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Protein

Putative galactosamine-6-phosphate isomerase

Gene

agaI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-galactosamine 6-phosphate + H2O = D-tagatose 6-phosphate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Proton acceptor; for enolization stepBy similarity
Active sitei154 – 1541For ring-opening stepBy similarity
Active sitei156 – 1561Proton acceptor; for ring-opening stepBy similarity
Active sitei161 – 1611For ring-opening stepBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciEcoCyc:G7636-MONOMER.
ECOL316407:JW3110-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative galactosamine-6-phosphate isomerase (EC:5.3.1.-)
Alternative name(s):
Galactosamine-6-phosphate deaminase
Gene namesi
Name:agaI
Synonyms:yraG
Ordered Locus Names:b3141, JW3110
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12772. agaI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Putative galactosamine-6-phosphate isomerasePRO_0000160189Add
BLAST

Proteomic databases

PaxDbiP42912.
PRIDEiP42912.

Interactioni

Protein-protein interaction databases

BioGridi4261594. 5 interactions.
DIPiDIP-9069N.
IntActiP42912. 4 interactions.
MINTiMINT-1228377.
STRINGi511145.b3141.

Structurei

3D structure databases

ProteinModelPortaliP42912.
SMRiP42912. Positions 22-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106HBN. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
InParanoidiP42912.
KOiK02080.
OMAiILQPACH.
OrthoDBiEOG6WX4S3.
PhylomeDBiP42912.

Family and domain databases

InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGTASGGA SLLKEFHPVQ TLQQVENYTA LSERASEYLL AVIRSKPNAV
60 70 80 90 100
ICLATGATPL LTYHYLVEKI HQQQVDVSQL TFVKLDEWVD LPLTMPGTCE
110 120 130 140 150
TFLQQHIVQP LGLREDQLIS FRSEEINETE CERVTNLIAR KGGLDLCVLG
160 170 180 190 200
LGKNGHLGLN EPGESLQPAC HISQLDARTQ QHEMLKTAGR PVTRGITLGL
210 220 230 240 250
KDILNAREVL LLVTGEGKQD ATDRFLTAKV STAIPASFLW LHSNFICLIN

T
Length:251
Mass (Da):27,724
Last modified:November 1, 1995 - v1
Checksum:i823CB4CFBD82B8C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228498 Genomic DNA. Translation: AAF81093.1.
U18997 Genomic DNA. Translation: AAA57944.1.
U00096 Genomic DNA. Translation: AAC76175.1.
AP009048 Genomic DNA. Translation: BAE77187.1.
PIRiA65104.
RefSeqiNP_417610.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76175; AAC76175; b3141.
BAE77187; BAE77187; BAE77187.
GeneIDi947985.
KEGGiecj:JW3110.
eco:b3141.
PATRICi32121700. VBIEscCol129921_3236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228498 Genomic DNA. Translation: AAF81093.1.
U18997 Genomic DNA. Translation: AAA57944.1.
U00096 Genomic DNA. Translation: AAC76175.1.
AP009048 Genomic DNA. Translation: BAE77187.1.
PIRiA65104.
RefSeqiNP_417610.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP42912.
SMRiP42912. Positions 22-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261594. 5 interactions.
DIPiDIP-9069N.
IntActiP42912. 4 interactions.
MINTiMINT-1228377.
STRINGi511145.b3141.

Proteomic databases

PaxDbiP42912.
PRIDEiP42912.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76175; AAC76175; b3141.
BAE77187; BAE77187; BAE77187.
GeneIDi947985.
KEGGiecj:JW3110.
eco:b3141.
PATRICi32121700. VBIEscCol129921_3236.

Organism-specific databases

EchoBASEiEB2625.
EcoGeneiEG12772. agaI.

Phylogenomic databases

eggNOGiENOG4106HBN. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
InParanoidiP42912.
KOiK02080.
OMAiILQPACH.
OrthoDBiEOG6WX4S3.
PhylomeDBiP42912.

Enzyme and pathway databases

BioCyciEcoCyc:G7636-MONOMER.
ECOL316407:JW3110-MONOMER.

Miscellaneous databases

PROiP42912.

Family and domain databases

InterProiIPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
[Graphical view]
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
PROSITEiPS01161. GLC_GALNAC_ISOMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli."
    Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W.
    Mol. Microbiol. 37:125-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli."
    Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr.
    Microbiology 142:231-250(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiAGAI_ECOLI
AccessioniPrimary (citable) accession number: P42912
Secondary accession number(s): Q2M969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains cannot grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaI in K-12 strains is not involved in the degradation of these compounds.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.