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Protein

50S ribosomal protein L2

Gene

rplB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU01190-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2UniRule annotation
Short name:
BL2
Gene namesi
Name:rplBUniRule annotation
Ordered Locus Names:BSU01190
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 27727650S ribosomal protein L2PRO_0000129529Add
BLAST

Proteomic databases

PaxDbiP42919.
PRIDEiP42919.

Interactioni

Subunit structurei

Forms a bridge to the 30S subunit in the 70S ribosome (By similarity). Part of the 50S ribosomal subunit.By similarity1 Publication

Protein-protein interaction databases

IntActiP42919. 1 interaction.
MINTiMINT-8366504.
STRINGi224308.Bsubs1_010100000615.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J3Velectron microscopy13.30C1-277[»]
3J3Welectron microscopy10.70C1-277[»]
3J9Welectron microscopy3.90BD1-277[»]
ProteinModelPortaliP42919.
SMRiP42919. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L2P family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CFD. Bacteria.
COG0090. LUCA.
HOGENOMiHOG000232982.
InParanoidiP42919.
KOiK02886.
OMAiHNRGVTM.
PhylomeDBiP42919.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SMARTiSM01383. Ribosomal_L2. 1 hit.
SM01382. Ribosomal_L2_C. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKKYKPTS NGRRGMTTSD FAEITTDKPE KSLLAPLHKK GGRNNQGKLT
60 70 80 90 100
VRHQGGGHKR QYRVIDFKRD KDGIPGRVAT VEYDPNRSAN IALINYADGE
110 120 130 140 150
KRYILAPKGI QVGTEIMSGP EADIKVGNAL PLINIPVGTV VHNIELKPGK
160 170 180 190 200
GGQLVRSAGT SAQVLGKEGK YVLVRLNSGE VRMILSACRA SIGQVGNEQH
210 220 230 240 250
ELINIGKAGR SRWKGIRPTV RGSVMNPNDH PHGGGEGRAP IGRKSPMSPW
260 270
GKPTLGFKTR KKKNKSDKFI VRRRKNK
Length:277
Mass (Da):30,274
Last modified:June 16, 2009 - v3
Checksum:iE126BCA251035A96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91T → S in AAC45959 (PubMed:9371452).Curated
Sequence conflicti9 – 91T → S in BAA08834 (PubMed:8969501).Curated
Sequence conflicti116 – 1161I → V in AAC45959 (PubMed:9371452).Curated
Sequence conflicti256 – 2561G → E in BAA08834 (PubMed:8969501).Curated
Sequence conflicti262 – 2621K → Q in BAA08834 (PubMed:8969501).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43929 Genomic DNA. Translation: AAC45959.1.
D50302 Genomic DNA. Translation: BAA08834.1.
AL009126 Genomic DNA. Translation: CAB11895.2.
PIRiF69694.
RefSeqiNP_388000.2. NC_000964.3.
WP_003225795.1. NZ_JNCM01000029.1.

Genome annotation databases

EnsemblBacteriaiCAB11895; CAB11895; BSU01190.
GeneIDi11241709.
936817.
KEGGibsu:BSU01190.
PATRICi18971747. VBIBacSub10457_0122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43929 Genomic DNA. Translation: AAC45959.1.
D50302 Genomic DNA. Translation: BAA08834.1.
AL009126 Genomic DNA. Translation: CAB11895.2.
PIRiF69694.
RefSeqiNP_388000.2. NC_000964.3.
WP_003225795.1. NZ_JNCM01000029.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J3Velectron microscopy13.30C1-277[»]
3J3Welectron microscopy10.70C1-277[»]
3J9Welectron microscopy3.90BD1-277[»]
ProteinModelPortaliP42919.
SMRiP42919. Positions 2-275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42919. 1 interaction.
MINTiMINT-8366504.
STRINGi224308.Bsubs1_010100000615.

Proteomic databases

PaxDbiP42919.
PRIDEiP42919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11895; CAB11895; BSU01190.
GeneIDi11241709.
936817.
KEGGibsu:BSU01190.
PATRICi18971747. VBIBacSub10457_0122.

Phylogenomic databases

eggNOGiENOG4105CFD. Bacteria.
COG0090. LUCA.
HOGENOMiHOG000232982.
InParanoidiP42919.
KOiK02886.
OMAiHNRGVTM.
PhylomeDBiP42919.

Enzyme and pathway databases

BioCyciBSUB:BSU01190-MONOMER.

Family and domain databases

Gene3Di2.30.30.30. 1 hit.
2.40.50.140. 1 hit.
4.10.950.10. 1 hit.
HAMAPiMF_01320_B. Ribosomal_L2_B. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR014722. Rib_L2_dom2.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac/org-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014726. Ribosomal_L2_dom3.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
PANTHERiPTHR13691. PTHR13691. 1 hit.
PfamiPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002158. Ribosomal_L2. 1 hit.
SMARTiSM01383. Ribosomal_L2. 1 hit.
SM01382. Ribosomal_L2_C. 1 hit.
[Graphical view]
SUPFAMiSSF50104. SSF50104. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR01171. rplB_bact. 1 hit.
PROSITEiPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL2_BACSU
AccessioniPrimary (citable) accession number: P42919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: September 7, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.