Multivesicular body sorting factor 12 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P42939 (MVB12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Multivesicular body sorting factor 12

Alternative name(s):
12 kDa multivesicular body sorting factor
ESCRT-I complex subunit MVB12

Gene names

Name:	MVB12
Ordered Locus Names:	YGR206W
ORF Names:	G7740

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Appears to be involved in cargo sorting and release of the ESCRT-I complex from the MVBs. Ref.7
Subunit structure	Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with STP22 and SRN2. Ref.6 Ref.7 Ref.9
Subcellular location	Cytoplasm. Endosome. Late endosome membrane; Peripheral membrane protein Probable Ref.7.
Miscellaneous	Present with 752 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endosome transport via multivesicular body sorting pathway Inferred by curator PubMed 17145965. Source: SGD negative regulation of protein complex assembly Inferred from mutant phenotype PubMed 17145965. Source: SGD protein targeting to vacuole Inferred from mutant phenotype Ref.7 PubMed 17145965. Source: SGD
Cellular_component	ESCRT I complex Inferred from direct assay Ref.7 PubMed 17145965. Source: SGD cytosol Inferred from direct assay PubMed 17145965. Source: SGD late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin binding Inferred from direct assay PubMed 19380877. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRN2	Q99176	4	EBI-23478,EBI-18076
STP22	P25604	6	EBI-23478,EBI-411625

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 101

101

Multivesicular body sorting factor 12

PRO_0000202845

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Experimental info

Mutagenesis

L → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-57.

Mutagenesis

C → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-57. Ref.9

Mutagenesis

I → D: Defective in MVB12 incorporation in ESCRT-I complex; reduces localization to MVBs; abolishes interaction with STP22 and SRN2; when associated with D-47. Ref.9

Mutagenesis

H → D: Defective in MVB12 incorporation in ESCRT-I complex; when associated with D-47. Ref.9

Secondary structure

101

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P42939 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E48B085E0FACBAC8
Show »

FASTA

101

11,968

        10         20         30         40         50         60 
MNNNVEELLR RIPLYNKYGK DFPQETVTRF QMPEFKLPAL QPTRDLLCPW YEECDNITKV 

        70         80         90        100 
CQLHDSSNKK FDQWYKEQYL SKKPPGIVGN TLLSPSRKDN S

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs." Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C. Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structural insight into the ESCRT-I/-II link and its role in MVB trafficking." Gill D.J., Teo H., Sun J., Perisic O., Veprintsev D.B., Emr S.D., Williams R.L. EMBO J. 26:600-612(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE ESCRT-I COMPLEX, COMPOSITION OF THE ESCRT-I COMPLEX.
[7]	"Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12." Curtiss M., Jones C., Babst M. Mol. Biol. Cell 18:636-645(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ESCRT-I COMPLEX.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, MASS SPECTROMETRY.
[9]	"Molecular architecture and functional model of the complete yeast ESCRT-I heterotetramer." Kostelansky M.S., Schluter C., Tam Y.Y., Lee S., Ghirlando R., Beach B., Conibear E., Hurley J.H. Cell 129:485-498(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 4-81, COMPOSITION OF THE ESCRT-I COMPLEX, INTERACTION WITH STP22 AND SRN2, MUTAGENESIS OF CYS-54; ILE-57 AND HIS-64.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49133 Genomic DNA. Translation: CAA88999.1.
Z72991 Genomic DNA. Translation: CAA97233.1.
AY692570 Genomic DNA. Translation: AAT92589.1.
BK006941 Genomic DNA. Translation: DAA08300.1.

PIR

S53929.

RefSeq

NP_011722.3. NM_001181335.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2P22	X-ray	2.70	D	4-81	[»]

ProteinModelPortal

P42939.

SMR

P42939. Positions 4-81.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5332N.

IntAct

P42939. 3 interactions.

MINT

MINT-508989.

STRING

4932.YGR206W.

Proteomic databases

PaxDb

P42939.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR206W; YGR206W; YGR206W.

GeneID

853120.

KEGG

sce:YGR206W.
sce:YGR212W.

Organism-specific databases

CYGD

YGR206w.

SGD

S000003438. MVB12.

Phylogenomic databases

eggNOG

NOG71344.

HOGENOM

HOG000000939.

K12187.

OMA

EECDNIT.

OrthoDB

EOG4HQHV7.

Gene expression databases

Genevestigator

P42939.

GermOnline

YGR206W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR019014. ESCRT-I_cplx_su_Mvb12.
[Graphical view]

Pfam

PF09452. Mvb12. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P42939.

NextBio

973154.

Entry information

Entry name

MVB12_YEAST

Accession

Primary (citable) accession number: P42939
Secondary accession number(s): D6VUY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents