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Protein

Putative galactosamine-6-phosphate isomerase

Gene

agaI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Miscellaneous

In contrast to E.coli strains C and EC3132, K-12 strains cannot grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, AgaI in K-12 strains is not involved in the degradation of these compounds.

Catalytic activityi

D-galactosamine 6-phosphate + H2O = D-tagatose 6-phosphate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Proton acceptor; for enolization stepBy similarity1
Active sitei154For ring-opening stepBy similarity1
Active sitei156Proton acceptor; for ring-opening stepBy similarity1
Active sitei161For ring-opening stepBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciEcoCyc:G7636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative galactosamine-6-phosphate isomerase (EC:5.3.1.-)
Alternative name(s):
Galactosamine-6-phosphate deaminase
Gene namesi
Name:agaI
Synonyms:yraG
Ordered Locus Names:b3141, JW3110
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12772. agaI.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001601891 – 251Putative galactosamine-6-phosphate isomeraseAdd BLAST251

Proteomic databases

PaxDbiP42912.
PRIDEiP42912.

Interactioni

Protein-protein interaction databases

BioGridi4261594. 5 interactors.
DIPiDIP-9069N.
IntActiP42912. 4 interactors.
MINTiMINT-1228377.
STRINGi316385.ECDH10B_3314.

Structurei

3D structure databases

ProteinModelPortaliP42912.
SMRiP42912.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106HBN. Bacteria.
COG0363. LUCA.
HOGENOMiHOG000064978.
InParanoidiP42912.
KOiK02080.
PhylomeDBiP42912.

Family and domain databases

CDDicd01399. GlcN6P_deaminase. 1 hit.
InterProiView protein in InterPro
IPR006148. Glc/Gal-6P_isomerase.
IPR004547. Glucosamine6P_isomerase.
IPR018321. Glucosamine6P_isomerase_CS.
PANTHERiPTHR11280. PTHR11280. 1 hit.
PfamiView protein in Pfam
PF01182. Glucosamine_iso. 1 hit.
PROSITEiView protein in PROSITE
PS01161. GLC_GALNAC_ISOMERASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P42912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGTASGGA SLLKEFHPVQ TLQQVENYTA LSERASEYLL AVIRSKPNAV
60 70 80 90 100
ICLATGATPL LTYHYLVEKI HQQQVDVSQL TFVKLDEWVD LPLTMPGTCE
110 120 130 140 150
TFLQQHIVQP LGLREDQLIS FRSEEINETE CERVTNLIAR KGGLDLCVLG
160 170 180 190 200
LGKNGHLGLN EPGESLQPAC HISQLDARTQ QHEMLKTAGR PVTRGITLGL
210 220 230 240 250
KDILNAREVL LLVTGEGKQD ATDRFLTAKV STAIPASFLW LHSNFICLIN

T
Length:251
Mass (Da):27,724
Last modified:November 1, 1995 - v1
Checksum:i823CB4CFBD82B8C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF228498 Genomic DNA. Translation: AAF81093.1.
U18997 Genomic DNA. Translation: AAA57944.1.
U00096 Genomic DNA. Translation: AAC76175.1.
AP009048 Genomic DNA. Translation: BAE77187.1.
PIRiA65104.
RefSeqiNP_417610.1. NC_000913.3.
WP_001311150.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76175; AAC76175; b3141.
BAE77187; BAE77187; BAE77187.
GeneIDi947985.
KEGGiecj:JW3110.
eco:b3141.
PATRICifig|511145.12.peg.3236.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiAGAI_ECOLI
AccessioniPrimary (citable) accession number: P42912
Secondary accession number(s): Q2M969
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families