Reviewed, UniProtKB/Swiss-Prot P42912 (AGAI_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative galactosamine-6-phosphate isomerase
    EC=5.3.1.-
Alternative name(s):
    Galactosamine-6-phosphate deaminase

Gene names

Name:	agaI
Synonyms:	yraG
Ordered Locus Names:	b3141, JW3110

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-galactosamine 6-phosphate + H2O = D-tagatose 6-phosphate + NH3.
Miscellaneous	In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, agaI in K-12 strains is not involved in the degradation of these compounds.
Sequence similarities	Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucosamine-6-phosphate deaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Putative galactosamine-6-phosphate isomerase		PRO_0000160189
Sites
	Active site	86	1	Proton acceptor; for enolization step By similarity
	Active site	154	1	For ring-opening step By similarity
	Active site	156	1	Proton acceptor; for ring-opening step By similarity
	Active site	161	1	For ring-opening step By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42912-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 823CB4CFBD82B8C6	FASTA	251	27,724
	10 20 30 40 50 60 MERGTASGGA SLLKEFHPVQ TLQQVENYTA LSERASEYLL AVIRSKPNAV ICLATGATPL 70 80 90 100 110 120 LTYHYLVEKI HQQQVDVSQL TFVKLDEWVD LPLTMPGTCE TFLQQHIVQP LGLREDQLIS 130 140 150 160 170 180 FRSEEINETE CERVTNLIAR KGGLDLCVLG LGKNGHLGLN EPGESLQPAC HISQLDARTQ 190 200 210 220 230 240 QHEMLKTAGR PVTRGITLGL KDILNAREVL LLVTGEGKQD ATDRFLTAKV STAIPASFLW 250 LHSNFICLIN T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli." Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W. Mol. Microbiol. 37:125-135(2000) [PubMed: 10931310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228498 Genomic DNA. Translation: AAF81093.1. U18997 Genomic DNA. Translation: AAA57944.1. U00096 Genomic DNA. Translation: AAC76175.1. AP009048 Genomic DNA. Translation: BAE77187.1.
PIR	A65104.
RefSeq	AP_003686.1. NP_417610.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9069N.
STRING	P42912.
Proteomic databases
PRIDE	P42912.
Genome annotation databases
GeneID	947985.
GenomeReviews	Gene locus JW3110 in contig AP009048_GR. Gene locus b3141 in contig U00096_GR.
KEGG	ecj:JW3110. eco:b3141.
Organism-specific databases
EchoBASE	EB2625.
EcoGene	EG12772. agaI.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG725991.
OMA	LNEPGEN.
Enzyme and pathway databases
BioCyc	EcoCyc:G7636-MON. ECOL168927:B3141-MON.
Gene expression databases
Genevestigator	P42912.
Family and domain databases
InterPro	IPR006148. Glc/Gal-6P_isomerase. IPR004547. Glucosamine6P_isomerase. IPR018321. Glucosamine6P_isomerase_CS. [Graphical view]
PANTHER	PTHR11280. NagB. 1 hit.
Pfam	PF01182. Glucosamine_iso. 1 hit. [Graphical view]
PROSITE	PS01161. GLC_GALNAC_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAI_ECOLI

Accession

Primary (citable) accession number: P42912
Secondary accession number(s): Q2M969

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 15, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)