Reviewed, UniProtKB/Swiss-Prot P42911 (PTPD_ECOLI)

Names and origin

Protein names

Recommended name:
    N-acetylgalactosamine permease IID component
Alternative name(s):
    PTS system N-acetylgalactosamine-specific EIID component
    EIID-Aga

Gene names

Name:	agaD
Synonyms:	yraF
Ordered Locus Names:	b3140, JW3109

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N-acetylgalactosamine transport.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.5
Domain	The EIID domain, with its homologous EIIC domain, forms the PTS system translocation channel and contains part of its specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIID domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	N-acetylgalactosamine permease IID component		PRO_0000186662
Regions
	Transmembrane	61 – 81	21	Potential
	Transmembrane	98 – 118	21	Potential
	Transmembrane	131 – 151	21	Potential
	Transmembrane	178 – 198	21	Potential
	Transmembrane	215 – 235	21	Potential
	Transmembrane	243 – 263	21	Potential
	Domain	3 – 263	261	PTS EIID

Sequences

	Sequence		Length	Mass (Da)
	P42911-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 11573AF3503C53F1	FASTA	263	29,001
	10 20 30 40 50 60 MGSEISKKDI TRLGFRSSLL QASFNYERMQ AGGFTWAMLP ILKKIYKDDK PGLSAAMKDN 70 80 90 100 110 120 LEFINTHPNL VGFLMGLLIS MEEKGENRDT IKGLKVALFG PIAGIGDAIF WFTLLPIMAG 130 140 150 160 170 180 ICSSFASQGN LLGPILFFAV YLLIFFLRVG WTHVGYSVGV KAIDKVRENS QMIARSATIL 190 200 210 220 230 240 GITVIGGLIA SYVHINVVTS FAIDNTHSVA LQQDFFDKVF PNILPMAYTL LMYYFLRVKK 250 260 AHPVLLIGVT FVLSIVCSAF GIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli." Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W. Mol. Microbiol. 37:125-135(2000) [PubMed: 10931310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed: 8932697] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF228498 Genomic DNA. Translation: AAF81092.1. U18997 Genomic DNA. Translation: AAA57943.1. Different initiation. U00096 Genomic DNA. Translation: AAC76174.1. AP009048 Genomic DNA. Translation: BAE77186.1.
PIR	H65103.
RefSeq	AP_003685.1. NP_417609.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	4.A.6.1.5. PTS mannose-fructose-sorbose (Man) family.
Genome annotation databases
GeneID	947649.
GenomeReviews	Gene locus JW3109 in contig AP009048_GR. Gene locus b3140 in contig U00096_GR.
KEGG	ecj:JW3109. eco:b3140.
Organism-specific databases
EchoBASE	EB2624.
EcoGene	EG12771. agaD.
CMR	Search...
Phylogenomic databases
HOGENOM	P42911.
OMA	P42911. NILPMGY.
Enzyme and pathway databases
BioCyc	EcoCyc:AGAD-MON.
Family and domain databases
InterPro	IPR004704. PTS_IID_comp. IPR018405. PTS_IID_comp_subgr. [Graphical view]
Pfam	PF03613. EIID-AGA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00828. EIID-AGA. 1 hit.
PROSITE	PS51108. PTS_EIID. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTPD_ECOLI

Accession

Primary (citable) accession number: P42911
Secondary accession number(s): Q2M970

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)