P42929 (HSPB1_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock protein beta-1
Short name=HspB1

Alternative name(s):
Heat shock 27 kDa protein
Short name=HSP 27

Gene names

Name:	HSPB1
Synonyms:	HSP27

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in stress resistance and actin organization.
Subunit structure	Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8, HSPBAP1 and TGFB1I1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles By similarity.
Post-translational modification	Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement By similarity.
Sequence similarities	Belongs to the small heat shock protein (HSP20) family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm Cytoskeleton Nucleus
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Heat shock protein beta-1		PRO_0000125925
Regions
	Region	74 – 209	136	Interaction with TGFB1I1 By similarity
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	15	1	Phosphoserine; by MAPKAPK2 and MAPKAPK3 By similarity
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	82	1	Phosphoserine; by MAPKAPK2, MAPKAPK3 and MAPKAPK5 By similarity
	Modified residue	86	1	Phosphoserine; by MAPKAPK2, MAPKAPK3 and MAPKAPK5 By similarity
	Modified residue	87	1	Phosphoserine By similarity
	Modified residue	127	1	N6-acetyllysine By similarity
	Modified residue	203	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42929 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7E59F696D8C7F1BD	FASTA	209	22,939
	10 20 30 40 50 60 MTERRVPFSL LRSPSWDPFR DWYPAHSRLF DQAFGLPRLP EEWAQWFGHS GWPGYVRPIP 70 80 90 100 110 120 PAVEGPAAAA AAAAPAYSRA LSRQLSSGVS EIRQTADRWR VSLDVNHFAP EELTVKTKDG 130 140 150 160 170 180 VVEITGKHEE RQDEHGYISR RLTPKYTLPP GVDPTLVSSS LSPEGTLTVE APMPKPATQS 190 200 AEITIPVTFE ARAQIGGPEA GKSEQSGAK

References

[1]	"Cloning and sequencing of a cDNA encoding the canine HSP27 protein." Larsen J.K., Gerthoffer W.T., Hickey E., Weber L.A. Gene 161:305-306(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon smooth muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19368 mRNA. Translation: AAA87172.1.
PIR	JC4244.
RefSeq	NP_001003295.1. NM_001003295.1.
UniGene	Cfa.3849.
3D structure databases
ProteinModelPortal	P42929.
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000019945.
Proteomic databases
PaxDb	P42929.
PRIDE	P42929.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	403979.
KEGG	cfa:403979.
Organism-specific databases
CTD	3315.
Phylogenomic databases
eggNOG	NOG307785.
HOGENOM	HOG000233955.
HOVERGEN	HBG054766.
InParanoid	P42929.
KO	K04455.
OrthoDB	EOG4DR9DF.
Family and domain databases
InterPro	IPR002068. a-crystallin/Hsp20_dom. IPR001436. Alpha-crystallin/HSP. IPR008978. HSP20-like_chaperone. [Graphical view]
Pfam	PF00011. HSP20. 1 hit. [Graphical view]
PIRSF	PIRSF036514. Sm_HSP_B1. 1 hit.
PRINTS	PR00299. ACRYSTALLIN.
SUPFAM	SSF49764. HSP20_chap. 1 hit.
PROSITE	PS01031. HSP20. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817469.

Entry information

Entry name

HSPB1_CANFA

Accession

Primary (citable) accession number: P42929

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program