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Protein

T-complex protein 1 subunit theta

Gene

Cct8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit theta
Short name:
TCP-1-theta
Alternative name(s):
CCT-theta
Gene namesi
Name:Cct8
Synonyms:Cctq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:107183. Cct8.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: MGI
  • cell-cell adherens junction Source: MGI
  • centrosome Source: MGI
  • chaperonin-containing T-complex Source: MGI
  • cilium Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • intermediate filament cytoskeleton Source: MGI
  • microtubule Source: MGI
  • nucleoplasm Source: MGI
  • zona pellucida receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001283742 – 548T-complex protein 1 subunit thetaAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei23PhosphoserineCombined sources1
Modified residuei30PhosphotyrosineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei269PhosphoserineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei318N6-acetyllysineBy similarity1
Modified residuei400N6-acetyllysineCombined sources1
Cross-linki459Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei466N6-acetyllysineBy similarity1
Modified residuei505PhosphotyrosineBy similarity1
Cross-linki534Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei537PhosphoserineBy similarity1
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP42932.
PaxDbiP42932.
PeptideAtlasiP42932.
PRIDEiP42932.

2D gel databases

REPRODUCTION-2DPAGEP42932.

PTM databases

iPTMnetiP42932.
PhosphoSitePlusiP42932.
SwissPalmiP42932.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025613.
CleanExiMM_CCT8.
ExpressionAtlasiP42932. baseline and differential.
GenevisibleiP42932. MM.

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8 (By similarity). Interacts with DYX1C1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198572. 39 interactors.
IntActiP42932. 39 interactors.
MINTiMINT-1865136.
STRINGi10090.ENSMUSP00000026704.

Structurei

3D structure databases

ProteinModelPortaliP42932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0362. Eukaryota.
ENOG410XPXR. LUCA.
GeneTreeiENSGT00550000074783.
HOVERGENiHBG103107.
InParanoidiP42932.
KOiK09500.
OMAiKYGLMAV.
OrthoDBiEOG091G05WZ.
PhylomeDBiP42932.
TreeFamiTF105699.

Family and domain databases

CDDicd03341. TCP1_theta. 1 hit.
Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012721. Chap_CCT_theta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02346. chap_CCT_theta. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHVPKAPG FAQMLKDGAK HFSGLEEAVY RNIQACKELA QTTRTAYGPN
60 70 80 90 100
GMNKMVINRL EKLFVTNDAA TILRELEVQH PAAKMIVMAS HMQEQEVGDG
110 120 130 140 150
TNFVLVFAGA LLELAEELLR IGLSVSEVIS GYEIACKKAH EILPELVCCS
160 170 180 190 200
AKNLRDVDEV SSLLRTSIMS KQYGSETFLA KLIAQACVSI FPDSGNFNVD
210 220 230 240 250
NIRVCKILGS GIYSSSVLHG MVFKKETEGD VTSVKDAKIA VYSCPFDGMI
260 270 280 290 300
TETKGTVLIK TAEELMNFSK GEENLMDAQV KAIAGTGANV IVTGGKVADI
310 320 330 340 350
ALHYANKYNI MLVRLNSKWD LRRLCKTVGA TALPKLTPPV QEEMGHCDSV
360 370 380 390 400
YLSEVGDTQV VVFKHEKEDG AISTIVLRGS TDNLMDDIER AVDDGVNTFK
410 420 430 440 450
VLTRDKRLVP GGGATEIELA KQITSYGETC PGLEQYAIKK FAEAFEAIPR
460 470 480 490 500
ALAENSGVKA NEVISKLYSV HQEGNKNVGL DIEAEVPAVK DMLEASILDT
510 520 530 540
YLGKYWAIKL ATNAAVTVLR VDQIIMAKPA GGPKPPSGKK DWDDDQND
Length:548
Mass (Da):59,555
Last modified:January 23, 2007 - v3
Checksum:i4B5265250CFF1FE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37164 mRNA. Translation: CAA85521.1.
BC009007 mRNA. Translation: AAH09007.1.
CCDSiCCDS37384.1.
PIRiJC4073.
RefSeqiNP_033970.3. NM_009840.3.
UniGeneiMm.328673.

Genome annotation databases

EnsembliENSMUST00000026704; ENSMUSP00000026704; ENSMUSG00000025613.
GeneIDi12469.
KEGGimmu:12469.
UCSCiuc007zul.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37164 mRNA. Translation: CAA85521.1.
BC009007 mRNA. Translation: AAH09007.1.
CCDSiCCDS37384.1.
PIRiJC4073.
RefSeqiNP_033970.3. NM_009840.3.
UniGeneiMm.328673.

3D structure databases

ProteinModelPortaliP42932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198572. 39 interactors.
IntActiP42932. 39 interactors.
MINTiMINT-1865136.
STRINGi10090.ENSMUSP00000026704.

PTM databases

iPTMnetiP42932.
PhosphoSitePlusiP42932.
SwissPalmiP42932.

2D gel databases

REPRODUCTION-2DPAGEP42932.

Proteomic databases

EPDiP42932.
PaxDbiP42932.
PeptideAtlasiP42932.
PRIDEiP42932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026704; ENSMUSP00000026704; ENSMUSG00000025613.
GeneIDi12469.
KEGGimmu:12469.
UCSCiuc007zul.1. mouse.

Organism-specific databases

CTDi10694.
MGIiMGI:107183. Cct8.

Phylogenomic databases

eggNOGiKOG0362. Eukaryota.
ENOG410XPXR. LUCA.
GeneTreeiENSGT00550000074783.
HOVERGENiHBG103107.
InParanoidiP42932.
KOiK09500.
OMAiKYGLMAV.
OrthoDBiEOG091G05WZ.
PhylomeDBiP42932.
TreeFamiTF105699.

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

ChiTaRSiCct8. mouse.
PROiP42932.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025613.
CleanExiMM_CCT8.
ExpressionAtlasiP42932. baseline and differential.
GenevisibleiP42932. MM.

Family and domain databases

CDDicd03341. TCP1_theta. 1 hit.
Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012721. Chap_CCT_theta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02346. chap_CCT_theta. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCPQ_MOUSE
AccessioniPrimary (citable) accession number: P42932
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.