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Protein

Elongator complex protein 2

Gene

ELP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP2 is dispensable for the complex integrity and, in vitro, is not required for complex HAT activity. It is not required for the association of the complex with nascent RNA transcript. Independently, ELP2 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.8 Publications

GO - Molecular functioni

  • microtubule binding Source: SGD

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • protein urmylation Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA wobble uridine modification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30885-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 2
Alternative name(s):
Gamma-toxin target 2
Gene namesi
Name:ELP2
Synonyms:TOT2
Ordered Locus Names:YGR200C
ORF Names:G7725
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR200C.
SGDiS000003432. ELP2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • Elongator holoenzyme complex Source: SGD
  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514741 – 788Elongator complex protein 2Add BLAST788

Proteomic databases

MaxQBiP42935.
PRIDEiP42935.

PTM databases

iPTMnetiP42935.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, ELP2 interacts with IKI3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELP4Q028847EBI-23459,EBI-35277
IKI1P388746EBI-23459,EBI-9061

GO - Molecular functioni

  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi33453. 290 interactors.
DIPiDIP-2386N.
IntActiP42935. 16 interactors.
MINTiMINT-613845.

Structurei

Secondary structure

1788
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 12Combined sources8
Beta strandi21 – 23Combined sources3
Turni25 – 27Combined sources3
Beta strandi30 – 34Combined sources5
Beta strandi37 – 41Combined sources5
Beta strandi51 – 56Combined sources6
Beta strandi62 – 67Combined sources6
Beta strandi71 – 81Combined sources11
Beta strandi83 – 92Combined sources10
Beta strandi94 – 100Combined sources7
Beta strandi109 – 111Combined sources3
Beta strandi116 – 120Combined sources5
Beta strandi124 – 131Combined sources8
Beta strandi133 – 145Combined sources13
Beta strandi153 – 160Combined sources8
Beta strandi162 – 183Combined sources22
Beta strandi190 – 198Combined sources9
Beta strandi205 – 212Combined sources8
Beta strandi214 – 217Combined sources4
Beta strandi219 – 228Combined sources10
Beta strandi230 – 236Combined sources7
Beta strandi258 – 261Combined sources4
Beta strandi269 – 276Combined sources8
Beta strandi284 – 289Combined sources6
Beta strandi291 – 294Combined sources4
Beta strandi296 – 309Combined sources14
Beta strandi318 – 324Combined sources7
Beta strandi343 – 348Combined sources6
Beta strandi353 – 358Combined sources6
Beta strandi384 – 386Combined sources3
Beta strandi388 – 393Combined sources6
Beta strandi400 – 404Combined sources5
Beta strandi409 – 415Combined sources7
Beta strandi428 – 436Combined sources9
Beta strandi442 – 449Combined sources8
Beta strandi452 – 466Combined sources15
Helixi469 – 478Combined sources10
Helixi540 – 543Combined sources4
Beta strandi550 – 554Combined sources5
Beta strandi561 – 566Combined sources6
Beta strandi570 – 577Combined sources8
Turni582 – 584Combined sources3
Beta strandi587 – 591Combined sources5
Turni592 – 594Combined sources3
Beta strandi611 – 614Combined sources4
Beta strandi618 – 624Combined sources7
Beta strandi626 – 628Combined sources3
Beta strandi630 – 635Combined sources6
Turni637 – 639Combined sources3
Beta strandi642 – 649Combined sources8
Beta strandi656 – 661Combined sources6
Turni664 – 666Combined sources3
Beta strandi668 – 674Combined sources7
Beta strandi677 – 685Combined sources9
Beta strandi687 – 699Combined sources13
Beta strandi704 – 709Combined sources6
Beta strandi714 – 726Combined sources13
Beta strandi729 – 735Combined sources7
Beta strandi738 – 744Combined sources7
Turni747 – 749Combined sources3
Beta strandi755 – 764Combined sources10
Beta strandi769 – 775Combined sources7
Beta strandi778 – 785Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XFVX-ray3.20A1-788[»]
ProteinModelPortaliP42935.
SMRiP42935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 87WD 1Add BLAST31
Repeati101 – 130WD 2Add BLAST30
Repeati141 – 181WD 3Add BLAST41
Repeati200 – 234WD 4Add BLAST35
Repeati279 – 309WD 5Add BLAST31
Repeati383 – 413WD 6Add BLAST31
Repeati438 – 466WD 7Add BLAST29
Repeati604 – 634WD 8Add BLAST31
Repeati651 – 683WD 9Add BLAST33

