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Protein

Dolichyl-phosphate-mannose--protein mannosyltransferase 6

Gene

PMT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Protein O-mannosyltransferase involved in O-glycosylation which is essential for cell wall rigidity. Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.2 PublicationsBy similarity

Catalytic activityi

Dolichyl D-mannosyl phosphate + protein = dolichyl phosphate + O-D-mannosylprotein.2 PublicationsBy similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • ER-associated misfolded protein catabolic process Source: GO_Central
  • fungal-type cell wall organization Source: GO_Central
  • protein O-linked glycosylation Source: SGD
  • protein O-linked mannosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YGR199W-MONOMER.
BRENDAi2.4.1.109. 984.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 6Curated (EC:2.4.1.1092 PublicationsBy similarity)
Gene namesi
Name:PMT6Imported
Ordered Locus Names:YGR199WImported
ORF Names:G77221 Publication
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR199W.
SGDiS000003431. PMT6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 58CytoplasmicSequence analysisAdd BLAST57
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Topological domaini80 – 164LumenalSequence analysisAdd BLAST85
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 194CytoplasmicSequence analysis9
Transmembranei195 – 215HelicalSequence analysisAdd BLAST21
Topological domaini216 – 251LumenalSequence analysisAdd BLAST36
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Topological domaini273 – 293CytoplasmicSequence analysisAdd BLAST21
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Topological domaini315 – 618LumenalSequence analysisAdd BLAST304
Transmembranei619 – 639HelicalSequence analysisAdd BLAST21
Topological domaini640 – 656CytoplasmicSequence analysisAdd BLAST17
Transmembranei657 – 677HelicalSequence analysisAdd BLAST21
Topological domaini678 – 686LumenalSequence analysis9
Transmembranei687 – 707HelicalSequence analysisAdd BLAST21
Topological domaini708 – 715CytoplasmicSequence analysis8
Transmembranei716 – 736HelicalSequence analysisAdd BLAST21
Topological domaini737 – 759LumenalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Affects GAS1 and GGP1 O-mannosylation.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001214962 – 759Dolichyl-phosphate-mannose--protein mannosyltransferase 6Add BLAST758

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Glycosylationi404N-linked (GlcNAc...)Sequence analysis1
Glycosylationi481N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

MaxQBiP42934.
PRIDEiP42934.

PTM databases

iPTMnetiP42934.

Interactioni

Subunit structurei

May form a hetodimer with PMT4.1 Publication

Protein-protein interaction databases

BioGridi33452. 47 interactors.
DIPiDIP-5551N.
IntActiP42934. 1 interactor.
MINTiMINT-561799.

Structurei

3D structure databases

ProteinModelPortaliP42934.
SMRiP42934.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini340 – 394MIR 1PROSITE-ProRule annotationAdd BLAST55
Domaini409 – 467MIR 2PROSITE-ProRule annotationAdd BLAST59
Domaini482 – 540MIR 3PROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Belongs to the glycosyltransferase 39 family.Curated
Contains 3 MIR domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133869.
HOGENOMiHOG000157526.
InParanoidiP42934.
KOiK00728.
OMAiSTWWNVE.
OrthoDBiEOG092C0JF4.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42934-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAKGTGFS SIDTEDENLR ERYVNQPKAN ASDIQDEQLD CFEQLEEKHR
60 70 80 90 100
TKKNEEYTAL KILRDVIGPL LLTITSFYLR FQHIDQNNYV VWDEAHFGKF
110 120 130 140 150
GSYYIKHEYY HDVHPPLGKM LIALSEWMAG FDGQFDFSSN NAYPENVNFK
160 170 180 190 200
LMRQFNATFG ALCTPVAFFT AKWMGFNYFT VYLIATMVTL EHSYIVLSKF
210 220 230 240 250
ILLDSMLLFF SMTTFACMIK LYTLRKQQMT KKWSLWMLLT GLSIGCVCSV
260 270 280 290 300
KWVGLFITVV VGLYTCIELF LLYCDKELPR IKYYKHWLIR IINLIVIPFL
310 320 330 340 350
IYLYCFKIHF VLLYKSGTGD STTNTLFQIN LEGTQIEAGP RDVAFGSELT
360 370 380 390 400
IRSHGLSPNL LHSHIQVYPE GSGQRQITGY GFADSNNVWK FEFSRSSGLE
410 420 430 440 450
LDQNGTLNGK IIPITDGVEV RLSHKNTGSN LHSHDVPSHV SRGNYEVSGY
460 470 480 490 500
GSQSVGDEKD DWIVEIVKQM DSPNPVYSNE NSTILHPVST FFRLRHKVLG
510 520 530 540 550
CYLASTGLTY PAWGFKQAEI VCKDSWSRRD KSTWWNVEDH WNHNLETAED
560 570 580 590 600
YVPPKSNFWT DFILTNFAMA SSNNALVPDE DKYDSLSSDA WEWPTLHKGL
610 620 630 640 650
RMCSWAGYIT RYYLMGSPFN TWISTVSLII FPFIILFILY RWRRQTLYLS
660 670 680 690 700
DDQIWQITIQ GIFPFISWMT HYLPFAMMGR VTYVHHYVPA LYFAMLVFGF
710 720 730 740 750
VLDFTLTRVH WMVKYPIYLS LFGGCIYIYN LFAPICQGMH GDKAEYLPLQ

WLSTWDIAP
Length:759
Mass (Da):88,026
Last modified:November 1, 1995 - v1
Checksum:i7A23DCEDD90F01C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88992.1.
Z72984 Genomic DNA. Translation: CAA97226.1.
BK006941 Genomic DNA. Translation: DAA08292.1.
PIRiS53922.
RefSeqiNP_011715.1. NM_001181328.1.

Genome annotation databases

EnsemblFungiiYGR199W; YGR199W; YGR199W.
GeneIDi853113.
KEGGisce:YGR199W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49133 Genomic DNA. Translation: CAA88992.1.
Z72984 Genomic DNA. Translation: CAA97226.1.
BK006941 Genomic DNA. Translation: DAA08292.1.
PIRiS53922.
RefSeqiNP_011715.1. NM_001181328.1.

3D structure databases

ProteinModelPortaliP42934.
SMRiP42934.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33452. 47 interactors.
DIPiDIP-5551N.
IntActiP42934. 1 interactor.
MINTiMINT-561799.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

PTM databases

iPTMnetiP42934.

Proteomic databases

MaxQBiP42934.
PRIDEiP42934.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR199W; YGR199W; YGR199W.
GeneIDi853113.
KEGGisce:YGR199W.

Organism-specific databases

EuPathDBiFungiDB:YGR199W.
SGDiS000003431. PMT6.

Phylogenomic databases

GeneTreeiENSGT00860000133869.
HOGENOMiHOG000157526.
InParanoidiP42934.
KOiK00728.
OMAiSTWWNVE.
OrthoDBiEOG092C0JF4.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YGR199W-MONOMER.
BRENDAi2.4.1.109. 984.

Miscellaneous databases

PROiP42934.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39-like.
IPR003342. Glyco_trans_39/83.
IPR016093. MIR_motif.
IPR032421. PMT_4TMC.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
PF16192. PMT_4TMC. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMT6_YEAST
AccessioniPrimary (citable) accession number: P42934
Secondary accession number(s): D6VUY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.