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Protein

Putative deaminase AgaI

Gene

agaI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains cannot grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaI in K-12 strains is not involved in the degradation of these compounds.

Caution

Was originally thought to be involved in the deamination and isomerization of D-galactosamine 6-phosphate to D-tagatofuranose 6-phosphate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Proton acceptor; for enolization stepBy similarity1
Active sitei154For ring-opening stepBy similarity1
Active sitei156Proton acceptor; for ring-opening stepBy similarity1
Active sitei161For ring-opening stepBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G7636-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative deaminase AgaICurated (EC:3.5.99.-Curated)
Gene namesi
Name:agaI
Synonyms:yraG
Ordered Locus Names:b3141, JW3110
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12772 agaI

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene does not affect growth on N-acetyl-D-galactosamine, D-galactosamine or N-acetyl-D-glucosamine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601891 – 251Putative deaminase AgaIAdd BLAST251

Proteomic databases

PaxDbiP42912
PRIDEiP42912

Interactioni

Protein-protein interaction databases

BioGridi4261594, 5 interactors
DIPiDIP-9069N
IntActiP42912, 4 interactors
STRINGi316385.ECDH10B_3314

Structurei

3D structure databases

ProteinModelPortaliP42912
SMRiP42912
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106HBN Bacteria
COG0363 LUCA
HOGENOMiHOG000064978
InParanoidiP42912
KOiK02080
OMAiDQICFFD
PhylomeDBiP42912

Family and domain databases

CDDicd01399 GlcN6P_deaminase, 1 hit
InterProiView protein in InterPro
IPR006148 Glc/Gal-6P_isomerase
IPR004547 Glucosamine6P_isomerase
IPR018321 Glucosamine6P_isomerase_CS
IPR037171 NagB/RpiA_transferase-like
PANTHERiPTHR11280 PTHR11280, 1 hit
PfamiView protein in Pfam
PF01182 Glucosamine_iso, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit
PROSITEiView protein in PROSITE
PS01161 GLC_GALNAC_ISOMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

P42912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGTASGGA SLLKEFHPVQ TLQQVENYTA LSERASEYLL AVIRSKPNAV
60 70 80 90 100
ICLATGATPL LTYHYLVEKI HQQQVDVSQL TFVKLDEWVD LPLTMPGTCE
110 120 130 140 150
TFLQQHIVQP LGLREDQLIS FRSEEINETE CERVTNLIAR KGGLDLCVLG
160 170 180 190 200
LGKNGHLGLN EPGESLQPAC HISQLDARTQ QHEMLKTAGR PVTRGITLGL
210 220 230 240 250
KDILNAREVL LLVTGEGKQD ATDRFLTAKV STAIPASFLW LHSNFICLIN

T
Length:251
Mass (Da):27,724
Last modified:November 1, 1995 - v1
Checksum:i823CB4CFBD82B8C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228498 Genomic DNA Translation: AAF81093.1
U18997 Genomic DNA Translation: AAA57944.1
U00096 Genomic DNA Translation: AAC76175.1
AP009048 Genomic DNA Translation: BAE77187.1
PIRiA65104
RefSeqiNP_417610.1, NC_000913.3
WP_001311150.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76175; AAC76175; b3141
BAE77187; BAE77187; BAE77187
GeneIDi947985
KEGGiecj:JW3110
eco:b3141
PATRICifig|511145.12.peg.3236

Similar proteinsi

Entry informationi

Entry nameiAGAI_ECOLI
AccessioniPrimary (citable) accession number: P42912
Secondary accession number(s): Q2M969
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health