Sequence similaritiesi

Belongs to the WD repeat ELP2 family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000000916.
HOGENOMiHOG000200623.
InParanoidiP42935.
KOiK11374.
OMAiESIRIWR.
OrthoDBiEOG092C09SX.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVECITPEAI FIGANKQTQV SDIHKVKKIV AFGAGKTIAL WDPIEPNNKG
60 70 80 90 100
VYATLKGHEA EVTCVRFVPD SDFMVSASED HHVKIWKFTD YSHLQCIQTI
110 120 130 140 150
QHYSKTIVAL SALPSLISVG CADGTISIWR QNIQNDEFGL AHEFTIKKGF
160 170 180 190 200
FYPLCLSLSK VEEKKYLLAI GGTNVNVFIA SFILSDSGIE KCRVVAELEG
210 220 230 240 250
HEDWVKSLAF RHQETPGDYL LCSGSQDRYI RLWRIRINDL IDDSEEDSKK
260 270 280 290 300
LTLLSNKQYK FQIDDELRVG INFEALIMGH DDWISSLQWH ESRLQLLAAT
310 320 330 340 350
ADTSLMVWEP DETSGIWVCS LRLGEMSSKG ASTATGSSGG FWSCLWFTHE
360 370 380 390 400
RMDFFLTNGK TGSWRMWATK DNIICDQRLG ISGATKDVTD IAWSPSGEYL
410 420 430 440 450
LATSLDQTTR LFAPWIYDAS GRKREIATWH EFSRPQIHGY DMICVETVTD
460 470 480 490 500
TRFVSGGDEK ILRSFDLPKG VAGMLQKFVG IQFEEKSEMP DSATVPVLGL
510 520 530 540 550
SNKAGEDDAN EDDEEEEGGN KETPDITDPL SLLECPPMED QLQRHLLWPE
560 570 580 590 600
VEKLYGHGFE ITCLDISPDQ KLIASACRSN NVQNAVIRIF STENWLEIKP
610 620 630 640 650
ALPFHSLTIT RLKFSKDGKF LLSVCRDRKW ALWERNMEDN TFELRFKNEK
660 670 680 690 700
PHTRIIWDAD WAPLEFGNVF VTASRDKTVK VWRHQKEPAD DYVLEASIKH
710 720 730 740 750
TKAVTAISIH DSMIREKILI SVGLENGEIY LYSYTLGKFE LITQLNEDIT
760 770 780
PADKITRLRW SHLKRNGKLF LGVGSSDLST RIYSLAYE
Length:788
Mass (Da):89,411
Last modified:November 1, 1995 - v1
Checksum:i5371908FE2E6EC0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti579S → P in AAS56426 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88993.1.
Z72985 Genomic DNA. Translation: CAA97227.1.
AY558100 Genomic DNA. Translation: AAS56426.1.
BK006941 Genomic DNA. Translation: DAA08293.1.
PIRiS53923.
RefSeqiNP_011716.3. NM_001181329.3.

Genome annotation databases

EnsemblFungiiYGR200C; YGR200C; YGR200C.
GeneIDi853114.
KEGGisce:YGR200C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88993.1.
Z72985 Genomic DNA. Translation: CAA97227.1.
AY558100 Genomic DNA. Translation: AAS56426.1.
BK006941 Genomic DNA. Translation: DAA08293.1.
PIRiS53923.
RefSeqiNP_011716.3. NM_001181329.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XFVX-ray3.20A1-788[»]
ProteinModelPortaliP42935.
SMRiP42935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33453. 290 interactors.
DIPiDIP-2386N.
IntActiP42935. 16 interactors.
MINTiMINT-613845.

PTM databases

iPTMnetiP42935.

Proteomic databases

MaxQBiP42935.
PRIDEiP42935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR200C; YGR200C; YGR200C.
GeneIDi853114.
KEGGisce:YGR200C.

Organism-specific databases

EuPathDBiFungiDB:YGR200C.
SGDiS000003432. ELP2.

Phylogenomic databases

GeneTreeiENSGT00390000000916.
HOGENOMiHOG000200623.
InParanoidiP42935.
KOiK11374.
OMAiESIRIWR.
OrthoDBiEOG092C09SX.

Enzyme and pathway databases

BioCyciYEAST:G3O-30885-MONOMER.

Miscellaneous databases

PROiP42935.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 11 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiPS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELP2_YEAST
AccessioniPrimary (citable) accession number: P42935
Secondary accession number(s): D6VUY2, E9P8V1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.