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Protein

Elongator complex protein 2

Gene

ELP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP2 is dispensable for the complex integrity and, in vitro, is not required for complex HAT activity. It is not required for the association of the complex with nascent RNA transcript. Independently, ELP2 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA.8 Publications

Miscellaneous

Present with 6090 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • dynein complex binding Source: GO_Central
  • microtubule binding Source: SGD

GO - Biological processi

  • protein transport Source: UniProtKB-KW
  • protein urmylation Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • tRNA wobble uridine modification Source: SGD

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30885-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongator complex protein 2
Alternative name(s):
Gamma-toxin target 2
Gene namesi
Name:ELP2
Synonyms:TOT2
Ordered Locus Names:YGR200C
ORF Names:G7725
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR200C.
SGDiS000003432. ELP2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514741 – 788Elongator complex protein 2Add BLAST788

Proteomic databases

MaxQBiP42935.
PRIDEiP42935.

PTM databases

iPTMnetiP42935.

Interactioni

Subunit structurei

Component of the RNA polymerase II elongator complex, which consists of IKI3, ELP2, ELP3, ELP4, IKI1 and ELP6. IKI3, ELP2, and ELP3 form the elongator core complex. In the complex, ELP2 interacts with IKI3.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • dynein complex binding Source: GO_Central
  • microtubule binding Source: SGD

Protein-protein interaction databases

BioGridi33453. 503 interactors.
DIPiDIP-2386N.
IntActiP42935. 19 interactors.
MINTiMINT-613845.
STRINGi4932.YGR200C.

Structurei

Secondary structure

1788
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Beta strandi20 – 23Combined sources4
Turni25 – 27Combined sources3
Beta strandi30 – 34Combined sources5
Beta strandi37 – 41Combined sources5
Beta strandi51 – 55Combined sources5
Beta strandi62 – 67Combined sources6
Beta strandi69 – 78Combined sources10
Beta strandi83 – 92Combined sources10
Beta strandi94 – 100Combined sources7
Beta strandi107 – 113Combined sources7
Beta strandi116 – 121Combined sources6
Beta strandi124 – 131Combined sources8
Beta strandi133 – 136Combined sources4
Beta strandi138 – 145Combined sources8
Beta strandi148 – 150Combined sources3
Beta strandi153 – 162Combined sources10
Beta strandi165 – 185Combined sources21
Beta strandi188 – 198Combined sources11
Beta strandi205 – 210Combined sources6
Beta strandi214 – 217Combined sources4
Beta strandi218 – 225Combined sources8
Beta strandi228 – 239Combined sources12
Beta strandi259 – 264Combined sources6
Beta strandi267 – 278Combined sources12
Beta strandi280 – 289Combined sources10
Beta strandi291 – 294Combined sources4
Beta strandi296 – 300Combined sources5
Beta strandi303 – 310Combined sources8
Turni312 – 314Combined sources3
Beta strandi317 – 324Combined sources8
Beta strandi344 – 349Combined sources6
Beta strandi352 – 358Combined sources7
Beta strandi364 – 373Combined sources10
Beta strandi376 – 381Combined sources6
Beta strandi384 – 386Combined sources3
Beta strandi388 – 393Combined sources6
Beta strandi398 – 404Combined sources7
Turni405 – 407Combined sources3
Beta strandi408 – 414Combined sources7
Beta strandi429 – 438Combined sources10
Beta strandi442 – 457Combined sources16
Beta strandi460 – 466Combined sources7
Helixi469 – 479Combined sources11
Helixi539 – 544Combined sources6
Beta strandi550 – 555Combined sources6
Beta strandi561 – 566Combined sources6
Beta strandi572 – 577Combined sources6
Beta strandi581 – 583Combined sources3
Beta strandi586 – 591Combined sources6
Turni592 – 594Combined sources3
Beta strandi597 – 603Combined sources7
Beta strandi609 – 614Combined sources6
Beta strandi618 – 625Combined sources8
Beta strandi626 – 628Combined sources3
Beta strandi630 – 636Combined sources7
Turni637 – 640Combined sources4
Beta strandi641 – 651Combined sources11
Beta strandi656 – 661Combined sources6
Helixi664 – 666Combined sources3
Beta strandi668 – 674Combined sources7
Beta strandi677 – 685Combined sources9
Beta strandi687 – 699Combined sources13
Beta strandi704 – 709Combined sources6
Beta strandi717 – 724Combined sources8
Beta strandi729 – 735Combined sources7
Beta strandi738 – 744Combined sources7
Turni747 – 749Combined sources3
Beta strandi755 – 760Combined sources6
Beta strandi768 – 775Combined sources8
Beta strandi780 – 786Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XFVX-ray3.20A1-788[»]
5M2NX-ray2.81A1-788[»]
ProteinModelPortaliP42935.
SMRiP42935.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 87WD 1Add BLAST31
Repeati101 – 130WD 2Add BLAST30
Repeati141 – 181WD 3Add BLAST41
Repeati200 – 234WD 4Add BLAST35
Repeati279 – 309WD 5Add BLAST31
Repeati383 – 413WD 6Add BLAST31
Repeati438 – 466WD 7Add BLAST29
Repeati604 – 634WD 8Add BLAST31
Repeati651 – 683WD 9Add BLAST33

