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Protein

Outer membrane usher protein YraJ

Gene

yraJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Part of the yraHIJK fimbrial operon. Could contribute to adhesion to various surfaces in specific environmental niches. Increases adhesion to eukaryotic T24 bladder epithelial cells in the absence of fim operon. Probably involved in the export and assembly of fimbrial subunits across the outer membrane.1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • pilus assembly Source: GO_Central
  • pilus organization Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7639-MONOMER.
ECOL316407:JW3113-MONOMER.

Protein family/group databases

TCDBi1.B.11.3.6. the outer membrane fimbrial usher porin (fup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein YraJ
Gene namesi
Name:yraJ
Ordered Locus Names:b3144, JW3113
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12775. yraJ.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of the operon under classical laboratory conditions does not result in any major effect on E.coli capacity to form biofilms compared with the wild-type strain.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence analysisAdd
BLAST
Chaini41 – 838798Outer membrane usher protein YraJPRO_0000009335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi815 ↔ 837Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP42915.
PRIDEiP42915.

Expressioni

Inductioni

Expression is negatively regulated by H-NS and subjected to cAMP receptor protein (CRP)-mediated catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261988. 145 interactions.
STRINGi511145.b3144.

Structurei

3D structure databases

ProteinModelPortaliP42915.
SMRiP42915. Positions 43-837.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP42915.
KOiK07347.
OMAiTAYNENR.
OrthoDBiEOG6GJBP9.
PhylomeDBiP42915.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42915-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQRHHQGHK RTPKQLALII KRCLPMVLTG SGMLCTTANA EEYYFDPIML
60 70 80 90 100
ETTKSGMQTT DLSRFSKKYA QLPGTYQVDI WLNKKKVSQK KITFTANAEQ
110 120 130 140 150
LLQPQFTVEQ LRELGIKVDE IPALAEKDDD SVINSLEQII PGTAAEFDFN
160 170 180 190 200
HQQLNLSIPQ IALYRDARGY VSPSRWDDGI PTLFTNYSFT GSDNRYRQGN
210 220 230 240 250
RSQRQYLNMQ NGANFGPWRL RNYSTWTRND QTSSWNTISS YLQRDIKALK
260 270 280 290 300
SQLLLGESAT SGSIFSSYTF TGVQLASDDN MLPNSQRGFA PTVRGIANSS
310 320 330 340 350
AIVTIRQNGY VIYQSNVSAG AFEINDLYPS SNSGDLEVTI EESDGTQRRF
360 370 380 390 400
IQPYSSLPMM QRPGHLKYSA TAGRYRADAN SDSKEPEFAE ATAIYGLNNT
410 420 430 440 450
FTLYGGLLGS EDYYALGIGI GGTLGALGAL SMDINRADTQ FDNQHSFHGY
460 470 480 490 500
QWRTQYIKDI PETNTNIAVS YYRYTNDGYF SFNEANTRNW DYNSRQKSEI
510 520 530 540 550
QFNISQTIFD GVSLYASGSQ QDYWGNNDKN RNISVGVSGQ QWGVGYSLNY
560 570 580 590 600
QYSRYTDQNN DRALSLNLSI PLERWLPRSR VSYQMTSQKD RPTQHEMRLD
610 620 630 640 650
GSLLDDGRLS YSLEQSLDDD NNHNSSLNAS YRSPYGTFSA GYSYGNDSSQ
660 670 680 690 700
YNYGVTGGVV IHPHGVTLSQ YLGNAFALID ANGASGVRIQ NYPGIATDPF
710 720 730 740 750
GYAVVPYLTT YQENRLSVDT TQLPDNVDLE QTTQFVVPNR GAMVAARFNA
760 770 780 790 800
NIGYRVLVTV SDRNGKPLPF GALASNDDTG QQSIVDEGGI LYLSGISSKS
810 820 830
QSWTVRWGNQ ADQQCQFAFS TPDSEPTTSV LQGTAQCH
Length:838
Mass (Da):93,616
Last modified:November 1, 1995 - v1
Checksum:iFD265CC903A269BA
GO

Sequence cautioni

The sequence AAA57947.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57947.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76178.1.
AP009048 Genomic DNA. Translation: BAE77190.1.
PIRiD65104.
RefSeqiNP_417613.1. NC_000913.3.
WP_001355538.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76178; AAC76178; b3144.
BAE77190; BAE77190; BAE77190.
GeneIDi947656.
KEGGiecj:JW3113.
eco:b3144.
PATRICi32121706. VBIEscCol129921_3239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57947.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76178.1.
AP009048 Genomic DNA. Translation: BAE77190.1.
PIRiD65104.
RefSeqiNP_417613.1. NC_000913.3.
WP_001355538.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP42915.
SMRiP42915. Positions 43-837.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261988. 145 interactions.
STRINGi511145.b3144.

Protein family/group databases

TCDBi1.B.11.3.6. the outer membrane fimbrial usher porin (fup) family.

Proteomic databases

PaxDbiP42915.
PRIDEiP42915.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76178; AAC76178; b3144.
BAE77190; BAE77190; BAE77190.
GeneIDi947656.
KEGGiecj:JW3113.
eco:b3144.
PATRICi32121706. VBIEscCol129921_3239.

Organism-specific databases

EchoBASEiEB2628.
EcoGeneiEG12775. yraJ.

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP42915.
KOiK07347.
OMAiTAYNENR.
OrthoDBiEOG6GJBP9.
PhylomeDBiP42915.

Enzyme and pathway databases

BioCyciEcoCyc:G7639-MONOMER.
ECOL316407:JW3113-MONOMER.

Miscellaneous databases

PROiP42915.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities."
    Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.
    Environ. Microbiol. 12:1957-1977(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiYRAJ_ECOLI
AccessioniPrimary (citable) accession number: P42915
Secondary accession number(s): Q2M966
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The operon is cryptic under classical laboratory conditions, but is functional when constitutively expressed.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.