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Protein

Outer membrane usher protein YraJ

Gene

yraJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the yraHIJK fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Increases adhesion to eukaryotic T24 bladder epithelial cells in the absence of fim operon. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • pilus assembly Source: GO_Central
  • pilus organization Source: EcoCyc

Keywordsi

Biological processFimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7639-MONOMER

Protein family/group databases

TCDBi1.B.11.3.6 the outer membrane fimbrial usher porin (fup) family

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein YraJ
Gene namesi
Name:yraJ
Ordered Locus Names:b3144, JW3113
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12775 yraJ

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40Sequence analysisAdd BLAST40
ChainiPRO_000000933541 – 838Outer membrane usher protein YraJAdd BLAST798

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi815 ↔ 837Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP42915
PRIDEiP42915

Expressioni

Inductioni

Expression is negatively regulated by H-NS and subjected to cAMP receptor protein (CRP)-mediated catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261988, 145 interactors
STRINGi316385.ECDH10B_3317

Structurei

3D structure databases

ProteinModelPortaliP42915
SMRiP42915
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CW0 Bacteria
COG3188 LUCA
HOGENOMiHOG000111414
InParanoidiP42915
KOiK07347
OMAiPHARTEF
PhylomeDBiP42915

Family and domain databases

InterProiView protein in InterPro
IPR000015 Fimb_usher
IPR018030 Fimbrial_membr_usher_CS
IPR025949 PapC-like_C
IPR025885 PapC_N
IPR037224 PapC_N_sf
PANTHERiPTHR30451 PTHR30451, 1 hit
PfamiView protein in Pfam
PF13953 PapC_C, 1 hit
PF13954 PapC_N, 1 hit
PF00577 Usher, 1 hit
SUPFAMiSSF141729 SSF141729, 1 hit
PROSITEiView protein in PROSITE
PS01151 FIMBRIAL_USHER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQRHHQGHK RTPKQLALII KRCLPMVLTG SGMLCTTANA EEYYFDPIML
60 70 80 90 100
ETTKSGMQTT DLSRFSKKYA QLPGTYQVDI WLNKKKVSQK KITFTANAEQ
110 120 130 140 150
LLQPQFTVEQ LRELGIKVDE IPALAEKDDD SVINSLEQII PGTAAEFDFN
160 170 180 190 200
HQQLNLSIPQ IALYRDARGY VSPSRWDDGI PTLFTNYSFT GSDNRYRQGN
210 220 230 240 250
RSQRQYLNMQ NGANFGPWRL RNYSTWTRND QTSSWNTISS YLQRDIKALK
260 270 280 290 300
SQLLLGESAT SGSIFSSYTF TGVQLASDDN MLPNSQRGFA PTVRGIANSS
310 320 330 340 350
AIVTIRQNGY VIYQSNVSAG AFEINDLYPS SNSGDLEVTI EESDGTQRRF
360 370 380 390 400
IQPYSSLPMM QRPGHLKYSA TAGRYRADAN SDSKEPEFAE ATAIYGLNNT
410 420 430 440 450
FTLYGGLLGS EDYYALGIGI GGTLGALGAL SMDINRADTQ FDNQHSFHGY
460 470 480 490 500
QWRTQYIKDI PETNTNIAVS YYRYTNDGYF SFNEANTRNW DYNSRQKSEI
510 520 530 540 550
QFNISQTIFD GVSLYASGSQ QDYWGNNDKN RNISVGVSGQ QWGVGYSLNY
560 570 580 590 600
QYSRYTDQNN DRALSLNLSI PLERWLPRSR VSYQMTSQKD RPTQHEMRLD
610 620 630 640 650
GSLLDDGRLS YSLEQSLDDD NNHNSSLNAS YRSPYGTFSA GYSYGNDSSQ
660 670 680 690 700
YNYGVTGGVV IHPHGVTLSQ YLGNAFALID ANGASGVRIQ NYPGIATDPF
710 720 730 740 750
GYAVVPYLTT YQENRLSVDT TQLPDNVDLE QTTQFVVPNR GAMVAARFNA
760 770 780 790 800
NIGYRVLVTV SDRNGKPLPF GALASNDDTG QQSIVDEGGI LYLSGISSKS
810 820 830
QSWTVRWGNQ ADQQCQFAFS TPDSEPTTSV LQGTAQCH
Length:838
Mass (Da):93,616
Last modified:November 1, 1995 - v1
Checksum:iFD265CC903A269BA
GO

Sequence cautioni

The sequence AAA57947 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57947.1 Different initiation.
U00096 Genomic DNA Translation: AAC76178.1
AP009048 Genomic DNA Translation: BAE77190.1
PIRiD65104
RefSeqiNP_417613.1, NC_000913.3
WP_001355538.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76178; AAC76178; b3144
BAE77190; BAE77190; BAE77190
GeneIDi947656
KEGGiecj:JW3113
eco:b3144
PATRICifig|1411691.4.peg.3586

Similar proteinsi

Entry informationi

Entry nameiYRAJ_ECOLI
AccessioniPrimary (citable) accession number: P42915
Secondary accession number(s): Q2M966
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
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