Sequence similaritiesi

Belongs to the WD repeat ELP2 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000000916.
HOGENOMiHOG000200623.
InParanoidiP42935.
KOiK11374.
OMAiLHLWKQN.
OrthoDBiEOG092C09SX.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiView protein in InterPro
IPR037289. Elp2.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PANTHERiPTHR44111. PTHR44111. 1 hit.
PfamiView protein in Pfam
PF00400. WD40. 5 hits.
SMARTiView protein in SMART
SM00320. WD40. 11 hits.
SUPFAMiSSF50978. SSF50978. 4 hits.
PROSITEiView protein in PROSITE
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 3 hits.

Sequencei

Sequence statusi: Complete.

P42935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVECITPEAI FIGANKQTQV SDIHKVKKIV AFGAGKTIAL WDPIEPNNKG
60 70 80 90 100
VYATLKGHEA EVTCVRFVPD SDFMVSASED HHVKIWKFTD YSHLQCIQTI
110 120 130 140 150
QHYSKTIVAL SALPSLISVG CADGTISIWR QNIQNDEFGL AHEFTIKKGF
160 170 180 190 200
FYPLCLSLSK VEEKKYLLAI GGTNVNVFIA SFILSDSGIE KCRVVAELEG
210 220 230 240 250
HEDWVKSLAF RHQETPGDYL LCSGSQDRYI RLWRIRINDL IDDSEEDSKK
260 270 280 290 300
LTLLSNKQYK FQIDDELRVG INFEALIMGH DDWISSLQWH ESRLQLLAAT
310 320 330 340 350
ADTSLMVWEP DETSGIWVCS LRLGEMSSKG ASTATGSSGG FWSCLWFTHE
360 370 380 390 400
RMDFFLTNGK TGSWRMWATK DNIICDQRLG ISGATKDVTD IAWSPSGEYL
410 420 430 440 450
LATSLDQTTR LFAPWIYDAS GRKREIATWH EFSRPQIHGY DMICVETVTD
460 470 480 490 500
TRFVSGGDEK ILRSFDLPKG VAGMLQKFVG IQFEEKSEMP DSATVPVLGL
510 520 530 540 550
SNKAGEDDAN EDDEEEEGGN KETPDITDPL SLLECPPMED QLQRHLLWPE
560 570 580 590 600
VEKLYGHGFE ITCLDISPDQ KLIASACRSN NVQNAVIRIF STENWLEIKP
610 620 630 640 650
ALPFHSLTIT RLKFSKDGKF LLSVCRDRKW ALWERNMEDN TFELRFKNEK
660 670 680 690 700
PHTRIIWDAD WAPLEFGNVF VTASRDKTVK VWRHQKEPAD DYVLEASIKH
710 720 730 740 750
TKAVTAISIH DSMIREKILI SVGLENGEIY LYSYTLGKFE LITQLNEDIT
760 770 780
PADKITRLRW SHLKRNGKLF LGVGSSDLST RIYSLAYE
Length:788
Mass (Da):89,411
Last modified:November 1, 1995 - v1
Checksum:i5371908FE2E6EC0D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti579S → P in AAS56426 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88993.1.
Z72985 Genomic DNA. Translation: CAA97227.1.
AY558100 Genomic DNA. Translation: AAS56426.1.
BK006941 Genomic DNA. Translation: DAA08293.1.
PIRiS53923.
RefSeqiNP_011716.3. NM_001181329.3.

Genome annotation databases

EnsemblFungiiYGR200C; YGR200C; YGR200C.
GeneIDi853114.
KEGGisce:YGR200C.

Similar proteinsi

Entry informationi

Entry nameiELP2_YEAST
AccessioniPrimary (citable) accession number: P42935
Secondary accession number(s): D6VUY2, E9P8V1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names