: SGD
    2. negative regulation of protein complex assembly Source: SGD
    3. protein targeting to vacuole Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30889-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multivesicular body sorting factor 12
    Alternative name(s):
    12 kDa multivesicular body sorting factor
    ESCRT-I complex subunit MVB12
    Gene namesi
    Name:MVB12
    Ordered Locus Names:YGR206W
    ORF Names:G7740
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR206w.
    SGDiS000003438. MVB12.

    Subcellular locationi

    Cytoplasm 1 Publication. Endosome 1 Publication. Late endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD
    2. endosome Source: SGD
    3. ESCRT I complex Source: SGD
    4. late endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi47 – 471L → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57.
    Mutagenesisi54 – 541C → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57. 1 Publication
    Mutagenesisi57 – 571I → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47. 1 Publication
    Mutagenesisi64 – 641H → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 101101Multivesicular body sorting factor 12PRO_0000202845Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei94 – 941Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP42939.
    PaxDbiP42939.

    Expressioni

    Gene expression databases

    GenevestigatoriP42939.

    Interactioni

    Subunit structurei

    Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with STP22 and SRN2.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SRN2Q991766EBI-23478,EBI-18076
    STP22P256046EBI-23478,EBI-411625

    Protein-protein interaction databases

    BioGridi33459. 29 interactions.
    DIPiDIP-5332N.
    IntActiP42939. 3 interactions.
    MINTiMINT-508989.
    STRINGi4932.YGR206W.

    Structurei

    Secondary structure

    1
    101
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 118
    Beta strandi15 – 195
    Helixi41 – 6525
    Helixi67 – 7812

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2P22X-ray2.70D4-81[»]
    ProteinModelPortaliP42939.
    SMRiP42939. Positions 4-81.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP42939.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG71344.
    HOGENOMiHOG000000939.
    KOiK12187.
    OMAiHEANRIK.
    OrthoDBiEOG72C5D4.

    Family and domain databases

    InterProiIPR019014. ESCRT-I_cplx_su_Mvb12.
    [Graphical view]
    PfamiPF09452. Mvb12. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42939-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNNVEELLR RIPLYNKYGK DFPQETVTRF QMPEFKLPAL QPTRDLLCPW    50
    YEECDNITKV CQLHDSSNKK FDQWYKEQYL SKKPPGIVGN TLLSPSRKDN 100
    S 101
    Length:101
    Mass (Da):11,968
    Last modified:November 1, 1995 - v1
    Checksum:iE48B085E0FACBAC8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49133 Genomic DNA. Translation: CAA88999.1.
    Z72991 Genomic DNA. Translation: CAA97233.1.
    AY692570 Genomic DNA. Translation: AAT92589.1.
    BK006941 Genomic DNA. Translation: DAA08300.1.
    PIRiS53929.
    RefSeqiNP_011722.3. NM_001181335.3.

    Genome annotation databases

    EnsemblFungiiYGR206W; YGR206W; YGR206W.
    GeneIDi853120.
    KEGGisce:YGR206W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49133 Genomic DNA. Translation: CAA88999.1 .
    Z72991 Genomic DNA. Translation: CAA97233.1 .
    AY692570 Genomic DNA. Translation: AAT92589.1 .
    BK006941 Genomic DNA. Translation: DAA08300.1 .
    PIRi S53929.
    RefSeqi NP_011722.3. NM_001181335.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2P22 X-ray 2.70 D 4-81 [» ]
    ProteinModelPortali P42939.
    SMRi P42939. Positions 4-81.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33459. 29 interactions.
    DIPi DIP-5332N.
    IntActi P42939. 3 interactions.
    MINTi MINT-508989.
    STRINGi 4932.YGR206W.

    Proteomic databases

    MaxQBi P42939.
    PaxDbi P42939.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR206W ; YGR206W ; YGR206W .
    GeneIDi 853120.
    KEGGi sce:YGR206W.

    Organism-specific databases

    CYGDi YGR206w.
    SGDi S000003438. MVB12.

    Phylogenomic databases

    eggNOGi NOG71344.
    HOGENOMi HOG000000939.
    KOi K12187.
    OMAi HEANRIK.
    OrthoDBi EOG72C5D4.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30889-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P42939.
    NextBioi 973154.

    Gene expression databases

    Genevestigatori P42939.

    Family and domain databases

    InterProi IPR019014. ESCRT-I_cplx_su_Mvb12.
    [Graphical view ]
    Pfami PF09452. Mvb12. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs."
      Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C.
      Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Structural insight into the ESCRT-I/-II link and its role in MVB trafficking."
      Gill D.J., Teo H., Sun J., Perisic O., Veprintsev D.B., Emr S.D., Williams R.L.
      EMBO J. 26:600-612(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ESCRT-I COMPLEX, COMPOSITION OF THE ESCRT-I COMPLEX.
    7. "Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12."
      Curtiss M., Jones C., Babst M.
      Mol. Biol. Cell 18:636-645(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ESCRT-I COMPLEX.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Molecular architecture and functional model of the complete yeast ESCRT-I heterotetramer."
      Kostelansky M.S., Schluter C., Tam Y.Y., Lee S., Ghirlando R., Beach B., Conibear E., Hurley J.H.
      Cell 129:485-498(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 4-81, COMPOSITION OF THE ESCRT-I COMPLEX, INTERACTION WITH STP22 AND SRN2, MUTAGENESIS OF CYS-54; ILE-57 AND HIS-64.

    Entry informationi

    Entry nameiMVB12_YEAST
    AccessioniPrimary (citable) accession number: P42939
    Secondary accession number(s): D6VUY9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 752 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3