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P42939 (MVB12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multivesicular body sorting factor 12
Alternative name(s):
12 kDa multivesicular body sorting factor
ESCRT-I complex subunit MVB12
Gene names
Name:MVB12
Ordered Locus Names:YGR206W
ORF Names:G7740
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length101 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Appears to be involved in cargo sorting and release of the ESCRT-I complex from the MVBs. Ref.7

Subunit structure

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with STP22 and SRN2. Ref.6 Ref.7 Ref.10

Subcellular location

Cytoplasm. Endosome. Late endosome membrane; Peripheral membrane protein Probable Ref.7.

Miscellaneous

Present with 752 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SRN2Q991766EBI-23478,EBI-18076
STP22P256046EBI-23478,EBI-411625

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 101101Multivesicular body sorting factor 12
PRO_0000202845

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9
Modified residue941Phosphoserine Ref.8

Experimental info

Mutagenesis471L → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57.
Mutagenesis541C → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57. Ref.10
Mutagenesis571I → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47. Ref.10
Mutagenesis641H → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47. Ref.10

Secondary structure

......... 101
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42939 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E48B085E0FACBAC8

FASTA10111,968
        10         20         30         40         50         60 
MNNNVEELLR RIPLYNKYGK DFPQETVTRF QMPEFKLPAL QPTRDLLCPW YEECDNITKV 

        70         80         90        100 
CQLHDSSNKK FDQWYKEQYL SKKPPGIVGN TLLSPSRKDN S 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs."
Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C.
Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Structural insight into the ESCRT-I/-II link and its role in MVB trafficking."
Gill D.J., Teo H., Sun J., Perisic O., Veprintsev D.B., Emr S.D., Williams R.L.
EMBO J. 26:600-612(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ESCRT-I COMPLEX, COMPOSITION OF THE ESCRT-I COMPLEX.
[7]"Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12."
Curtiss M., Jones C., Babst M.
Mol. Biol. Cell 18:636-645(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ESCRT-I COMPLEX.
[8]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Molecular architecture and functional model of the complete yeast ESCRT-I heterotetramer."
Kostelansky M.S., Schluter C., Tam Y.Y., Lee S., Ghirlando R., Beach B., Conibear E., Hurley J.H.
Cell 129:485-498(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 4-81, COMPOSITION OF THE ESCRT-I COMPLEX, INTERACTION WITH STP22 AND SRN2, MUTAGENESIS OF CYS-54; ILE-57 AND HIS-64.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49133 Genomic DNA. Translation: CAA88999.1.
Z72991 Genomic DNA. Translation: CAA97233.1.
AY692570 Genomic DNA. Translation: AAT92589.1.
BK006941 Genomic DNA. Translation: DAA08300.1.
PIRS53929.
RefSeqNP_011722.3. NM_001181335.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2P22X-ray2.70D4-81[»]
ProteinModelPortalP42939.
SMRP42939. Positions 4-81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33459. 29 interactions.
DIPDIP-5332N.
IntActP42939. 3 interactions.
MINTMINT-508989.
STRING4932.YGR206W.

Proteomic databases

PaxDbP42939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR206W; YGR206W; YGR206W.
GeneID853120.
KEGGsce:YGR206W.

Organism-specific databases

CYGDYGR206w.
SGDS000003438. MVB12.

Phylogenomic databases

eggNOGNOG71344.
HOGENOMHOG000000939.
KOK12187.
OMAEECDNIT.
OrthoDBEOG72C5D4.

Enzyme and pathway databases

BioCycYEAST:G3O-30889-MONOMER.

Gene expression databases

GenevestigatorP42939.

Family and domain databases

InterProIPR019014. ESCRT-I_cplx_su_Mvb12.
[Graphical view]
PfamPF09452. Mvb12. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP42939.
NextBio973154.

Entry information

Entry nameMVB12_YEAST
AccessionPrimary (citable) accession number: P42939
Secondary accession number(s): D6VUY9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references